breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Read File Information

read filereadsbasespassed filtersaveragelongestmapped
errorsSNFR_3_21_1_S181_S2006_L006_R1_001.good.fq913,798127,123,150100.0%139.1 bases141 bases90.5%
errorsSNFR_3_21_1_S181_S2006_L006_R2_001.good.fq913,798127,123,150100.0%139.1 bases141 bases89.6%
total1,827,596254,246,300100.0%139.1 bases141 bases90.0%

Reference Sequence Information

seq idlengthfit meanfit dispersion% mapped readsdescription
coveragedistributionCP0007302,872,91579.01.8100.0%Staphylococcus aureus subsp. aureus USA300_TCH1516, complete genome.
total2,872,915100.0%

fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.

New Junction Evidence

Junction Candidates Tested

optionlimitactual
Number of alignment pairs examined for constructing junction candidates≤ 10000056483
Coverage evenness (position-hash) score of junction candidates≥ 2≥ 3
Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold100 ≤ n ≤ 5000146
Total length of all junction candidates (factor times the reference genome length)≤ 0.10.015

Junction Skew Score Calculation

reference sequencepr(no read start)
CP0007300.77784

pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.

Final Junction Predictions

optionvalue
Coverage evenness (position-hash) score of predicted junctions must be≥ 3
Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be≤ 3
Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction≥ 1

Read Alignment Evidence

optionvalue
ModeConsensus/Mixed Base
Ploidy1 (haploid)
Consensus mutation E-value cutoff10
Consensus frequency cutoff0.8
Consensus minimum variant coverage each strandOFF
Consensus minimum total coverage each strandOFF
Consensus minimum variant coverageOFF
Consensus minimum total coverageOFF
Polymorphism E-value cutoff10
Polymorphism frequency cutoff0.2
Polymorphism minimum variant coverage each strandOFF
Polymorphism minimum total coverage each strandOFF
Polymorphism minimum variant coverageOFF
Polymorphism minimum total coverageOFF
Polymorphism bias cutoffOFF
Predict indel polymorphismsYES
Skip indel polymorphisms in homopolymers runs ofOFF
Skip base substitutions when they create a homopolymer flanked on each side byOFF

Software Versions

programversion
bowtie22.3.4.3
R3.5.1

Execution Times

stepstartendelapsed
Read and reference sequence file input22:33:56 29 Oct 201822:34:31 29 Oct 201835 seconds
Read alignment to reference genome22:34:31 29 Oct 201822:38:28 29 Oct 20183 minutes 57 seconds
Preprocessing alignments for candidate junction identification22:38:28 29 Oct 201822:38:56 29 Oct 201828 seconds
Preliminary analysis of coverage distribution22:38:56 29 Oct 201822:39:43 29 Oct 201847 seconds
Identifying junction candidates22:39:43 29 Oct 201822:40:12 29 Oct 201829 seconds
Re-alignment to junction candidates22:40:12 29 Oct 201822:41:12 29 Oct 20181 minute 0 seconds
Resolving best read alignments22:41:12 29 Oct 201822:42:01 29 Oct 201849 seconds
Creating BAM files22:42:01 29 Oct 201822:42:42 29 Oct 201841 seconds
Tabulating error counts22:42:42 29 Oct 201822:42:58 29 Oct 201816 seconds
Re-calibrating base error rates22:42:58 29 Oct 201822:42:59 29 Oct 20181 second
Examining read alignment evidence22:42:59 29 Oct 201822:45:13 29 Oct 20182 minutes 14 seconds
Polymorphism statistics22:45:13 29 Oct 201822:45:14 29 Oct 20181 second
Output22:45:14 29 Oct 201822:45:20 29 Oct 20186 seconds
Total 11 minutes 24 seconds