Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	1,744,544	T→C	Y43C (TAC→TGC)	apt ←	adenine phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,744,544	0	T	C	100.0%	101.8 / NA	29	Y43C (TAC→TGC)	apt	adenine phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/11);  total (18/11)

ATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTC  >  CP000730/1744409‑1744680                                                                                                                                        |                                                                                                                                         tggttgtatacaGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCt                                                                                                                                     <  2:128910/130‑1 (MQ=255) aTGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCt                                                                                                                                     >  2:101764/1‑141 (MQ=255)               tAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAAtt                                                                                                                               >  2:321215/1‑133 (MQ=255)               tAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAAtt                                                                                                                               <  1:321215/133‑1 (MQ=255)                     cccTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATa                                                                                                                 <  1:268786/141‑1 (MQ=255)                                tAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCtt                                                                                                      <  1:381908/141‑1 (MQ=255)                                    aGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCAcc                                                                                                  >  1:317551/1‑141 (MQ=255)                                        gCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTa                                                                                              <  2:45142/141‑1 (MQ=255)                                           aaGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATcc                                                                                           <  1:133890/141‑1 (MQ=255)                                           aaGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCCCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATcc                                                                                           <  1:82282/141‑1 (MQ=255)                                               cAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATaa                                                                                       >  2:47423/1‑141 (MQ=255)                                                       aTTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTgtagta                                                                               >  1:146977/1‑141 (MQ=255)                                                               aGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCtt                                                                       >  2:15230/1‑141 (MQ=255)                                                                    cAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAAc                                                                  >  2:13590/1‑141 (MQ=255)                                                                            ccAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCAATAATTGTAGTAATATCCTTGAAACTAACACCt                                                          <  2:195943/141‑1 (MQ=255)                                                                            ccAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATATCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCt                                                          >  1:320475/1‑141 (MQ=255)                                                                                tGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGtt                                                      >  2:229648/1‑141 (MQ=255)                                                                                   tAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCg                                                   >  2:293393/1‑141 (MQ=255)                                                                                    aaaGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCgg                                                  <  2:277258/141‑1 (MQ=255)                                                                                    aaaGCCACGCGCCTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTTTCCATAATTGTAGTAATATCCTTTACACTAACACCTGGTTTCgg                                                  <  2:36747/141‑1 (MQ=255)                                                                                      aGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTCACACCTGGTTTCGGcc                                                >  1:136042/1‑141 (MQ=255)                                                                                       gCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACATTTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCa                                               >  1:182222/1‑141 (MQ=255)                                                                                       gCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCa                                               >  2:313631/1‑141 (MQ=255)                                                                                                                atatCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGc                      <  1:180442/141‑1 (MQ=255)                                                                                                                  atCAACATCTCTGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCtt                    >  2:111623/1‑141 (MQ=255)                                                                                                                          ctctGTCTTTTGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCa            >  1:233411/1‑141 (MQ=255)                                                                                                                             tGTCTTTTGTGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTa         >  2:299564/1‑141 (MQ=255)                                                                                                                                 ttttGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAatat     >  2:216732/1‑141 (MQ=255)                                                                                                                                    tGCGCATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTtc  >  1:35058/1‑141 (MQ=255)                                                                                                                                        |                                                                                                                                         ATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTC  >  CP000730/1744409‑1744680

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑