BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,269	C→T	61.3%	A95T (GCA→ACA)	tpn ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,269	0	C	T	61.3%	466.3 / inf	1181	A95T (GCA→ACA)	tpn	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base C (229/225); new base T (332/384); total (565/616)
Fisher's exact test for biased strand distribution p-value = 1.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1197 total aligned reads displayed.

TGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGT  >  CP000730/36129‑36409

 gCTACCTTCGTTGAAGGTGCTTGATCTGTAATTACCTTTTGAGGTTTACCAAAGTGTTTAATGAGACGTTTGATAATCGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCa                                                                                                                                             <  1:142628/141‑1 (MQ=12)

  gttgcttgtgtGAAGGGGCCTGAGTGGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCAt                                                                                                                                            <  2:844356/131‑1 (MQ=11)

     actgtgtggAATGTGCCTTATCTGTAATTACCTTTTGAGGTTTAATAAATAATTTTATGAGACGTTTGATAAACGCATATGCTTAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCaa                                                                                                                                         <  1:261264/133‑1 (MQ=11)

      cTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAAt                                                                                                                                        <  2:88888/141‑1 (MQ=17)

        tCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATAATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATgg                                                                                                                                      >  1:381986/1‑141 (MQ=255)

          gTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCa                                                                                                                                    <  2:78239/141‑1 (MQ=255)

           ttGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCAc                                                                                                                                   >  1:244375/1‑141 (MQ=32)

                   gCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATaaa                                                                                                                           <  2:204649/141‑1 (MQ=12)

                    ccTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATaaaa                                                                                                                          >  2:408594/1‑140 (MQ=17)

                    ccTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGATAATGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATaaaa                                                                                                                          >  1:493110/1‑140 (MQ=12)

                      tGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAg                                                                                                                        >  2:757648/1‑141 (MQ=255)

                       gATCTGGAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGc                                                                                                                       <  1:909453/141‑1 (MQ=17)

                        aTCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCt                                                                                                                      <  1:618540/141‑1 (MQ=17)

                           tGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCa                                                                                                                   <  2:257585/141‑1 (MQ=17)

                               aTTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGATTACGCAACCAAATATCTAATGTATGTCCCTCTGGATCAATGGGACGATATAAATAGCGCCActtt                                                                                                               >  2:913769/1‑141 (MQ=11)

                                   ccttgtgactcttACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATGTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCtt                                                                                                           <  1:633671/130‑1 (MQ=255)

                                   ccTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCtt                                                                                                           <  1:403071/141‑1 (MQ=32)

                                   ccTTTTGAGGTGTACCAAATTGGTTAATGAGACGTTTGATACACGCAGATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATCTCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCtt                                                                                                           <  2:166249/141‑1 (MQ=11)

                                    ccttggAGGCTTACCAATTTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCttt                                                                                                          <  1:716951/136‑1 (MQ=11)

                                    cTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCCTATGCTGAATGATTATCTCGTTGCTTACGCAACCACATATCTAATGTATGTCCCTCTGTATCCATGCCCCGATATAAAACGCCCCCTTGTCCttt                                                                                                          >  2:128111/1‑141 (MQ=11)

                                    cTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCttt                                                                                                          <  2:79958/141‑1 (MQ=17)

                                    cTTGTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCttt                                                                                                          <  2:674392/141‑1 (MQ=12)

                                      tttGAGGATGAGCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGACTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTa                                                                                                        <  1:216256/141‑1 (MQ=255)

                                        ttatgTTTTCCAAATTTTTTTATTAGACGTTTGATAATCGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTAtt                                                                                                      <  1:19026/137‑1 (MQ=11)

                                        tGAGTTTTACCAAATTGGTTAATGAGACGTTTGATAACCGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCCATGGCACGATATAAATAGCTCCATTTTCCGTTTAtt                                                                                                      <  2:338757/141‑1 (MQ=11)

                                        tGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTAtt                                                                                                      >  1:891033/1‑141 (MQ=17)

                                        tGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTAtg                                                                                                      <  2:722140/141‑2 (MQ=12)

                                         gAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTAttt                                                                                                     >  1:198621/1‑141 (MQ=17)

                                               tACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGAtat                                                                                               <  2:59974/141‑3 (MQ=17)

                                                   aaaTTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATTTACGt                                                                                           >  1:811426/1‑141 (MQ=17)

                                                     aTTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGtct                                                                                         >  2:767921/1‑141 (MQ=17)

                                                     aTTGTTTAATGAGACGATTGCAAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGtct                                                                                         >  1:457591/1‑141 (MQ=12)

                                                      ttGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTTATATACGtctc                                                                                        >  2:400709/1‑141 (MQ=17)

                                                        gTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCtcat                                                                                      <  1:764302/141‑1 (MQ=32)

                                                         tttAAGGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCtcatc                                                                                     <  1:336029/141‑1 (MQ=12)

                                                            aaTGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAACTATCTAATGAATGTCCAACTGCATCAATGGCACGATATAAATGGCGCCATTTTCCTTTTATTTTGATGTACGTCTCATCaaa                                                                                  >  2:383794/1‑140 (MQ=11)

                                                             aTGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTGTCATCAATa                                                                                 >  1:367354/1‑141 (MQ=17)

                                                             aTGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTACTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATa                                                                                 >  1:734746/1‑141 (MQ=12)

                                                               gagaCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACg                                                                               <  2:559631/141‑1 (MQ=32)

                                                                   cGTTTGATATACGTATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCAt                                                                           <  1:876078/141‑1 (MQ=255)

                                                                   cGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCAt                                                                           <  2:148326/141‑1 (MQ=32)

                                                                    gTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCAtt                                                                          >  2:872445/1‑141 (MQ=17)

                                                                    gTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCAtt                                                                          <  1:670406/141‑1 (MQ=32)

                                                                    gTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCAtt                                                                          <  1:161418/141‑1 (MQ=32)

                                                                      ttGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTCATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTACATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTg                                                                        >  2:186962/1‑141 (MQ=255)

                                                                        gATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGta                                                                      <  2:85025/141‑1 (MQ=32)

                                                                         aTAATCGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAATTAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGtaa                                                                     <  1:101587/141‑1 (MQ=17)

                                                                         aTAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGtaa                                                                     >  2:356961/1‑141 (MQ=32)

                                                                           aaaCGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTTCATCAATGTCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGGCTCATCAATCAGCCATTTTtgata                                                                   >  1:410920/1‑141 (MQ=11)

                                                                           aaaCGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGtaata                                                                   <  1:855893/141‑1 (MQ=16)

                                                                             aCGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAACTATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCATTCCGCCATTTGTAATAAg                                                                 >  1:297182/1‑141 (MQ=11)

                                                                              cGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCC‑TTTATTTTGATATCCGTCTCATCAATACGCCATTTGtaataccct                                                               >  1:426879/1‑137 (MQ=11)

                                                                               gCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCt                                                               >  1:656243/1‑141 (MQ=25)

                                                                                  tatGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCtttt                                                            >  2:542640/1‑141 (MQ=21)

                                                                                    tGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCtttttt                                                          <  1:590457/141‑1 (MQ=35)

                                                                                    tGCTGAATGATTATCTCGTTGCTTACGCAAACAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGTCATTTGTAATAAGCtttttt                                                          <  1:122715/141‑1 (MQ=255)

                                                                                      cTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCtttttttt                                                        >  1:475160/1‑139 (MQ=25)

                                                                                       tGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTTTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCATTACGCCATTTGTAATAAGCTTTTTTATg                                                       <  2:301466/141‑1 (MQ=255)

                                                                                        gAATTATTATCTCGTTGCTTACGCAACCAAAAATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGc                                                      <  1:507597/141‑1 (MQ=255)

                                                                                        gAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGc                                                      >  1:558707/1‑141 (MQ=39)

                                                                                          aTGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCtt                                                    >  2:691818/1‑141 (MQ=40)

                                                                                            gATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCtttt                                                  <  1:747273/141‑1 (MQ=255)

                                                                                              ttATCTCGTCGCTTACGCAACCAAATATCTAATGTATGTCCCTCGGTATCAATGGCATGATATAAATAGCTCCATGTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttc                                                <  1:315780/141‑1 (MQ=14)

                                                                                               tATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttct                                               <  1:525962/141‑1 (MQ=40)

                                                                                               tATCTCGTTGCATACTCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGTTGTACGTCTCATCAAGACGCCATTTGTAATAAGCTTTTTTATGCTTTttct                                               <  1:838977/141‑1 (MQ=255)

                                                                                                aTCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATCCGCCATTTGTAATAAGCTTTTTTATGCTTTttctt                                              >  2:829416/1‑141 (MQ=255)

                                                                                                 tctcGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttcttc                                             <  1:228065/141‑1 (MQ=255)

                                                                                                 tcgcGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttcttc                                             <  2:741925/138‑1 (MQ=255)

                                                                                                  ttcGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTcc                                            <  2:424596/140‑1 (MQ=39)

                                                                                                  ctcGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTcc                                            >  2:52774/1‑141 (MQ=255)

                                                                                                     gTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATAAGTCTCATCATTACGCCATGAGGAATAAGCTTTTTTCTGCTTTGTCTTCCtaa                                         >  1:543558/1‑141 (MQ=14)

                                                                                                     gTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCcaa                                         >  2:487062/1‑141 (MQ=255)

                                                                                                        gCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAAttt                                      <  2:475160/141‑1 (MQ=40)

                                                                                                         cTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTg                                     >  2:636013/1‑141 (MQ=255)

                                                                                                             cGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTTTTTTGATATACGTCTCATCAATACGCCATTTGTAATAAACTTTTTTATGCTTTTTTTTCAAAATTTGATaa                                 >  1:324286/1‑140 (MQ=14)

                                                                                                             cGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAACCTTTTTTATGCTTTTTCTTCCAAATTTGTTat                                 >  2:218683/1‑140 (MQ=255)

                                                                                                             cGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCCTTGGTCATAACCTTTTTTATGCTTTTTTTTCCGAATTTGTTat                                 >  2:58372/1‑140 (MQ=14)

                                                                                                             cGCAACCAAATATCTAATGTATGCCCCTCGCTAACAATGTCACCATATATATTGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATat                                 <  1:357800/141‑2 (MQ=14)

                                                                                                              gCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATata                                >  2:446409/1‑139 (MQ=38)

                                                                                                              ccAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATata                                <  1:919900/140‑3 (MQ=255)

                                                                                                               cAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATaa                               <  1:939140/141‑1 (MQ=255)

                                                                                                                aaCCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAGTAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTATTATTCTTTTTCTTCCAAATTTGATACcaa                              >  2:614631/1‑141 (MQ=255)

                                                                                                                 aCCAAATATCTAATGTATGTCCTTCTGCATCAACGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATaaaa                             <  1:281322/141‑1 (MQ=255)

                                                                                                                     aaTATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTTATAAATTGACCATTGGTAATAAGCTTTTTTATGCTTTTTCTTTCACATTTGATATAAAATTgg                         >  1:447450/1‑141 (MQ=11)

                                                                                                                      atatCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGTTTTACGTCTTATCAATACGCCATTTGTAAATAGCTTTTTTATGCTTTTTCTTGCAAATTTgtgataactttggg                        >  1:6070/1‑128 (MQ=11)

                                                                                                                         tCTTATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCa                     <  1:580580/141‑1 (MQ=255)

                                                                                                                         tCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGTTCCATTTTCCTTTTCTTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCa                     <  1:177424/141‑1 (MQ=11)

                                                                                                                         tCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCa                     <  2:656243/141‑1 (MQ=255)

                                                                                                                          ttAATGTTTGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCat                    <  2:274104/140‑1 (MQ=255)

                                                                                                                          cTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTGTCCTTTTCTTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCat                    <  1:695391/141‑1 (MQ=255)

                                                                                                                              tgtatgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTc                <  2:814555/141‑1 (MQ=255)

                                                                                                                               gtatgtCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTACTGCTTTTTCTTCCAAATTTGTTACAAAATTGGGGCAtcttct               >  2:104841/1‑141 (MQ=255)

                                                                                                                                tatgtCCCTATGCATCAATGGTACTATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCtt              <  1:113628/141‑1 (MQ=255)

                                                                                                                                  tgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCCTCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAACTTTGATATAAAATTGGGGCATATTCTTGa            >  2:373535/1‑141 (MQ=11)

                                                                                                                                  tgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGa            <  2:937855/141‑1 (MQ=255)

                                                                                                                                  tggacccctGTATCAATGGGACGATGTAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGa            <  2:237741/134‑1 (MQ=11)

                                                                                                                                   gtCCCTCTGTATCAATGGCACGATATAAATAGCTCCCTTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGaa           <  1:784503/141‑1 (MQ=255)

                                                                                                                                    tCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAAc          >  1:800359/1‑141 (MQ=21)

                                                                                                                                     cccTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTCATAAGCTTTTTTATGCTTTTTCTTCCAAATTTCATATAAAATTTGGGAGTCTTCTTGAAcc         >  2:459870/1‑141 (MQ=11)

                                                                                                                                     cccTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATCTTCTTGAAcc         >  2:517656/1‑141 (MQ=21)

TGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGT  >  CP000730/36129‑36409

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`