Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 36,444 C→T 50.9% R36R (AGG→AGA)  tpn ← IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073036,4440CT50.9% 33.0 / inf 421R36R (AGG→AGA) tpnIS431mec transposase
Reads supporting (aligned to +/- strand):  ref base C (109/96);  new base T (104/110);  total (214/207)
Fisher's exact test for biased strand distribution p-value = 3.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.12e-01

Only 100 of 435 total aligned reads displayed.

TTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATT‑TGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGAT‑GAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTAT  >  CP000730/36304‑36584
                                                                                                                                              |                                                                                                                                            
  tATTTTGATTTACGTCTCATTCATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATT‑TGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGAT‑GAACGTTTACACCACGTTCCCt                                                                                                                                            <  2:157611/141‑1 (MQ=255)
  tATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATT‑TGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGAT‑GAACGTTTACACCACGTTCCCt                                                                                                                                            <  2:19411/141‑1 (MQ=40)
   tttttGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATT‑TGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGAT‑GAACGTTTACACCACGTTCCCtt                                                                                                                                           <  2:613062/140‑1 (MQ=18)
   atttttctatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCACATT‑TGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTACACCACGTTCTCtt                                                                                                                                           <  2:474334/132‑1 (MQ=11)
   atcttGATATACGTCTCATCAATACGCCATTTGTAATAATCGTTTTTATGCTTTTTCTTCCAAATT‑TGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTACACCACGTTCTCtt                                                                                                                                           <  2:317672/138‑1 (MQ=11)
   aTTTTGATGTACGTCTCCTCAATACGCCATTTGTAATAAGATTTTTTCTGGAGCGT‑GGCCATTTTACTATGTGACATTGGGGCCTATTATATAGCCCTTCGGTAGACCGTTGAATGAT‑GAACGTTTACACCACGTTCCCtt                                                                                                                                           <  2:664681/141‑1 (MQ=2)
   aTTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATT‑TGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGAT‑GAACGTTTACACCACGTTCCCtt                                                                                                                                           <  1:945737/141‑1 (MQ=18)
   aTTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATT‑TGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTACACCACGTTCTCtt                                                                                                                                           <  1:60819/141‑1 (MQ=255)
   aTTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATT‑TGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTACACCACGTTCTCtt                                                                                                                                           <  1:808264/141‑1 (MQ=255)
    ttttGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATT‑TGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTACACCACGTTCTCTTa                                                                                                                                          <  1:533616/141‑1 (MQ=255)
    ttttGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATT‑TGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTACACCACGTTCTCTTa                                                                                                                                          <  1:365470/141‑1 (MQ=255)
    ttttGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATT‑TGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTGTCACCACGTTCTCTTa                                                                                                                                          <  1:471147/141‑1 (MQ=11)
     tttGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATT‑TGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGAT‑GAACGTTTACACCACGTTCCCTTaa                                                                                                                                         <  1:518628/141‑1 (MQ=18)
     tttGATATACGTCTCATCAATACGCCTTTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATT‑TGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCCTT‑AATGGTGGAACTTTTACACCACATTCTCTTaa                                                                                                                                         <  1:692991/141‑1 (MQ=11)
     tttGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATT‑TGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTACACCACGTTCTCTTaa                                                                                                                                         <  2:384941/141‑1 (MQ=11)
     tttGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATT‑TGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTACACCACGTTCTCTTaa                                                                                                                                         <  1:840460/141‑1 (MQ=11)
       tGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATTTTTTTTCTTCCAAATT‑TGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTACACCACGTTCTCTTAata                                                                                                                                       >  1:497421/1‑141 (MQ=11)
        gttGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATT‑TGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGAT‑GAACGTTTACACCACGTTCCCTTAatat                                                                                                                                      <  1:714682/139‑1 (MQ=18)
        gatatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATT‑TGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTACGCCACGTTCTCTTAatat                                                                                                                                      >  1:567388/5‑141 (MQ=11)
        gatatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATT‑TGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTACACCACGTTCTCTTAatat                                                                                                                                      >  1:592188/5‑141 (MQ=255)
        gatatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATT‑TGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTACACCACGTTCTCTTAatat                                                                                                                                      >  1:678116/5‑141 (MQ=255)
        gatatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATT‑TGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTACACAACGTTCTCTTActat                                                                                                                                      >  2:483229/5‑141 (MQ=11)
        gatatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATT‑TGATATAAAATTGGGGCATATTCTTGAACCCAACGGCGGATCATAGGATGAT‑GAACGTTTATACCACGGTCTCTTAatat                                                                                                                                      >  1:726337/5‑141 (MQ=11)
        gATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATT‑TGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGAT‑GAACGTTTACACCACGTTCCCTTAatat                                                                                                                                      >  1:642778/1‑141 (MQ=25)
          tatACGTCTCATCAATCCGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATT‑TGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTACACCACGTTCTCTTAATAttt                                                                                                                                    <  1:40809/139‑1 (MQ=11)
          tatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATT‑TGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTACACCACGTTCTCTTAATAttt                                                                                                                                    <  1:586102/139‑1 (MQ=255)
            tACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATT‑TGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGAT‑GAACGTTTACACCACGTTCCCTTAATATTTCa                                                                                                                                  >  1:619749/1‑141 (MQ=21)
            tACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATT‑TGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGAT‑GAACGTTTACACCACGTTCCCTTAATATTTCa                                                                                                                                  <  2:11951/141‑1 (MQ=21)
            aaCGTCTCATCAATCTGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATT‑TGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTACACCACGTTCTCTTAATATTTCa                                                                                                                                  <  1:506394/140‑1 (MQ=11)
             aCGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATT‑TGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGAT‑GAACGTTTACACCACGTTCCCTTAATATTTCaa                                                                                                                                 >  2:439776/1‑140 (MQ=21)
              cGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAGTT‑TGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTACCCCACGTTCTCTTAATATTTCAGa                                                                                                                                >  1:704833/1‑141 (MQ=11)
              cGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATT‑TGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTACACCACGTTCTCTTAATATTTCAGa                                                                                                                                >  2:874259/1‑141 (MQ=255)
               gTCTTATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATT‑TGATACAAAATTGGGGCATATTCTTGAACCCAATGGTAGACCGTTGAATGAT‑GAACGTTTACACCACGTTCCCTTAATATTTCAGat                                                                                                                               <  1:632752/141‑1 (MQ=255)
               gTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATT‑TGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTACACCACGTTCTCTTAATATTTCAGat                                                                                                                               <  1:111125/141‑1 (MQ=255)
               gTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATT‑TGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTACACCACGTTCTCTTAATATTTCAGat                                                                                                                               >  2:553276/1‑141 (MQ=255)
                tctcATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTTTTTTATTTT‑TGATATAAAATTAGGACATATTCTTGAACCCAATGGTAGATTGTTGAATGAT‑GACCGTTTACAGCACGTTCTCTTAATATTTCAGatt                                                                                                                              >  2:531919/1‑140 (MQ=11)
                tctcATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATT‑TGATATAAAATTGGGGCATATTCTTGCACCCAACGGTAGACCGTTGAATGAT‑GAACGTTTACACCACGTTCACTTAATCTTTCAGatt                                                                                                                              >  2:896683/1‑140 (MQ=255)
                  tcatcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATT‑TGATATAAAATTGGGGCATATTCTTGATTGATATGGGAGACCGTTGAATGAT‑GACCGTTTACACCACGTTCCCTTAATATTTCAGatata                                                                                                                            >  1:217618/1‑141 (MQ=11)
                  ccatcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATT‑TGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTACACCACGTTCTCTTAATATTTCAGatata                                                                                                                            <  1:3140/140‑1 (MQ=255)
                   catcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATT‑TGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTACACCACGTTCTCTTaa                                                                                                                                         <  2:757237/127‑1 (MQ=255)
                   catcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATT‑TGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTACACCACGTTCTCTTaa                                                                                                                                         >  1:757237/1‑127 (MQ=255)
                   catcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATT‑TGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTACACCACGTTCTCTTAATATTTCAGatatat                                                                                                                           >  2:341922/1‑141 (MQ=255)
                    atcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATT‑TGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTACACCACGTTCTCTTAATATTTCAGatatatt                                                                                                                          >  2:625123/1‑140 (MQ=255)
                    atcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATT‑TGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTACACCACGTTCTCTTAATATTTCAGATATATc                                                                                                                          >  2:150019/1‑141 (MQ=255)
                    atcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATT‑TGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTACACCACGTTCTCTTAATATTTCAGATATATc                                                                                                                          >  2:370814/1‑141 (MQ=255)
                     tcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTTTTTTTTTTT‑TGATATAAAATTGGGGCATATTCTTGAACCCAACGGTTGATCGGTGAATGAT‑GAACGTTTACACCACGTTCTCTTAATATTTCAGatctataa                                                                                                                         >  2:247204/1‑139 (MQ=11)
                     tcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATT‑TGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTACACCACGTTCTCTTAATGTTTCAGATATATCa                                                                                                                         >  1:24390/1‑141 (MQ=255)
                     tcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATT‑TGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCa                                                                                                                         >  1:783522/1‑141 (MQ=255)
                     tcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATT‑TGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTACACCACGTTCTCTTAATATTTCACATATATCa                                                                                                                         >  2:791929/1‑141 (MQ=255)
                      caATACGTCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATT‑TGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCAc                                                                                                                        <  2:56714/141‑1 (MQ=255)
                      caATACGCCATTTGTAATAAGCTTTTTTTTTTTTTTTTTTCCAAATT‑TGATATAAAATTATACGATATTCTTTAGCCGAATGGTAGATGGTTTCTTGCT‑AAACGTTTACACCACATTCTCTTAATtttttgaatttatttt                                                                                                                        >  2:580693/1‑129 (MQ=2)
                        aTACGCCATTTGTAATACGCTCCAATAGTCTGTTTCTTCCAAATT‑TGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGa                                                                                                                      >  2:910567/1‑141 (MQ=11)
                        aTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATT‑TGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGa                                                                                                                      >  1:415653/1‑141 (MQ=255)
                        aTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATT‑TGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGAT‑GAACGTTTACACTTTGCCTATTTAATATTTCAGATATATCACGa                                                                                                                      <  1:935937/141‑1 (MQ=14)
                         tACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATT‑TGATGCAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGCTGAATGAT‑GAACGGTTATGCCACGCTCCCTTAATATTTCAGagatatagagac                                                                                                                     >  1:387371/1‑135 (MQ=14)
                         tACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATT‑TGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGAt                                                                                                                     >  2:852918/1‑141 (MQ=255)
                         tACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATT‑TGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTACACCACGTTCTCTTAATATTTCAGATAAATCACGAt                                                                                                                     >  2:398530/1‑141 (MQ=255)
                         tACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATT‑TGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGAT‑GAACGTTTGCACCACGTTCCCTTAATATTTCAGATATATCACGAt                                                                                                                     >  1:173752/1‑141 (MQ=21)
                           cGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATT‑TGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATag                                                                                                                   >  2:677014/1‑140 (MQ=255)
                           cGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATT‑TGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGAT‑GAACGTTTACACCACGTTCCATTACTATTTCAGATATATCACGATaa                                                                                                                   >  2:763279/1‑141 (MQ=255)
                           cGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATT‑TGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGAT‑GAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATaa                                                                                                                   >  1:767594/1‑141 (MQ=40)
                             ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATT‑TGATGTAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGAT‑GAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACt                                                                                                                 >  2:514524/1‑141 (MQ=25)
                             ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATT‑TGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCt                                                                                                                 >  2:828577/1‑141 (MQ=255)
                              cATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATT‑TGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTc                                                                                                                <  1:340647/141‑1 (MQ=11)
                              cATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATT‑TGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGAT‑GAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTc                                                                                                                >  1:66601/1‑141 (MQ=21)
                              cATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATT‑TGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGAT‑GAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTc                                                                                                                <  1:794981/141‑1 (MQ=21)
                               ttttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATT‑TGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCa                                                                                                               <  2:499867/140‑1 (MQ=255)
                               aTTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATT‑TGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTACACCACGTTCTCTTAATATTTCAGATACATCACGATAGCTCa                                                                                                               >  2:715367/1‑141 (MQ=11)
                               aTTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATT‑TGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGAT‑GAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCa                                                                                                               <  1:828474/141‑1 (MQ=21)
                               aTTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATT‑TGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGAT‑GAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCa                                                                                                               <  2:740273/141‑1 (MQ=18)
                               aTTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATT‑TGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGAT‑GAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCa                                                                                                               <  1:904008/141‑1 (MQ=18)
                                tttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATT‑TGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCaa                                                                                                              >  2:35773/1‑141 (MQ=11)
                                tttGTAATAAGCGTTTTTATGCTTTTTCTTCCAACTT‑TGATATAAAATTGGGGCATATTTTTGAACCCAACGGTAGACCGTTGAATGAT‑GTACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCaa                                                                                                              <  2:941906/141‑1 (MQ=11)
                                 ttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATT‑TGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAAt                                                                                                             <  2:297182/141‑1 (MQ=11)
                                  tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATT‑TGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCaagg                                                                                                            >  1:846773/1‑139 (MQ=11)
                                  tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATT‑TGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATg                                                                                                            <  2:223622/141‑1 (MQ=255)
                                  tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATT‑TGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATg                                                                                                            >  1:454022/1‑141 (MQ=255)
                                  tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATT‑TGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTACACCACGTTCTCTTAAGATTTCAGATATATCACGATAGCTCAATg                                                                                                            <  1:298819/141‑1 (MQ=11)
                                   gTAATAAGGTTTTTTATGCTTTTTCTTCCAAATT‑TGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGAT‑GAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATgc                                                                                                           <  2:643960/141‑2 (MQ=17)
                                   gTAATAAGCTTTTTTATGCTTTTGCTTCCAAATT‑TGATATAAAATGAGGGCATGTTCTTGAACCCAACGGTTGCTCGATCAATGAT‑GAAAGCTTACACCACGATCTCTTACTCTTTAAGATATATCACGATAGCTCactcc                                                                                                           >  2:185216/1‑137 (MQ=2)
                                    taataaGCTTTTTTATGCTTTTTCTTCCAAATT‑TGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGAT‑GAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGta                                                                                                          >  1:429159/1‑141 (MQ=255)
                                    taataaGCTTTTTTATGCTTTTTCTTCCAAATT‑CGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGAT‑GAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATgca                                                                                                          <  1:377304/141‑3 (MQ=12)
                                                  aTGCTTTTTCTTCCAAATT‑TGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGCATGAT‑GAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGAtagta                                                                                            >  1:868803/1‑141 (MQ=25)
                                                  aTGCTTTTTCTTCCAAATT‑TGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGAT‑GAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGAtagta                                                                                            >  1:184727/1‑141 (MQ=35)
                                                        tttCTTCCAAATT‑TGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGAT‑GAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGccgtt                                                                                      <  1:544293/141‑4 (MQ=25)
                                                                        aTACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGAT‑GAACGTTTACACCACGTTCCCTTAATATTTCAGATGTCTCACGATAACTCAATGTATATCTTAGATAGTAGCCAGCGGCTACAGTGATaaa                                                                       >  1:26528/1‑140 (MQ=255)
                                                                              aaTTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGAT‑GAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCtt                                                                 <  1:883063/141‑1 (MQ=32)
                                                                                 tGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGAT‑GAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCCCGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGtt                                                              <  2:288636/141‑1 (MQ=17)
                                                                                    ggCATATTCTTGAACCCAACGGTAGACCGTTGAATGAT‑GAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTaaa                                                           >  2:256505/1‑141 (MQ=32)
                                                                                     gCATATTCTTGAACCCAACGGTAGACCGTTGAATGAT‑GAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAAt                                                          >  2:880897/1‑141 (MQ=32)
                                                                                       atatTCTTGAACCCAACGGTAGACCGTTGAATGAT‑GAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGCGATAACATCCTTGTTAAATTg                                                        <  1:286313/141‑1 (MQ=17)
                                                                                        tatTCTTGAACCCAACGGTAGACCGTTGAATGAT‑GAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGt                                                       <  2:27748/141‑1 (MQ=32)
                                                                                              tGAACCCAACGGTAGACCGTTGAATGAT‑GAACGTTTACACCACGTTCCCTTAATCTTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTatat                                                 >  1:153761/1‑141 (MQ=17)
                                                                                               tgactcAATGGTAGAACCTAGAAATAT‑GACCGTTCACACCACGTTCTCTTAACATTTCAGATATATCACGATAGATAAGTGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATc                                                <  1:132173/136‑1 (MQ=11)
                                                                                                 aCCCAACGGTAGACCGTTGAATGAT‑GAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTg                                              >  1:819449/1‑141 (MQ=32)
                                                                                                           aGACCGTTGAATGAA‑GAACGTTTACACCACGTTCCCTTAATATTTCAGATATAACACGGTAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAAGTGTTTATATCTGAAATAGTTCa                                    <  1:659888/141‑1 (MQ=255)
                                                                                                              tcGTTGAAGGAT‑GAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATAc                                 <  2:701723/140‑1 (MQ=12)
                                                                                                                     atgat‑tAACGTGTACACCAGGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGAc                          <  2:410920/141‑1 (MQ=12)
                                                                                                                            aaCTTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCttt                    <  2:80749/141‑1 (MQ=17)
                                                                                                                                   acacCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTaa             >  1:858728/1‑141 (MQ=32)
                                                                                                                                              |                                                                                                                                            
TTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATT‑TGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGAT‑GAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTAT  >  CP000730/36304‑36584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑