Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 111,330 C→A 6.5% Q134K (CAA→AAA)  USA300HOU_0110 → possible staphylococcal tandem lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730111,3300CA 6.5% 481.9 / 5.4 162Q134K (CAA→AAA) USA300HOU_0110possible staphylococcal tandem lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (77/71);  new base A (2/12);  total (79/83)
Fisher's exact test for biased strand distribution p-value = 9.79e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 168 total aligned reads displayed.

AAATGGCAATTCAAAATAAAGGAGAAGCTCTAAAAATAAAAGGCATGCTTTTGAAGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAA  >  CP000730/111190‑111470
                                                                                                                                             |                                                                                                                                             
aaaTGGCAATTCAAAACAAAGGAGAAGCTCTAAAAATAAAAGGCATGCTTTTGAAGATAAATAGAAATACAAGAAGTGCAAAAGGATTTTACTAGACAAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATc                                                                                                                                               >  1:467244/1‑141 (MQ=255)
 aaTGGCAATTCAAAATAAAGGAGAAGCTCTAAAAATAAAAGGCATGCTTTTGAAGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCa                                                                                                                                             >  2:368219/1‑141 (MQ=255)
  aTGGCAATTCAAAATTAAGGAGAAGCTTTCAAAATAAAAGGCATGCAATTGAAGTTAGATAGAAATAAAAGAAGTGCAAAAGGATCTTACTTTACTAATGAAATAAAGACGGAGAAATACGAAGTAGATCAGAATAATCaa                                                                                                                                            >  2:424399/1‑141 (MQ=255)
    ggCAATTCAAAATAAAGGAGAAGCTCTAAAAATAAAAGGCATGCTTTTGAAGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATaaaaaaaa                                                                                                                                          <  2:284257/141‑1 (MQ=255)
      cAATTCAAAATAAAGGAGAAGCTCTAAAAATAAAAGGCATGCTTTTGAAGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCaaaaaa                                                                                                                                        >  1:521534/1‑141 (MQ=255)
              aaTAAAGGAGAAGCTCTAAAAATAAAAGGCATGCTTTTGAAGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAGTATCCa                                                                                                                                >  1:309213/1‑141 (MQ=255)
              aaTAAAGGAGAAGCTCTAAAAATAAAAGGCATGCTTTTGAAGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCa                                                                                                                                >  2:506883/1‑141 (MQ=255)
               aTAAAGGAGAAGCTCTAAAAATAAAAGGCATGCTTTTGAAGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAg                                                                                                                               <  1:385779/141‑1 (MQ=255)
                 aaaGGAGAAGCTCTAAAAATAAAAGGCATGCTTTTGAAGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGCAATAAATACGGACAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGtt                                                                                                                             <  1:366228/141‑1 (MQ=255)
                 aaaGGAGAAGCTATAAAAAAAAAAGGCATGCTTTTGAAGAAAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGAAAAATCAAAAAAAATATCCAGtt                                                                                                                             <  2:761644/141‑1 (MQ=255)
                   aGGAGAAGCTCAAAAAAAAAAAGGAATTCGTTTGAAGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAAAGAAATAAAGACGGAGAAATTCGATGTAGTTGAAAAAAAAAAAAAAAAATATCCAGTTaa                                                                                                                           <  2:855818/141‑1 (MQ=255)
                      agaAGCTCTAAAAATAAAAGGCATGCTTTTGAAGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAAt                                                                                                                        >  2:928451/1‑141 (MQ=255)
                      agaAGCTCTAAAAATAAAAGGCATGCTTTTGAAGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAAt                                                                                                                        >  2:554136/1‑141 (MQ=255)
                      agaAGCTCTAAAAATAAAAGGCATGCTTTTGAAGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAAt                                                                                                                        >  2:176736/1‑141 (MQ=255)
                          gCTCTAAAAATAAAAGGCATGCTTTTGAAGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGAtt                                                                                                                    <  1:657874/141‑1 (MQ=255)
                           ctctAAAAATAAAAGGCATGCTTTTGAAGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATta                                                                                                                   >  2:300397/1‑141 (MQ=255)
                              tAAAAATAAAAGGCATGCTTTTGAAGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATtaata                                                                                                                >  1:53097/1‑141 (MQ=255)
                              tAAAAATAAAAGGCATGCTTTTGAAGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAAAATCCAATACAAATTATtaata                                                                                                                >  2:888703/1‑141 (MQ=255)
                                 aaaTAAAGGGCAGGCTTTTGAAGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATtaataata                                                                                                             <  1:25990/141‑1 (MQ=255)
                                 aaaTAAAAGGCATGCTTTGGAAGATAGATAGAAATAAAAGAAGTGCAAAAGGATTCTACTATACTAATGAAATAAAGAAGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATtaataata                                                                                                             <  2:574289/141‑1 (MQ=255)
                                  aaTAAAAGGCATGCTTTTGAAGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATtaataataa                                                                                                            <  1:592315/141‑1 (MQ=255)
                                  aaTAAAACGCAATATTTATAATATAGATATAAAAACAAGAAGTGCAAAAGCATTTTACCATACTAATAAAATAAAGACGGAGAGATGCGAAGTAGCTAAAAAAAAAAAAAAAAAATATCCAGTTAAAATGATtaataataa                                                                                                            <  2:448844/141‑1 (MQ=255)
                                    tAAAAGGCATGCTTTTGAAGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAAt                                                                                                          >  2:874780/1‑141 (MQ=255)
                                            aagattttGAAGATAGAGAGAAAGACAAGAAGTGCAAAAGGATTTTACTAAACTAATGAAATAAAGACGGAGAAACACGTAGTAGCTAAAAAAAAAAAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCt                                                                                                  <  2:342920/137‑1 (MQ=255)
                                            aTGCTTTGTAAGATAGATACAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCt                                                                                                  <  2:194378/141‑1 (MQ=255)
                                                ttttGAAGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTg                                                                                              >  1:631192/1‑141 (MQ=255)
                                                    gAAGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAAGCAAAAAAAATATCCAGGTAAAATGATTAATAATAAATTCATTTCTACTGAGGa                                                                                          <  2:82514/141‑1 (MQ=255)
                                                     aaGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGGAAGAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGaa                                                                                         >  1:735397/1‑141 (MQ=255)
                                                     aaGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGaa                                                                                         >  1:138263/1‑141 (MQ=255)
                                                        aaagaaaaaaaaACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACAGAGGAAGtt                                                                                      <  2:606835/133‑1 (MQ=255)
                                                         tagatagaAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTa                                                                                     >  1:454258/1‑141 (MQ=255)
                                                         tagatagaAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAAACGGAGAAATAAGAAGTAGCTCAGGATAATCAAAAAAAATCTCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGCGGCAGTTa                                                                                     >  1:25568/1‑141 (MQ=255)
                                                         tagatagaAATACAAGAAGTGCAAAAGGATGTTACTAGAAAAAAAGCTGATGAATTGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTa                                                                                     <  1:922947/141‑1 (MQ=255)
                                                          agatagaAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTaa                                                                                    <  1:708037/141‑1 (MQ=255)
                                                          agatacaACAACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTaa                                                                                    <  1:169366/141‑1 (MQ=255)
                                                           acaaaaaaTAAAAGAAGGACAAAAGGATTTTAATATAATAAAGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTaaa                                                                                   <  2:25568/136‑1 (MQ=255)
                                                            atagaAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAaag                                                                                  <  1:92015/141‑1 (MQ=255)
                                                             aagaAAAACAAGAAGTGAAAAAGGATGTTACTATACAAAAAAAATAAAGACGGAGAAATACGAAGTAGCTAAAAAAAAAAAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAaaga                                                                                 <  2:396771/140‑1 (MQ=255)
                                                              agaAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAaagaa                                                                                >  2:896797/1‑141 (MQ=255)
                                                               aaaaTACAAGAAGTGCAAAAGGATAGTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAaagaag                                                                               <  2:543731/140‑1 (MQ=255)
                                                                aaaTACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAATAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAagaaga                                                                              >  2:15191/1‑141 (MQ=255)
                                                                    aCAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTAATTTCTACTGAGGAAGTTAAAGAAGaaaaa                                                                          >  2:505230/1‑140 (MQ=255)
                                                                    aCAAGAAGTGCAAAAGGATTTGACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAAc                                                                          <  1:769950/141‑1 (MQ=255)
                                                                     cAAGAAGTGTAAAAGAATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTAAAAATAAAAAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACa                                                                         <  2:777688/141‑1 (MQ=255)
                                                                     cAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCCTTTCTACTGAGGAAGTgaaaacagaaaaca                                                                         >  2:600152/1‑141 (MQ=255)
                                                                     aaagaagTGCAAAAGGATTATACTATACTAATGACATAAAGACGGAGAAATACGAAGTAGCTAAAAAAAAAAAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACa                                                                         <  2:821627/140‑1 (MQ=255)
                                                                           gTGCAAAAGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATaaaaa                                                                   >  2:364500/1‑141 (MQ=255)
                                                                             gCAAAAGGATTTTACTATACTAATGAAATACAGACGGAGAAATACGAAGTAGCTCAGGATAATCAGAAAAAATATCCAGTTAAAATACTTAATAATAAATTCATATCTCCTGAGACAGTTACAGACACACACATaaatata                                                                 >  2:883703/1‑137 (MQ=255)
                                                                             gCAAAAGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATaaaaaaa                                                                 >  2:385740/1‑141 (MQ=255)
                                                                                 aaGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGaaa                                                             >  1:616470/1‑141 (MQ=255)
                                                                                    gATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCg                                                          <  2:555500/141‑1 (MQ=255)
                                                                                           ctatactaATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTAATGGGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACtt                                                   <  1:648026/141‑1 (MQ=255)
                                                                                           atatactaATGAAATAAAGACTGAGAAATACGAAGTAGCTCAGGATAATCAAAAATTATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACtt                                                   <  2:467244/140‑1 (MQ=255)
                                                                                            tatactaATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACttt                                                  <  1:318658/141‑1 (MQ=255)
                                                                                            tatactaATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACttt                                                  <  2:20414/141‑1 (MQ=255)
                                                                                             atactaATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAACAAGAAAACATAAAAAAAGAAATCGAAAACTTTa                                                 >  1:734338/1‑141 (MQ=255)
                                                                                               accaataaaataaAAACGGAGGAATGCGAAGTAGCTAAAAAAAAACAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGt                                              <  2:79902/138‑1 (MQ=37)
                                                                                               aataataaAAAAAAGACGGAGAAACACGAATTAGCTAAAAAAAAAAAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAg                                               <  2:22742/139‑1 (MQ=37)
                                                                                                gcaaTGAAATAAGAACAGACAAATACTAATTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATTATTAATAATAAATTCATTTCTACTGAGGAAGTTCAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGt                                              <  2:221880/139‑1 (MQ=37)
                                                                                                ctaATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAGGAAAAAAATGACTACTCAGGAAGTTAAAGAAGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGt                                              >  1:448616/1‑141 (MQ=37)
                                                                                                  aaTGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGttt                                            <  1:313748/141‑1 (MQ=255)
                                                                                                       aaTAAAGACGGAGACATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTACAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTga                                       >  1:494144/1‑140 (MQ=255)
                                                                                                       aaTAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTCAGTTATTTgc                                       >  2:403061/1‑141 (MQ=255)
                                                                                                       aaTAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTgc                                       <  1:579906/141‑1 (MQ=255)
                                                                                                       aaTAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTgc                                       <  1:43089/141‑1 (MQ=255)
                                                                                                        aTAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTgcg                                      <  1:506883/141‑1 (MQ=255)
                                                                                                         aaaaTACAGAAAAATACGAAATAACACAGAAAAAAAAAAAAAAAAAACCAGTTAAAAAGATAAAAAATAAATTCATTTATACTGAGGAAGTTAAAGAAAAAAAAAAAAAAAAAGAAATCGAAAACTTTAAGTTTTTTgcgc                                     <  2:617145/140‑1 (MQ=19)
                                                                                                         aaaaGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTgcgc                                     <  1:543754/140‑1 (MQ=255)
                                                                                                          aaaGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTCAAACGAGTAATAATATATTCATTTCTACTGAGGAAGTTAGAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCa                                    >  2:383471/1‑141 (MQ=38)
                                                                                                          aaaGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTTCGCa                                    >  2:136711/1‑141 (MQ=255)
                                                                                                          aaaGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCa                                    >  2:849179/1‑141 (MQ=255)
                                                                                                          aaaGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCa                                    >  1:376381/1‑141 (MQ=255)
                                                                                                          aaaGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCa                                    >  2:484207/1‑141 (MQ=255)
                                                                                                           aaGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCaa                                   <  1:132697/141‑1 (MQ=255)
                                                                                                           aaGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCaa                                   >  1:491833/1‑141 (MQ=255)
                                                                                                           aaGACGGAGAAATACGAAGTAGCTCAGAATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCaa                                   <  2:660602/141‑1 (MQ=255)
                                                                                                            aGACGGAGAAATGCGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAAGATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAat                                  >  1:667357/1‑141 (MQ=255)
                                                                                                            aGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAat                                  >  1:136187/1‑141 (MQ=255)
                                                                                                               aCGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAataa                                >  2:197183/1‑140 (MQ=255)
                                                                                                                cGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAAtata                               <  1:31080/141‑1 (MQ=255)
                                                                                                                cGGAGAAATACGAAGTAGCTCAGGATAATAAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAAtata                               >  1:654569/1‑141 (MQ=255)
                                                                                                                  gagaAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGc                             <  1:334879/141‑1 (MQ=255)
                                                                                                                  gagaAATACGAAGTAGATCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGc                             <  2:124929/141‑1 (MQ=255)
                                                                                                                     aaaTACGAAGTAGCTCAGGATAATCAAAACCCATATCCAGATAAAACGATTAATATTCAATTAATATATACAGAGACAGTAGTAGTACCAGATATAATAAAATTAATCTATAATTTTAAGTTTTTTTCGCAATATAGCAAt                          >  2:81933/1‑141 (MQ=255)
                                                                                                                     aaaTACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAAt                          >  2:449599/1‑141 (MQ=255)
                                                                                                                     aaaTACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAAt                          >  1:849101/1‑141 (MQ=255)
                                                                                                                     aaaTACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAAt                          <  1:211849/141‑1 (MQ=255)
                                                                                                                       attcGAAGTGGTTAAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAAttt                        <  2:120119/138‑1 (MQ=255)
                                                                                                                       atgtgaaggtgCTTAGGATAATCAAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAAtt                         <  2:410226/131‑1 (MQ=37)
                                                                                                                       aTACGAAGTAGCTCAGGATAATCAAAAAGAATATCCAGTTAAAATGATTAATAAGAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAAttt                        >  1:608741/1‑141 (MQ=255)
                                                                                                                         aCGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTa                      <  1:887741/141‑1 (MQ=255)
                                                                                                                            aaGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAg                   <  2:738128/141‑1 (MQ=255)
                                                                                                                            aaGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAg                   <  1:561644/141‑1 (MQ=255)
                                                                                                                                   tCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAAt            <  1:364500/141‑1 (MQ=255)
                                                                                                                                      ggATAATCAAAAACAATATCCAGTTAAAATGAATAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGaa         <  2:680331/141‑1 (MQ=255)
                                                                                                                                         aaaTCAAAAAAAATATCTCGTTAAAATGATTAATAATATAATCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAATACTTTAAGTTTTTTGCGCAATATAGCAATCTTAAAGATTTAATGAATta      <  1:612610/140‑1 (MQ=37)
                                                                                                                                          aaTCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATtat     <  1:502476/141‑1 (MQ=255)
                                                                                                                                           aTCAAAAAAAATATCCCGTTAAAATAATTAATAATAAATTCATTTCTACTGCGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGGAATATAGCAATTTTACAGATTTAATGAATtata    >  2:321558/1‑141 (MQ=37)
                                                                                                                                            tCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTc                                                                                                        <  1:824072/40‑1 (MQ=255)
                                                                                                                                            tCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATaa   >  1:222652/1‑141 (MQ=255)
                                                                                                                                             |                                                                                                                                             
AAATGGCAATTCAAAATAAAGGAGAAGCTCTAAAAATAAAAGGCATGCTTTTGAAGATAGATAGAAATACAAGAAGTGCAAAAGGATTTTACTATACTAATGAAATAAAGACGGAGAAATACGAAGTAGCTCAGGATAATCAAAAAAAATATCCAGTTAAAATGATTAATAATAAATTCATTTCTACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAA  >  CP000730/111190‑111470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: