Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 35,876 C→T 47.3% intergenic (+107/+1) rlmH → / ← tpn hypothetical protein/IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073035,8760CT47.3% 101.9 / 269.4 231intergenic (+107/+1)rlmH/tpnhypothetical protein/IS431mec transposase
Reads supporting (aligned to +/- strand):  ref base C (58/63);  new base T (50/58);  total (109/122)
Fisher's exact test for biased strand distribution p-value = 8.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.58e-02

Only 100 of 237 total aligned reads displayed.

AGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACC  >  CP000730/35736‑36016
                                                                                                                                            |                                                                                                                                            
aGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGc                                                                                                                                              >  1:559189/1‑141 (MQ=255)
    aTTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTaa                                                                                                                                          >  1:54521/1‑141 (MQ=255)
      tCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACt                                                                                                                                        >  1:251862/1‑141 (MQ=255)
          tataAAGTTTTTGGCGAAAAATGATTACCTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTCCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCt                                                                                                                                    <  1:654757/141‑1 (MQ=255)
          aaaaaaGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCt                                                                                                                                    <  1:919143/138‑1 (MQ=255)
                   gttGGCGAACCATGACTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCatgatg                                                                                                                           >  2:914591/2‑141 (MQ=255)
                        cGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGATCTGTTGCAAAGTGAAAAAATATAATACTTAAACAATGATTCATGTCAGTGTTGGTTTAACTTGCTAGCATGATGCTAAt                                                                                                                      >  1:680910/1‑141 (MQ=25)
                             aaTGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGt                                                                                                                 >  1:157799/1‑141 (MQ=255)
                              aTGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTg                                                                                                                >  2:630339/1‑141 (MQ=255)
                              aTGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTg                                                                                                                <  1:356985/141‑1 (MQ=255)
                                gATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGc                                                                                                              >  2:893729/1‑141 (MQ=255)
                                gATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGc                                                                                                              <  1:329884/141‑1 (MQ=255)
                                   tACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATg                                                                                                           >  2:159782/1‑141 (MQ=255)
                                        gAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGaa                                                                                                      <  1:127694/141‑1 (MQ=255)
                                           cAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAAt                                                                                                   <  1:321502/141‑1 (MQ=255)
                                             agagGTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATcc                                                                                                 <  2:890240/141‑1 (MQ=255)
                                             agagCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATcc                                                                                                 >  2:474122/1‑141 (MQ=255)
                                             aaagataaaataaaaTTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATcc                                                                                                 <  2:540250/136‑1 (MQ=255)
                                              gagCTAAAATAAAATTGTGCTCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACGAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCg                                                                                                >  2:651850/1‑141 (MQ=255)
                                               agCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGt                                                                                               >  1:457827/1‑141 (MQ=255)
                                               agCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTACTGTTGCAAAGTAAAAAAATATGGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGCTGCTAATTTCGTGCAATGGAGAAAACCCga                                                                                               >  2:777517/1‑140 (MQ=18)
                                                  taaaataaaaTTGTGACCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGa                                                                                            <  1:814373/141‑1 (MQ=255)
                                                       caaaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAag                                                                                     >  2:351325/14‑141 (MQ=11)
                                                       caaaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAag                                                                                     <  1:875796/128‑1 (MQ=11)
                                                       caaaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAag                                                                                     <  2:612587/128‑1 (MQ=11)
                                                       caaaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAag                                                                                     <  1:279027/128‑1 (MQ=11)
                                                        aaaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAACTATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAaga                                                                                    <  2:839020/129‑1 (MQ=11)
                                                          aagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAgaga                                                                                  <  1:727922/131‑1 (MQ=11)
                                                            gagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTATTATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAGTCCGTAGATCTGGAGAGAcc                                                                                <  1:594572/133‑1 (MQ=11)
                                                            gagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGGTCTGAAGAGAcc                                                                                >  2:743076/9‑141 (MQ=11)
                                                            gagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                                                                                >  1:881506/9‑141 (MQ=11)
                                                             tgtgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACCTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                                                                 <  1:220618/141‑1 (MQ=255)
                                                             tgtgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTAGCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                                                                 <  1:198453/141‑1 (MQ=255)
                                                             tgtgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAAGTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTGCGTGGCATGGCGAAAATCCGTTGATCGGGAGAGAc                                                                                 <  2:437310/141‑1 (MQ=14)
                                                             agtattttaataaAAATGACGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCt                                                                               <  1:528900/134‑1 (MQ=11)
                                                              ttgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                                                                                <  1:506877/140‑1 (MQ=255)
                                                              gtgATTCAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCAGGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                                                                                <  2:767311/141‑1 (MQ=255)
                                                              gtgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                                                                                >  1:539373/1‑141 (MQ=255)
                                                              gtgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                                                                                >  2:722151/1‑141 (MQ=255)
                                                              gtgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                                                                                <  2:591475/141‑1 (MQ=255)
                                                              gtgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                                                                                <  1:136210/141‑1 (MQ=255)
                                                               tgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCt                                                                               <  1:858566/141‑1 (MQ=255)
                                                               tattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                                                                             >  2:671629/6‑141 (MQ=11)
                                                                attttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTTATTTCGTGGCATGGCGAAAATCCGTAGATCTTAAGAGACCTGCg                                                                            >  1:617291/5‑141 (MQ=11)
                                                                attttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg                                                                            <  1:432520/137‑1 (MQ=11)
                                                                attttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg                                                                            <  1:402251/137‑1 (MQ=11)
                                                                attttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGGCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGCCCTGCg                                                                            >  2:455095/5‑141 (MQ=11)
                                                                  tttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGt                                                                          <  1:789501/139‑1 (MQ=11)
                                                                  ttgaataaAAATGAGGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATACATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGTCCTgatcg                                                                          <  2:244934/138‑5 (MQ=11)
                                                                   ttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGTAGAGACCTGCGGtt                                                                         >  2:326707/2‑141 (MQ=11)
                                                                    taataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTc                                                                        <  2:30591/141‑1 (MQ=11)
                                                                    taataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCGAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTc                                                                        <  2:45145/141‑1 (MQ=11)
                                                                     aataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAAGCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCt                                                                       <  2:75171/141‑1 (MQ=11)
                                                                       taaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCttt                                                                     <  2:667361/141‑1 (MQ=11)
                                                                       aataaatggcggggtCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATGTATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAACTCCGTAGATCTGAAGAGACCTACGGTTCtt                                                                      >  2:693870/15‑141 (MQ=1)
                                                                        aaaaaTAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCtt                                                                      >  1:777612/1‑141 (MQ=37)
                                                                        aaaaaTAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGAATGATGCTAATTTCGTGGCAGGGCGAAAATCCGTAGATGTGAAGGGACCTGCGGTCCtt                                                                      >  1:601916/1‑141 (MQ=11)
                                                                           aaatgatgggtCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGGAGAGACCTGCGGTTCtttttt                                                                  >  1:534462/11‑140 (MQ=1)
                                                                           aaTAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCttttt                                                                   <  2:533254/141‑1 (MQ=34)
                                                                            aTAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTta                                                                  <  1:886752/141‑1 (MQ=33)
                                                                              aGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAGTTTCGTGGCACGGCGAAAATCCGCAGATATGGAGAGACCTGCgggtgttatgata                                                                >  2:497367/1‑130 (MQ=11)
                                                                              aGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTtata                                                                >  1:79804/1‑141 (MQ=32)
                                                                              aGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCAGGATGCTAATTTCGTGGCATGGAGCAAATGCGTAGATCTGAAGAGACCTGCGGTTCTTTTtata                                                                >  1:637881/1‑141 (MQ=255)
                                                                                      ttgctGCAAAGTAAAAAATATGGCTACTCACTAATATATCATGTCAGTGTTGGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATTCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTa                                                       <  2:373134/137‑1 (MQ=1)
                                                                                       tgctGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTa                                                       <  2:559605/138‑1 (MQ=1)
                                                                                         ttccAAAGTAAAAAAATCTAGCTAACCACTAATTTATCATGTCATTGTGCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGGGCGTaaa                                                     <  2:414876/138‑1 (MQ=1)
                                                                                                                aatcACTAAGATATTGTGTCAGTTTTCGTTTAACTTGCTAGCATGATGCTAATTACGTGGCAAGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTGTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTa                              <  1:28744/138‑1 (MQ=1)
                                                                                                                  ccccTAATTTATAATGTCTGTTTTCGCCTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCTGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTAtt                            <  2:113730/138‑1 (MQ=1)
                                                                                                                                   cAGTGTTCGTTTAACTTTCTAGCATTATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATAc           <  1:302511/141‑1 (MQ=2)
                                                                                                                                    aGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGGAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACt          >  2:604880/1‑141 (MQ=11)
                                                                                                                                    aGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACt          >  2:920599/1‑141 (MQ=255)
                                                                                                                                    aGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACt          >  2:83426/1‑141 (MQ=255)
                                                                                                                                    aGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACt          >  1:18853/1‑141 (MQ=255)
                                                                                                                                    aGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTACATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACt          >  2:144689/1‑141 (MQ=9)
                                                                                                                                    aGTGTTCGTTTAACTTGCTAGAATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATATAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACt          <  1:696342/141‑1 (MQ=11)
                                                                                                                                    aGTGTTCGCTTAACTTGCTCGCATGATGCTATTTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACt          <  2:19136/141‑1 (MQ=9)
                                                                                                                                     gtgtTCGTTTAACTTGGTAGCTTGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACtt         >  1:483163/1‑141 (MQ=255)
                                                                                                                                     gtgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATATGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACtt         >  2:63172/1‑141 (MQ=11)
                                                                                                                                     gtgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCAGGGGGACAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTGAAGTATTCTTTGCCGTATTGATACtt         >  1:366618/1‑141 (MQ=11)
                                                                                                                                     gtgtTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTTATACtt         >  1:6968/1‑141 (MQ=17)
                                                                                                                                     gtgtTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACtt         >  1:833100/1‑141 (MQ=17)
                                                                                                                                      tgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACttt        >  1:544516/1‑141 (MQ=255)
                                                                                                                                      tgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACttt        >  1:820289/1‑141 (MQ=17)
                                                                                                                                       tttCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTg       <  1:905979/140‑1 (MQ=11)
                                                                                                                                       gtTCGTTTAACTTGCTAGCATGATTCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTg       <  2:253869/141‑1 (MQ=11)
                                                                                                                                       gtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTg       >  2:550704/1‑141 (MQ=255)
                                                                                                                                       gtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTg       >  2:221291/1‑141 (MQ=255)
                                                                                                                                       gtTCGTTTAACTTGCTAGCATGATGCGAATTTCGTGGCATGGGGAAAATCCGTAGATCTCAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAATTATTCTTTGTCGTATTGATACTTTg       <  2:218164/141‑1 (MQ=11)
                                                                                                                                       gtTCGTCTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTCGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTCTTACAGCCTTCTTTGCTGTATTGATACttag       >  2:48978/1‑139 (MQ=11)
                                                                                                                                        ttCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGa      <  2:477134/141‑1 (MQ=32)
                                                                                                                                        ttCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGa      <  2:255414/141‑1 (MQ=17)
                                                                                                                                        ttCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGa      <  1:73963/141‑1 (MQ=17)
                                                                                                                                          cGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATCTAGAGCGTAAATAAATTCAATACCTTTTAAAGTAGTCTTTGCTGTATTGATACTTTGAtt    >  2:787865/1‑140 (MQ=12)
                                                                                                                                          cGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATa    >  1:229918/1‑141 (MQ=17)
                                                                                                                                          cGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATa    >  1:50235/1‑141 (MQ=17)
                                                                                                                                           gCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAc   <  2:883029/141‑1 (MQ=17)
                                                                                                                                           gCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAc   <  2:638881/141‑1 (MQ=17)
                                                                                                                                           gCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAc   <  1:852350/141‑1 (MQ=17)
                                                                                                                                            cTTAACTTGCTAGGATGATGCTAATTTCGTGGCATGGCCAAAATCCGTAGATCTGAACAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAcc  <  2:320964/141‑1 (MQ=12)
                                                                                                                                            cTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAcc  <  1:179791/141‑1 (MQ=17)
                                                                                                                                            |                                                                                                                                            
AGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACC  >  CP000730/35736‑36016

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: