Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,433,870 A→G 10.7% D287G (GAC→GGC)  dapA → dihydrodipicolinate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,433,8700AG10.7% 133.4 / 6.0 55D287G (GAC→GGC) dapAdihydrodipicolinate synthase
Reads supporting (aligned to +/- strand):  ref base A (28/20);  new base G (3/3);  total (32/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTATCAGCTTTATCAGTTGATATTAACCCAATTCCTATTAAAGCTCTAACAAGTTATTTAGGATTTGGAAATTATGAATTACGTCTACCATTGGTTAGCCTAGAAGATACAGATACTAAAGTGCTTCGTGAAACATATGACACATTTAAAGCGGGTGAAAATGAGTGAAAATATTACTAATTGGCTATGGCGCAATGAATCAACGCGTTGCTAGATTAGCAGAAGAAAAAGGACATGAAATCGTTGGGGTCATTGAAAATACACCGAAAGCAACAACGC  >  CP000730/1433730‑1434009
                                                                                                                                            |                                                                                                                                           
gTTATCAGCTTTATCAGTTGATATTAACCCAATTCCTATTAAAGCTCTAACAAGTTATTTAGGATTTGGAAATTATGAATTACGTCTACCATTGGTTAGCCTAGAAGATACAGATACTAAAGTGCTTCGTGAACCACATGa                                                                                                                                             >  2:510273/1‑141 (MQ=255)
gTTATCAGCTTTATCAGTTGATATTAACCCAATTCCTATTAAAGCTCTAACAAGTTATTTAGGATTTGGAAATTATGAATTACGTCTACCATTGGTTAGCCTAGAAGATACAGATACTAAAGTGCTTCGTGAAACATATGa                                                                                                                                             >  1:396079/1‑141 (MQ=255)
 ttATCAGCTTTATCAGTTGATATTAACCCAATTCCTATTAAAGCTCTAACAAGTTATTTAGGATTTGGAAATTATGAATTACGTCTACCATTGGTTAGCCTAGAAGATACAGATACTAAAGTGCTTCGTGAAACATATGac                                                                                                                                            <  2:418646/141‑1 (MQ=255)
   aTCAGCTTTATCAGTTGATATTAACCCAATTCCTATTAAAGCTCTAACAAGTTATTTAGGATTTGGAAATTATGAATTACGTCTACCATTGGTTAGCCTAGAAGATACAGATACTAAAGTGCTTCGTGAAACATATGacac                                                                                                                                          <  2:380649/141‑1 (MQ=255)
   aTCAGCTTTATCAGTTGATAATAACCCACTGCCTATTAAATCTCTAACAAGTTATTTAGGATTTGGAAATTATGAATCACGTCTACCATCGGTTAGCCTAGAAGATACAGATACTAAAGTGCTTCGTGAAACATATGacac                                                                                                                                          <  2:901913/141‑1 (MQ=255)
    tCAGCTTTATCAGTTGATATTAACCCAATTCCTATTAAAGCTCTAACAAGTTATTTAGGATTTGGAAATTATGAATTACGTCTACCATTGGTTAGCCTAGAAGATACAGATACTAAAGTGCTTCGTGAAACATATGAcaca                                                                                                                                         <  1:25000/141‑1 (MQ=255)
    tCAGCTTTATCAGTTGATATTAACCCAATTCCTATTAAAGCTCTAACAAGTTATTTAGGATTTGGAAATTATGAATTACGTCTACCATTGGTTAGCCTAGAAGATACAGATACTAAAGTGCTTCGTGAAACATATGAcaca                                                                                                                                         <  1:864615/141‑1 (MQ=255)
      aGCTTTATCAGTTGATATTAACCCAATTCCTATTAAAGCTCTAACAAGTTATTTAGGATTTGGAAATTATGAATTACGTCTACCATTGGTTAGCCTAGAAGATACAGATACTAAAGTGCTTCGTGAAACATATGACACAtt                                                                                                                                       <  2:924406/141‑1 (MQ=255)
          ttATCAGTTGATATTAACCCAATTCCTATTAACGATCTAACAAGTTATCTAGGATTTGGAAATTATGAATTACGTCTACCATTGGTTAGCCTAGAAGATACAGATACTAAAGTGCTTCGTGAAACATATGACACATTTaaa                                                                                                                                   >  1:222354/1‑141 (MQ=255)
                      atTAACCCAATTCCTATTAAAGCTCTAACAAGTTATTTAGGATTTGGAAATTATGAATTACGTCTACCATTGGTTAGCCTAGAAGATACAGATACTAAAGTGCTTCGTGAAACATATGACACATTTAAAGCGGGTGAAAAt                                                                                                                       >  1:481617/1‑141 (MQ=255)
                      atTAACCCAATTCCTATTAAAGCTCTAACAAGTTATTTAGGATTTGGAAATTATGAATTACGTCTACCATTGGTTAGCCTAGAAGATACAGATACTAAAGTGCTTCGTGAAACATATGACACATTTAAAGCGGGTGAAAAt                                                                                                                       >  1:777640/1‑141 (MQ=255)
                      atTAACCCAATTCCTATTAAAGCTCTAACAAGTTATTTAGGAGTTGGAAATTATGAATTACGTCTACCATTGGTTAGCCTAGAAGATACAGATACTAAAGTGCTTCGTGAAACATATGACACATTTAAAGCGGGTGAAAAt                                                                                                                       >  2:3905/1‑141 (MQ=255)
                      atTAACCCAATTCCTATTAAAGCTCTAACAAGTTAATTCGGATTTGGAAATTATGAATTACGTCTACCATTGGTTAGCCTAGAAGATACAGATACTAAAGTGCTTCGTGAAACATATGACACATTTAAAGCGGGTGAAAAt                                                                                                                       >  1:889995/1‑141 (MQ=255)
                         aaCCCAATTCCTATTAAAGCTCTAACAAGTTATTTAGGATTTGGAAATTATGAATTACGTCTACCATTGGTTAGCCTAGAAGATACAGATACTAAAGTGCTTCGTGAAACATATGACACATTTAAAGCGGGTGAAAATGAg                                                                                                                    >  1:746108/1‑141 (MQ=255)
                                      ttAAAGCTCTAACAAGTTATTTAGGATTTGGAAATTATGAATTACGTCTACCATTGGTTAGCCTAGAAGATACAGATACTAAAGTGCTTCGTGAAACATATGGCACATTTAAAGCGGGTGAAAATGAGTGAAAATATTACt                                                                                                       >  1:702180/1‑141 (MQ=255)
                                      ttAAAGCTCTAACAAGTTATTTAGGATTTGGAAATTATGAATTACGTCTACCATTGGTTAGCCTAGAAGATACAGATACTAAAGTGCTTCGTGAAACATATGGCACATTTAAAGCGGGTGAAAATGAGTGAAAATATTACt                                                                                                       >  1:518453/1‑141 (MQ=255)
                                      ttAAAGCTCTAACAAGTTATTTAGGATTTGGAAATTATGAATTACGTCTACCATTGGTTAGCCTAGAAGATACAGATACTAAAGTGCTTCGTGAAACATATGGCACATTTAAAGCGGGTGAAAATGAGTGAAAATATTACt                                                                                                       >  2:440196/1‑141 (MQ=255)
                                           gcttgaaCAAGTTATTTAGGATTTGGAAATTATGAATTACGTCTACCATTGGTTAGCCTAGAAGATACAGATACTAAAGTGCTGCGTGAAACATATGACACATTTAAAGCGGGTGAAAATGAGTGAAAATATTACTAATTg                                                                                                  <  2:336987/136‑1 (MQ=255)
                                           gCTCTAACAAGTTAGTTAGGATTTGGAAATTATGAATTACGTCTACCATTGGTTAGCCTAGAAGATACAGATACTAAAGTGCTTCGTGAAACATATGACACATTTAAAGCGGGTGAAAATGAGTGAAAATATTACTAATTg                                                                                                  <  2:725460/141‑1 (MQ=255)
                                               tAACAAGTTATTTAGGATTTGGAAATTATGAATTACGTCTAGCTTTGGTTAGCCTAGAAGATACAGATACTAAAGTGCTTCGTGAAACATATGACACATTTAAAGCGGGTGAAAATGAGTGAGAATATTGCTAATTggttt                                                                                              >  2:620073/1‑138 (MQ=255)
                                               tAACAAGTTATTTAGGATTTGGAAATTATGAATTACGTCTACCATTGGTTAGCCTAGAAGATACAGATACTAAAGTGCTTCGTGAAACATATGACACATTTAAAGCGGGTGAAAATGAGTGAAAATATTACTAATTGGCTa                                                                                              >  2:360364/1‑141 (MQ=255)
                                               tAACAAGTTATACAGGATTTAGAAATCACGAAGCAGGTCTACCATTGGTTAGCCTAGAAGACACAGATACTAAAGTGCTTCGTGAAACATATAACACATTAAAATCGTGTGCAAATGAGTGAAAATATTACTAAtggcata                                                                                              >  2:160122/1‑135 (MQ=255)
                                                 aCAAGTTATTTAGGATTTGGAAATTATGAATTACGTCTACCATTGGTTAGCCTAGAAGATACAGATACTAAAGTGCTTCGTGAAACATATGACACATTTAAAGCGGGTGAAAATGAGTGTAAATATTACTAATTGGCTATg                                                                                            <  1:487864/141‑1 (MQ=255)
                                                 aCAAGTTATTTAGGATTTGGAAATTATGAATTACGTCTACCATTGGTTAGCCTAGAAGATACAGATACTAAAGTGCTTCGTGAAACATATGACACATTTAAAGCGGGTGAAAATGAGTAAAAATATTACTAATTGGCTATg                                                                                            >  1:116895/1‑141 (MQ=255)
                                                   aaGTTATTTAGGATTTGGAAATTATGAATTACGTCTACCATTGGTTAGCCTAGAAGATACAGATACTAAAGTGCTTCGTGAAACAGAGGCAACACCTAAAGCGGGGGAAAATGAGTGAAAATATTACGAAGTGGCtcgggc                                                                                          >  1:306980/1‑136 (MQ=255)
                                                              gATTTGGAAATTATGAATTACGTCTACCATTGGTTAGCCTAAAAGATACAGATACTAAAGTGCCTCGTGAAACAAATGACACATTTAAAGCGGGTGAAAATGAGTGAAAATATTACTAATTGGCTATGGCGCAATGAATCa                                                                               <  2:753124/141‑1 (MQ=255)
                                                                tttGGAAATTATGAATTACGTCTACCATTTGTTAGCCTAGAAGATACAGATACTAAAGTGCTTCGTGAAACATATGACACATTTAAAGCGGGTGAAAATGAGTGAAAATATTACTAATTGGCTATGGCGCAATGAATCAAc                                                                             <  2:136706/141‑1 (MQ=255)
                                                                tttGGAAATTATGAATTACGTCTACCATTGGTTAGCCTAGAAGATACAGATACTAAAGTGCTTCGTGAAACATATGACACATTTAAAGCGGGTGAAAATGAGTGAAAATATTACTAATTGGCTATGGCGCAATGAATCAAc                                                                             <  2:937976/141‑1 (MQ=255)
                                                                tttGGAAATTATGAAGTACGTCTACCATTGGTTAGCCTAGAAGATACAGATACTAAAGTGCTTCGTGAAACATATGACACATTTAAAGCGGGTGAAAACGAGTGAAAATATTACTAATTGGCTATGGCGCAATGAATCAAc                                                                             <  1:870624/141‑1 (MQ=255)
                                                                   ggAAATTATGAATTACGTCTACCAGTGGTTAGCCTAGAAGATACAGATACTAAAGTGCTTCGTGAAACATATGACACATTTAAAGCGGGTGAAAATGAGTGAAAATATTACTAATTGGCTATGGCGCAATGAATCAGcgcg                                                                          <  2:652504/141‑1 (MQ=255)
                                                                   ggAAAGCATGACTTACTGCTAACATTGGTCAGGCTAGAAGATACAGATACTAAAGTGCTTCGTGAAACATATGACACATTTAAAGCGGGTGAAAATGAGTGAAAATATTACTAATTGGCTATGGCGCAATGAATCAAcgcg                                                                          <  2:922536/141‑1 (MQ=255)
                                                                    gAAATTATGAATTACGTCTACCATTGGTTAGCCTAGAAGATACAGATACTAAAGTGCTTCGTGAAACATATGACACATTTAAAGCGGGGGAAAATGAGTGAAAATATTACTAATTGGCTATGGCGACATGAATCAACGCGt                                                                         >  2:604675/1‑141 (MQ=255)
                                                                       aTTATGAATTACGTCTACCATTGGTTAGCCTAGAAGATACAGATACTAAAGTGCTTCGTGAAACATATGACACATTTAAAGCGGGTGAAAATGAGTGAAAATATTACTAATTGGCTATGGCGCAATGAATCAACGCGTTGc                                                                      <  1:594889/141‑1 (MQ=255)
                                                                         tATGAATTACGTCTACCATTGGTTCGCCTAGAAGATACAGATACTAAAGTGCTTCGTGAAACATATGACACATTTAAAGCGGGTGAAAATGAGTGAAAATATTACTAATTGGCTATGGCGCAATGAATCAACGCGTTGCTa                                                                    >  1:687910/1‑141 (MQ=255)
                                                                             aaTTACGTCTACCATTGGTTAGCCTAGAAGATACAGATACTAAAGTGCTTCGTGAAACATATGACACATTTAAAGCGGGTGAAAATGAGTGAAAATATTACTAATTGGCTATGGCGCAATGAATCAACGCGTTGCTAGAtt                                                                >  1:495599/1‑141 (MQ=255)
                                                                                  cGTCTACCATTGGTTAGCCTAGAAGATACAGATACTAAAGTGCTTCGTGAAACATATGACACATTTAAAGCGGGTGAAAATGAGTGAAAATATTACTAATTGGCTATGGCGCAATGAATCAACGCGTTGCTAGATTAGCag                                                           <  1:33316/141‑1 (MQ=255)
                                                                                  cGTCTACCATTGGTTAGCCTAGAAGATACAGATACTAAAGTGCTTCGTGAAACATATGACACATTTAAAGCGGGTGAAAATGAGTGAAAATATTACTAATTGGCTATGGCGCAATGAATCAACGCGTTGCTAGATTAGCag                                                           <  1:59973/141‑1 (MQ=255)
                                                                                    tCTACCATTGGTTAGCCTAGAAGATACAGATACTAAAGTGCTTCGTGAAACATATGACACATTTAAAGCGGGTGAAAATGAGTGAAAATATTACTAATTGGCTATGGCGCAATGAATCAACGCGTTGCTAGATTAGCagaa                                                         <  1:189197/141‑1 (MQ=255)
                                                                                    gcTACCATTGGTTAGCCTAGAAGATACAGATACTAAAGTGCTTCGTGAAACATATGACACATTTAAAGCGGGTGAAAATGAGTGAAAATATTACTAATTGGCTATGGCGCAATGAATCAACGCGTTGCTAGATTAGCagaa                                                         <  1:481254/140‑1 (MQ=255)
                                                                                     tgaCCATTGGATAGCCTAGAAGATACAGAAACTAAAGGGCTTCGGGAAACATAAGACACATTTAAAGCGGGGGAAAATGAGGGAAAATATAACTAATTGGCTATGGCGCAATGAATCAACGCGTTGCTAGATTAGCagaag                                                        <  2:284240/139‑1 (MQ=255)
                                                                                      tACCATTGGTTAGCCTAGAAGATACAGATACTAAAGTGCTTCGTGAAACATATGACACATTTAAAGCGGGTGAAAATGAGTGAAAATATTACTAATTGGCTATGGGGCAATGAATGAACGCGTTGCAAGGTTAGCggagaa                                                       >  1:391037/1‑138 (MQ=255)
                                                                                                  gCCTAGAAGATACAGATACTAAAGTGCTTCGTGAAACATATGACACATTTAAAGCGGGTGAAAATGAGTGAAAATATTACTAATTGGCTATGGCGCAATGAATCAACGCGTTGCTAGATTAGCAGAAGAAAAAGGACATGa                                           >  1:63902/1‑141 (MQ=255)
                                                                                                    cTAGAAGATACAGATACTAAAGTGCTTCGTGAAACATATGACACATTTAAAGCGGGTGAAAATGAGTGAAAATATTACTAATTGGCTATGGCGCAATGAATCAACGCGTTGCTAGATTAGCAGAAGAAAAAGGACATGaaa                                         >  2:650876/1‑141 (MQ=255)
                                                                                                       gaagaTACAGATACTAAAGTGCTTCGTGAAACATATGACACATTTAAAGCGGGTGAAAATGAGTGAAAATATTACTAATTGGCTATGGCGCAATGAATCAACGCGTTGCTAGATTAGCAGAAGAAAAAGGACATGAAATCg                                      >  1:874543/1‑141 (MQ=255)
                                                                                                        aagaTACAGATACTAAAGTGCTTCGTGAAACATATGACACATTTAAAGCGGGTGAAAATGAGTGAAAATATTACTAATTGGCTATGGCGCAATGAATCAACGCGTTGCTAGATTAGCAGAAGAAAAAGGACATGAAATCGt                                     >  1:946350/1‑141 (MQ=255)
                                                                                                              cagatacTAAAGTGCTTCGTGAAACATATGACACATTTAAAGCGGGTGAAAATGAGTGAAAATATTACTAATTGGCTATGGCGCAATGAATCAACGCGTTGCTAGATTAGGAGAAGAAAAAGGACATGAAATCGTTgggga                               >  2:597402/1‑140 (MQ=255)
                                                                                                              cagatacTAAAGTGCTTCGTGAAACATATGACACATTTAAAGCGGGTGAAAATGAGTGAAAATATTACTAATTGGCTATGGCGCAATGAATCAACGCGTTGCTAGATTAGCAGAAGAAAAAGGACATGACATCGTTggggg                               >  1:561053/1‑140 (MQ=255)
                                                                                                                    cTAAGGTGCTGCGGGAAACATATGATACATTTAAAGCGGGTGAAAATGAGTGAAAATACTACTAATTGGCTATGGCGAAATGAATCAACGCGTTGCTAGATTAGCAGAAGAAAAAGGACATGAAATCGTTGGGGTCATCGa                         <  2:268166/141‑1 (MQ=255)
                                                                                                                      aaaGTGCTTCGTGAAACATATGACACATTTAAAGCGGGTGAAAATGAGTGAAAATATTACTAATTGGCTATGGCGCAATGAATCAACGCGTTGCTAGATTAGCAGAAGAAAAAGGACATGAAATCGTTGGGGTCATTGaaa                       >  1:432088/1‑141 (MQ=255)
                                                                                                                                  gAAACATATGGCACATTTAAAGCGGGTGAAAATGAGTGAAAATATTACTAATTGGCTATGGCGCAATGAATCAACGCGTTGCTAGATTAGCAGAAGAAAAAGGACATGAAATCGTTGGGGTCATTGAAAATACACCGAAAg           <  1:440196/141‑1 (MQ=255)
                                                                                                                                  gAAACATATGGCACATTTAAAGCGGGTGAAAATGAGTGAAAATATTACTAATTGGCTATGGCGCAATGAATCAACGCGTTGCTAGATTAGCAGAAGAAAAAGGACATGAAATCGTTGGGGTCATTGAAAATACACCGAAAg           <  2:702180/141‑1 (MQ=255)
                                                                                                                                  gAAACATATGGCACATTTAAAGCGGGTGAAAATGAGTGAAAATATTACTAATTGGCTATGGCGCAATGAATCAACGCGTCGCTAGATTAGCAGAAGAAAAAGGACATGAAATCGTTGGGGTCATTGAAAATACACCGAAAg           <  2:518453/141‑1 (MQ=255)
                                                                                                                                  gAAACATATGACACGTTTAAAGCGGGTGCAAATGAGTGAAAATATTACGAATTGGCGAGGGAGCAATGAATCAACGCGTTGCTAGATTAGAGGAAGAAAAAGAACATGAAATCGTTGGGGTCATTGAAAATacacaaacag           >  2:201224/1‑135 (MQ=255)
                                                                                                                                  gAAACATATGACACATTTAAAGCGGGTGAAAATGAGTGAAAATATTACTAATTGGCTATGGCGCAATGAATCAACGCGTTGCTAGATTAGCAGAAGAAAAAGGACATGAAATCGTTGGGGTCATTGAAAATACACCGaaaa           >  2:927626/1‑140 (MQ=255)
                                                                                                                                  gAAACATATGACACATTTAAAGCGGGTGAAAATGAGTGAAAATATTACTAATTGGCTATGGCGCAATGAATCAACGCGTTGCTAGATTAGCAGAAGAAAAAGGACATGAAATCGTTGGGGTCATTGAAAATACACCGAAAg           >  1:696252/1‑141 (MQ=255)
                                                                                                                                  gAAACATATGACACATTTAAAGCGGGTGAAAATGAGTGAAAATATTACTAATTGGCTATGGCGCAATGAATCAACGCGTTGCTAGATTAGCAGAAGAAAAAGGACATGAAATCGTTGGGGTCATTGAAAATACACCGAAAg           >  1:803264/1‑141 (MQ=255)
                                                                                                                                  gAAACATATGACACATTTAAAGCGGGTGAAAATGAGTGAAAATATTACTAATTGGCTATGGCGCAATGAATCAACGCGTTGCTAGATTAGCAGAAGAAAAAGGACATGAAATCGTTGGGGTCATTGAAAATACACCGAAAg           >  1:636002/1‑141 (MQ=255)
                                                                                                                                   aaaCATATGACACATTTAAAGCGGGTGAAAATGAGTGAAAATATTACTAATTGGCTATGGCGCAATGAATCAACGCGTTGCTAGATTAGCAGAAGAAAAAGGACATGAAATCGTTGGGGTCATTGAAAATACACCGAAAGc          <  1:55768/141‑1 (MQ=255)
                                                                                                                                     aCATATGACACATTTAAAGCGGGTGAAAATGAGTGAAAATATTACTAATTGGCTATGGCGCAATGAATCAACGCGTTGCTAGATTAGCAGAAGAAAAAGGACATGAAATCGTTGGGGTCATTGAAAATACACCGAAAGcaa        >  2:79469/1‑141 (MQ=255)
                                                                                                                                          tGACACATTTAAAGCGGGTGAAAATGAGAAACAATATTACTAATTGGCTATGGCGCAATGAATGAACGCGTTGCTAGATTAGCAGAAGAAAAAGGACATGAAATCGTTGGGGTCATTGAAAATACACAGAAAGcaaacacg   >  2:9171/1‑136 (MQ=255)
                                                                                                                                           aacacATTTAAAGCGGGTGAAAATGAGTGAAAATACTACTAATTGGCTATGGCCCAATGAATCAACGCGTTGCTAGTTGAGCAGAAGAAAAAGGACATGAAAGCGTTCGGGTCATTGAAAATACACCGAAAGCAACAACGc  <  2:222354/140‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                           
GTTATCAGCTTTATCAGTTGATATTAACCCAATTCCTATTAAAGCTCTAACAAGTTATTTAGGATTTGGAAATTATGAATTACGTCTACCATTGGTTAGCCTAGAAGATACAGATACTAAAGTGCTTCGTGAAACATATGACACATTTAAAGCGGGTGAAAATGAGTGAAAATATTACTAATTGGCTATGGCGCAATGAATCAACGCGTTGCTAGATTAGCAGAAGAAAAAGGACATGAAATCGTTGGGGTCATTGAAAATACACCGAAAGCAACAACGC  >  CP000730/1433730‑1434009

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: