Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,929,812 G→A 66.7% intergenic (‑895/‑273) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,929,8120GA66.7% 142.9 / 133.0 158intergenic (‑895/‑273)USA300HOU_1794/USA300HOU_1796hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (32/20);  new base A (49/55);  total (81/77)
Fisher's exact test for biased strand distribution p-value = 1.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.27e-01

Only 100 of 171 total aligned reads displayed.

CGCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAA  >  CP000730/1929673‑1929952
                                                                                                                                           |                                                                                                                                                   
cGCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAAtagt                                                                                                                                                    <  1:147508/141‑1 (MQ=255)
     tcTTTCTTAAATTAATAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGAGTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGt                                                                                                                                               <  2:469758/140‑1 (MQ=255)
     aCTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGt                                                                                                                                               <  2:469942/141‑1 (MQ=255)
         tCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGcc                                                                                                                                           >  2:101620/1‑141 (MQ=255)
                ttAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTAtctct                                                                                                                                    >  2:781378/1‑141 (MQ=255)
                ttAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTAtctct                                                                                                                                    >  2:744222/1‑141 (MQ=255)
                           tATTCTCATAGAATTTGTCCAATTAAGTGTAGACGACGCTATCTATCAGCTAAAATAAAAAAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGCTGATATAATAGTAGAGTAGCCTATCTCTCAGGCGGCAAt                                                                                                                         >  2:551843/1‑141 (MQ=38)
                                tcATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGa                                                                                                                    >  2:733059/1‑141 (MQ=255)
                                    aGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCa                                                                                                                >  2:883763/1‑141 (MQ=255)
                                     gAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCag                                                                                                               >  1:520782/1‑141 (MQ=255)
                                      aaTTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCaga                                                                                                              <  2:234262/141‑1 (MQ=255)
                                       aTTTGTGCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCagag                                                                                                             <  2:34544/141‑1 (MQ=255)
                                           gTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAgagga                                                                                                         >  2:829688/1‑141 (MQ=255)
                                                     gtgtAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑g                                                                                             >  1:747109/1‑141 (MQ=255)
                                                       gtAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGGGGAGGGGTA‑TAA‑G‑G‑t                                                                                         >  1:491417/1‑141 (MQ=255)
                                                             gATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GA‑Tgat                                                                                 >  2:836125/1‑139 (MQ=255)
                                                                        tCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTc                                                                     <  1:945882/141‑1 (MQ=255)
                                                                          ggCTCGAAGCATATAACTTATCAAAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGGCGCCTATCTCTCTTGCGTCAATTTCGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTcac                                                                   <  2:93428/140‑1 (MQ=255)
                                                                               gAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCAcatcat                                                              >  1:136038/1‑141 (MQ=255)
                                                                                             aTCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGtcatca                                                >  1:500526/1‑141 (MQ=255)
                                                                                             aTCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGtcatca                                                <  1:95442/141‑1 (MQ=255)
                                                                                                 atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGGACCAGTCATCAGTGGc                                           >  1:470594/15‑141 (MQ=255)
                                                                                                 atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc                                           <  1:385809/127‑1 (MQ=255)
                                                                                                 atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc                                           >  1:403493/15‑141 (MQ=255)
                                                                                                 atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc                                           >  2:233016/15‑141 (MQ=255)
                                                                                                 atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc                                           >  1:18198/15‑141 (MQ=255)
                                                                                                 atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc                                           >  1:531183/15‑141 (MQ=255)
                                                                                                  tcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCt                                          >  1:800637/14‑141 (MQ=255)
                                                                                                   cggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtg                                         >  1:809445/13‑141 (MQ=255)
                                                                                                   cggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCACTGGCtg                                         <  2:521435/129‑1 (MQ=255)
                                                                                                   cggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtg                                         <  1:273132/129‑1 (MQ=255)
                                                                                                    ggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgt                                        <  1:532799/130‑1 (MQ=255)
                                                                                                    ggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgt                                        <  1:698084/130‑1 (MQ=255)
                                                                                                     gaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                                       <  1:86919/131‑1 (MQ=255)
                                                                                                     gaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGGCATCAGTGGCtgtg                                       <  1:615758/131‑1 (MQ=255)
                                                                                                      aatgagagtagcgtGTATATTCACAGATGGTATAATAATAGGGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                                      <  2:409890/128‑1 (MQ=255)
                                                                                                      aagtagatatacTTGTATATTCACAGGTGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGCAGAGAGGAGGTGCA‑TTA‑CTT‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                                      <  2:276159/130‑1 (MQ=255)
                                                                                                       aGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                                      <  1:441428/141‑1 (MQ=255)
                                                                                                       aGTGACTGTGCTTGTATATTTGCCGATGATATAACAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                                      <  2:406851/141‑1 (MQ=255)
                                                                                                       aGTGAATATGCTTGTATATTCATAGATGGTATAATAATAGTGTCGTCTATCTCTCAGGCGTCAATT‑‑GACGAAGTGAGGAGGTGCA‑TTACT‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGct                                     <  2:213878/141‑2 (MQ=255)
                                                                                                        gtaggtatgCTTGTATATTCACAGATAGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAA‑GCATTTTCGTTCACATCATAGCACTAGTCATCAGTGGCTGTGctg                                    <  1:573061/134‑3 (MQ=255)
                                                                                                        gtagatatgattGTATCTTCACCGATGGTATAGGAATAGAGTCGCTTATCGCTCAGGGGGCAATT‑‑GACGAAGAGAGGAGGGGCA‑TTA‑C‑T‑TGGC‑TAA‑TCAGTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctg                                    <  2:890004/131‑3 (MQ=255)
                                                                                                        gtagatatgCTTGTATATTCACAGCTGGTAGAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑C‑T‑T‑TG‑CGAATCATTCTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctg                                    <  2:591319/134‑3 (MQ=255)
                                                                                                        gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctg                                    <  1:583158/134‑3 (MQ=255)
                                                                                                        gTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGcc                                     >  2:588576/1‑141 (MQ=255)
                                                                                                         tGACTGTGCTTGTATATTTGCGGATGATATAATAGGAGAGTCGCCTATCTCGCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCa                                    <  2:625450/141‑1 (MQ=255)
                                                                                                           gatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttg                                 <  1:650162/137‑6 (MQ=255)
                                                                                                           gatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttg                                 >  2:651107/5‑136 (MQ=255)
                                                                                                           gatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttg                                 >  1:128562/5‑136 (MQ=255)
                                                                                                           aCTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCAtt                                  <  2:529880/141‑1 (MQ=255)
                                                                                                            atatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttgc                                <  1:510148/138‑7 (MQ=255)
                                                                                                             tgtgCTTGTATATTTGCCGATGATATAATCGTAGCGTCGACTATCAAAAGGGAGTAAGATGATGCGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGc                                <  2:198155/141‑1 (MQ=255)
                                                                                                             tatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttgcg                               <  2:716259/139‑8 (MQ=255)
                                                                                                             tatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCg                               >  1:481777/3‑141 (MQ=255)
                                                                                                              atgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgtttcgt                              >  2:141103/2‑133 (MQ=255)
                                                                                                              atgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAA‑‑TCGACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGt                              >  1:567940/2‑141 (MQ=255)
                                                                                                                gCTTGTCTATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtat                            <  2:801468/141‑3 (MQ=255)
                                                                                                                     tataTTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatac                       <  1:439643/141‑8 (MQ=255)
                                                                                                                     tataTTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatac                       <  1:758781/141‑8 (MQ=255)
                                                                                                                     tataTTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTT‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatac                       <  2:436408/141‑8 (MQ=255)
                                                                                                                     tataTTCACAGATGGTATAATAACAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑C‑TTT‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatac                       <  1:391189/141‑8 (MQ=255)
                                                                                                                         tttGCCGATGATATAATACTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTa                    <  2:922020/141‑1 (MQ=255)
                                                                                                                         ttcacagatggtATAATAATAGAGTCGCCTATCTCTCAAGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAt                   >  1:604855/7‑141 (MQ=255)
                                                                                                                          tcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAtt                  >  2:728504/6‑141 (MQ=255)
                                                                                                                          tcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAtt                  >  2:193956/6‑141 (MQ=255)
                                                                                                                          tcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAtt                  >  2:428152/6‑141 (MQ=255)
                                                                                                                          tcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAtt                  >  1:300299/6‑141 (MQ=255)
                                                                                                                            acagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTgg                <  1:105422/138‑1 (MQ=255)
                                                                                                                             cagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGc               >  1:269137/3‑141 (MQ=255)
                                                                                                                              cGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGc               <  1:777770/141‑1 (MQ=255)
                                                                                                                              cGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCAATTGCGTTTTTTTCTTATTTGc               >  2:872463/1‑141 (MQ=255)
                                                                                                                              agatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATTGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCt              <  2:612111/140‑1 (MQ=255)
                                                                                                                              agatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCt              >  1:839811/2‑141 (MQ=255)
                                                                                                                               gatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCtt             <  2:872467/141‑2 (MQ=255)
                                                                                                                               gatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCtt             >  1:393125/1‑140 (MQ=255)
                                                                                                                               gatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCtt             >  1:234033/1‑140 (MQ=255)
                                                                                                                               gatggtATAATAACAGGGGCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCtt             <  2:730648/141‑2 (MQ=255)
                                                                                                                                atggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCtta            >  1:302500/5‑139 (MQ=255)
                                                                                                                                atggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCtta            >  1:794153/5‑139 (MQ=255)
                                                                                                                                atgatATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTa             <  2:69720/141‑1 (MQ=255)
                                                                                                                                 tggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGTGTATTATACTTATTGGCttag           >  1:750865/4‑138 (MQ=255)
                                                                                                                                 tggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttag           <  1:669884/138‑4 (MQ=255)
                                                                                                                                 tggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttag           <  1:106099/138‑4 (MQ=255)
                                                                                                                                 tggtataATAATAGAGGCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttag           <  2:941329/138‑4 (MQ=255)
                                                                                                                                 tggtataATAAGAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttag           <  2:29864/138‑4 (MQ=255)
                                                                                                                                  ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt          >  1:612586/3‑141 (MQ=255)
                                                                                                                                  ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt          >  1:204431/3‑141 (MQ=255)
                                                                                                                                  ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt          >  1:42751/3‑141 (MQ=255)
                                                                                                                                  ggtacaataatAGCGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTAGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt          <  2:566423/136‑1 (MQ=255)
                                                                                                                                  gatATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAg           <  1:649515/141‑1 (MQ=255)
                                                                                                                                   gtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTa         >  1:139950/2‑141 (MQ=255)
                                                                                                                                   gtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑C‑TTT‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGACGGGCTGTTGCGTATTATACTTATTGGCttatga         >  2:354826/2‑136 (MQ=255)
                                                                                                                                    tataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTCCA‑TTA‑C‑TTT‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGAGCTGTTGCGTATTATACTTATTGGCtgaataa        >  1:632710/1‑135 (MQ=255)
                                                                                                                                     ataataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttcgtaaa       >  2:293752/1‑134 (MQ=255)
                                                                                                                                     ataataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaa       >  1:847028/1‑139 (MQ=255)
                                                                                                                                     ataataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaa       >  2:677812/1‑139 (MQ=255)
                                                                                                                                        ataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc    <  1:184848/141‑1 (MQ=255)
                                                                                                                                        ataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATAATACGGATTGGCTTAGTAAACGc    <  1:226877/141‑1 (MQ=255)
                                                                                                                                        ataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTG‑C‑T‑T‑TACTAA‑GCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGTCTTAGTAAAccg    >  2:261080/1‑139 (MQ=255)
                                                                                                                                        ataGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACg     >  2:70133/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                                   
CGCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAA  >  CP000730/1929673‑1929952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑