Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,929,867 G→T 72.7% intergenic (‑950/‑218) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,929,8670GT72.7% 344.1 / 167.5 232intergenic (‑950/‑218)USA300HOU_1794/USA300HOU_1796hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (32/32);  new base T (88/80);  total (120/112)
Fisher's exact test for biased strand distribution p-value = 7.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 232 total aligned reads displayed.

GTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAA  >  CP000730/1929728‑1930005
                                                                                                                                               |                                                                                                                                              
          cAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTCCTTGTATATTTGCCGATGATATAATAGTAGCGTCGCCCATCTCTCCGGCGTCCATTTAGACGCAGAGAGGAGGTG‑TA‑TAG‑G‑G‑A‑GA‑TGC‑TTAtt                                                                                                                                 >  2:609540/1‑141 (MQ=255)
             tCTATCAGCTAGAATCATATAACTTCACAGAAGAAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGGG‑TA‑TAA‑G‑G‑T‑GC‑TGC‑TTCTTTTc                                                                                                                              >  2:839342/1‑141 (MQ=255)
                aTCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTTTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGtt                                                                                                                           <  1:392400/141‑1 (MQ=255)
                 tCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTc                                                                                                                          <  1:945882/141‑1 (MQ=255)
                        gAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCAcatcat                                                                                                                   >  1:136038/1‑141 (MQ=255)
                                      aTCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGtcatca                                                                                                     >  1:500526/1‑141 (MQ=255)
                                          atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc                                                                                                >  1:403493/15‑141 (MQ=255)
                                           tcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCt                                                                                               >  1:800637/14‑141 (MQ=255)
                                            tggaagtaggtatgCTTGTATATTCACAGATGGTATTATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GC‑TAA‑TCATTTTCGTTCGCATCATAGCACCAGTCATCAGTGGCtg                                                                                              <  2:220925/129‑1 (MQ=255)
                                            cggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtg                                                                                              <  1:273132/129‑1 (MQ=255)
                                              gaagtagatatgCTTGTATATTCACAGGTGGTACAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                                                                                            <  1:229031/131‑1 (MQ=255)
                                              gaagtagagtttcTTGGATATTCACAGTTGGTATAATCATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                                                                                            <  1:142484/129‑1 (MQ=255)
                                               aatgagagtagcgtGTATATTCACAGATGGTATAATAATAGGGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                                                                                           <  2:409890/128‑1 (MQ=255)
                                               aagtagatatacTTGTATATTCACAGGTGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGCAGAGAGGAGGTG‑CA‑TTA‑CTT‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                                                                                           <  2:276159/130‑1 (MQ=255)
                                                 gtagatatgattGTATCTTCACCGATGGTATAGGAATAGAGTCGCTTATCGCTCAGGGGGCAATT‑‑GACGAAGAGAGGAGGGG‑CA‑TTA‑C‑T‑TGGC‑TAA‑TCAGTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctg                                                                                         <  2:890004/131‑3 (MQ=255)
                                                 gtagatatgCTTGTATATTCACAGCTGGTAGAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑C‑T‑T‑TG‑CGAATCATTCTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctg                                                                                         <  2:591319/134‑3 (MQ=255)
                                                 gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctg                                                                                         <  1:583158/134‑3 (MQ=255)
                                                 gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑CTT‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctg                                                                                         >  2:69433/8‑139 (MQ=255)
                                                  tagatctgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTCCACATCATAGCACCAGTCATCAGTGGCTGTGctgt                                                                                        <  2:23786/135‑4 (MQ=255)
                                                   agatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑CTT‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgtt                                                                                       >  1:745034/6‑137 (MQ=255)
                                                    gatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttg                                                                                      <  1:650162/137‑6 (MQ=255)
                                                    gatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttg                                                                                      >  1:128562/5‑136 (MQ=255)
                                                    aCTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TG‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCAGATACATCATAGCACCAGTCATCAGTGGCTGTGCCAtt                                                                                       >  1:489748/1‑141 (MQ=255)
                                                    aCTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCAtt                                                                                       <  2:529880/141‑1 (MQ=255)
                                                    aCTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCAtt                                                                                       >  2:723604/1‑141 (MQ=255)
                                                     atatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttgc                                                                                     <  1:510148/138‑7 (MQ=255)
                                                      tgtgCTTGTATATTTGCCGATGATATAATCGTAGCGTCGACTATCAAAAGGGAGTAAGATGATGCGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGc                                                                                     <  2:198155/141‑1 (MQ=255)
                                                      tgtgCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGc                                                                                     <  1:755727/141‑1 (MQ=255)
                                                      tatgCTTGTATATTCACAGATGGTATAATAATCGAGTCGCCTACCTCTCAGGCGTCAATT‑‑GACGACGAGAGGAGTTG‑CA‑TTACT‑T‑T‑GC‑TAT‑GCATTTTCGTTCACATCATAGCACCCGTCATCAGTGGCTGTGctgttgcg                                                                                    <  1:689404/139‑8 (MQ=255)
                                                       atgCTTGTATATTCACAGATGGTATAATAGTAGAGTCACCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑G‑T‑T‑GC‑TAA‑TCATTTTGGTTCGCATCATAGAAACCACCATCAGTGGCTGTGctggtgcgt                                                                                   >  2:704367/2‑133 (MQ=255)
                                                       atgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATTAGTGGCGGTGCTGTTGCGt                                                                                   >  2:623953/2‑141 (MQ=255)
                                                       atgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgtttcgt                                                                                   >  2:141103/2‑133 (MQ=255)
                                                       atgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGt                                                                                   >  2:936563/2‑141 (MQ=255)
                                                       atgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAA‑‑TCGACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGt                                                                                   >  1:567940/2‑141 (MQ=255)
                                                         gCTTGTCTATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtat                                                                                 <  2:801468/141‑3 (MQ=255)
                                                         gCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtat                                                                                 <  1:97220/141‑3 (MQ=255)
                                                              tataTTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatac                                                                            <  1:758781/141‑8 (MQ=255)
                                                              tataTTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatac                                                                            <  1:439643/141‑8 (MQ=255)
                                                              tataTTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑CTT‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatac                                                                            <  2:436408/141‑8 (MQ=255)
                                                                tcttcacagatggtATAATAATAGCGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACtt                                                                          <  1:867040/133‑1 (MQ=255)
                                                                  ttcacagatggtATAATAATAGAGTCGCCTATCTCTCAAGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAt                                                                        >  1:604855/7‑141 (MQ=255)
                                                                   tcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAtt                                                                       >  1:300299/6‑141 (MQ=255)
                                                                   tcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAtt                                                                       >  2:193956/6‑141 (MQ=255)
                                                                   tcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAtt                                                                       >  2:428152/6‑141 (MQ=255)
                                                                      cagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGc                                                                    <  1:296647/139‑1 (MQ=255)
                                                                      cagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGc                                                                    <  2:353140/139‑1 (MQ=255)
                                                                      cagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCGGTGCTGTTGCGTATTATACTTATTGGc                                                                    >  1:466031/3‑141 (MQ=255)
                                                                       cGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGc                                                                    <  1:777770/141‑1 (MQ=255)
                                                                       agatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑CTT‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCt                                                                   <  2:872901/140‑1 (MQ=255)
                                                                        gatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCtt                                                                  >  1:234033/1‑140 (MQ=255)
                                                                        gatggtATAATAACAGGGGCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCtt                                                                  <  2:730648/141‑2 (MQ=255)
                                                                        gatgatATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCt                                                                   <  1:544157/141‑1 (MQ=255)
                                                                         atggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCtta                                                                 >  1:794153/5‑139 (MQ=255)
                                                                         atggtataATAATAGAGTCGCCTATCTCTCAGGCGGCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCtta                                                                 >  1:906095/5‑139 (MQ=255)
                                                                         atgatATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTa                                                                  <  2:69720/141‑1 (MQ=255)
                                                                          tggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttag                                                                <  1:531450/138‑4 (MQ=255)
                                                                          tggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttag                                                                <  1:106099/138‑4 (MQ=255)
                                                                          tgatATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTCCGTTTTTTTCTTATTGGCCaa                                                                 >  1:169448/1‑141 (MQ=255)
                                                                           ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                               >  1:612586/3‑141 (MQ=255)
                                                                           ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                               >  1:42751/3‑141 (MQ=255)
                                                                           ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                               >  1:204431/3‑141 (MQ=255)
                                                                           ggtacaataatAGCGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTAGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                               <  2:566423/136‑1 (MQ=255)
                                                                           gatATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAg                                                                >  2:494724/1‑141 (MQ=255)
                                                                             tataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTC‑CA‑TTA‑C‑TTT‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGAGCTGTTGCGTATTATACTTATTGGCtgaataa                                                             >  1:632710/1‑135 (MQ=255)
                                                                              ataataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttcgtaaa                                                            >  2:293752/1‑134 (MQ=255)
                                                                              ataataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaa                                                            >  1:847028/1‑139 (MQ=255)
                                                                              ataataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaa                                                            >  1:4748/1‑139 (MQ=255)
                                                                                 ataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc                                                         <  1:184848/141‑1 (MQ=255)
                                                                                 ataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc                                                         <  1:38992/141‑1 (MQ=255)
                                                                                 ataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTG‑C‑T‑T‑TACTAA‑GCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGTCTTAGTAAAccg                                                         >  2:261080/1‑139 (MQ=255)
                                                                                      agagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGTACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaa                                                    <  2:932192/141‑4 (MQ=255)
                                                                                      agagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaa                                                    >  2:682593/1‑138 (MQ=255)
                                                                                       gagTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAc                                                    <  2:928912/141‑1 (MQ=255)
                                                                                        agTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaat                                                  >  1:704799/1‑136 (MQ=255)
                                                                                        agTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCACTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaat                                                  <  1:625419/141‑6 (MQ=255)
                                                                                        agTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaat                                                  <  1:687557/141‑6 (MQ=255)
                                                                                           cGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataat                                               >  1:929096/1‑133 (MQ=255)
                                                                                            gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                                              >  2:743584/1‑132 (MQ=255)
                                                                                            gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                                              <  2:134626/141‑10 (MQ=255)
                                                                                             ccTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATa                                              >  2:891788/1‑141 (MQ=255)
                                                                                             ccTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTC‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTTGCTGTGCTGTTGCGTATTATGCATATTGGGTTAGTAAACGCAACAaaaactga                                             >  1:82009/1‑137 (MQ=255)
                                                                                              cTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaccaaataattaa                                            >  1:354737/1‑129 (MQ=255)
                                                                                              cTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaa                                            >  1:322458/1‑130 (MQ=255)
                                                                                              cTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaa                                            <  2:864551/141‑12 (MQ=255)
                                                                                               tATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCAA‑TAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGTCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaaa                                           >  1:526362/1‑129 (MQ=255)
                                                                                                aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGTTTAGTAAACGCaacaaataattgata                                          >  1:714068/1‑128 (MQ=255)
                                                                                                aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattcata                                          >  2:327059/1‑128 (MQ=255)
                                                                                                aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GA‑TAA‑TCATTATCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGCTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaata                                          >  2:649384/1‑128 (MQ=255)
                                                                                                aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑CTT‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaata                                          >  1:839580/1‑128 (MQ=255)
                                                                                                 tctctcATGGGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaatag                                         <  2:365915/141‑15 (MQ=255)
                                                                                                 tctctcAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTGGACGCAATACAGAAtttgtg                                          >  2:657552/1‑136 (MQ=255)
                                                                                                  ctttcAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGC‑TTA‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGAc                                        <  2:172643/138‑1 (MQ=255)
                                                                                                       atgCGTAAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGC‑TTATGTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGGGCCATTGCGGTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACatat                                    <  1:550462/139‑1 (MQ=255)
                                                                                                        ggCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGC‑TTATGTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACAtata                                   <  2:398467/141‑1 (MQ=255)
                                                                                                         gCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTTGATGCAGTACAAAATAGGTGACATATAg                                  >  1:679796/1‑141 (MQ=255)
                                                                                                              aaTTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCa                             <  1:101620/141‑1 (MQ=255)
                                                                                                                      cGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATaaa                     <  1:725120/141‑1 (MQ=255)
                                                                                                                          gagagGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATc                 >  1:441603/1‑141 (MQ=255)
                                                                                                                              ggaggTG‑TA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCt             >  1:348371/1‑141 (MQ=255)
                                                                                                                                           a‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGCCATATAGCCGCACCAATAAAAATCCCCTCACCACCGCaa  >  2:514066/1‑141 (MQ=255)
                                                                                                                                               |                                                                                                                                              
GTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAA  >  CP000730/1929728‑1930005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑