BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,929,870	A→C	72.9%	intergenic (‑953/‑215)	USA300HOU_1794 ← / → USA300HOU_1796	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,929,870	0	A	C	72.9%	439.2 / 130.6	230	intergenic (‑953/‑215)	USA300HOU_1794/USA300HOU_1796	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (32/31); new base C (87/78); total (119/111)
Fisher's exact test for biased strand distribution p-value = 8.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 232 total aligned reads displayed.

AGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAG  >  CP000730/1929730‑1930009

aGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATATCTCAGGCGTCAATTTAGTCGCAGTGAGGAGGTGTA‑TAA‑G‑G‑T‑Ga                                                                                                                                                <  1:516640/141‑1 (MQ=255)

        cAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTCCTTGTATATTTGCCGATGATATAATAGTAGCGTCGCCCATCTCTCCGGCGTCCATTTAGACGCAGAGAGGAGGTGTA‑TAG‑G‑G‑A‑GATGC‑TTAt‑‑t                                                                                                                                     >  2:609540/1‑141 (MQ=255)

           tCTATCAGCTAGAATCATATAACTTCACAGAAGAAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGGGTA‑TAA‑G‑G‑T‑GCTGC‑TTCT‑‑TTTc                                                                                                                                  >  2:839342/1‑141 (MQ=255)

              aTCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTTTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTAT‑‑TTTCGtt                                                                                                                               <  1:392400/141‑1 (MQ=255)

               tCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTAT‑‑TTTCGTTc                                                                                                                              <  1:945882/141‑1 (MQ=255)

                      gAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTAT‑‑TTTCGTTCAcatcat                                                                                                                       >  1:136038/1‑141 (MQ=255)

                                        atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGc                                                                                                    >  2:233016/15‑141 (MQ=255)

                                        atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGc                                                                                                    >  1:531183/15‑141 (MQ=255)

                                        atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGc                                                                                                    >  1:403493/15‑141 (MQ=255)

                                        atcggaagtagatatgCTTGGATGTTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGc                                                                                                    <  1:588986/127‑1 (MQ=255)

                                         tcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCt                                                                                                   >  1:800637/14‑141 (MQ=255)

                                          cggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCtg                                                                                                  >  1:809445/13‑141 (MQ=255)

                                          cggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑T‑GCTAA‑TCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCtg                                                                                                  <  1:273132/129‑1 (MQ=255)

                                           ggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgt                                                                                                 <  1:532799/130‑1 (MQ=255)

                                            gaagtagatatgCTTGTATATTCACAGGTGGTACAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                                                                                                <  1:229031/131‑1 (MQ=255)

                                            gaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCAT‑‑TTTCGTTCACATCATAGCACCAGGCATCAGTGGCtgtg                                                                                                <  1:615758/131‑1 (MQ=255)

                                            gaagtagagtttcTTGGATATTCACAGTTGGTATAATCATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                                                                                                <  1:142484/129‑1 (MQ=255)

                                             aatgagagtagcgtGTATATTCACAGATGGTATAATAATAGGGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑T‑GCTAA‑TCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                                                                                               <  2:409890/128‑1 (MQ=255)

                                              aGTGAATATGCTTGTATATTCATAGATGGTATAATAATAGTGTCGTCTATCTCTCAGGCGTCAATT‑‑GACGAAGTGAGGAGGTGCA‑TTACT‑T‑T‑GCTAA‑TCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGct                                                                                              <  2:213878/141‑2 (MQ=255)

                                               gtagatatgattGTATCTTCACCGATGGTATAGGAATAGAGTCGCTTATCGCTCAGGGGGCAATT‑‑GACGAAGAGAGGAGGGGCA‑TTA‑C‑T‑TGGCTAA‑TCAG‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctg                                                                                             <  2:890004/131‑3 (MQ=255)

                                               gtagatatgCTTGTATATTCACAGCTGGTAGAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑C‑T‑T‑TGCGAATCAT‑‑TCTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctg                                                                                             <  2:591319/134‑3 (MQ=255)

                                               gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTT‑T‑GCTAA‑TCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctg                                                                                             >  2:69433/8‑139 (MQ=255)

                                               gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGACTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCAGTAC‑T‑T‑T‑GCTAA‑TCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTCGCcgttcgt                                                                                             >  2:278690/8‑137 (MQ=255)

                                                  gatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttg                                                                                          <  1:650162/137‑6 (MQ=255)

                                                  gatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑T‑GCTAA‑TCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttg                                                                                          >  1:128562/5‑136 (MQ=255)

                                                  aCTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTG‑TAA‑G‑G‑T‑GATGC‑TTAT‑‑TTTCAGATACATCATAGCACCAGTCATCAGTGGCTGTGCCAtt                                                                                           >  1:489748/1‑141 (MQ=255)

                                                  aCTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCAtt                                                                                           <  2:529880/141‑1 (MQ=255)

                                                  aCTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCAtt                                                                                           >  2:723604/1‑141 (MQ=255)

                                                    tgtgCTTGTATATTTGCCGATGATATAATCGTAGCGTCGACTATCAAAAGGGAGTAAGATGATGCGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGc                                                                                         <  2:198155/141‑1 (MQ=255)

                                                    tgtgCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGc                                                                                         <  1:755727/141‑1 (MQ=255)

                                                    tatgCTTGTATATTCACAGATGGTATAATAATCGAGTCGCCTACCTCTCAGGCGTCAATT‑‑GACGACGAGAGGAGTTGCA‑TTACT‑T‑T‑GCTAT‑GCAT‑‑TTTCGTTCACATCATAGCACCCGTCATCAGTGGCTGTGctgttgcg                                                                                        <  1:689404/139‑8 (MQ=255)

                                                    tatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAAAT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCg                                                                                        >  1:568540/3‑141 (MQ=255)

                                                     atgCTTGTATATTCACAGATGGTATAATAGTAGAGTCACCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑G‑T‑T‑GCTAA‑TCAT‑‑TTTGGTTCGCATCATAGAAACCACCATCAGTGGCTGTGctggtgcgt                                                                                       >  2:704367/2‑133 (MQ=255)

                                                     atgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCAT‑‑TTTCGTTCACATCATAGCACCAGTCATTAGTGGCGGTGCTGTTGCGt                                                                                       >  2:623953/2‑141 (MQ=255)

                                                     atgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgtttcgt                                                                                       >  2:141103/2‑133 (MQ=255)

                                                     atgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGt                                                                                       >  2:936563/2‑141 (MQ=255)

                                                       gCTTGTCTATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtat                                                                                     <  2:801468/141‑3 (MQ=255)

                                                           gTATATTCACAGATGGTATGATAATAGAGTAGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatc                                                                                 >  1:501455/1‑138 (MQ=255)

                                                            tataTTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatac                                                                                <  1:439643/141‑8 (MQ=255)

                                                            tataTTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatac                                                                                <  1:758781/141‑8 (MQ=255)

                                                            tataTTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTT‑T‑GCTAA‑TCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatac                                                                                <  2:436408/141‑8 (MQ=255)

                                                              tcttcacagatggtATAATAATAGCGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACtt                                                                              <  1:867040/133‑1 (MQ=255)

                                                                 tcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑T‑GCTAA‑TCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAtt                                                                           >  2:193956/6‑141 (MQ=255)

                                                                 tcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑T‑GCTAA‑TCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAtt                                                                           >  2:428152/6‑141 (MQ=255)

                                                                 tcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑T‑GCTAA‑TCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAtt                                                                           >  1:300299/6‑141 (MQ=255)

                                                                    cagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGc                                                                        <  1:296647/139‑1 (MQ=255)

                                                                    cagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGc                                                                        <  2:353140/139‑1 (MQ=255)

                                                                    cagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCGGTGCTGTTGCGTATTATACTTATTGGc                                                                        >  1:466031/3‑141 (MQ=255)

                                                                     cGATGATATAATAGTAGAGTCGCCTATCACTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTAT‑‑ATTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGc                                                                        >  1:607712/1‑141 (MQ=255)

                                                                     agatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCt                                                                       >  2:270123/2‑141 (MQ=255)

                                                                     agatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTT‑T‑GCTAA‑TCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCt                                                                       <  2:872901/140‑1 (MQ=255)

                                                                      gatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCtt                                                                      >  1:234033/1‑140 (MQ=255)

                                                                      gatggtATAATAACAGGGGCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑T‑GCTAA‑TCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCtt                                                                      <  2:730648/141‑2 (MQ=255)

                                                                       atggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑T‑GCTAA‑TCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCtta                                                                     >  1:794153/5‑139 (MQ=255)

                                                                       atgatATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTa                                                                      <  2:69720/141‑1 (MQ=255)

                                                                        tggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttag                                                                    <  1:669884/138‑4 (MQ=255)

                                                                        tggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑T‑GCTAA‑TCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttag                                                                    <  1:106099/138‑4 (MQ=255)

                                                                        tggtataATAAGAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttag                                                                    <  2:29864/138‑4 (MQ=255)

                                                                        tgatATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTCCGTTTTTTTCTTATTGGCCaa                                                                     >  1:169448/1‑141 (MQ=255)

                                                                         ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                                   >  1:42751/3‑141 (MQ=255)

                                                                         ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                                   >  1:204431/3‑141 (MQ=255)

                                                                         ggtacaataatAGCGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCAT‑‑TTTAGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                                   <  2:566423/136‑1 (MQ=255)

                                                                         gatATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAg                                                                    >  2:494724/1‑141 (MQ=255)

                                                                           tataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTCCA‑TTA‑C‑TTT‑GCTAA‑TCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGAGCTGTTGCGTATTATACTTATTGGCtgaataa                                                                 >  1:632710/1‑135 (MQ=255)

                                                                            ataataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaa                                                                >  1:847028/1‑139 (MQ=255)

                                                                            ataataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑T‑GCTAA‑TCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaa                                                                >  1:4748/1‑139 (MQ=255)

                                                                             taataaTAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaac                                                               <  1:772575/141‑4 (MQ=255)

                                                                               ataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc                                                             <  1:38992/141‑1 (MQ=255)

                                                                               ataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc                                                             <  1:184848/141‑1 (MQ=255)

                                                                                  atagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCTGTTGCTTTGCTGTTGCGTATTATATTTATTGGCTTATTAAACGCaac                                                          >  2:147231/2‑140 (MQ=255)

                                                                                   tagAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTAT‑‑GTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTACTTAGACGCAAt                                                          >  2:259973/1‑141 (MQ=255)

                                                                                    agagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCAT‑‑TTTCGTTCACATCATAGTACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaa                                                        <  2:932192/141‑4 (MQ=255)

                                                                                    agagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaa                                                        >  2:682593/1‑138 (MQ=255)

                                                                                     gagTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAc                                                        <  2:928912/141‑1 (MQ=255)

                                                                                      agTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑T‑GCTAA‑TCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaat                                                      <  1:687557/141‑6 (MQ=255)

                                                                                         ggCCTATCTCTCCGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTC‑C‑T‑T‑‑‑TGC‑TAATCATTTCGTTCACATCATAGCACCACTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGTCTTAGTAAACGCaacaaataatt                                                  <  2:337322/140‑10 (MQ=255)

                                                                                         cGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataat                                                   >  1:929096/1‑133 (MQ=255)

                                                                                          gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                                                  >  2:743584/1‑132 (MQ=255)

                                                                                          gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑T‑GCTAA‑TCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                                                  <  2:134626/141‑10 (MQ=255)

                                                                                           ccTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATa                                                  >  2:891788/1‑141 (MQ=255)

                                                                                           ccTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatta                                                 >  1:548759/1‑131 (MQ=255)

                                                                                            cTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaccaaataattaa                                                >  1:354737/1‑129 (MQ=255)

                                                                                            cTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaa                                                <  2:864551/141‑12 (MQ=255)

                                                                                            cTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaa                                                >  1:322458/1‑130 (MQ=255)

                                                                                              aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGTTTAGTAAACGCaacaaataattgata                                              >  1:714068/1‑128 (MQ=255)

                                                                                              aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaatg                                              >  1:699730/1‑128 (MQ=255)

                                                                                              aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCAT‑‑TTTCGTGCACATCATAGCACCAGTCATGAGTGGCTGTGCTGTTGCGTATTATACTGATTGGCTTAGTAGACGCaacaaataattaaaa                                              >  1:95698/1‑128 (MQ=255)

                                                                                              aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑T‑GATAA‑TCAT‑‑TATCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGCTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaata                                              >  2:649384/1‑128 (MQ=255)

                                                                                              aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTT‑T‑GCTAA‑TCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaata                                              >  1:839580/1‑128 (MQ=255)

                                                                                               tctctcATGGGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaatag                                             <  2:365915/141‑15 (MQ=255)

                                                                                               tctctcAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTGGACGCAATACAGAAtttgtg                                              >  2:657552/1‑136 (MQ=255)

                                                                                               tctctcAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAA‑TCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaatag                                             >  1:553255/1‑127 (MQ=255)

                                                                                                    cAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAAGACAAAATAGGTGACata                                         >  1:736943/1‑141 (MQ=255)

                                                                                                      ggCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTAT‑‑GTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACAtata                                       <  2:398467/141‑1 (MQ=255)

                                                                                                            aaTTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCa                                 <  1:101620/141‑1 (MQ=255)

                                                                                                                  gACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATa                           <  1:883763/141‑1 (MQ=255)

                                                                                                                    cGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATaaa                         <  1:725120/141‑1 (MQ=255)

                                                                                                                        gagagGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATc                     >  1:441603/1‑141 (MQ=255)

                                                                                                                            ggaggTGTA‑TAA‑G‑G‑T‑GATGC‑TTAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCt                 >  1:348371/1‑141 (MQ=255)

                                                                                                                                        a‑G‑G‑T‑GATGC‑TTAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGCCATATAGCCGCACCAATAAAAATCCCCTCACCACCGCaa      >  2:514066/1‑141 (MQ=255)

AGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGC‑TTAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAG  >  CP000730/1929730‑1930009

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`