Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,929,872 G→A 72.1% intergenic (‑955/‑213) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,929,8720GA72.1% 315.6 / 163.7 231intergenic (‑955/‑213)USA300HOU_1794/USA300HOU_1796hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (31/33);  new base A (88/78);  total (119/112)
Fisher's exact test for biased strand distribution p-value = 5.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.24e-01

Only 100 of 232 total aligned reads displayed.

GATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTG  >  CP000730/1929734‑1930011
                                                                                                                                                 |                                                                                                                                            
    cAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTCCTTGTATATTTGCCGATGATATAATAGTAGCGTCGCCCATCTCTCCGGCGTCCATTTAGACGCAGAGAGGAGGTG‑TA‑TAG‑G‑G‑A‑GA‑TGC‑TTAtt                                                                                                                                       >  2:609540/1‑141 (MQ=255)
       tCTATCAGCTAGAATCATATAACTTCACAGAAGAAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGGG‑TA‑TAA‑G‑G‑T‑GC‑TGC‑TTCTTTTc                                                                                                                                    >  2:839342/1‑141 (MQ=255)
          aTCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTTTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGtt                                                                                                                                 <  1:392400/141‑1 (MQ=255)
                  gAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCAcatcat                                                                                                                         >  1:136038/1‑141 (MQ=255)
                                aTCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGtcatca                                                                                                           <  1:95442/141‑1 (MQ=255)
                                    atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc                                                                                                      >  1:403493/15‑141 (MQ=255)
                                      tggaagtaggtatgCTTGTATATTCACAGATGGTATTATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GC‑TAA‑TCATTTTCGTTCGCATCATAGCACCAGTCATCAGTGGCtg                                                                                                    <  2:220925/129‑1 (MQ=255)
                                      cggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtg                                                                                                    <  1:273132/129‑1 (MQ=255)
                                       ggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgt                                                                                                   <  1:698084/130‑1 (MQ=255)
                                        gaagtagatatgCTTGTATATTCACAGGTGGTACAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                                                                                                  <  1:229031/131‑1 (MQ=255)
                                        gaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                                                                                                  <  1:86919/131‑1 (MQ=255)
                                        gaagtagagtttcTTGGATATTCACAGTTGGTATAATCATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                                                                                                  <  1:142484/129‑1 (MQ=255)
                                          aGTGACTGTGCTTGTATATTTGCCGATGATATAACAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                                                                                                 <  2:406851/141‑1 (MQ=255)
                                           gtaggtatgCTTGTATATTCACAGATAGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑GCATTTTCGTTCACATCATAGCACTAGTCATCAGTGGCTGTGctg                                                                                               <  1:573061/134‑3 (MQ=255)
                                           gtagatatgattGTATCTTCACCGATGGTATAGGAATAGAGTCGCTTATCGCTCAGGGGGCAATT‑‑GACGAAGAGAGGAGGGG‑CA‑TTA‑C‑T‑TGGC‑TAA‑TCAGTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctg                                                                                               <  2:890004/131‑3 (MQ=255)
                                           gtagatatgCTTGTATATTCACAGCTGGTAGAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑C‑T‑T‑TG‑CGAATCATTCTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctg                                                                                               <  2:591319/134‑3 (MQ=255)
                                           gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑CTT‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctg                                                                                               >  2:69433/8‑139 (MQ=255)
                                            tagatctgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTCCACATCATAGCACCAGTCATCAGTGGCTGTGctgt                                                                                              <  2:23786/135‑4 (MQ=255)
                                             agatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATCGCACCAGTCATCACTGGCTgcgctgtt                                                                                             >  1:799861/6‑137 (MQ=255)
                                             agatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑CTT‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgtt                                                                                             >  1:745034/6‑137 (MQ=255)
                                              gatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttg                                                                                            >  1:128562/5‑136 (MQ=255)
                                              aCTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TG‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCAGATACATCATAGCACCAGTCATCAGTGGCTGTGCCAtt                                                                                             >  1:489748/1‑141 (MQ=255)
                                              aCTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCAtt                                                                                             <  2:529880/141‑1 (MQ=255)
                                              aCTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCAtt                                                                                             >  2:723604/1‑141 (MQ=255)
                                                 atgCTTGTATATTCACAGATGGTATAATAGTAGAGTCACCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑G‑T‑T‑GC‑TAA‑TCATTTTGGTTCGCATCATAGAAACCACCATCAGTGGCTGTGctggtgcgt                                                                                         >  2:704367/2‑133 (MQ=255)
                                                 atgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATTAGTGGCGGTGCTGTTGCGt                                                                                         >  2:623953/2‑141 (MQ=255)
                                                 atgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGt                                                                                         >  2:936563/2‑141 (MQ=255)
                                                 atgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAA‑‑TCGACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGt                                                                                         >  1:567940/2‑141 (MQ=255)
                                                   gCTTGTCTATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtat                                                                                       <  2:801468/141‑3 (MQ=255)
                                                   gCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtat                                                                                       <  1:97220/141‑3 (MQ=255)
                                                    cTTGGATATTCACAGATGGTAAAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑C‑TTT‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatt                                                                                      <  1:803171/141‑1 (MQ=255)
                                                       gTATATTCACAGATGGTATGATAATAGAGTAGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatc                                                                                   >  1:501455/1‑138 (MQ=255)
                                                       gTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattata                                                                                   <  1:658777/141‑7 (MQ=255)
                                                       gTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattata                                                                                   <  2:144288/141‑7 (MQ=255)
                                                        tataTTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatac                                                                                  <  1:439643/141‑8 (MQ=255)
                                                         atattcaaagatggtATAATAATAGAGTCGCCTCTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatact                                                                                 >  2:344770/10‑136 (MQ=255)
                                                            ttcacagatggtATAATAATAGAGTCGCCTATCTCTCAAGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAt                                                                              >  1:604855/7‑141 (MQ=255)
                                                             tcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAtt                                                                             >  1:300299/6‑141 (MQ=255)
                                                              cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑CTT‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTg                                                                            >  2:206439/5‑141 (MQ=255)
                                                                cagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGc                                                                          <  1:296647/139‑1 (MQ=255)
                                                                cagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCGGTGCTGTTGCGTATTATACTTATTGGc                                                                          >  1:466031/3‑141 (MQ=255)
                                                                 cGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGc                                                                          <  1:777770/141‑1 (MQ=255)
                                                                 agatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCt                                                                         >  1:839811/2‑141 (MQ=255)
                                                                 agatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑CTT‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCt                                                                         <  2:872901/140‑1 (MQ=255)
                                                                  gatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCtt                                                                        >  1:234033/1‑140 (MQ=255)
                                                                  gatggtATAATAACAGGGGCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCtt                                                                        <  2:730648/141‑2 (MQ=255)
                                                                   atggtataATAATAGAGTCGCCTATCTCTCAGGCGGCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCtta                                                                       >  1:906095/5‑139 (MQ=255)
                                                                   atgatATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTa                                                                        <  2:69720/141‑1 (MQ=255)
                                                                    tggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttag                                                                      <  1:531450/138‑4 (MQ=255)
                                                                    tggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttag                                                                      <  1:106099/138‑4 (MQ=255)
                                                                    tgatATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTCCGTTTTTTTCTTATTGGCCaa                                                                       >  1:169448/1‑141 (MQ=255)
                                                                     ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                                     >  1:42751/3‑141 (MQ=255)
                                                                     ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                                     >  1:204431/3‑141 (MQ=255)
                                                                     ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                                     >  1:612586/3‑141 (MQ=255)
                                                                     ggtacaataatAGCGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTAGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                                     <  2:566423/136‑1 (MQ=255)
                                                                     gatATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAg                                                                      <  1:649515/141‑1 (MQ=255)
                                                                      gtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑C‑TTT‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGACGGGCTGTTGCGTATTATACTTATTGGCttatga                                                                    >  2:354826/2‑136 (MQ=255)
                                                                        ataataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttcgtaaa                                                                  >  2:293752/1‑134 (MQ=255)
                                                                        ataataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaa                                                                  >  1:4748/1‑139 (MQ=255)
                                                                           ataatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTCC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCGGTCAGCAGCGGCTTTGCTGTTGCGTATTATTCTTATTGGCTTAGTAAACGc                                                               >  2:198069/5‑141 (MQ=255)
                                                                           ataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc                                                               <  1:38992/141‑1 (MQ=255)
                                                                           ataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc                                                               <  1:184848/141‑1 (MQ=255)
                                                                           ataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTG‑C‑T‑T‑TACTAA‑GCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGTCTTAGTAAAccg                                                               >  2:261080/1‑139 (MQ=255)
                                                                              gtagAGACGACTATCTCTCAGGCGCCAAGTTAGACGCAGAGGGGATGTG‑TA‑TAA‑G‑G‑G‑GA‑TTC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCaa                                                             <  2:414113/141‑1 (MQ=255)
                                                                                agagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGTACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaa                                                          <  2:932192/141‑4 (MQ=255)
                                                                                agagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaa                                                          >  1:864551/1‑138 (MQ=255)
                                                                                agagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaa                                                          >  2:682593/1‑138 (MQ=255)
                                                                                 gagTCGCCTATCTTTCATGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑CTT‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaa                                                         <  2:378383/141‑5 (MQ=255)
                                                                                 gagTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAc                                                          <  2:928912/141‑1 (MQ=255)
                                                                                 gagTCGCCTAGCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaa                                                         <  1:733450/141‑5 (MQ=255)
                                                                                  agTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaat                                                        >  1:704799/1‑136 (MQ=255)
                                                                                  agTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCACTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaat                                                        <  1:625419/141‑6 (MQ=255)
                                                                                   gTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaata                                                       <  1:716526/141‑7 (MQ=255)
                                                                                   gTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACAAGACATCAGTTGCTGTGCTGTTGCGTATTATACTTGTTGGCTTAGTAAACGCaacaaata                                                       <  1:689121/141‑7 (MQ=255)
                                                                                     cGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataat                                                     >  2:139467/1‑133 (MQ=255)
                                                                                     cGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataat                                                     >  2:272426/1‑133 (MQ=255)
                                                                                      gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                                                    >  2:743584/1‑132 (MQ=255)
                                                                                      gCCGATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TTA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAAt                                                     <  2:494823/141‑1 (MQ=255)
                                                                                       ccTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATa                                                    >  2:891788/1‑141 (MQ=255)
                                                                                       ccTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTC‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTTGCTGTGCTGTTGCGTATTATGCATATTGGGTTAGTAAACGCAACAaaaactga                                                   >  1:82009/1‑137 (MQ=255)
                                                                                        cTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaccaaataattaa                                                  >  1:354737/1‑129 (MQ=255)
                                                                                        cTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaa                                                  >  1:322458/1‑130 (MQ=255)
                                                                                        cTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaa                                                  <  2:864551/141‑12 (MQ=255)
                                                                                        cTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaa                                                  >  1:769679/1‑130 (MQ=255)
                                                                                         tATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCAA‑TAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGTCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaaa                                                 >  1:526362/1‑129 (MQ=255)
                                                                                          aTCTCTCAGGCGTCACTTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGt                                                 >  1:937040/1‑141 (MQ=255)
                                                                                          aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAATGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaata                                                >  1:542668/1‑128 (MQ=255)
                                                                                          aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GC‑TAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattcata                                                >  2:327059/1‑128 (MQ=255)
                                                                                          aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GA‑TAA‑TCATTATCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGCTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaata                                                >  2:649384/1‑128 (MQ=255)
                                                                                           tctctcAGGCGTCAATTTAGACGCAGAGAGGCGGCG‑TA‑CAA‑G‑G‑T‑GA‑TGC‑TTATTTTAGTTCACAACATAGAACCAGTCATCAGTGGATGTGCCATTGCGTTTTTTGCTTATTGGCTAAGTTGGCGCAATCCAAACTAGGTg                                                >  2:453324/1‑141 (MQ=255)
                                                                                           tctctcAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTGGACGCAATACAGAAtttgtg                                                >  2:657552/1‑136 (MQ=255)
                                                                                            ctttcAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGC‑TTA‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGAc                                              <  2:172643/138‑1 (MQ=255)
                                                                                                cAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCAGAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACata                                           <  2:428250/141‑1 (MQ=255)
                                                                                                 atgCGTAAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGC‑TTATGTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGGGCCATTGCGGTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACatat                                          <  1:550462/139‑1 (MQ=255)
                                                                                                   gCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTTGATGCAGTACAAAATAGGTGACATATAg                                        >  1:679796/1‑141 (MQ=255)
                                                                                                        aaTTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCa                                   <  1:101620/141‑1 (MQ=255)
                                                                                                                    gagagGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATc                       >  1:441603/1‑141 (MQ=255)
                                                                                                                        ggaggTG‑TA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCt                   >  1:348371/1‑141 (MQ=255)
                                                                                                                                     a‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGCCATATAGCCGCACCAATAAAAATCCCCTCACCACCGCaa        >  2:514066/1‑141 (MQ=255)
                                                                                                                                     a‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCaa        >  1:757352/1‑141 (MQ=255)
                                                                                                                                                 |                                                                                                                                            
GATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTG  >  CP000730/1929734‑1930011

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑