BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,929,918	A→G	63.7%	intergenic (‑1001/‑167)	USA300HOU_1794 ← / → USA300HOU_1796	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,929,918	0	A	G	63.7%	210.9 / 170.3	195	intergenic (‑1001/‑167)	USA300HOU_1794/USA300HOU_1796	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (34/37); new base G (73/51); total (107/88)
Fisher's exact test for biased strand distribution p-value = 1.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 196 total aligned reads displayed.

TGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGA‑TGCTTAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTA  >  CP000730/1929778‑1930053

  aCTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGA‑TGCTTAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCAtt                                                                                                                                       <  2:529880/141‑1 (MQ=255)

    tgtgCTTGTATATTTGCCGATGATATAATCGTAGCGTCGACTATCAAAAGGGAGTAAGATGATGCGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGA‑TGCTTAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGc                                                                                                                                     <  2:198155/141‑1 (MQ=255)

    tatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑TGC‑TAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCg                                                                                                                                    >  1:481777/3‑141 (MQ=255)

     ttgCTTGTATATTCACAGGTGTGATCATAATAGAGGCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGC‑TAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGt                                                                                                                                   <  1:352086/140‑1 (MQ=255)

     atgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGC‑TAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATTAGTGGCGGTGCTGTTGCGt                                                                                                                                   >  2:623953/2‑141 (MQ=255)

     atgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAA‑‑TCGACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGC‑TAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGt                                                                                                                                   >  1:567940/2‑141 (MQ=255)

       gCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGC‑TAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtat                                                                                                                                 <  1:97220/141‑3 (MQ=255)

       gCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGC‑TAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtat                                                                                                                                 <  1:271449/141‑3 (MQ=255)

        cTTGGATATTCACAGATGGTAAAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑C‑TTTGC‑TAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatt                                                                                                                                <  1:803171/141‑1 (MQ=255)

           gTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGC‑TAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattata                                                                                                                             <  1:658777/141‑7 (MQ=255)

           gTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGC‑TAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattata                                                                                                                             <  2:144288/141‑7 (MQ=255)

            tataTTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGC‑TAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatac                                                                                                                            <  1:439643/141‑8 (MQ=255)

            tataTTCACAGATGGTATAATAACAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑C‑TTTGC‑TAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatac                                                                                                                            <  1:391189/141‑8 (MQ=255)

                ttcacagatggtATAATAATAGAGTCGCCTATCTCTCAAGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGC‑TAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAt                                                                                                                        >  1:604855/7‑141 (MQ=255)

                 tcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGC‑TAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAtt                                                                                                                       >  2:728504/6‑141 (MQ=255)

                 tcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑TGC‑TAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAtt                                                                                                                       >  1:300299/6‑141 (MQ=255)

                 tcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑TGC‑TAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAtt                                                                                                                       >  2:193956/6‑141 (MQ=255)

                  cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑CTT‑TGC‑TAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTg                                                                                                                      >  2:206439/5‑141 (MQ=255)

                   acagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑TGC‑TAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTgg                                                                                                                     <  1:105422/138‑1 (MQ=255)

                    cagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGC‑TAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGc                                                                                                                    <  1:296647/139‑1 (MQ=255)

                    cagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGC‑TAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCGGTGCTGTTGCGTATTATACTTATTGGc                                                                                                                    >  1:466031/3‑141 (MQ=255)

                     cGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGA‑TGCTTAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCAATTGCGTTTTTTTCTTATTTGc                                                                                                                    >  2:872463/1‑141 (MQ=255)

                     agatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGC‑TAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCt                                                                                                                   >  2:270123/2‑141 (MQ=255)

                     agatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑CTT‑TGC‑TAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCt                                                                                                                   <  2:872901/140‑1 (MQ=255)

                      gatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGC‑TAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCtt                                                                                                                  >  1:234033/1‑140 (MQ=255)

                      gatgatATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGA‑TGCTTAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCt                                                                                                                   <  1:544157/141‑1 (MQ=255)

                       atgatATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGA‑TGCTTAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTa                                                                                                                  <  2:69720/141‑1 (MQ=255)

                        tggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGC‑TAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGTGTATTATACTTATTGGCttag                                                                                                                >  1:750865/4‑138 (MQ=255)

                        tggtataATAATAGAGGCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGC‑TAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttag                                                                                                                <  2:941329/138‑4 (MQ=255)

                        tggtataATAAGAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGC‑TAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttag                                                                                                                <  2:29864/138‑4 (MQ=255)

                         ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGC‑TAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                                                                               >  1:612586/3‑141 (MQ=255)

                         gatATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGA‑TGCTTAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAg                                                                                                                >  2:494724/1‑141 (MQ=255)

                          gtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGC‑TAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTa                                                                                                              >  1:139950/2‑141 (MQ=255)

                          gtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑C‑TTTGC‑TAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGACGGGCTGTTGCGTATTATACTTATTGGCttatga                                                                                                              >  2:354826/2‑136 (MQ=255)

                           tataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTC‑CA‑TTA‑C‑TTTGC‑TAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGAGCTGTTGCGTATTATACTTATTGGCtgaataa                                                                                                             >  1:632710/1‑135 (MQ=255)

                            ataataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGC‑TAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaa                                                                                                            >  1:847028/1‑139 (MQ=255)

                            ataataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑TGC‑TAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaa                                                                                                            >  2:677812/1‑139 (MQ=255)

                             taataagagcggcGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑TGC‑TAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAAc                                                                                                           <  1:42065/129‑1 (MQ=255)

                             taataaTAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGC‑TAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaac                                                                                                           <  1:772575/141‑4 (MQ=255)

                               ataatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑TGC‑TAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc                                                                                                         <  1:185103/137‑1 (MQ=255)

                               ataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGC‑TAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc                                                                                                         <  1:184848/141‑1 (MQ=255)

                               ataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGC‑TAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATAATACGGATTGGCTTAGTAAACGc                                                                                                         <  1:226877/141‑1 (MQ=255)

                               ataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTG‑C‑T‑TTACTAAGCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGTCTTAGTAAAccg                                                                                                         >  2:261080/1‑139 (MQ=255)

                                  gtagAGACGACTATCTCTCAGGCGCCAAGTTAGACGCAGAGGGGATGTG‑TA‑TAA‑G‑G‑GGA‑TTCTTAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCaa                                                                                                       <  2:414113/141‑1 (MQ=255)

                                  atagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGC‑TAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaac                                                                                                      >  2:633990/2‑140 (MQ=255)

                                   tagAGTCGCCTATCTCTCAGGCGTCAATGTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGA‑TGCTTAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAAt                                                                                                      <  1:346058/141‑1 (MQ=255)

                                   tagAGCCGTCTATTTCTCAGTCGGCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑C‑TTTGC‑TAATCAT‑‑TTTCGTTCACATCATAGCACCAGTTATCAGCGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaaca                                                                                                     <  2:852145/141‑3 (MQ=255)

                                    agagTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGA‑TGCTTAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATa                                                                                                     <  1:913395/141‑1 (MQ=255)

                                    agagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGC‑TAATCAT‑‑TTTCGTTCACATCATAGTACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaa                                                                                                    <  2:932192/141‑4 (MQ=255)

                                    agagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGC‑TAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaa                                                                                                    >  1:864551/1‑138 (MQ=255)

                                     gagTCGCCTATCTTTCATGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑CTT‑TGC‑TAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaa                                                                                                   <  2:378383/141‑5 (MQ=255)

                                     gagTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGA‑TGCTTAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAc                                                                                                    <  2:928912/141‑1 (MQ=255)

                                     gagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGC‑TAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCAGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAAGGCaacaaa                                                                                                   >  1:68318/1‑137 (MQ=255)

                                     gagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑CTT‑TGC‑TAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaagaat                                                                                                   >  1:442004/1‑137 (MQ=255)

                                     gagTCGCCTAGCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGC‑TAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaa                                                                                                   <  1:733450/141‑5 (MQ=255)

                                      agTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGA‑TGCTTAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACa                                                                                                   >  2:60435/1‑141 (MQ=255)

                                      agTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGC‑TAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaat                                                                                                  >  1:704799/1‑136 (MQ=255)

                                      agTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGC‑TAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCACTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaat                                                                                                  <  1:625419/141‑6 (MQ=255)

                                       gTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGC‑TAATCAT‑‑TTTCGTTCACATCATAGCACAAGACATCAGTTGCTGTGCTGTTGCGTATTATACTTGTTGGCTTAGTAAACGCaacaaata                                                                                                 <  1:689121/141‑7 (MQ=255)

                                         ggCCTATCTCTCCGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTC‑C‑T‑T‑‑‑TGCTAATCATTTCGTTCACATCATAGCACCACTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGTCTTAGTAAACGCaacaaataatt                                                                                              <  2:337322/140‑10 (MQ=255)

                                          gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGC‑TAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                                                                                              >  2:743584/1‑132 (MQ=255)

                                          gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGC‑TAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                                                                                              <  2:718271/141‑10 (MQ=255)

                                          gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑TGC‑TAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                                                                                              <  2:134626/141‑10 (MQ=255)

                                          gCCTATCTCTCAGGCGGCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑TGC‑TAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                                                                                              <  1:49383/141‑10 (MQ=255)

                                          gCCGATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TTA‑G‑G‑TGA‑TGCTTAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAAt                                                                                               <  2:494823/141‑1 (MQ=255)

                                           ccTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGA‑TGCTTAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGCAGACGCAGTACaaaaga                                                                                              >  2:891759/1‑139 (MQ=255)

                                            cTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGC‑TAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattca                                                                                            >  2:689590/1‑130 (MQ=255)

                                             tATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCAA‑TAC‑T‑T‑TGC‑TAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGTCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaaa                                                                                           >  1:526362/1‑129 (MQ=255)

                                              aTCTCTCAGGCGTCACTTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGA‑TGCTTAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGt                                                                                           >  1:937040/1‑141 (MQ=255)

                                              aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGC‑TAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGTTTAGTAAACGCaacaaataattgata                                                                                          >  1:714068/1‑128 (MQ=255)

                                              aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGC‑TAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaata                                                                                          <  2:800637/141‑14 (MQ=255)

                                              aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGC‑TAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaata                                                                                          >  1:167979/1‑128 (MQ=255)

                                              aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGC‑TAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAATGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaata                                                                                          >  1:542668/1‑128 (MQ=255)

                                              aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑TGA‑TAATCAT‑‑TATCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGCTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaata                                                                                          >  2:649384/1‑128 (MQ=255)

                                              aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑CTT‑TGC‑TAATCAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaata                                                                                          >  1:839580/1‑128 (MQ=255)

                                               tctctcAGGCGTCAATTTAGACGCAGAGAGGCGGCG‑TA‑CAA‑G‑G‑TGA‑TGCTTAT‑‑TTTAGTTCACAACATAGAACCAGTCATCAGTGGATGTGCCATTGCGTTTTTTGCTTATTGGCTAAGTTGGCGCAATCCAAACTAGGTg                                                                                          >  2:453324/1‑141 (MQ=255)

                                                ctttcAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGA‑TGCTTA‑‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGAc                                                                                        <  2:172643/138‑1 (MQ=255)

                                                    cAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGA‑TGCTTAT‑‑TTTCGTTCACATCAGAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACata                                                                                     <  2:428250/141‑1 (MQ=255)

                                                     atgCGTAAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGA‑TGCTTAT‑‑GTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGGGCCATTGCGGTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACatat                                                                                    <  1:550462/139‑1 (MQ=255)

                                                      ggCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGA‑TGCTTAT‑‑GTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACAtata                                                                                   <  2:398467/141‑1 (MQ=255)

                                                        cGTTAATTTAGACGCAGGGAGGAGGTG‑TA‑TAA‑G‑G‑TGA‑TGCTTAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGc                                                                                 <  1:309534/141‑1 (MQ=255)

                                                         gTCAATTTTGACGCAGGGAGGAAGTT‑GA‑TAT‑G‑G‑TGA‑TGCTTAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGGGCTATTTCGTTTTTTTCTTATTGGCTAAGTATACGCAATACAATATAGGTGACATATAGcc                                                                                <  2:90529/141‑1 (MQ=255)

                                                            aaTTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGA‑TGCTTAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCa                                                                             <  1:101620/141‑1 (MQ=255)

                                                              tttAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑GGA‑TGCTTAT‑‑GTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGAAATACACCATAGGAGACATATAGCCGCAcc                                                                           <  2:693638/141‑1 (MQ=255)

                                                                tAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGA‑TGCTTAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCACTACAAAATAGGTGACATATAGCCGCCCCaa                                                                         >  1:377853/1‑141 (MQ=255)

                                                                  gACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGA‑TGCTTAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATa                                                                       <  1:781378/141‑1 (MQ=255)

                                                                  gACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGA‑TGCTTAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATa                                                                       <  1:883763/141‑1 (MQ=255)

                                                                                         a‑G‑G‑TGA‑TGCTTAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGCCATATAGCCGCACCAATAAAAATCCCCTCACCACCGCaa                                                  >  2:514066/1‑141 (MQ=255)

                                                                                         a‑G‑G‑TGA‑TGCTTAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCaa                                                  >  1:757352/1‑141 (MQ=255)

                                                                                                gA‑TGCTTAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATGAAAAATAGGTGACAAATAGCCGCACCAATAAAAATCCCCTCACTAACGCAAATAg                                              >  2:345692/1‑141 (MQ=255)

                                                                                                                              cccAGTCATCAGTGGCTGGGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATAc                   <  1:495684/140‑1 (MQ=255)

                                                                                                                               ccAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACt                  <  1:723604/141‑1 (MQ=255)

                                                                                                                                    catcaGTGGCTGTGCCATTGCGTTTTTTTCTTGTTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTAttt             <  2:22524/141‑1 (MQ=255)

                                                                                                                                    catcaGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATGCTTAttt             <  1:588576/141‑1 (MQ=255)

                                                                                                                                        aGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGt         >  1:11169/1‑141 (MQ=255)

                                                                                                                                        aGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGt         >  2:573681/1‑141 (MQ=255)

                                                                                                                                            gCTGTGCCATTGCGTCTTTTTCTCATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTc     <  1:764350/141‑1 (MQ=255)

                                                                                                                                               gtgCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTa  >  2:286405/1‑141 (MQ=255)

                                                                                                                                               gtgCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTa  <  1:677448/141‑1 (MQ=255)

                                                                                                                                               gtgCCATTGCGTTTTTTTCTTATTGGCTAAGGAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTa  <  1:829688/141‑1 (MQ=255)

TGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGA‑TGCTTAT‑‑TTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTA  >  CP000730/1929778‑1930053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`