Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,331,676 A→G 5.4% Y81Y (TAT→TAC lacC ← tagatose‑6‑phosphate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,331,6760AG 5.4% 242.7 / 2.9 88Y81Y (TAT→TAClacCtagatose‑6‑phosphate kinase
Reads supporting (aligned to +/- strand):  ref base A (43/40);  new base G (2/3);  total (45/43)
Fisher's exact test for biased strand distribution p-value = 6.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01

TGTTCAAAATGTTTAATAAATCCTGCAGCCTCTTGATTATCAATCTCCGGTCCCTGCTCTAAAATTTCTGTTTGTTGCCCTTCATGTAAAATTGCAATGCAGTTTCGTGTTTCACCCTTAATGTTATAAAATGCATGCTTGATGTCGGCATGATCTAATTTTTTAGCAATAAATTGACCTAATTCACCGCCAATAAAACCACTCGCAAGGACTGGCTCACCTACTTGCGCAAGTACTCTTGTTACATTTAAACCTTTACCACCAGC  >  CP000730/2331551‑2331816
                                                                                                                             |                                                                                                                                            
tgtttgaaaTGTTTAATATCTCCTGCAGCCTCTTGATTCTCGATCTCCGGTCCCTGCTCTAAACTTTTTGTTTTTTGCCCTTCATGTAAAATTGCAATGCATTTTCGTGTTTCAACCTTAATGTTATAAAATGCATGCTTg                                                                                                                               <  2:790519/135‑1 (MQ=255)
   tCAAAATGTTTAATAAATCCTGCAGCCTCTTGATTATCAATCTCCGGTCCCTGCTCTAAAATTTCTGTTTGTTGCCCTTCATGTAAAATTGCAATGCAGTTTCGTGTTTCACCCTTAATGTTATAAAATGCATGCTTGATg                                                                                                                            >  2:863334/1‑141 (MQ=255)
   tCAAAATGTTTAATAAATCCTGCAGCCTCTTGATTATCAATCTCCGGTCCCTGCTCTAAAATTTCTGTTTGTTGCCCTTCATGTAAAATTGCAATGAAGTTTCGTGTTTCACCCTTAGTGTTATAAAATGAATGCTTGAtt                                                                                                                            >  2:281266/1‑140 (MQ=255)
    cAAATTGTTTAATGAATCCTGCAGCCTCTTGATTATTAATCTCCGGTCTCTGCTCTAAAATTTCTGTTTGTTGCCCTTCATGTAAAATTGCAATGCAGTTTCGTGTTTCACCCTTAATGTTATAAAATGCATGCTTGATGt                                                                                                                           <  2:30020/141‑1 (MQ=255)
      aaaTGTTTAATAAATCCTGCACCCTCTTGATTATCAATCTCCGGTCCCTGCTCTAAAATTTCTGTTTGTTGCCCTTCATGTAAAATTGCAATGCAGTTTCGTGTTTCACCCTTAATGTTATAAAATGCATGCTTGATGTCg                                                                                                                         <  1:756434/141‑1 (MQ=255)
        aTGTTTAATAAATCCTGCAGCCTCTTGATTATCAATCTCCGGTCCCTGCTCTAAAATTTCTGTTTGTTGCCCTTCATGTAAAATTGCAATGCAGTTTCGTGTTTCACCCTTAAAGTTATAAAATGCATGCTTgtgttcgac                                                                                                                       >  2:213583/1‑133 (MQ=255)
           tttAATAAATCCTGCAGCCTCTTGATTATCAATCTCCGGTCCCTGCTCTAAAATTTCTGTTTGTTGCCCTTCATGTAAAATTGCAATGCAGTTTCGTGTTTCACCCTTAATGTTATAAAATGCATGCTTGATGTCGGCATg                                                                                                                    >  1:810035/1‑141 (MQ=255)
             taataaCTCCAGCAGCCTCTTGATTATCAATCTCCGGTCCCTGCTCTAAAATTTCTGTTTGTTGCCCTTCATGTAAAATTGCAATGCAGTTTCGTGTTTCACCCTTAATGTTATAAAATGCATGCTTGATGTCGGCATGAt                                                                                                                  <  1:547353/141‑1 (MQ=255)
              aataaATCCTGCAGCCTCTTGATTATCAATCTCCGGTCCCTGCTCTAAAATTTCTGTTTGTTGCCCTTCATGTAAAATTGCAATGCAGTTTCGTGTTTCACCCTTAATGTTGTAAAATGCATGCTTGATGTCgcaatgttc                                                                                                                 >  2:700347/1‑133 (MQ=255)
              aataaATCCTGCAGCCTCTTGATTATCAATCTCCGGTCCCTGCTCTAAAATTTCTGTTTGTTGCCCTTCATGTAAAATTGCAATGCAGTTTCGTGTTTCACCCTTAATGTTGTAAAATGCATGCTTGATGTCGACATgtaa                                                                                                                 >  2:232359/1‑138 (MQ=255)
              aataaATCCTGCAGCCTCTTGATTATCAATCTCCGGTCCCTGCTCTAAAATTTCTGTTTGTTGCCCTTCATGTAAAATTGCAATGCAGTTTCGTGTTTCACCCTTAATGTTATAAAATGCATGTTTGATGTCGGAATgttt                                                                                                                 >  2:462630/1‑138 (MQ=255)
              aataaATCCTGCAGCCTCTTGATTATCAATCTCCGGTCCCTGCTCTAAAATTTCTGTTTGTTGCCCTTCATGTAAAATTGCAATGCAGTTTCGTGTTTCACCCTTAATGTTATAAAATGCATGCTTGATGTCGGCATGATc                                                                                                                 <  1:122655/141‑1 (MQ=255)
               atcaaTCCTCCAGCCTCGTGATTATCCATCTCCGGTCCCTGCTCTAAAATTTCTGTTTGTTGCCCTTCATGTAAAATTGCAATGCAGTTTCGTGTTTCACCCTTAATGTTATAAAATGCATGCTTGATGTCGGCATGATCt                                                                                                                <  1:103651/138‑1 (MQ=255)
               ataaATCCTGCAGCCTCTTGATTATCAATCTCCGGTCCCTGCTCTAAAATTTCTGTTTGTTGCCCTTCATGTAAAATTGCAATGCAGTTTCGTGTTTCACCCTTAATGTTATAAAATGCATGCTTGATGTCGGCATGATCt                                                                                                                <  1:596683/141‑1 (MQ=255)
               ataaATCCTGCAGCCTCTTGATTATCAATCTCCGGTCCCTGCTCTAAAATTTCTGTTTGTTGCCCTTCATGTAAAATTGCAATGCAGTTTCGTGTTTCACCCTTAATGTTATAAAATGCATGCTTGATGTCGGCATGATCt                                                                                                                >  2:699190/1‑141 (MQ=255)
                         cAGCCTCTGGATTATCAATCTCCGGTCCCTGCTCTAAAATTTCTGTTTGTTGCCCTTCATGTAAAATTGCAATGCAGTTTCGTGTTTCACCCTTAATGTTATAAAATGCATGCTTGATGTCGGCATGATCTAATTTTTTAg                                                                                                      <  2:883776/141‑1 (MQ=255)
                          aGCCTCTTGATTATCAATCTCCGGTCCCTGCTCTAAAATTTCTGTTTGTTGCCCTTCATGTAAAATTGCAATACAGTTTCGTGTTTCACCCTTAATGTTATAAAATGCATGCTTGATGTCGGCATGATCTAATTTTTTAGc                                                                                                     >  2:671545/1‑141 (MQ=255)
                           gCCTCTTGATTATCAATCTCCGGTCCCTGCTCTAAAATTTCTGTTTGTTGCCCTTCATGTAAAATTGCAATGCAGTTTCGTGTTTCACCCTTAATGTTATAAAATGCATGCTTGATGTCGGCATGATCTAATTTTTTAGCa                                                                                                    >  2:456472/1‑141 (MQ=255)
                            gctctTGATTATCAATCTCCGGTCCCTGCTCTAGAAGTTCTGTTTGTTGCCCTTCACGTAAAATTGCAATGCAGTTTCGTGTTTCACCCTTACTCTTATAAAATGCATGCTTGATGTCGGCATGATCTACTTTTTTACCat                                                                                                   >  2:24163/2‑140 (MQ=255)
                            ccTCTTTATTATCAATCTCCGGTCCCTGCTCTAAAATTTCTGTTCGTTGCCCTTCATGTAAAATTGCAATGCAGTTTCGTGTTTCACCCTTAATGTTATAAAATGCATGCTTTATGTCGGCATGATCTAATTTTTTAGCaa                                                                                                   <  2:482992/141‑1 (MQ=255)
                            ccTCTTGATTTTCAATCTTCGGTCCCTGCTCTAAAATTTCTGTTTGTTGCCCTTCATGTAAAATTGCAATGCAGTTTCGTGTTTCCACTTTAATGTTATATAATGCATGCTTGATGTCGGCATGATCTAATTTTTTAGCaa                                                                                                   <  2:757616/141‑1 (MQ=255)
                            ccTCTTGATTATCAATCTCCGGTCCCTGCTCTAAAATTTCTGTTTGTTGCCCTTCATGTAAAATTGCAATGCAGTTTCGTGTTTCACCCTTAATGTTATAAAATGCATGCTTGATGTCGGCATGATCTAATTTTTTAGCaa                                                                                                   <  1:102766/141‑1 (MQ=255)
                            ccTCTTGATTATCAATCTCCGGTCCCTGCTCTAAAATTTCTGTTTGTTGCCCTTCATGTAAAATTGCAATGCAGTTTCGTGTTTCACCCTTAATGTTATAAAATGCATGCTTGATGTCGGCATGATCTAATTTTTTAGCaa                                                                                                   <  1:837307/141‑1 (MQ=255)
                             ctctTGATTATCAATCTCCGGTCCCTGCTCTAAAATTTCTGTTTGTTGCCCTTCCTGTAACATTGCAATGCAGTTTCGTGTTTCACCCTTAATGTTATAAAATGCATGCTTGATGTCGGCATGATCTAATTTTTAGCAATa                                                                                                 >  1:450562/1‑141 (MQ=255)
                              tctTGATTATCAATCTCCGGTCCCTGCTCTAAAATTTCTGTTTGTTGCCCTTCATGTAAAATTGCAATGCAGTTTCGTGTTTCACCCTTAATGTTATAAAATGCATGCTTGATGTCGGCATGATCTAATTTTTTAGCAATa                                                                                                 <  1:356669/141‑1 (MQ=255)
                                  gATTATCAATCCCCGGTCCCTGCTCTAAAATTTCTGTTTGTTGCCCTTCATGTAAAATTGCAATGCAGTTTCGTGTTTCACCCTTAATGTTATAAAATGCATGCTTGATGTCGGCATGATCTAATTTTTTAGCAATAAAtt                                                                                             <  2:61863/141‑1 (MQ=255)
                                   tttATCAATCTCCGGTCCCTGCTCTAAAATTTCTGTTTGTTGCCCCTCATGTAAAATTGCAATGCAGGTTCGTGTTTCACCCTTAATGTTATAAAATGCATGCTTGATGTCGGCATGATCTAATTTTTTAGCAATAAATTg                                                                                            <  2:354873/140‑1 (MQ=255)
                                   aTTATCAATCTCCGGTCCCTGCTCTAAAATTTCTGTTTGTTGCCCTTCATGTAAAATTGCAATGCAGTTTCGTGTTTCACCCTTAATGTTATAAAATGCATGCTTGATGTCGGCATGATCTAATTTTTTAGCAATAAATTg                                                                                            <  2:202522/141‑1 (MQ=255)
                                    ttATCAATCTCCGGTCCCTGCTCTAAAATTTCGGTTTGTTGCCCTTCATGTAAAATTGCAATGCAGTTTCGTGTTTCACCCTTAATGTTATAAAATGCATGCTTGATGTTGGCATGGTCTAATTTTTTGGCAATAATTTgc                                                                                           >  1:86268/1‑140 (MQ=255)
                                     tATCAATCTCCGGTCCCTGCTCTAAAATTTCTGTTTGTTGCCCTTCATGTAAAATTTGAATGCAGTTTCGTGTTTCACCCTTAATGTTATAAAATGCATGCTTGATGCCGTCATGATCTAATTTTTTAGCAATAAATTGAc                                                                                          <  2:237929/141‑1 (MQ=255)
                                     tATCAATCTCCGGTCCCTGCTCTAAAATTTCTGTTTGTTGCCCTTCATGTAAAATTGCAATGCAGTTTCGTGTTTCACCCTTAATGTTATAAAATGCATGCTTGATGTCGGCATGATCTAATTTTTTAGCAATAAATTGAc                                                                                          >  2:264310/1‑141 (MQ=255)
                                         aaTCTCCGGTCCCTGCTCTAAAATTTCTGTTTGTTGCCCTTCATGTAAAATTGCAATGCAGTTTCGTGTTTCACCCTTAATGTTATAAAATGCATGCTTGATGTCGGCATGATCTAATTTTTTAGCAATAAATTGACCTaa                                                                                      <  2:375345/141‑1 (MQ=255)
                                          aTCTCCGGTCCCTGCTCTAAAATTTCTGTTTGTTGCCCTTCATGTAAAATTGCAATGCAGTTTCGTGTTTCACCCTTAATGTTATAAAATGCATGCTTGATGTCGGCATGATCTAATTTTTTAGCAATAAATGGACCTagt                                                                                     >  2:37106/1‑139 (MQ=255)
                                          aTCTCCGGGCCCTGCTCTAAAATTTCTGTTTGTTGCCCTTCATGTAAAATTGCAATGCAGTTTCGTGTTTCACCCTTAATGTTATACAATGCATGCTTGATGTCGGCATGATCTAATTCTTTAGCAATAAATTGACCTAAt                                                                                     >  2:695881/1‑141 (MQ=255)
                                             tcCGGTCCCTGCTCTAAAATTTCTGTTTATTGCCCTTCATGTAAAATTGCAATGCAGTTTCGTGTTTCACCCTTAGTGTTATAAAATGCATGCTTGATTTCGGCATGATCCACTTTTTTAGCCATATATTGACCTACTTCa                                                                                  >  2:934136/1‑141 (MQ=255)
                                              ccGGTCCCTGCTCTAAAATTTCTGTTTGTTGCCCTTCATGTAAAATTGCAATGCAGTTTCGTGTTTCACCCTTAATGTTATAAAATGCATGCTTGATGTCGGCATGATCTAATTTTTTAGCAATCAATTGACCTAATTCAc                                                                                 >  2:445370/1‑141 (MQ=255)
                                              ccGGTCCCTGCTCTAAAATTTCTGTTTGTTGCCCTTCATGTAAAATTGCAATGCAGTTTCGTGTTTCACCCGTAATGTTATAAAATGCATGCTTGATGTCGGCATGATCTAATTTTTTAGCAATAAATTGACCTAATTCAc                                                                                 <  1:220176/141‑1 (MQ=255)
                                                ggTCCCTGTTCTAGAATTTCTGTTCGTTGCCCTTCATGTAAAACTGCAACGCAGTTTCGTAGTTCCTCCGTTATGGTATATCATGCATGGTTGATGTTGCCCTGATCTAATTTTTTCGCAATAAATTGACCTAATTCACCg                                                                               <  2:126738/141‑1 (MQ=255)
                                                ggTCCCTGCTCTAAAATTTCTGTTTGTTGCCCTTCATGTAAAATTGCAATGCAGTTTCGTGTTTCACCCTTAATGTTATAAAATGCATGCTTGATGTCGGCATGATCTAATTTTTTAGCAATAAATTGACCTAATTCACCg                                                                               >  1:512673/1‑141 (MQ=255)
                                                ggTCCCTGCTCTAAAATTTCTGTTTGTTGCCCTTCATGTAAAATTGCAATGCAGTTTCGTGTTTCACCCTTAATGTAATAAAATGCATGCTGGATGTCGGCTAGGTCTAATTTTTTAGCAATAAATTGACCTAATTCACCg                                                                               <  1:932251/141‑1 (MQ=255)
                                                 gTCCCTGCTCTAAAATTTCTGTTTGTTGCCCTTCATGTAAAATTGCAATGCAGTTTCGTGTTTCACCCTTAATGTTATAAAATGCATGCTTGATGTCGGCATGATCTAATTTTTTAGCAATAAATTGGCCTAATTCACCGc                                                                              >  2:678630/1‑141 (MQ=255)
                                                 gTCCCTGCTCTAAAATTTCTGTTTGTTGCCCTTCATGTAAAATTGCAATGCAGTTTCGTGTTTCACCCTTAATGTTATAAAATGCATGCTTGATGTCGGCATGATCTAATTTTTTAGCAATAAATTGACCTAATTCACCGc                                                                              >  1:840705/1‑141 (MQ=255)
                                                 gTCCCTGCTCTAAAATTTCTGTTTGTTGCCCTTCATGTAAAATTGCAATGCAGTTTCGTGTTTCACCCTTAATGTTATAAAATGCATGCTTGATGTCGGCATGATATAATTTTTTAGTATTAAATTGACCTAATTCAgcgc                                                                              >  1:18911/1‑141 (MQ=255)
                                                  tCCCTGCTCTAAAATTTCTGTTTGTTGCCCTTCATGTAAAATTGCAATGCAGTTTAGTGTTTCACCCTTAATGTTATAAAATGCATGCTTGATGTCGGCATGATCTAATTTTTTAGCAATAAATTGACCTAATTCACCGcc                                                                             >  2:124654/1‑141 (MQ=255)
                                                   cccTGCTCTAAAATTTCTGTTTGTTGCCCTTCATGTAAAATTGCAATGCAGTTTCGTGTTTCACCCTTAATGTTATAAAATGCATGCTTGATGTCGGCATGATCTAATTTTTTAGCAATAAATTGACCTAATTCACCGCCa                                                                            >  1:176993/1‑141 (MQ=255)
                                                   cccTGCTCTAAAATTTCTGTTTGTTGCCCTTCATGTAAAATTGCAATGCAGTTTCGTGTTTCACCCTTAATGTTATAAAATGCATGCTTGATGTCGGCATGATCTAATTTTTTAGCAATAAATTGACCTAATTCACCGCCa                                                                            >  1:82649/1‑141 (MQ=255)
                                                    ccTGCTCTAAAATTTCTGTTTGTTGCCCTTCATGTAAAATTGCAATGCAGTTTCGTGTTTCACCCTTAATGTTATAAAATGCATGCTTGATGTCGGCATGATCTAATTTTTTAGCAATAAATTGACCTAATTCACCGCCaa                                                                           >  1:153727/1‑141 (MQ=255)
                                                       gCTCTAAAATTTCTGTTTGTTGCCCTTCATGTAAAATTGCAATGCAGTTTCGTGTTTCACCCTTAATGTTATAAAATGCATGCTTGATGTCGGCATGATCTAATTTTTTAGCAATAAATTGACCTAATTCACCGCCAATaa                                                                        <  2:895877/141‑1 (MQ=255)
                                                        gtctAAAATTTCTGTTTCTTGCCCTTCATGTAAAATTGCAATGCAGTTTCGTGTTTCACCCTTAATGTTATAAAATGCATGCTTGATGTCGGCATGATCTAATTTTTTAGCAATAAATTGACCTAATTCACCGCCAATaaa                                                                       <  2:166724/140‑1 (MQ=255)
                                                        ctctAAAATTTCTGTTTGTTGCCCTTCATGTAAAATTGCAATGCAGTTTCGTGTTTCACCCTTAATGTTATAAAATGCATGCTTGATGTCGGCATGATCTAATTTTTTAGCAATAAATTGACCTAATTCACCGCCAATaaa                                                                       <  1:210954/141‑1 (MQ=255)
                                                           tAAAATTTCTGTTTGTTGCCCTTCATGTAAAATTGCAATGCAGTTTCGTGTTTCACCCTTAATGTTATAAAATGCATGCTTGATGTCGGCATGATCTAATTTTTTAGCAATAAATTGACCTCATTCACCGCCAATAAAAcc                                                                    <  1:564226/141‑1 (MQ=255)
                                                           tAAAATTTCTGTTTGTTGCCCTTCATGTAAAATTGCAATGCAGTTTCGTGTTTCACCCTTAATGTTATAAAATGCATGCTTGATGTCGGCATGATCTAATTTTTTAGCAATAAATTGACCTAATTCACCGCCAATAAAAcc                                                                    <  2:866889/141‑1 (MQ=255)
                                                             aaaTTTCTGTTTGTTGCCCTTCATGTAAAATTGCAATGCAGTTTCGTGTTTCACCCTTAATGTTATAAAATGCATGCTTGATGTCGGCATGATCTAATTTTTTAGCAATAAATTGACCTAATTCACCGCCAATAAAACCAc                                                                  >  1:511102/1‑141 (MQ=255)
                                                                   cTGTTTGTTGCCCTTCATGTAAAATTGCAATGCAGTTTCGTGTTTCACCCTTAATGTTATAAAATGCATGCTTGATGTCGGCATGATCTAATTTTTTAGCAATAAATTGACCTAATTCACCGCCAATAAAACCACTCGCaa                                                            >  1:698181/1‑141 (MQ=255)
                                                                       ttgttgCCCTTCATGTAAAATTGCAATGCAGTTTCGTGTTTCACCCTTAATGTTATAAAATGCATGCTTGATGTCGGCATGATCTAATTTTTTAGCAATAAATTGACCTAATTCACCGCCAATAAAACCACTCGCAAGGAc                                                        <  2:368245/141‑1 (MQ=255)
                                                                                ttgaTGTAAAATTGCAATGCAGTTTCGTGTTTCACCCTTAATGTTATAAAATGCATGCTTGATGTCGGCATGATCTAATTTTTTAGCAATAAATTGACCTAATTCACCGCCAATAAAACCACTCGCAAGGACTGGCTCAcc                                               <  1:877232/138‑1 (MQ=255)
                                                                                 tCATGTAAAATTGCAATGCAGTTTCGTGTTTCACCCTTAATGTTATAAAATGCATGCTTGATGTCGGCATGATCTAATTTTTTAGCAATAAATTGACCTAATTCACCGCCAATAAAACCACTCGCAAGGACTGGCTCACCt                                              <  1:250179/141‑1 (MQ=255)
                                                                                 tCATGTAAAATTGCAATGCAGTTTCGTGTTTCACCCTTAATGTTATAAAATGCATGCTTGATGTCGGCATGATCTAATTTTTTAGCAATAAATTGACCTAATTCACCGCCAATAAAACCACTCGCAAGGACTGGCTCACCt                                              <  1:408243/141‑1 (MQ=255)
                                                                                   ataaaagaagacAATGCAGTTTCGTGTTTCACCCTTAATGTTATAAAATGCATGCTTGATGTCGGCAGGATCTAATTTTTTAGCAATAAATTGACCTAATTCACCGCCACTAAAACGACTCCCGAGGAATAGCTCAccgcc                                            >  2:344844/12‑138 (MQ=255)
                                                                                     gTAAAATTGCAATGCAGTTTCGTGTTTCACCCTTAATGTTATAAAATGCATGCTTGATGTCGGCATGATCTAATTTTTTAGCAATAAATTGACCTAATTCACCGCCAATAAAACCACTCGCAAGGACTGGCTCACCTACtt                                          >  1:792144/1‑141 (MQ=255)
                                                                                      tAAAATTGCAATGCAGTTTCGTGTTTCACCCTTAATGTTATAAAATGCATGCTTGATGTCGGCCTGATCTAATTTTTTAGCAATAAATTGACCTAATTCACCGCCAATAAAACCACTCGCAAGGACTGGCTCACCTACTTg                                         >  2:739474/1‑141 (MQ=255)
                                                                                      tAAAATTGCAATGCAGTTTCGTGTTTCACCCTTAATGTTATAAAATGCATGCTTGATGTCGGCATGATCTAATTTTTTAGCAATAAATTGACCTAATTCACCACCAATAAAACCACTCGCAAGGACTGGCTCACCTCCttc                                         >  2:427180/1‑140 (MQ=255)
                                                                                        aaaTTGCACTGCAGTTTCGTGTTTGACCCTTATTGTTATAAAATGCATGCTTGATGTCGGCATGATCTAATTTTTTAGCAATAAATTGACCTAATTCACCGCCAATAAAACCACTCGCAAGGACTGGCTCACCTACTTgcg                                       <  1:18599/141‑1 (MQ=255)
                                                                                        aaaTTGCAATGCAGTTTCGTGTTTCACCCTTAATGTTATAAAATGCATGCTTGATGTCGGCATGATCTAATTTTTTAGCAATAAATTGACCTAATTCACCGCCAATAAAACCTCTCGCAAGGACTGGCTCACCTACTTgcg                                       <  1:273810/141‑1 (MQ=255)
                                                                                        aaaGTGCAATGCAGTTTCGTGTTTCACCCTTAATGTTATAAAATGCATGCTTGATGTCGGCATGATCTAATTTTTTAGCAATAAATTGACCTAATTCACCGCCAATAAAACCACTCGCAAGGACTGGCTCACCTACTTgcg                                       <  1:434758/141‑1 (MQ=255)
                                                                                         aaTTGCAATGCAGTTTCGTGTTTCACCCTTAATGTTATAAAATGCATGCTTGATGTCGGCATGATCTAATTTTTTAGCAATAAATTGACCTAATTCACCGCCAATAAAACCACTCGCAAGGCCTGGCTCACCTACTTgcgc                                      >  1:755022/1‑141 (MQ=255)
                                                                                           ttGCAATGCAGTTTCGTGTTTCACCCTTAATGTTGTAAAATGCATGCTTGATGTCGGCATGATCTAATTTTTTAGCAATAAATTGACCTAATTCACCGCCAATAAAACCACTCGCAAGGACTGGCTCACCTACTTGCGCaa                                    <  1:700347/141‑1 (MQ=255)
                                                                                           ttGCAATGCAGTTTCGTGTTTCACCCTTAATGTTGTAAAATGCATGCTTGATGTCGGCATGATCTAATTTTTTAGCAATAAATTGACCTAATTCACCGCCAATAAAACCACTCGCAAGGACTGGCTCACCTACTTGCGCaa                                    <  1:232359/141‑1 (MQ=255)
                                                                                           ttGCAATGCAGTTTCGTGTTTCACCCTTAATGTTATAAAATGCATGCTTGATGTCGGCATGATCTAATTTTTTAGCAATAAATTGACCTAATTCACCGCCAATAAAACCACTCGCAAGGACTGGCTCACCTACTTGCGCaa                                    <  1:462630/141‑1 (MQ=255)
                                                                                            tGCAATGCAGTTTCGTGTTTCACCCTTAATGTTATAAAATGCATGCTTGATGTCGGCATGATCTAATTTTTTAGCAATAAATTGACCTAATTCACCGCCAATAAAACCACTCGCAAGGACTGGCCAACCTACTTGCGCAAg                                   >  1:367789/1‑141 (MQ=255)
                                                                                              cAATGCAGTTTCGTGTTTCACCCTTAATGTTATAAAATGCATGCTTGATGTCGGCATGATCTAATTTTTTTTTTTTTTTTTTACCTAATTCACCGCAAATAAAACCACTCGCAGGGACTGGCTCACCTACTTGCGCAAGtc                                 >  2:166781/1‑140 (MQ=255)
                                                                                               aaTGCAGTTTCGTGTTTCACCCTTAATGTTATAAAATGCATGCTTGATGTCGGCATGATCTAATTTTTTAGCAATAAATTGACCTAATTCACCGCCAATAAAACCACTCGCAAGGACTGGCTCACCTACTTGCGCAAGTAc                                >  1:597060/1‑141 (MQ=255)
                                                                                                  gCAGTTTCGTGTTTCACCCTTAATGTTATAAAATGCATGCTTGATGTCGGCATGATCTAATTTTTTAGCAATAAATTGACCTAATTCACCGCCAATAAAACCACTCGCAAGGACTGGCTCACCTACTTGCGCAAGTActct                             <  2:511102/141‑1 (MQ=255)
                                                                                                  gCAGTTTCGTGTTTCACCCTTAATGTTATAAAATGCATGCTTGATGTCGGCATGATCTAATTTTTTAGCAATAAATTGACCTAATTCACCGCCAATAAAACCACTCGCAAGGACTGGCTAGCCTACTTGCGCAAGTAcact                             >  1:320636/1‑141 (MQ=255)
                                                                                                    aGTTTCGTGTTTCACCCTTAATGTTATAAAATGCATGCTTGATGTCGGCATGATCTAATTTTTTAGCAATAAATTGACCTAATTCACCGCCAATAAAACCACTCGCAAGGACTGGCTCACCTACTTGCGCAAGTACTCTTg                           <  2:840705/141‑1 (MQ=255)
                                                                                                    aGTTTCGTGTTTCACCCTTAATGTTATAAAATGCATGCTTGATGTCGGCATGATCTAATTTTTTAGCAATAAATTGACCTAATTAACCGCCAATAAAACCACCCGCAAGGACTGGCTCCCCTACTGGCGCAAGTACTCttt                           >  2:104126/1‑140 (MQ=255)
                                                                                                     gTTTCGTGTTTCACCCTTAATGTTATAAAATGCATGCTTGATGTCGGCATGATCTAATTTTTTAGCAATAAATTGACCTAATTCACCGCCAATAAAACCACTCGCAAGGACTGGATCACCTACCTGCGGAAGTACTCTTGt                          >  1:826195/1‑141 (MQ=255)
                                                                                                       ttCGTGTTTCACCCTTAATGTTATAAAATGCATGCTTGATGTCGGCATGATCTAATTTTTTAGCAATAAATTGACCTAATTCACCGCCAATAAAACCACTCGCAAGAACAGGTAATACGGCGTGCGCAAGTACTCTTGTTa                        <  1:456472/141‑1 (MQ=255)
                                                                                                        tCGTGTTTCACCCTTAATGTTATAAAATGCATGCTTGATGTCGGCATGATCTAATTTTTTAGCAATAAATTGACCTAATTCACCGCCAATAAAACCACTCGCAAGGACTGGCTCACCTACTTGCGCAAGTACTCTTGTTAc                       >  1:652540/1‑141 (MQ=255)
                                                                                                          ggggttCAGCTTTTATGTTGTAAAATGGAGGCTTGATGTAGGCATGATCTAATTTTTTAGCAATAAATTGACCTAATGCACCGCCAATAAAACCACTCGCATGGAGTGGCTCACCTACTTGCGCAAGTACTGTTGTTACAt                     <  2:442472/137‑1 (MQ=255)
                                                                                                           gttttCACCCTTAATGTTATAAAATGCATGCTTGATGTCGGCATGATCTAATTTTTTAGCAATAAATTGACCTAATTCACCGCCAATAAAACCACTCGCAAGGACTGGCTCACCTACTTGCGCAAGTACTCTTGTTACAtt                    <  2:835129/139‑1 (MQ=255)
                                                                                                             tttCACCCTTAATGTTATAAAATGCATGCTTGATGTCGGCATGATCTAATTTTTTAGCAATAAATTGACCTAATTCACCGCCAATAAAACCACTCGGAAGGACTGGCTCACCTACTTGCGCAAGTACTCTTGTTACATTTa                  <  2:501827/141‑1 (MQ=255)
                                                                                                                   ccTTAATGTTATAAAATGCATGCTTGATGTCGGCATGATCTAATTTTTTAGCAATAAATTGACCTAATTCACCGCCAATAAAACCACTCGCAAGGACTGGCTCACCTACTTGCGCAAGTACTCTTGTTACATTTAAACCtt            <  1:866326/141‑1 (MQ=255)
                                                                                                                      tAATGTTATAAAATGCATGCTTGATGTCGGCATGATCTAATTTTTTAGCAATAAATTGACCTAATTCACCGCCAATAAAACCACTCGCAAGGACTGGCTCACCTACTTGCGCAAGTACTCTTGTTACATTTAAACCTTTac         >  1:70051/1‑141 (MQ=255)
                                                                                                                        aTGTTATGAAATTCATTCTTGATTTCGTCTTGGTCTAATTTTTTAGCAATAAATTGACCTAATTCACCGCCAATAAAACCACTCGCAAGGACTGGCTCACCTACTTGCGCAAGTACTCTTGTTACATTTAAACCTTTacca       >  2:789962/1‑141 (MQ=255)
                                                                                                                        aTGTTATAAAATGCATGCTTGATGTCGGCATGATCTAATTTTTTAGCAATAAATTGACCTAATTCACCGCCAATAAAACCACTCGCAAGGACTGGCTCACCTACTTGCGCAAGTACTCTTGTTACATTTAAACCTTTacca       >  2:676349/1‑141 (MQ=255)
                                                                                                                        aTGTTATAAAATGCATGCTTGATGTCGGCATGATCTAATTTTTTAGCAATAAATTGACCTAATTCACCGCCAATAAAACCACTCGCAAGGACTGGCTCACCTACTTGCGCAAGTACTCTTGTTACATTTAAACCTTTacca       >  2:432195/1‑141 (MQ=255)
                                                                                                                          gTTATAAAATGCATGCTTGATGTCGGCATGATCTAATTTTTTAGCAATAAATTGACCTAATTCACCGCCAATAAAACCACTCGCAAGGACTGGCTCACCTACTTGCGCAAGTACTCTTGTTACATTTAAACCTTTaccacc     >  1:154141/1‑141 (MQ=255)
                                                                                                                            tataAAATGCATGCTTGATGTCGGCATGATCTAATTTTTTAGCAATAAATTGACCTAATTCACCGCCAATAAAACCACTCGCAAGGACTGGCTCACCTACTTGCGCAAGTACTCTTGTTACATTTAAACCTTTACCACCAg   <  2:810035/141‑1 (MQ=255)
                                                                                                                             ataAAATGCATGCTTGATGTCGGCATGATCTAATTTTTTAGCAATAAATTGACCTAATTCACCGCCAATAAAACCACTCGCAAGGACTGGCTCACCTACTTGCGCAAGTACTCTTGTTACATTTAAACCTTTACCACCAGc  >  1:367828/1‑141 (MQ=255)
                                                                                                                             ataAAATGCATGCTTGATGTCGGCATGATCTAATTTTTTAGCAATAAATTGACCTAATTCACCGCCAATAAAACCACTCGCAAGGACTGGCTCACCTACTTGCGCAAGTACTCTTGTTACATTTAAACCTTTACCACCAGc  >  1:798313/1‑141 (MQ=255)
                                                                                                                             |                                                                                                                                            
TGTTCAAAATGTTTAATAAATCCTGCAGCCTCTTGATTATCAATCTCCGGTCCCTGCTCTAAAATTTCTGTTTGTTGCCCTTCATGTAAAATTGCAATGCAGTTTCGTGTTTCACCCTTAATGTTATAAAATGCATGCTTGATGTCGGCATGATCTAATTTTTTAGCAATAAATTGACCTAATTCACCGCCAATAAAACCACTCGCAAGGACTGGCTCACCTACTTGCGCAAGTACTCTTGTTACATTTAAACCTTTACCACCAGC  >  CP000730/2331551‑2331816

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: