Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,540,782 G→A 7.4% E118K (GAA→AAA)  USA300HOU_2401 → immunoglobulin G‑binding protein SBI

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,540,7820GA 7.4% 318.5 / 2.7 116E118K (GAA→AAA) USA300HOU_2401immunoglobulin G‑binding protein SBI
Reads supporting (aligned to +/- strand):  ref base G (52/52);  new base A (2/8);  total (55/61)
Fisher's exact test for biased strand distribution p-value = 9.87e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 116 total aligned reads displayed.

TACATCAAAACATTACGCGAACACCCAGAACGTGCACAAGAAGTATTCTCTGAATCACTTAAAGACAGCAAGAACCCAGACCGACGTGTTGCACAACAAAACGCTTTTTACAATGTTCTTAAAAATGATAACTTAACTGAACAAGAAAAAAATAATTACATTGCACAAATTAAAGAAAACCCTGATAGAAGCCAACAAGTTTGGGTAGAATCAGTACAATCTTCTAAAGCTAAAGAACGTCAAAATATTGAAAATGCGGATAAAGCAATTAAAGA  >  CP000730/2540644‑2540918
                                                                                                                                          |                                                                                                                                        
   aTCAAAACATTACGCGAACACCCAGAACGTGCACAAGAAGTATTCTCTGAATCACTTAAAGACAGCAAGAACCCAGACCGACGTGTTGCACAACAAAACGCTTTTTACAATGTTCTTAAAAATGATAACTTAACTGAACaa                                                                                                                                     <  1:78923/141‑1 (MQ=255)
   aTCAAAACATTACGCGAACACCCAGAACGTGCACAAGAAGTATTCTCTGAATCACTTAAAGACAGCAAGAACCCAGACCGACGTGTTGCACAACAAAACGCTTTTTACAATGTTCTTAAAAATGATAACTTAACTGAACaa                                                                                                                                     <  2:11915/141‑1 (MQ=255)
    tCAAAACATTACGCGAACCCCCAGAACGCGCACAAGAAGTATTCTCCGAATCACTTAAAGACAGCAAGAACCCAAACCGACGTGTTGCACAACAAAACCCAATAAAAAAAGACCTTAAAAAAGATAACATAACCAAACaaa                                                                                                                                    >  2:911917/1‑140 (MQ=255)
     ctaaaactgaCGCGAACACCCAGAACGTGCACAAGAAGTATTCTCTGAATCACTTAAAGACAGCAAGAACCCAGACGTCCGTGTTGCTCAACAAAACGCTTTTTACAATGTTCGTAAAGTTGGTTACTTAACTTAACAAGa                                                                                                                                   <  2:61321/132‑1 (MQ=255)
        aaCATTACGCGAACACCCAGAACGTGCACAAGAAGTATTCTCTGAATCACTTAAAGACAGCAAGAACCCAGACCGACGTGTTGCACAACAAAACGCTTTTTACAATGTTCTTAAAAATGATAACTTAACTGAACAAGaaaa                                                                                                                                <  1:401729/141‑1 (MQ=255)
        aaCATTACCCGAACACCCAGAACGTGCACAAGAAGTATTCTCTGAATCACTTAAAGACAGCAAGAACCCAGACCGACGTGTTGCACAACAAAACGCTTTTTACAATGTTCTTAAAAATGATAACTTAACTGAACAAGaaaa                                                                                                                                <  1:360712/141‑1 (MQ=255)
        aaCATGACGCGAACACCCAGACCCCACACAAGAGGTATTCGCTGAATCACTTAAAGACACCAAGAACCCAGGCCGACGTGTTGCACAACAAACCGCTTTTTACAATGTTCTTAAAAATGATAAATTAACTGAACAAGaaaa                                                                                                                                <  2:194484/141‑1 (MQ=255)
          cATTACGCGAACACCCAGAACGTGCACAAGAAGTATTCTCTGAATCACTTAAAGACAGCAAGAACCCAGACCGACGTGTTGCACAACAAAACGCTTTTTACAATGTTCTTAAAAATGATAACTTAACTGAACAAGaaaaaa                                                                                                                              <  2:608351/141‑1 (MQ=255)
          cATTACGCGAACACCCAGAACGTGCACAAGAAGTATTCTCTGAATCACTTAAAGACAGCAAGAACCCAGACCGACGTGTTGCACAACAAAACGCTTTTTACAATGTTCTTAAAAATGATAACTTAACTGAACAAGaaaaaa                                                                                                                              >  1:50815/1‑141 (MQ=255)
           agtACGCGAACACCCAGACCGTGCACAAGAAGTATTCTCTGAATCACTTAAAGACAGCAAGAACCCAGACCGACGTGTTGCACAACAAAACGCTTTTTACAATGTTCTTAAAAATGATAACTTAACTGAACAAGaaaaaaa                                                                                                                             <  1:517771/139‑1 (MQ=255)
           aTTACGCGAACACCCAGAACGTGCACAAGAAGTATTCTCTGAATCACTTAAAGACAGCAAGAACCCAGACCGACGTGTTGCACAACAAAACGCTTTTTACAATGTTCTTAAAAATGATAACTTAACTGAACAAGaaaaaaa                                                                                                                             <  1:360877/141‑1 (MQ=255)
             tACGCGAACACCCAGAACGTGCACAAGAAGTATTCTCTGAATCACTTAAAGACAGCAAGAACCCAGACCGACGTGTTGCACAACAAAACGCTTTTTACAATGTTCTTAAAAATGATAACTTAACTGAACAAGAAAAAaata                                                                                                                           >  1:597906/1‑141 (MQ=255)
               cgcgAACCTCCAGAACGTGCACAAGAAGTATTCTCTGAATCACTTAAAGACAGCAACACCCCAGACCTACGTGTTGCACAACAAAACGCTTTTTACAATGTTCTTAAAAATGATAACTTAACTGAACAAGAAAAAaataat                                                                                                                         <  1:938273/141‑1 (MQ=255)
                 cgAACACCCAGAACGTGCACAAGAAGTATTCTCTGAAGCACTTAAAGACAGCAAGAACCCAGACCGACGTGTTGCACAACAAAACGCTTTTTACAATGTTCTTAAAAATGATAACTTAACTGAACAAGAAAAAAATAATTa                                                                                                                       <  1:583890/141‑1 (MQ=255)
                    acacCCAGAACGTGCACAAGAAGTATTCTCTGAATCACTTAAAGACAGCAAGAACCCAGACCGACGTGTTGCACAACAAAACGCTTTTTACAATGTTCTTAAAAATGATAACTTAACTGAACAAGAAAAAAATAATTACAt                                                                                                                    <  1:120463/141‑1 (MQ=255)
                    acacCCAGAACGTGCACAAGAAGTATTCTCTGAATCACTTAAAGACAGCAAGAACCCAGACCGACGTGTTGCACAACAAAACGCTTTTTACAATGTTCTTAAAAATGATAACTTAACTGAACAAGAAAAAAATAATTACAt                                                                                                                    >  2:298503/1‑141 (MQ=255)
                    acacCCAGAACGTGCACAAGAAGTATTCTCTGAATCACTTAAAGACAGCAAGAACCCAGACCGACGTGTTGCACAACAAAACGCTTTTTACAATGTTCTTAAAAATGATAACTTAACTGAACAAGAAAAAAATAATTACAt                                                                                                                    <  1:756270/141‑1 (MQ=255)
                      acCCAGAACGTGCACAAGAAGTATTCTCTGAATCACTTAAAGACAGCAAGAACCCAGACCGACGTGTTGCACAACAAAACGCTTTTTACACTGTTCTTAAAAATGATAACTTAACTGAACAAGAAAAAAATAATTACATTg                                                                                                                  >  1:424281/1‑141 (MQ=255)
                       cccAGAACGTGCACAAGAAGTATTCTCTGAATCACTTAAAGACAGCAACAACCCAGACCGACGTGTTGCACAACAAAACGCTTTTTACAATGTTCTTAAAAATGATAACTTAACTGAACAAAAAAAAAATAATTACaatac                                                                                                                 >  2:721822/1‑137 (MQ=255)
                       cccAGAACGTGCACAAGAAGTAATCTCTGAAGCACTAAAAGACAGCCAAAACCAAGCCCAACATGATAAACAAAAAAACGCTAACAACAATGCAAAAAAAAACGATAAATTAACTGAACAAGAAAAAACTAATAACaataa                                                                                                                 >  2:261321/1‑137 (MQ=255)
                        ccAGAACGTGCACAAGAAGTATTCTCTGAATCACTTAAAGACAGCAAGAACCCAGACCGACGTGTTGCACAACAAAACGCTTTTTACAATGTTCTTAAAAATGATAACTTAACTGAACAAGAAAAAAATAATTACATTGca                                                                                                                <  1:745060/141‑1 (MQ=255)
                        ccAGAACGTGCACAAGAAGTATTCTCTGAATCACTTAAAGACAGCAAGAACCCAGACCGACGTGTTGCACAACAAAACGCTTTTTACAATGTTCTTAAAAATGATAACTTAACGGAACAAGAAAAAAATAATTACATTGca                                                                                                                >  1:163878/1‑141 (MQ=255)
                          aGAACGTGCACAAGAAGTATTCTCTGAATCACTTAAAGACAGCAAGAACCCAGACCGACGTGTTGCACAACAAAACGCTTTTTAAAATGTTCTTAAAAATGATAACTTAACTGAACAAGAAAAAAATAATTACATTGcaca                                                                                                              <  2:192115/141‑1 (MQ=255)
                            aaCGTGCACAAGAAGTATTCTCTGAATCACTTAAAGACAGCAAGAACCCAGACCGACGTGTTGCACAACAAAACGCTTTTTACAATGTTCTTAAAAATGATAACTTAACTGAACAAGAAAAAAATAATTACATTGCACaaa                                                                                                            >  2:269422/1‑141 (MQ=255)
                             acctGCACAAGAAGTATTCTCTGAATCACTTAAAGACAGCAAGAACCCAGACCGACGTGTTGCACAACAAAACGCTTTTTACAATGTTCTTAAAAATGATAACTTAACTGAACAAGAAAAAAATAATTACATTGCACAAAt                                                                                                           <  1:16981/138‑1 (MQ=255)
                             aCGTGCACAAGAAGTATTCTCTGAATCACTTAAAGACAGCAAGAACCCAGACCGACGTGTTGCACAACAAAACGCTTTTTACAATGTTCTTAAAAATGATAACTTAACTGAACAAGAAAAAAATAATTACATTGCACAAAt                                                                                                           <  1:206320/141‑1 (MQ=255)
                                 gCACAAGAAGTATTCTCTGAATCACTTAAAGACAGCAAGAACCCAGACCGACGTGTTGCACAACAAAACGCTTTTTACAATGTTCTTAAAAATGATAACTTAACTGAACAAGAAAAAAATAATTACATTGCACAAATTaaa                                                                                                       >  1:332450/1‑141 (MQ=255)
                                   cacagaagTATTCTCTGAATCACTTAAAGACAGCAAGAACCCAGACCGACGTGTTGCACAACAAAACGCTTTTTACAATGTTCTTAAAAATGATAACTTAACTGAACAAGAAAAAAATAATTACATTGCACAAATTAAAGa                                                                                                     <  1:344314/138‑1 (MQ=255)
                                   acaAGAAGTATTCTCTGAATCACTTAAAGACAGCAAGAACCCAGACCGACGTGTTGCACAACAAAACGCTTTTTACAATGTTCTTAAAAATGATAACTTAACTGAACAAGAAAAAAATAATTACATTGCACAAATTAAAGa                                                                                                     <  2:699194/141‑1 (MQ=255)
                                   acaAGAAGTATTCTCTAAATCAATTAAAGACAGCAAGAACCCAGACCGACGTGTTGCACAACAAAACGCTATTTACAATGTTCTTAAACCTGATAACTTAACAAAAAAAAAAAAAAATAATTACATTGCACAAATTAAAGa                                                                                                     <  2:319706/141‑1 (MQ=255)
                                        aattATTCTCTGAATCACTTAAAGACAGCAAGAACCCAGAGCGACGTGTTGCACAACAAAACGCTTTTTACAATGTTCTTAAAAATGATAACTTAACCGAACAAGAAAAAAATAATTACATTGCACAAATTAAAGAAAAcc                                                                                                <  2:388043/138‑1 (MQ=255)
                                        aagAATTCTATAAAACACTTAAAGACAGAAAGAACCCAGACCGACGTGTTGCACAACAAAACGCTATTTACAATGTTCTTAAAAATGATAACTTAAAAAAAAAAAAAAAAAATAATTACATTGCACAAATTAAAGAAAAcc                                                                                                <  2:131032/141‑1 (MQ=255)
                                         agTATTCTCTGAATCACTTAAAGACAGCAAGAACCCAGACCGACGTGTTGCACAACAAAACGCTTTTTACAATGTTCTTAAAAATGATAACTTAACTGAACAAGAAAAAAATAATTACATTGCACAAATTAAAGAAAAccc                                                                                               >  1:651102/1‑141 (MQ=255)
                                         agTATTCTCTGAATCACTTAAAGACAGCAAGAACCCAGACCGACGTGTTGCACAACAAAACGCTTTTTACAATGTTCTTAAAAATGATAACTTAACTGAACAAGAAAAAAATAATTACATTGCACAAATTAAAGAAAAccc                                                                                               >  2:560967/1‑141 (MQ=255)
                                          gTATTCTCGGAATCACTTAAAGACCGCAAGCACCCAGCCCGACGTGTTGCATAACAAAACGCTTTTTACAATGTTCTTAAAAATGATAACTTAACTGAACAAGAAAAACATAATTACATTGCACAAATTAAAGAAAACCCt                                                                                              <  2:504504/141‑1 (MQ=255)
                                            aTTCTCTGAATCACTTAAAGACAGCAAGAACCCAGACCGACGTGTTGCACAACAAAACGCTTTTTACAATGTTCTTAAAAATGATAACTTAACTGAACAAGAAAAAAATAATTACATTGCACAAATTAAAGAAAACCCCGa                                                                                            >  1:277109/1‑141 (MQ=255)
                                                tctGAATCACTTAAAGACAGCAAGAACCCAGACCGACGTGTTGCACAACAAAACGCTTTTTACAATGTTCTTAAAAATGATAACTTAACTGAACAAGAAAAAAATAATTACATTGCACAAATTAAAGAAAACCCTGATAGa                                                                                        >  1:669913/1‑141 (MQ=255)
                                                 ctGAATCACTTAAAGACAGCAAGAACCCAGACCGACGTGTTGCACAACAAAACGCTTATTACCATGTTCTTAAAAATGATACCTTAACTGAACAACAAAAAAATAATTACATTGCACAAATTAAAGAAAAACCTAATaaaa                                                                                       >  2:133916/1‑141 (MQ=255)
                                                    aagcatggaaaGACAGCAAGAACCCAGAGCGACGTGGTGCACAACAAAACGCTTTTTACAATGTTCTTAAAAATGATAACTTAACTGAACAAGAAAAAAATAATGACATTGGACAAATTAAAGAAAACCCTGATAGAAGcc                                                                                    <  2:129914/133‑1 (MQ=255)
                                                      gcACTTAAAGGCAGCGAGAACCGAGACCCACGTGTTGCACCACAAAACGCAATTTAGAATGTTCTTAAACATGATAACTTAAAAAAAAAAAAAAAAAATAATTACATTGCACAAATTAAAGAAAACCCTGATAGAAGCcaa                                                                                  <  2:559326/140‑1 (MQ=255)
                                                      agaCTTAAAGACAGCAAGAACCCCGACACACGTGTTGCACCACAAAACGCTTTTTACAATGTTCTTAAAAATGATAACTCAACTGAACAAGAAAAAAATAATTACCATGCCGTTTATAAAGAATACCCTGATAGAAGCcaa                                                                                  <  1:456006/139‑1 (MQ=255)
                                                      acaatgaaaGACAACAAGAACCCAGACCGACGTCAGGCACAAAAAACCGCTTTTTACAATATTCTTAAAAATGATAACAAAACAAAAAAAAAAAAAAATAATTACATTGCACAAATTAAAGAAAACCCTGATAGAAGCcaa                                                                                  <  2:383720/135‑1 (MQ=255)
                                                       cacaaaaaGACAGCAAGAACCCAGACCGACGTGTTGCACAACAAAACGCTTTTTATAATGTTCTTAAAAATGATAACTTAACAAAAAAAAAAAAAAATAATTACATTGCACAAATTAAAGAAAACCCTGATAGAAGCcaac                                                                                 <  2:892133/136‑1 (MQ=255)
                                                         cTTAAAGACAGCAAGAACCCAGACCGACGTGTTGCACAACAAAACGCTTTTTACAATGTTCTTAAAAATGATAACTTAACTGAACAAGAAAAAAATAATTACATTGCACAAATTAAAAAAAACCCTGATAGAAGCcaacaa                                                                               >  1:74414/1‑141 (MQ=255)
                                                             aataaaGCAAGAAGCCAGCCCGACGTGTTGCACAACAAAACGCTTTTTACAATGTTCTTAAAAATGATAACTTAACTGAACAAGAAAAAAATAATTACATTGCACAAATTAAAGAAAACCCTGATAGAAGCCAACAAGttt                                                                           <  2:563048/136‑1 (MQ=255)
                                                             aaGACAGGAAGAACCCAGACCGACGTGTTGCACAACAAGACGCTTTTTACAATGGTCTTAAAAATGATAACTTAACTGAACAAGAAAAAAATAATTACATTGCACAAATTAAAGAAAACCCTGATAGAAGCCAACAAGttt                                                                           <  2:41374/141‑1 (MQ=255)
                                                             aaGACAGCAAGAACCCAGACCGACGTGTTGCACAACACAACGCTTTTTACAATGTTCTTAAAAATGATAACTTAACTGAACAAGAAAAAAATAATTACATTGCACAAATTAAAGAAAACCCTGATAGAAGCCAACAAGttt                                                                           <  1:848709/141‑1 (MQ=255)
                                                             aaGACAGCAAGAACCCAGACCGACGTGTTGCACAACAAAACGCTTTTTACAATGTTCTTAAAAATGATAACTTAACTGAACAAGAAAAAAATAATTACATTGCACAAATTAAAGAAAACCCTGATAGAAGCCAACAAGttt                                                                           <  1:794447/141‑1 (MQ=255)
                                                              agccAGCAAGAACCCAGACCGACGTGCTGCACAACAAAACGCTTTTTACAATGTTCTTACAAATGATAACTTAACTTAACAAGACAAAAATAATTACATTGCACAACTTAAAGAAAACCCTGCTAGAAGCCAACAAGTTTg                                                                          >  2:916952/4‑141 (MQ=255)
                                                              aGACAGCAAGAACCCAGACCGACGTGTTGCACAACAAAACGCTTTTTACAATGTTCTTAAAAATGATAACTTAACTGAACAAGAAAAAAATAATTACATTGCACAAATTAAAGAAAACCCTGATAGAAGCCAACAAATTTg                                                                          >  2:713487/1‑141 (MQ=255)
                                                                aCAGCAAGAACCCAGACCGACGTGTTGCACAACAAAACGCTTTTTACAATGCTATACAATCGTATAACTTAACTGAACAAGAAAAAAATAATTACATTGCACAAATTAAAGAAAACCCTGATAGAAGCCAACAAGTTTggg                                                                        <  1:448015/141‑1 (MQ=255)
                                                                  aGCAAGAACCCAGACCGACGTGTTGCACAACAAAACGCTTTTTACAATGTTCTTAAAAATGATAACTTAACTGAACAAGAAAAAAATAATTACATTGCACAAATTAAAGAAAACCCTGATAGAAGCCAACAAACTTGGGTa                                                                      >  1:531170/1‑141 (MQ=255)
                                                                   gCAAGAACCCAGACCGACGTGTTGCACAACAAAACGCTTTTTACAATGTTCTTAAAAATGATAACTTAACTGAACAAGAAAAAAATAATTACATTGCACAAATTAAAGAAAACCCTGATAGAAGCCAACAAGTTTGGGTAg                                                                     >  1:325571/1‑141 (MQ=255)
                                                                   gCAAGAACCCAGACCGACGTGTTGCACAACAAAACGCTTTTTACAATGTTCTTAAAAATGATAACTTAACTGAACAAGAAAAAAATAATTACATTGCACAAATTAAAGAAAACCCTGATAGAAGCCAACAAGTTTGGGTAg                                                                     >  2:318051/1‑141 (MQ=255)
                                                                     aaGAACCCAGACCGACGTGTTGCACAACAAAACGCTTTTTACAATGTTCTTAAAAATGATAACTTAACTGAACAAGAAAAAAATAATTACATTGCACGAATTAAAGAAAACCCTGATAGAAGCCAACAAGTTTGGGTAGaa                                                                   >  1:812784/1‑141 (MQ=255)
                                                                        agcccAGACCGACGTGTTGCACAACAAAACGCTTTTTACAATGTTCTTAAAAATGATAACTTAACTGAACAAGAAAAAAATAATTACATTGCACAAATTAAAGAAAACCCTGATAGAAGCCAACAAGTTTGGGTAGAATCa                                                                >  2:650706/3‑141 (MQ=255)
                                                                        aaCCCAGACCGACGTGTTGCACAACAAAACGCTTTTTACAATGTTCTTAAAAATGATAACTTAACTGAACAAGAAAAAAATAATTACATTGCACAAATTAAAGAAAACCCTGATAGAAGCCAACAAGTTTGGGTAGAATCa                                                                >  2:683252/1‑141 (MQ=255)
                                                                         aCCCAGACCGACGTGTTGCACAACAAAACGCTTTTTACAATGTTCTTAAAAATGATAACTTAACTGAACAAGAAAAAAATAATTACATTGCACAAATTATAGAAAACCCTGATAGAAGCCAACAAGTTTGGGTAGAATCAg                                                               <  1:647430/141‑1 (MQ=255)
                                                                          tgcaaactgACGTGTTGCACAACAAAACGCTTTTTACAATGTTCTTAAAAATGATAACTTAACTGAACAAGAAAAAAATAATTACATTGCACAAATTAAAGAAAACCCTGATAGAAGCCAACAAGTTTGGGTAGAATCAGt                                                              <  2:231805/133‑1 (MQ=255)
                                                                          cccAGACCGACGTGTTGCACAACAAAACGCTTTTTACAATGTTCTTAAAAATGATAACTTAACTGAACAAGAAAAAAATAATTACATTGCACAAATTAAAGAAAACCCTGATAGAAGCCAACAAGTTTGGGTAGAATCAGt                                                              <  2:47403/141‑1 (MQ=255)
                                                                             aGACCGACGTGTTGCACAACAAAACGCTTTTTACAATGTTCTTAAAAATGATAACTTAACTGAACAAGAAAAAAATAATTACATTGCACAAATTAAAGAAAACCCTGATAGAAGCCAACAAGTTTGGGTAGAATCAGTACa                                                           >  1:730029/1‑141 (MQ=255)
                                                                             aGACCGACGTGTTGCACAACAAAACGCTTTTTACAATGTTCTTAAAAATGATAACTTAACTGAACAAGAAAAAAATAATTACATTGCACAAATTAAAGAAAACCCTGATAGAAGCCAACAAGTTTGGGTAGAATCAGTACa                                                           >  1:480392/1‑141 (MQ=255)
                                                                                ccGACGTGTTGCACAACAAGACGCTTTTTACAATGTTCTTAAAAATGATAACTTAACTGAACAAGAAAAAAATAATTACATTGCACAAATTAAAGAAAACCCTGATAGAAGCCAACAAGTTTGGGTAGAATCAGTACAAtc                                                        >  1:518791/1‑141 (MQ=255)
                                                                                ccGACGTGTTGCACAACAAAACGCTTTTTACAATGTTCTTAAAAATGATAACTTAACTGAACAAGAAAAAAATAATTACATTGTATTATGTAAAGAAAACCCTGATAGAAGCCAACAAGTTTGGGTAGAATCAGTACAAtc                                                        >  1:731359/1‑141 (MQ=255)
                                                                                ccGACGTGTTGCACAACAAAACGCTTTTTACAATGTTCTTAAAAATGATAACTTAACTGAACAAGAAAAAAATAATTACATTGCACAAATTAAAGAAAACCCTGATAGAAGCCAACAAGTTTGGGTAGAATCGGTACAAtc                                                        >  1:270146/1‑141 (MQ=255)
                                                                                ccGACGTGTTGCACAACAAAACGCTTTTTACAATGTTCTTAAAAATGATAACTTAACTGAACAAGAAAAAAATAATTACATTGCACAAATTAAAGAAAACCCTGATAGAAGCCAACAAGTTTGGGTAGAATCAGTACAAtc                                                        >  1:877473/1‑141 (MQ=255)
                                                                                 caaCGTGGTGCACAACAAAAACAATTCTACAATGTTCATAAAAATGACAACTTAAAAAAAAAAAAAAAAAATAATTACATTGCACAAATTAAAGAAAACCCTGATAGAAGCCAACAAGTTTGGGTAGAATCAGTACAAtct                                                       <  2:824904/139‑1 (MQ=255)
                                                                                   acctgtgGCACAACAAAACGCTTTTTACAATGTGCTTAAAAATGATAACTTAACTGAACAAGAAAAAAATAATTACATTGCACAAATTAAAGAAAACCCTGATAGAAGCCAACAAGTTTGGGTAGAATCAGTACAAtcttc                                                     <  1:659348/138‑1 (MQ=255)
                                                                                    cGTGTTGCACAACAAAACGCTTTTTACAATGTTCTTAAAAATGATAACTTAACTGAACAAGAAAAAAATAATTACATTGCACAAATTAAAGAAAACCCTGATAGAAGCCAACAAGTTTGGGTAGAATCAGTACAAtcttct                                                    >  2:864585/1‑141 (MQ=255)
                                                                                          gCACAACAAAACGCTTTTTACAATGTTCTTAAAAATGATAACTTAACTGAACAAGAAAAAAATAATTACATTGCACAAATTAAAGAAAACCCTGAGAGAAGCCAACAAGTTTGGGTAGAATCAGTACAATCTTctaaagct                                              >  1:776305/1‑141 (MQ=255)
                                                                                          gCACAACAAAACGCTTTTTACAATGTTCTTAAAAATGATAACTTAACTGAACAAGAAAAAAATAATTACATTGCACAAATTAAAGAAAACCCTGAGAGAAGCCAACAAGTTTGGGTAGAATCAGTACAATCTTctaaagct                                              >  1:776394/1‑141 (MQ=255)
                                                                                            acaacaAAACGCTTTTTACAATGTTCTTAAAAATGATAACTTAACTGAACAAGAAAAAAATAATTACATTGCACAAATTAAAGAAAACCCTGATAGAAGCCAACAAGTTTGGGTAGAATCAGTACAATCTTctaaagctaa                                            >  2:831709/1‑141 (MQ=255)
                                                                                               acaaAACGCTTTTTACAATGTTCTTAAAAATGATAACTTAACTGAACAAGAAAAAAATAATTACATTGCACAAATTAAAGAAAACCCTGATAGAAGCCAACAAGTTTGGGTAGAATCAGTACAATCTTCTAAAGCTAAAGa                                         >  1:422507/1‑141 (MQ=255)
                                                                                               acaaAACGCTTTTTACAATGTTCTTAAAAATGATAACTTAACTGAACAAGAAAAAAATAATTACATTGCACAAATTAAAGAAAACCCTGATAGAAGCCAACAAGTTTGGGTAGAATCAGTACAATCTTCTAAAGCTAAAGa                                         >  1:773373/1‑141 (MQ=255)
                                                                                                  aaaCGCTTTTTACAATGTTCTTAAAAATGATAACTTAACTGAACAAGAAAAAAATAATTACATTGCACAAATTAAAGAAAACCCTGATAGAAGCCAACAAGTTTGGGTAGAATCAGTACAATCTTCTAAAGCTAAAGAACg                                      <  1:542369/141‑1 (MQ=255)
                                                                                                   aaCGCTTTTTACAATGTTCTTAAAAATGATAACTTAACTGAACAAGAAAAAAATAATTACATTGCACAAATTAAAGAAAACCCTGATAGAAGCCAACAAGTTTGGGTAGAATCAGTACAATCTTCTAAAGCTAAAGAACGt                                     <  2:146608/141‑1 (MQ=255)
                                                                                                   aaCGCTTTTAACAATGTTCTTACAAATGATAACTTAACTGAACAAGAAAAAAATAATTACATTGCACAAATTAAAGAAAACCCTGATAGAAGCCAACAAGTTTGGGTAGAATCAGTACAATCTTCTAAAGCTAAAGAACGt                                     <  2:753019/141‑1 (MQ=255)
                                                                                                    aCGCTTTTTACAATGTTCTTAAAAATGATAACTTAACTGAACAAGAAAAAAATAATTACATTGCACAAATTAAAGAAAACCCTGATAGAAGCCAACAAGTTTGGGTAGAATCAGTACAATCTTCTAAAGCTAAAGAACGTc                                    >  2:20362/1‑141 (MQ=255)
                                                                                                           ttACAATGTTCTTAAACATGCTAACTTAACTGAACAAGAAAAAAATAATTACATTGCACAAATTAAAGAAAACCCTGATAGAAGCCAACAAGTTTGGGTAGAATCAGTACAATCTTCTAAAGCTAAAGAACGTCAAAatat                             >  1:936887/1‑141 (MQ=255)
                                                                                                             aCAATGTTCTTAAAAATGATAACTTAACTGAACAAGAAAAAAATAATTACATTGCACAAATTAAAGAAAACCCTGATAGAAGCCAACAAGTTTGGGTAGAATCAGTACAATCTTCTAAAGCTAAAGAACGTCAAAATATTg                           <  1:318051/141‑1 (MQ=255)
                                                                                                             aCAATGTTCTTAAAAATGATAACTTAACTGAACAAGAAAAAAATAATTACATTGCACAAATTAAAGAAAACCCTGATAGAAGCCAACAAGTTTGGGTAGAATCAGTACAATCTTCTAAAGCTAAAGAACGTCAAAATATTg                           <  2:332450/141‑1 (MQ=255)
                                                                                                             aCAATGTTCTTAAAAATGATAACTCAACTGAACAAGAAAAAAATAATTACATTGCACAAATTAAAGAAAACCCTGATAGAAGCCAACAAGTTTGGGTAGAATCAGTACAATCTTCTAAAGCTAAAGAACGTCAAAATATTg                           <  1:250398/141‑1 (MQ=255)
                                                                                                                 tGTTCTTAAAAATGATAACTTAACTGAACAAGAAAAAAATAATTACATTGCACAAATTAAAGAAAACCCTGATAGAAGCCAACAAGTTTGGGTAGAATCAGTACAATCTTCTAAAGCTAAAGAACGTCAAAATATTGaaaa                       >  1:920669/1‑141 (MQ=255)
                                                                                                                    tCTTAAAAATGATAACTTAACTGAACAAGAAAAAAATAATTACATTGCACAAATTAAAGAAAACCCTGATAGAAGCCAACAAGTTTGGTTAGAATCAGTACAATCTTCTAAAGCTAAAGAACGTCAAAATATTGAAAATGc                    <  1:410662/141‑1 (MQ=255)
                                                                                                                    tCTTAAAAATGATAACTTAACTGAACAAGAAAAAAATAATTACATTGCACAAATTAAAGAAAACCCTGATAGAAGCCAACAAGTTTGGGTAGAATCAGTACAATCTTCTAAAGCTAAAGAACGTCATAATATTGAAAATGc                    <  1:503206/141‑1 (MQ=255)
                                                                                                                          aaaTGATAACTTAACTGAACAAGAAAAAAATAATTACATTGCACAAATTAAAGAAAACCCTGATAGAAGCCAACAAGTTTTGGTAGAATCAGTACAATCTTCTAAAGCTAAAGAACGTCAAAATATTGAAAATGCGGATaa              >  1:247801/1‑141 (MQ=255)
                                                                                                                           aaTTATAACTTAACTGAACAAGAAAAAAATAATTACATTGCACAAATTAAAGAAAACCCTGATAGAAGCCAACAAGTTTGGGTAGAATCAGTACAATCTTCTAAAGCTAAAGAACGTCAAAATATTGAAAATGCGGATaaa             <  1:874872/141‑1 (MQ=255)
                                                                                                                           aaTGATAACTTAACTGAACAAGAAAAAAATAATTACATTGCACAAATTAAAGAAAACCCTGATAGAAGCCAACAAGTTTGGGTAGAATCAGTACAATCTTCTAAAGCTAAAGAACGTCAAAATATTGAAAATGCGGATaaa             <  1:298503/141‑1 (MQ=255)
                                                                                                                           aaTGATAACTTAACTGAACAAGAAAAAAATAATTACATTGCACAAATTAAAGAAAACCCTGATAGAAGCCAACAAGTTTGGGTAGAATCAGTACAATCTTCTAAAGCTAAAGAACGTCAAAATATTGAAAATGCGGATaaa             >  2:175819/1‑141 (MQ=255)
                                                                                                                           aaTGATAACTTAACTGAACAAGAAAAAAATAATTACATTGCACAAATTAAAGAAAACCCTGATAGAAGCCAACAAGTTTGGGTAGAATCAGTACAATCTTCTAAAGCTAAAGAACGTCAAAATATTGAAAATGCGGATaaa             <  2:459081/141‑1 (MQ=255)
                                                                                                                           aaTGATAACTTAACTGAACAAGAAAAAAATAATTACATTGCACAAATTAAAGAAAACCCTGATAGAAGCCAACAAGTTGGGGTAGAATCAGTACAATCTTCTAAAGCTAAAGAACGTCAAAGTATTGAAAATGCGGATaaa             >  1:271600/1‑141 (MQ=255)
                                                                                                                           aaTGATAACTTAACTGAACAAGAAAAAAATAATTACATTGCACAAATTAAAGAAAACACTGATAAAAGCCAACAAGTTTGGGTAGAATCAGTACAATCTTCTAAAGCTAAGGAACGTCAAAATATTGAAAATACGGATaaa             >  1:501292/1‑141 (MQ=255)
                                                                                                                           aaTGATAACTTAACTGAACAAGAAAAAAATAATTAAATTGCAAAAATTAAAGAAAACCCTGATAGAAGCCAACAAGTTTGGGTAGAATCAGTACAATCTTCTAAAGCTAAAGAAAGTCAAAATATTGAAAATGCGGATaaa             >  2:172611/1‑141 (MQ=255)
                                                                                                                           aaTGATAACTTAACTGAAAAAGAAAAAAATAATTACATTGCACAAATTAAAGAAAACCATGATAGAAGCCAACAAGTTTGGTTGGAGTAAGTACAATCTTCAAAAGCTAAAGAACGTCAAAATATTGAAAATCCGCATaaa             >  2:375270/1‑141 (MQ=255)
                                                                                                                              gATAACTTAACTGAACAAGAAAAAAATAATTACATTGCACAAATTAAAGAAAACCCTGATAGAAGCCAACAAGTTTGGGTAGAATCAGTACAATCTTCTAAAGCTAAAGAACGTCAAAATATTGAAAATGCGGATAAAGCa          >  2:465590/1‑141 (MQ=255)
                                                                                                                                aaacttaactGAACAAGAAAAAAATAATTACATTGCACAAATTAAAGAAAACCCTGATAGAAGCCAACAAGTTTGGGTAGAATCAGTACAATCTTCTAAAGCTAAAGAACGTCAAAATATTGAAAATGCGGATAAAGCAAt        <  1:398202/140‑1 (MQ=255)
                                                                                                                                 aacttaactGAACAAGAAAAAAATAATTACATTGCACAAATTAAAGAAAACCCTGATAGAAGCCAACAAGTTTGGGTAGAATCAGTACAATCTTCTAAAGCTAAAGAACGTCAAAATATTGAAAATGCGGATAAAGCAAtt       <  1:144787/141‑1 (MQ=255)
                                                                                                                                  acttaactGAACAAGAAAAAAATAATTACATTGCACAAATTAAAGAAAACCCTGATAGAAGCCAACAAGTTTGGGTAGAATCAGTACAATCTTCTAAAGCTAAAGAACGTCAAAATATTGAAAATGCGGAACAAGCAATTa      >  2:205992/1‑141 (MQ=255)
                                                                                                                                  acttaactGAACAAGAAAAAAATAATTACATTGCACAAATTAAAGAAAACCCTGATAGAAGCCAACAAGTTTGGGTAGAATCAGTACAATCTTCTAAAGCTAAAGAACGTCAAAATATTGAAAATGCGGAAAAAGCAATTa      >  2:274436/1‑141 (MQ=255)
                                                                                                                                  acctaactGAACAAGAAAAAAATAATTACCTTGCACAAATTAAAGAAAACCCTGATAGAAGCCAACAAGTTTGGGTAGAATCAGTACAATCTTCTAAAGCTAAAGAACGTCAAAATATTGAAAATGCGGATAAAGCAATTa      <  1:754958/138‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                        
TACATCAAAACATTACGCGAACACCCAGAACGTGCACAAGAAGTATTCTCTGAATCACTTAAAGACAGCAAGAACCCAGACCGACGTGTTGCACAACAAAACGCTTTTTACAATGTTCTTAAAAATGATAACTTAACTGAACAAGAAAAAAATAATTACATTGCACAAATTAAAGAAAACCCTGATAGAAGCCAACAAGTTTGGGTAGAATCAGTACAATCTTCTAAAGCTAAAGAACGTCAAAATATTGAAAATGCGGATAAAGCAATTAAAGA  >  CP000730/2540644‑2540918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: