Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	2,771,843	C→A	9.0%	F214L (TTC→TTA)	phoB →	alkaline phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,771,843	0	C	A	9.0%	264.0 / 3.3	87	F214L (TTC→TTA)	phoB	alkaline phosphatase
Reads supporting (aligned to +/- strand):  ref base C (20/56);  new base A (3/7);  total (24/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGACTTCTAGAGATGATAAAAACGAAATCGCTAAACAATTTTATAAAGATAAAATCAACGGAAAGCATAAAGTTGATGTTTTATTAGGCGGCGGCGCTAAATATTTCGGTAAAAGTAATGGCAATTTAGATAAAAAATTCAAAAAAGATGGTTACGACCTTGCCACGAACAGTAAGGAGTTATCAAAATCTGATAAAGATAAAGTTCTTGGTTTGTTTGCAGATAAAAATATGCCACTTGCAATAGATGCTTCTAAAGACGAACCATCATTAGCT  >  CP000730/2771703‑2771978                                                                                                                                             |                                                                                                                                        gTGACTTCTAGAGATGATAAAAACGAAATCGCTAAACAATTTTATAAAGATAAAATCAACGGAAAGCATAAAGTTGATGTTTTATTAGGCGGCGGCGCTAAATATTTCGGTAAAAGTAATGGCAATTTAGATAAAAAATTc                                                                                                                                         >  1:7686/1‑141 (MQ=255) gTGACTTCTAGAGATGATAAAAACGAAATCGCTAAACAATTTTATAAAGATAAAATCAACGGAAAGCATAAAGTTGATGTTTTATTAGGCAGCGGCGCTAAATATTTCGGTAAAAGTAATGGCAATTTAGATAAAAAATTc                                                                                                                                         >  2:645449/1‑141 (MQ=255)       tCTAGAGATGATAAAAACGAAATCGCTAAACAATTTTATAAAGATAAAATCAACGGAAAGCATAAAGTTGATGTTTTATTAGGCGGCGGCGCTAAATATTTCGGTAAAAGTAATGGCAATTTAGATAAAAAATTCaaaaaa                                                                                                                                   <  1:470328/141‑1 (MQ=255)       tCTAGAGAGGATAAAAACGAAATCGCTAAACAATTTTAGAAAGATAAAATCAACGGAAAGCATAAAGTTGATGTTTTATTAGGCGGCGGCGCTAAATATTTCGGTAAAAGTAATGGCAATTTAGATAAAAAATTCaaaaaa                                                                                                                                   <  1:77263/141‑1 (MQ=255)       tCTAGAGAGAATAAAAACCAAATCGCTAAACAATTTTATAAAGATAAAATCAACGGAAAGCATAAAGTTGATGTTTTATTAGGCGGCGGCGCTAAATATTTCGGTAAAAGTAATGGCAATTTAGATAAAAAATTCaaaaaa                                                                                                                                   <  1:483346/141‑1 (MQ=255)         tAGAGATGATAAGAACGAAATCGCTAAAAAAATTTATAAAGATAAAATGGACGGAAAGCATAAAGTGGGTGTTTTATTAGGCGGCGGCGCTAAATATTTCGGTAGAAGGAGTGGCTATGTTGAAAAAAAAAAAAAAAAAGa                                                                                                                                 <  2:508066/141‑1 (MQ=255)            agaTGATAAAAACGAAATCGCTAAACAATTTTATAAAGATAAAATCAACGGAAAGCATAAAGTTGATGTTTTATTAGGCGGCGGCGCTAAATATTTCGGTAAAAGTAATGGTAATTTAGATAAAAAATTCAAAAAAGATgg                                                                                                                              <  1:654600/141‑1 (MQ=255)             gatgatAAAAACGAAATCGCTAAACAATTTTATAAAGATAAAATCAACGGAAAGCATAAAGTTGATGTTTTATTAGGCGGCGGCGCTAAATATTTCGGTAAAAGTAATGGCAATTTAGATAAAAAATTCAAAAAAGATGGt                                                                                                                             >  2:78964/1‑141 (MQ=255)             gatgatAAAAACGAAATCGCTAAACAATTTTATAAAGATAAAATCAACGGAAAGCATAAAGTTGATGTTTTATTAGGCGGCGGCGCTAAATATTTCGGTAAAAGTAATGGCAATTTAGATAAAAAATTCAAAAAAGATGGt                                                                                                                             >  2:843091/1‑141 (MQ=255)                gaaaaaaaCGAAATCGATAAACCATTTTATAAAGATAAAAACAACGGGAAGCATTAAGTTGATGTTTTATGAGGCGGCGGCGCTAAATATTTCACTAAAAGTAGTGGCAATTTATATAAAAAATTCAAAAAAGATGGTTAc                                                                                                                          <  2:610910/138‑1 (MQ=255)                   aaaaaCGAAATCGCTAAACAATTTTATAAAGATAAAATCAACGGAAAGCATAAAGTTGATGTTTTATTAGGCGGCGGCGCTAAATATTTCGGTAAAAGTAATGGCAATTTAGATAAAAAATTCAAAAAAGATGGTTACGAc                                                                                                                       <  1:239578/141‑1 (MQ=255)                    aaaaCGAAATCGCTAAACAATTTTATAAAGATAAAATCAACGGAAAGCATAAAGTTGATGTTTTATTAGGCGGCGGCGCTAAATATTTCGGTAAAAGTAATGGCAATTTAGATAAAAAATTCAAAAAAGATGGTTACGAcc                                                                                                                      >  1:103737/1‑141 (MQ=255)                      aaCGAAATCGCTAAACAATTTTATAAAGATAAAATCAACGGAAAGCAGAAAGTTGATGTTTTATTAGGCGGCGGCGCTAAATATTTCGGTAAAAGTAATGGCAATTTAGATAAAAAATTCAAAAAAGATGGTTACGACCtt                                                                                                                    >  1:485846/1‑141 (MQ=255)                       aCGAAATCGCTAAACAATTTTATAAAGATAAAATCAACGGAAAGCATAAAGTTGATGTTTTATTAAGCGGCGGCGCTAAATATTTCGGTAAAAGTAATGACAATTTAGATAAAAAATGCAAAAAAGATGGTTCAAACCTTg                                                                                                                   >  2:306974/1‑141 (MQ=255)                        cGAAATCGCTAAACAATTTTATAAAGATAAAATCAACGGAAAGCATAAAGTTGATGTTTTATTAGGCGGCGGCGCTAAATATTTCGGTAAAAGTAATGGCAATTTAGATAAAAAATTCAAAAAAGATGGTTACGACCTTGc                                                                                                                  <  1:580604/141‑1 (MQ=255)                        cGAAATCGCTAAACAATTTTATAAAGATAAAATCAACGGAAAGCATAAAGTTGATGTTTTATTAGGCGGCGGCGCTAAATATTTCGGTAAAAGTAATGGCAATTTAGATAAAAAATTCAAAAAAGATGGTTACGACCTTGc                                                                                                                  <  2:764243/141‑1 (MQ=255)                        cGAAATCGCTAAACAATTTTATAAAGATAAAATCAACGGAAAGCATAAAGTTGATGTTTTATTAGGCGGCGGCGCTAAATATTTCGGTAAAAGTAATGGCAATTTAGATAAAAAATTCAAAAAAGATGGTTACGACCTTGc                                                                                                                  <  1:374948/141‑1 (MQ=255)                        cGAAAGCGCTAAACGATTTTATAAAGATAAAATCAACGGAAAGCATAAAGTTGATGTTTTATTAGGCGGCGGCGCTAAATATTTCGGTAAAAGTAATGGCAATTTAGATAAAAAATTCAAAAAAGATGGTTACGACCTTGc                                                                                                                  <  2:457825/141‑1 (MQ=255)                         gAAATCTCTATACAATTTTATAAAGATAAAATCAACGGAAAGCATAAAGTTGATGTTTTATTAGGCGGCGGCGCTAAATATTTCGGTAAAAGTAATGGCAATTTAGATAAAAAATTCAAAAAAGATGGTTACGACCTTGcc                                                                                                                 <  2:192193/141‑1 (MQ=255)                         gAAATCGCTAAACAATTTTATAAAGATAAAATCAACGGAAAGCATAAAGTTGATGATTTATTAGGCGGCGGCGCTAAATATTTCGGTAAAAGTAATGGCAATTTAGATAAAAATGGTGAAAAAGATGGACATGACCTTGcc                                                                                                                 >  2:947019/1‑141 (MQ=255)                          aaaTCGCTAAACAATTTTATAAAGATAAAATCAACGGAAAGCATAAAGTTGATGTTTTATTAGGCGGCGGCGCTAAATATTTCGGTAAAAGTAATGGCAATTTAGATAAAAAATTCAAAAAAGATGGTTACGACCTTGCCa                                                                                                                >  2:938614/1‑141 (MQ=255)                           aaTCGCTAAACAATTTTATAAAGATAAAATCAACGGAAAGCATAAAGTTGATGTTTTATTAGGCGGCGGCGCTAAATATTTCGGTAAAAGTAATGGCAATTTAGATAAAAAATTCAAAAAAGATGGTTACGACCTTGCCAc                                                                                                               >  2:740711/1‑141 (MQ=255)                           aaTCGCTAAACAATTTTATAAAGATAAAATCAACGGAAAGCATAAAGTTGATGTTTTATTAGGCGGCGGCGCTAAATATTTCGGTAAAAGTAATGGCAATTTAGATAAAAAATTAAAAAAAGATGGTTACGACCTTGCCAc                                                                                                               >  2:266339/1‑141 (MQ=255)                            aTCGCTAAACAATTTTATAAAGATAAAATCAACGGAAAGCATAAAGTTGATGTTTTATTAGGCGGCGGCGCTAAATATTTCGGTAAAAGTAATGGCAATTTAGATAAAAAATTCAAAAAAGATGGTTACGACCTTGCCACg                                                                                                              <  1:644633/141‑1 (MQ=255)                               gCTAAACAATTTTATAAAGATAAAATCAACGGAAAGCATAAAGTTGATGTTTTATTAGGCGGCGGCGCTAAATATTTCGGTAAAAGTAATGGCAATTTAGATAAAAAATTCAAAAAAGATGGTTACGACCTTGCCACGAAc                                                                                                           <  2:520424/141‑1 (MQ=255)                                cTAAACAATTTTATAAAGATAAAATCAACGGAAAGCATAAAGTTGATGTTTTATTAGGAAGTTCATCTAAATATTTCGGTAAAAGTAATGGCAATTTAGATAAAAAATTCAAAAAAGATGGTTACGACCTTGCCACGAACa                                                                                                          <  1:686478/141‑1 (MQ=255)                                cTAAACAATTTTAAAAAGATAAAATCAACGGAAAGCATAAAGGTGATGTTTTATTAGGCGGCGGCGCGAAATATTTCGGTAAAAGTAATGGCAATTTAGATAAAAAATTCAAAAAAGATGGTTACGACCTTGCCACGAACa                                                                                                          <  1:686750/141‑1 (MQ=255)                                    aCAATTTTATAAAGATAAAATCAACGGAAAGCATAAAGTTGATGTTTTATTAGGCGGCGGCGCTAAATATTTCGGTAAAAGTAATGGCAATTTAGATAAAAAATTCAAAAAAGATGGTTACGACCTTGCCACGAACAGTaa                                                                                                      <  1:809493/141‑1 (MQ=255)                                     cAATTTTATAAAGATAAAATCAACGGAAAGCATAAAGTTGATGTTTTATTAGGCGGCGGTGCTAAATATTTCGGTAAAAGTAATGGCAATTTAGATAAAAAATTCAAAAAAGATGGTTACGACCTTGCCACGAACAGTAAg                                                                                                     >  2:260530/1‑141 (MQ=255)                                       aTTTTATAAAGATAAAATCAACGGAAAGCATAAAGTTGATGTTTTATTAGGCGGCGGCGCTAAATATTTCGGTAAAAGTAATGGCAATTTAGATAAAAAATTCAAAAAAGATGGTTACGACCTTGCCACGAACAGTAAGGa                                                                                                   <  2:510218/141‑1 (MQ=255)                                          ttATAAAGATAAAATCAACGGAAAGCATAAAGTTGATGTTTTATTAGGCGGCGGCGCTAAATATTTCGGTAAAAGTAATGGCAATTTAGATAAAAAATTCAAAAAAGATGGTTACGCCCTTGCCCCGAACAGTCAGGAGtt                                                                                                >  1:336097/1‑141 (MQ=255)                                             taaagcaaacaccAACGGAAAACTTAAAGTTGATGTTGTATTAGGTGGCGGCGCTAAATATTCCGGTAAAAGTAATGGCAATTTAGATAAAAAATTCAAAAAAGTTGGTTACGACCTTGCCACGAACAGTAAGGAGTTATc                                                                                             <  1:105195/129‑1 (MQ=255)                                             taAAGATAAAATCAACGGAAACCATAAAGTTGATGTTTTATTAGGCGCCGGCGCTAAATATTTCGGTAAAAGTAATTGCAATTTAGATAAAAAATTCAAAAAAGATGTTTACTACCTTGCCACGAACAGTAAGGAGTTATc                                                                                             <  1:318252/141‑1 (MQ=255)                                                  aTAAAATCAACGGAAAGCATAAAGTTGATGTTTTATTAGGCGGCGGCGCTAAATATTTCGGTAAAAGTAATGGCAATTTAGATAAAAAATACAAAAAAGATGGTTACGACCTTGCCACGAACAGTAAGGAGTTATCAAAAt                                                                                        <  2:64578/141‑1 (MQ=255)                                                   tAAAATCAACGGAAAGCATAAAGTTGATGTTTTATTAGGCGGCGGCGCTAAATATTTCGGTAAAAGTAATGGCAATTTAGATAAAAAATTCAAAAAAGATGGTTACGACCTTGCCACGAACAGTAAGGAGTTATCAAAATc                                                                                       >  2:796786/1‑141 (MQ=255)                                                   aaaaaTCAACGGAAATGATAAAGTTGATGTTTTATTAGGAGGCGGAGCTAAATATTTAGGTAAAAGTAATGGCAATTTAGAAAAAAAAATAAAAAAAGATGGTTACGACCTTGCCACGAACAGTAAGGAGTTATCAAAATc                                                                                       <  2:101278/140‑1 (MQ=255)                                                    aagagaaaCGGAAAGAAAAAAGTGGATGATTTATTAGGCGGCGGCGCAAAATATGTCAGTAAAAGTAATGGCAATTGAGAAAAAAAAAAAAAAAAAGATGGTTACGACCTTGCCACGAACAGTAAGGAGTTATCAAAATCt                                                                                      <  2:208833/135‑1 (MQ=255)                                                    aaaaACAAAGGAAAGCATAAAGTGGATGTCTGATTAGGCGGCGGCGATAAATATTTCGGTAAAAGTAATGGCAATTTAGAAAAAAAAAAAAAAAAAGATGGTTACGACCTTGCCACGAACAGTAAGGAGTTATCAAAATCt                                                                                      <  2:246988/141‑1 (MQ=255)                                                      aaTCAACGGAAAGCATAAAGTTGATGTTTTATTAGGCGGCGGCGCTAAATATTTCGGTAAAAGTAATGGCAATTTAGATAAAAAATTCAAAAAAGATGGTTACGACCTTGCCACGAACAGTAAGGAGTTATCAAAATCTga                                                                                    >  1:251860/1‑141 (MQ=255)                                                           aCGGAAAGCATAAAGTTGATGTTTTATTAGGCGGCGGCGCTAAATATTTCGGTAAAAGTAATGGCAATTTAGATAAAAAATTCAAAAAAGATGGTTACGACCTTGCCACGAACAGTAAGGAGTTATCAAAATCTgataaag                                                                               >  1:715919/1‑141 (MQ=255)                                                              gAAAGCATAAAGTTGATGTTTTATTAGGCGGCGGCGCTAAATATTTCGGTAAAAGTAATGGCAATTTAGATAAAAAATTCAAAAAAGATGGTTACGACCTTGCCACGAACAGTAAGGAGTTATCAAAATCTgataaagata                                                                            <  1:4770/141‑1 (MQ=255)                                                              gAAAGCATAAAGTTGATGTTTTATTAGGCGGCGGCGCTAAATATTTCGGTAAAAGTAATGGCAATTTAGATAAAAAATTAAAAAAAGGCGGTTAGGACCTTGCCACGAACAGTAAGGAGTTATCAAAATCTgataaagata                                                                            >  1:261768/1‑141 (MQ=255)                                                              gAAAGCATAAAGTTGATGTTTTATTAGGCGGCGGCGCTAAATATGTCGGTAAAAGGAATGGCAATTTAGATAAAAAATTCAAAAAAGATGGTTACGACCTTGCCACGAACAGTAAGGAGTTATCAAAATCTgataaagata                                                                            >  1:141691/1‑141 (MQ=255)                                                              gAAAGCATAAAGTTGATGTTTTATTAGGCGGCGGCGCTAAAGATTTCGGTAAAAGTAATGGCAATTTAGATAAAAAATTCAAAAAAGATGGTTACGACCTTGCCACGAACAGTAAGGAGTTATCAAAATCTgataaagata                                                                            <  1:22298/141‑1 (MQ=255)                                                               aaaGCATAAAGTTGATGTTTTATTAGGCGGCGGCGCTAAATATTTCGGTAAAAGTAATGGCAATTTAGATAAAAAATTCAAAAAAGATGGTTACGACCTTGCCACGAACAGTAAGGAGTTATCAAAATCTgataaagataa                                                                           <  1:297636/141‑1 (MQ=255)                                                               aaaGCATAAAGTTGATGTTTTATTAGGCGGCGGCGCTAAATATTTCGGTAAAAGTAATGGCAATTTAGATAAAAAATTCAAAAAAGATGGTTACGACCTTGCCACGAACAGTAAGGAGTTATCAAAATCTgataaagataa                                                                           <  2:461033/141‑1 (MQ=255)                                                                     tAAGGTTGATGTTTGTTTGGGCAGGGGCGCTGACTATTTAGGTAACGGTGATTGCATTTTAAATAAAAAATTCAAAGAAGATGGTTACTTCCTGTGCACGAACAGGAAGGAGTTATCAAAATCTGATAAAGATAAAGTTCt                                                                     <  2:786065/141‑1 (MQ=255)                                                                     tAAAGTTGATGTTTTATTAGGCGGCGGCGCTAAATATTTCGGTAAAAGTAATGGCAATTTAGATAAAAAATTCAAAAAAGATGGTTACGACCTTGCCTCGAACAGTAAGGAGTTATCAAAATCTGATAAAGATAAAGTTCt                                                                     <  1:648621/141‑1 (MQ=255)                                                                     aaaaGTTGATGTTTTATTAGGCGGCGGGGCTGAAAATTCCTGTAAAAGTAATAGCAAATTAGATAAAAAATTCAAAAAATATGTGTACGACCTTTCCACGAACAGTAAGGAGTTATCAAAATCTGATAAAGATAAAGTTCt                                                                     <  2:785983/140‑1 (MQ=255)                                                                        aGTTGATGTTTTATTAGGCGGCGGCGCTAAATATTTCGGTAAAAGTAATGGCAATTTAGATAAAAAATTCAAAAAAGATGGTTACGACCTTGCCACGAACAGTAAGGAGTTATCAAAATCCGATAAAGATAAAGTTCTTgg                                                                  <  1:662386/141‑1 (MQ=255)                                                                        aGTTGATGTTTTATTAGGCGGCCGCGCTAAAGAGTTCGGAAAACGATTTGGCGAATGAAATAAAAAATTCAAAAGAGATGGTTACGACCTTGCCACGAACAGTAAGGAGTTATCAAAATCTGATAAAGATAAAGTTCTTgg                                                                  <  1:213565/141‑1 (MQ=255)                                                                         gTTGATGTTTTATTAGGTGGCGGCGCTAAATATTTCGGTAAAAGTAATGGCAATTTAGATAAAAAATTCAAAAAAGATGGTTACGACCTTGCCACGAACAGTAAGGAGTTATCAAAATCTGATAAAGATAAAGTTCTTGgt                                                                 <  2:841218/141‑1 (MQ=255)                                                                          ttGATGTTTTATTAGGCGGCGACGCTAAATATTTCGGTAAAAGTAATGGCAATTTAGATAAAAAATTCAAAAAAGATGGTTACGACCTTGCCACGAACAGTAAGGAGTTATCAAAATCTGATAAAGATAAAGTTCTTGgtt                                                                >  1:485014/1‑141 (MQ=255)                                                                               gTTTTATTAGGCGGCGGCGCTAAATATTTCGGTAAAAGTAATGGCAATTTAGATAAAAAATTCAAAAAAGATGGTTACGACCTTGCCACGAACAGTAAGGAGTTATCAAAATCTGATAAAGATAAAGTTCTTGgtttgttt                                                           <  1:657812/141‑1 (MQ=255)                                                                               gTTTTATTAGGCGGCGGCGCTAAATATTTCGGTAAAAGTAATGGCAATTTAGATAAAAAATTCAAAAAAGATGGTTACGACCTTGCCACGAACAGTAAGGAGTTATCAAAATCTGATAAAGATAAAGTTCTTGgtttgttt                                                           <  1:158655/141‑1 (MQ=255)                                                                                  ttataaGGCGACGGCGATAAATATTTAGGAAAAAGTAATGGCAATTTAAAAAAAAAAAAAAAAAAAGATGGTTACGACCTTGCCACGAACAGTAAGGAGTTATCAAAATCTGATAAAGATAAAGTTCTTGGTTTGTTTGCa                                                        <  2:113549/141‑1 (MQ=255)                                                                                      taGGCGGCGGTGCTAAATATTTCGGTAAAAGTAATGGCAATTTAGATAAAAAATTCAAAAAAGATGGTTACGACCTTGCCACGAACAGTAAGGAGTTATCAAAATCTGATAAAGATAAAGTTCTTGGTTTGTTTGCAGATa                                                    <  1:260530/141‑1 (MQ=255)                                                                                        ggcggcggcGCTAAATATTTTGGTAAAAGTAATGGCAATTTAGATAAAAAATTCAAAAAAGATGGTTACGACCTTGCCACGAACAGTAAGGAGTTATCAAAATCTGATAAAGATAAAGTTCTTGGTTTGTTTGCAGATaaa                                                  <  2:683077/141‑1 (MQ=255)                                                                                        ggcggcggcGCTAAATATTTCGGTAAAAGTAATGGCAATTTAGATAAAAAATTCAAAAAAGATGGTTACGACCTTGCCACGATCAGTAAGGAGTTATCAAAATCTGATAAAGATAAAGTTCTTGGTTTGTTTGCAGATaaa                                                  <  1:567620/141‑1 (MQ=255)                                                                                        ggcggcggcGCTAAATATTTCGGTAAAAGTAATGGCAATTTAGATAAAAAATTCAAAAAAGATGGTTACGACCTTGCCACGAACAGTAAGGAGTTATCAAAATCTGATAAAGATAAAGTTCTTGGTTTGTTTGCAGATaaa                                                  <  1:536875/141‑1 (MQ=255)                                                                                         gcggcggcgCTAAATATTTCGGTAAAAGTAATGGCAATTTAGATAAAAAATTCAAAAAAGATGGTTACGACCTTGCCACGAACAGTAAGGAGTTATCAAAATGTGATCAGGATAAAGTTCTTGGTTTGTTTGCAGTTaata                                                 >  1:745336/1‑139 (MQ=255)                                                                                            gcggcgCTAAATATTTCGGTAAAAGTAATGGCAATTTAGATAAAAAATTCAAAAAAGATGGTTACGACCTTGCCACGAACAGTAAGGAGTTATCAAAATCTGATAAAGATAAAGTTCTTGGTTTGTTTGCAGATAAAAata                                              <  1:78964/141‑1 (MQ=255)                                                                                             cggcgCTAAATATTTCGGTAAAAGTAATGGCAATTTAGATAAAAAATTCAAAAAAGATGGTTACGACCTTGCCACGAACAGTAAGGAGTTATCAAAATCTGATAAAGATAAAGTTCTTGGTTTGTTTGCAGATAAAAatat                                             <  2:898100/141‑1 (MQ=255)                                                                                               gcgcTAAATATTTCGGTAAAAGTAATGGCAATTTAGATAAAAAATTCAAAAAAGCTGGTTACGACCTTGCCACGAACAGTAAGGAGTTATCAAAATCTGATAAAGATAAAGTTCTTGGTTTGTTTGCAGCTAAAAATATGc                                           <  1:947019/141‑1 (MQ=255)                                                                                               gcgcTAAATATTTCGGTAAAAGTAATGGCAATTTAGATAAAAAATTCAAAAAAGATGGTTACGACCTTGCCACGAACAGTAAGGAGTTATCAAAATCTGATAAAGATAAAGTTCTTGGTGTGTTCGCAGATAAAAATATGc                                           <  1:484164/141‑1 (MQ=255)                                                                                               gcgcTAAATATTTCGGTAAAAGTAATGGCAATTTAGATAAAAAATTCAAAAAAGATGGTCAAGAGCTTTTCACGAACAGTAAGGAGTTATCAAAATCTGATAAAGATAAAGTTCTTGGTTTGTTTGCAGATAAAAATATGc                                           <  1:449089/141‑1 (MQ=255)                                                                                                cgcTAAATAGTTCGGTAAAAGTAATGGCAATTTAGATAAAAAATTCAAAAAAGATGGTTACGACCTTGCCACGAACAGTAAGGAGTTATCAAAATCTGATAAAGATAAAGTTCTTGGTTTGTTTGCAGATAAAAATATGcc                                          <  2:715919/141‑1 (MQ=255)                                                                                                 gcTAAATATTTCGGTAAAAGTAATGGCAATTTAGATAAAAAATTAGAAAAAGTCGGTTACGACCTTGCCACGAACAGTAAGGAGTTATCAAAATCTGATAAAGATAAAGTTCTTGGTTTGTTTGCAGATAAAAATATGCCa                                         >  1:366242/1‑141 (MQ=255)                                                                                                   tAAATATTTCGGTAAAAGTAATGGCAATTTAGATAAAAAATTCAAAAAAGATGGTTACGACCTTGCCACGAACAGTAAGGAGTTATCAAAATCTGATAAAGATAAAGTTCTTGGTTTGTTTGCAGATAAAAATATGCCACt                                       <  1:843091/141‑1 (MQ=255)                                                                                                   aaaaTATTTCGGTAAAAGTAATGGCAATTTAGATAAAAAAGTCAAAAAAGATGGTTACGACCTTGCCACGAACAGTAAGGAGTTATCAAAATCTGATAAAGATAAAGTTCTTGGTTTGTTTGCAGATAAAAATATGCCACt                                       <  2:772480/140‑1 (MQ=255)                                                                                                      atattaaacgaaaaGTAATTTCAAGTTAAATAAAAAAATAAAAACAGATAGTGACGACCTTGCCACGAACAGTAAGGATTTAACAAAATCTTATAAAGATAAAGTTCTTGGTTTGTTTGCAGATAAAAATATGCCACTTGc                                    <  2:420209/131‑1 (MQ=255)                                                                                                         tttCGGTAAAAGTAATGGCAATTTAGATAAAAAATTCAAAAAAGATGGTTACGACCTTGCCACGAACAGTAAGGAGTTATCAAAATCTGATAAAGATAAAGTTCTTGGTTTGTTTGCAGATAAAAATATGCCACTTGCAAt                                 >  2:436391/1‑141 (MQ=255)                                                                                                           tCGTTAAAAGTAATGGCAATTTAGATAAAAAATTCAAAAAATATGGTTACTACCTTGCCACGAACAGTAAGGAGTTATCAAAATCTGATAAAGATAAAGTTCTTGGTTTGTTTGCAGATAAAAATATGCCACTTGCAATAg                               <  2:359642/141‑1 (MQ=255)                                                                                                           tCGGTAAAAGTAATGGCAATTTAGATAAAAAATTCAAAAAAGATGGTTACGACCTTGCCACGAACAGTAAGGAGTTATCAAAATCTGATAAAGATAAAGTTCTTGGTTTGTTTGCAGATAAAAATATGCCACTTGCAATAg                               >  1:168614/1‑141 (MQ=255)                                                                                                             ggTAAAAGTAATGGCAATTTAGATAAAAAATTCAAAAAAGATGGTTACGACCTTGCCACGAACAGTAAGGAGTTATCAAAATCTGATAAAGATAAAGTTCTTGGTTTGTTTGCAGATAAAAATATGCCACTTGCAATAGAt                             <  1:654521/141‑1 (MQ=255)                                                                                                             ggTAAAAGTAATGGCAATTTAGATAAAAAATTCAAAAAAGATGGTTACGACCTTGCAACGAACAGTAAGGAGTTATCAAAATCTGATAAAGATAAAGTTCTTGGTTTGTTTGCAGATAAAAATATGACGCTTGCAATAGAt                             >  2:810748/1‑141 (MQ=255)                                                                                                             cgTAAAAGAAATGGCAATTTAGATAAAAAATTCAAAAAAGATTGTTACGACCTTGCCACGAACAGTAAGGAGTTATCAAAATCTGATAAAGATAAAGTTCTTGGTTTGTTTGCAGATAAAAATATGCCACTTGCAATAGAt                             <  1:350522/140‑1 (MQ=255)                                                                                                               tAAATGTAATACCAATATAGATAAAATATGCAAACAAGATTGTTTCCACCTTGACCCCAACAGTAAGGAGTTATCAGAATATGATGAAGATCAATTTCTTGGTTTGTTTGCAGATAAACATATGTCACTTGCAATAGATGc                           <  2:287237/141‑1 (MQ=255)                                                                                                               tAAAAGTAATGGCAATTTAGATAAAAAATTCAAAAAAGATGGTTACGACCTTGCCACGAACAGTAAGGAGTTATCAAAATCTGATAAAGATAAAGTTCTTGGTTTGTTTGCAGATAAAAATATGCCACTTGCAATAGATGc                           <  1:167783/141‑1 (MQ=255)                                                                                                                  aaGTAATGGCAATTTAGATAAAAAATTCAAAAAAGATGGTTACGACCTTGCCACGAACAGTAAGGAGTTATCAAAATCTGATAAAGATAAAGTTCTTGGTTTGTTTGCAGATAAAAATATGCCACTTGCAATAGATGCttt                        >  2:845331/1‑140 (MQ=255)                                                                                                                   aGTAATGGCAATTTAGATAAAAAATTCAAAAAAGATGGTTACGACCTTGCCACGAACAGTAAGGAGTTATCAAAATCTGATAAAGATAAAGTTCTTGGTTTGTTTGCAGATAAAAATATGCCACTTGCAATAGATGCTTCt                       <  1:321641/141‑1 (MQ=255)                                                                                                                        tGGCAATTTAGATAAAAAATTCAAAAAAGATGGTTACGCCCTTGCCACGAACAGTAAGGAGTTATCAAAATCTGATAAAGATAAAGTTCTTGGTTTGTTTGCAGATAAAAATATGCCACTTGCAATAGATGCTTCTAAAGa                  <  1:727458/141‑1 (MQ=255)                                                                                                                        tGGCAATTTAGATAAAAAACTCAAAAAAGATGGTTACGACCTTGCCACGAACAGTAAGGAGTTATCAAAATCTGATAAAGATAAAGTTCTTGGTTTGTTTGCAGATAAAAATATGCCACTTGCAATAGATGCTTCTAAAGa                  <  1:910441/141‑1 (MQ=255)                                                                                                                         ggCAATTTAGATAAAAAATTCAAAAAAGATGGTTACGACCTTGCCACGAACAGTAAGGAGTTATCAAAATCTGATAAAGATAAAGTTCTTGGTTTGTTTGCAGATAAAAATATGCCACTTGCAATAGATGCTTCTAAAGAc                 <  2:7686/141‑1 (MQ=255)                                                                                                                                  gATAAAAAATTCAAAAAAGATGGTTACGACCTTGCCACGAACAGTAAGGAGTTATCAAAATCTGATAAAGATAAAGTTCTTGGTTTGTTTGCAGATAAAAATATGACACTTGCAATAGATGCTTCTAAAGAAAAACcttca        >  2:783711/1‑141 (MQ=255)                                                                                                                                    aaaaaaaTTCAAAAAAGATGGTTACGACCTTGCCAAGAACAGTAAGGAGTTAGCAAAATCTGATTAAGATAAATTTCTTTGTTTGTTTGCAGATAAAAATATGCCACTTGCAATAGATGCTTCTAAAGACGAACCATCAtt      <  2:336097/140‑1 (MQ=255)                                                                                                                                     aaaaaaTTAAAAAAATATAGTTACGACCTTGCCACGAACAGTAAGGAGTTATCAAAATCTGATAAAGATAAAGTTCTTGGTTTGTTTGCAGATAAAAATATGCCACTTGCAATAGATGCTTCTAAAGACGAACCATCATTa     <  2:103737/141‑1 (MQ=255)                                                                                                                                        aaaTTCAAAAAAGATGGTTACGACCTTGCCACGAACAGTAAGGAGTTATCAAAATCTGATAAAGATAAAGTTCTTGGTTTGTTTGCAGATAAAAATATGCCACTTGCAATAGATGCTTCTAAAGACGAACCATCATTAGCt  <  1:645449/141‑1 (MQ=255)                                                                                                                                        aaaTTCAAAAAAGATGGTTACGACCTTGCCACGAACAGTAAGGAGTTATCAAAATCTGATAAAGATAAAGTTCTTGGTTTGTTTGCAGATAAAAATATGCCACTTGCAATAGATGCTTCTAAAGACGAACCATCATTAGCt  <  1:796786/141‑1 (MQ=255)                                                                                                                                             |                                                                                                                                        GTGACTTCTAGAGATGATAAAAACGAAATCGCTAAACAATTTTATAAAGATAAAATCAACGGAAAGCATAAAGTTGATGTTTTATTAGGCGGCGGCGCTAAATATTTCGGTAAAAGTAATGGCAATTTAGATAAAAAATTCAAAAAAGATGGTTACGACCTTGCCACGAACAGTAAGGAGTTATCAAAATCTGATAAAGATAAAGTTCTTGGTTTGTTTGCAGATAAAAATATGCCACTTGCAATAGATGCTTCTAAAGACGAACCATCATTAGCT  >  CP000730/2771703‑2771978

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑