Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	26,506	C→G	R281G (CGC→GGC)	vicK →	sensor histidine kinase VicK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	26,506	0	C	G	100.0%	131.3 / NA	38	R281G (CGC→GGC)	vicK	sensor histidine kinase VicK
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (20/18);  total (20/18)

GGCGAATTAGCTTTAGCATTTAATAACTTGTCTAAACGTGTACAAGAAGCGCAGGCTAATACTGAAAGTGAGAAACGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACCGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAAAGAAGACATCATCGGATATTACATGTTAAGTGTATTAAGTCTTGAAGATGAATTTAAACTGGAAGAAATTCAAGAGA  >  CP000730/26377‑26641                                                                                                                                  |                                                                                                                                        ggCGAATTAGCTTTAGCATTTAATAACTTGTCTAAACGTGTACAAGAAGCGCAGGCTAATACTGAAAGTGAGAAACGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGt                                                                                                                              <  1:57930/141‑1 (MQ=255)         aGCTTTAGCATTTAATAACTTGTCTAAACGTGTACAAGAAGCGCAGGCTAATACTGAAAGTGAGAAACGTAGACTGGCCTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTAt                                                                                                                      >  2:168264/1‑141 (MQ=255)                           cTTGTCTAAACGTGTACAAGAAGCGCAGGCTAATACTGAAAGTGAGAAACGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACt                                                                                                    >  1:31310/1‑141 (MQ=255)                            ttGTCTAAACGTGTACAAGAAGCGCAGGCTAATACTGAAAGTGAGAAACGTGGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTc                                                                                                   <  1:150422/141‑1 (MQ=255)                            ttGTCTAAACGTGTACAAGAAGCGCAGGCTAATACTGAAAGTGAGAAACGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGatat                                                                                                          >  1:277695/1‑134 (MQ=255)                            ttGTCTAAACGTGTACAAGAAGCGCAGGCTAATACTGAAAGTGAGAAACGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGatat                                                                                                          <  2:277695/134‑1 (MQ=255)                               tCTAAACGTGTACAAGAAGCGCAGGCTAATACTGAAAGTGAGAAACGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAg                                                                                                <  2:269552/141‑1 (MQ=255)                                    aCGTGTACAAGAAGCGCAGGCTAATACTGAAAGTGAGAAACGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCt                                                                                           <  2:133508/141‑1 (MQ=255)                                     cGTGTACAAGAAGCGCAGGCTAATACTGAAAGTGAGAAACGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCtt                                                                                          <  2:74710/141‑1 (MQ=255)                                             agaagCGCAGGCTAATACTGAAAGTGAGAAACGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGc                                                                                  >  1:69304/1‑141 (MQ=255)                                              gaagCGCAGGCTAATACTGAAAGTGAGAAACGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCg                                                                                 <  2:216605/141‑1 (MQ=255)                                               aagCGCAGGCTAATACTGAAAGTGAGAAACGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAACCGGGCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGa                                                                                >  1:201294/1‑141 (MQ=255)                                               aagCGAAGGCTAATACTGAAAGTGAGAAACGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGa                                                                                <  2:14029/141‑1 (MQ=255)                                                agCGCAGGCTAATACTGAAAGGGAGAAACGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTTCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGaa                                                                               <  2:17299/141‑1 (MQ=255)                                                    cAGGCTAATACTGAAAGTGAGAAACGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAaagaa                                                                           >  1:20247/1‑141 (MQ=255)                                                     aGGCTAATACTGAAAGTGAGAAACGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAaagaag                                                                          >  2:25766/1‑141 (MQ=255)                                                       gCTAATACTGAAAGTGAGAAACGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAAAGAAGAc                                                                        <  2:20247/141‑1 (MQ=255)                                                              cTGAAAGTGAGAAACGTAGACTGTACTCAGTTATCGCCTATGTGGGTTATTTGATTTTTTTAACATTCGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAAAGAAGACATCATCg                                                                 <  1:236033/141‑1 (MQ=255)                                                              cTGAAAGTGAGAAACGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCCCTCAAGATGCTTGGTATGGCGAAAGAAGACATCATCg                                                                 <  2:150103/141‑1 (MQ=255)                                                                gAAAGTGAGAAACGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGACAGAAGAcatcagctga                                                               >  2:150276/1‑136 (MQ=255)                                                                   aGTGAGAAACGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAAAGAAGACATCATCGGatat                                                            >  1:165446/1‑141 (MQ=255)                                                                          aaCGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATg                                                                                                         <  1:47452/89‑1 (MQ=255)                                                                          aaCGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATg                                                                                                         >  2:47452/1‑89 (MQ=255)                                                                          aaCGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAAAGAAGACATCATCGGATATTACATGt                                                     >  2:121931/1‑141 (MQ=255)                                                                                        tCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAAAGAAGACATCATCGGATATTACATGTTAAGTGTATTAAGt                                       <  1:219498/141‑1 (MQ=255)                                                                                         cAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGACAGACGAACTCCTCGGATATTACATGTTAAGTGTATTAAGTc                                      >  1:266519/1‑141 (MQ=255)                                                                                          aGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAAAGAAGACATCATCGGATATTACATGTTAAGTGTATTAAGTCt                                     <  1:121931/141‑1 (MQ=255)                                                                                              aTCACTCATATGGGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAAAGAAGACATCATCGGATATTACATGTTAAGTGTATTAAGTCTTGaa                                 >  1:94313/1‑141 (MQ=255)                                                                                                 aCCCATATGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAAAGAAGACATCATCGGATATTACATGTTAAGTGTATTAAGTCTTGAAGAt                              >  1:88824/1‑141 (MQ=255)                                                                                                      tatGAGTGATGGTATTATTTTAAGATACGGGATTAGTCGTATTCGTATCTTTAATGATATGGCACTAAAGATGCTTTATATGGCGAAATAAGACATCATCAGATAATACATATTAAGTGTATTTGGTCTTGAACATGAAtt                         <  2:100812/141‑1 (MQ=255)                                                                                                        tGAGTGATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAAAGAAGACATCATCGGATATTACATGTTAAGTGTATTAAGTCTTGAAGATGAATTTa                       >  2:216406/1‑141 (MQ=255)                                                                                                          aGTGATGGTATTATTGCAATAGACGGCCGTGGACGTATTCGTATCGTCAAGGATATGGCACTCAAGATGCTTGGTATGGCGAAAGAAGACATCATCGGATATTACATGTTAAGTGTATTAAGTCTTGAAGGTGAATTTaaa                     >  2:56820/1‑141 (MQ=255)                                                                                                             gATGGTATTATTGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAAAGAAGACATCATCGGATATTACATGTTAAGTGTATTAAGTATTGAAGATGAATTTAAACTg                  >  2:118961/1‑141 (MQ=255)                                                                                                                   attattGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAAAGAAGACATCATCGGATATTACATGTTAAGTGTATTAAGTCTTGAAGATGAATTTAAACTGgaaga             <  1:33513/140‑1 (MQ=255)                                                                                                                   attattGCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAAAGAAGACATCATCGGATATTACATGTTAAGTGTATTAAGTCTTGAAGATGAATTTAAACTGgaaga             >  2:33513/1‑140 (MQ=255)                                                                                                                         gCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAAAGAAGACATCATCGGATATTACATGTTAAGTGTATTAAGTCTTGAAGATGAATTTAAACTGGAAGAAATTCaa      >  1:199893/1‑141 (MQ=255)                                                                                                                         gCAACAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAAAGAAGACATCATCGGATATTACATGTTAAGTGTATTAAGTCTTGAAGATGAATTTAAACTGGAAGAAATTCaa      <  2:225960/141‑1 (MQ=255)                                                                                                                             cAGACGGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAAAGAAGACATCATCGGATATTAAATGTTAAGTGTATTAAGTCTTGAAGATGAATTTAAACTGGAAGAAATTCAAgaga  >  2:114657/1‑141 (MQ=255)                                                                                                                                  |                                                                                                                                        GGCGAATTAGCTTTAGCATTTAATAACTTGTCTAAACGTGTACAAGAAGCGCAGGCTAATACTGAAAGTGAGAAACGTAGACTGGACTCAGTTATCACCCATATGAGTGATGGTATTATTGCAACAGACCGCCGTGGACGTATTCGTATCGTCAATGATATGGCACTCAAGATGCTTGGTATGGCGAAAGAAGACATCATCGGATATTACATGTTAAGTGTATTAAGTCTTGAAGATGAATTTAAACTGGAAGAAATTCAAGAGA  >  CP000730/26377‑26641

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑