Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	603,961	G→A	M465I (ATG→ATA)	araB →	ribulokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	603,961	0	G	A	100.0%	83.0 / NA	25	M465I (ATG→ATA)	araB	ribulokinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/10);  total (15/10)

TAAGATGATTATGCAACAGTATCAAGATTGGCATATGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATGGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGATGCTATGAAAGAGCCAATTTTATACGAAATTAATCCAGAAGCGGA  >  CP000730/603826‑604092                                                                                                                                        |                                                                                                                                    tAAGATGATTATGCAACAGTATCAAGATTGGCATATGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTatagatag                                                                                                                                <  1:15842/141‑1 (MQ=255)      tgatTATGCAACAGTATCAAGATTGGCATATGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGt                                                                                                                           >  2:152051/1‑141 (MQ=255)           aTGCAACAGTATCAAGATTGGCATATGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCa                                                                                                                      >  2:184303/1‑141 (MQ=255)                     aTCAAGATTGGCATATGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAg                                                                                                            >  1:17910/1‑141 (MQ=255)                      tCAAGATTGGCATATGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAgg                                                                                                           >  1:270550/1‑141 (MQ=255)                          gATTGGCATATGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCgca                                                                                                       <  1:184303/141‑1 (MQ=255)                          gATTGGCATATGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCgca                                                                                                       <  1:272075/141‑1 (MQ=255)                            ttGGCATATGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCgcagc                                                                                                     <  2:78091/141‑1 (MQ=255)                                 atatGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAatat                                                                                                >  1:142758/1‑141 (MQ=255)                                           aGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGcagt                                                                                      >  2:125083/1‑141 (MQ=255)                                                      ttGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGgcg                                                                           <  2:17910/141‑1 (MQ=255)                                                       tGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGgcgc                                                                          >  1:36926/1‑141 (MQ=255)                                                                     taCCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTa                                                            >  1:10389/1‑141 (MQ=255)                                                                                  tGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGa                                               <  2:270550/141‑1 (MQ=255)                                                                                   gCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGAt                                              >  1:58909/1‑141 (MQ=255)                                                                                    cTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGATg                                             <  1:240247/141‑1 (MQ=255)                                                                                               gATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGATGCTATGAAagag                                  <  1:152051/141‑1 (MQ=255)                                                                                                atatCGATGTGAATGTACTGTATAACAAACTAATTGTTATAGATAGTGAGAATGCACAAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGCTGCTATGaaggtgc                                 <  2:185229/141‑6 (MQ=255)                                                                                                 tatCTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGATGCTATGAAAGAGcc                                <  2:142758/141‑1 (MQ=255)                                                                                                    cTATGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGATGCTATGAAAGAGCCAAt                             >  2:3780/1‑141 (MQ=255)                                                                                                      aTGCGAATGTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGATGCTATGAAAGAGCCAAttt                           >  1:141349/1‑141 (MQ=255)                                                                                                              gTACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGGGGTGGCGCATATAATTCAATTAATCACGCAGTTGATGCTATGAAAGAGCCAATTTTAAACGaa                   >  2:6421/1‑141 (MQ=255)                                                                                                              gTACTGAATAAAAAACTAATTGTAATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGATGCTATGAAAGAGCCAATTTTATACGaa                   >  2:6428/1‑141 (MQ=255)                                                                                                               tACTGAATAAAAAACTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGATGCTATGAAAGAGCCAATTTTATACGaaa                  <  2:277017/141‑1 (MQ=255)                                                                                                                           aaCTAATTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTAAATTAATGACGCAGTTGATGCTATGAAAGAGCCAATTTTATACGAAATTAATCCAGAAg      >  1:60578/1‑141 (MQ=255)                                                                                                                               aaTTGTTATAGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGACGCACATAATTCAATTAATGACGCAGTTGATGCTATGAAAGAGCCAATTTTATACGAAATTAATCCAGAAGCGGa  >  2:18732/1‑141 (MQ=255)                                                                                                                                        |                                                                                                                                    TAAGATGATTATGCAACAGTATCAAGATTGGCATATGGAAGTAGAAAAGGTATTTGCATGTGGCGGTATACCTAAAAAGAATGCTGTTATGATGGATATCTATGCGAATGTACTGAATAAAAAACTAATTGTTATGGATAGTGAGTATGCACCAGCAATAGGCGCAGCAATATTAGGTGCAGTCAGTGGTGGCGCACATAATTCAATTAATGACGCAGTTGATGCTATGAAAGAGCCAATTTTATACGAAATTAATCCAGAAGCGGA  >  CP000730/603826‑604092

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑