Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	1,899,742	A→G	N421N (AAT→AAC)	USA300HOU_1761 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,899,742	0	A	G	100.0%	75.9 / NA	23	N421N (AAT→AAC)	USA300HOU_1761	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/14);  total (9/14)

TAGTTTATTAACACCGTTTGTCATTTGACCTATCGCAATCATATTTGTTAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTATTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTTTTGCACCTGTCGCGTTAATGACAACATTATATATGTCTACTGATTCTTGGTTTTTGTATGAAAAATAATACAACTTGCCATC  >  CP000730/1899607‑1899879                                                                                                                                        |                                                                                                                                          tAGTTTATTAACACCGTTTGTCATTTGACCTATCGCAATCATATTTGTTAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTc                                                                                                                                      <  1:234548/141‑1 (MQ=255)   gTTTATTAACACCGTTTGTCATTTGACCTATCGCAATCATATTTGTTAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCaa                                                                                                                                    <  1:158115/141‑1 (MQ=255)       attaACACCGTTTGTCATTTGACCTATCGCAATCATATTTGTTAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGAtt                                                                                                                                >  2:72953/1‑141 (MQ=255)                     tCATTTGACCTATCGCAATCATATTTGTTAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGat                                                                                                                  >  1:129833/1‑141 (MQ=255)                                  cGCAATCATATTTGTTAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCaa                                                                                                     <  1:111735/141‑1 (MQ=255)                                           atTTGTTAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTaa                                                                                            >  1:162139/1‑141 (MQ=255)                                             ttGTTAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAAt                                                                                          <  1:130952/141‑1 (MQ=255)                                                ttAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGAc                                                                                       >  2:238787/1‑141 (MQ=255)                                                ttAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGAc                                                                                       <  1:238787/141‑1 (MQ=255)                                                  aaTGTTCCAATCCTTGGTCTGATAACTTGATTGATTTACGGAATGATTTGTATGCTTTCCATTGGGTGTGCTTGTACAATCTGTCTGTTTTCAATATTTCTAATTAATTTAAGATCTTGCTCCATTTCATTTAAATGACtt                                                                                     <  2:277044/141‑1 (MQ=255)                                                            aCCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTTTTGCACCTg                                                                           <  2:26657/141‑1 (MQ=255)                                                               ttGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTTTTGCACCTGTcg                                                                        <  2:242704/141‑1 (MQ=255)                                                                 ggACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTTTTGCACCTGTcgcg                                                                      <  2:161949/141‑1 (MQ=255)                                                                         aaCTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTTTTGCACCGGTCGCGTTAATGac                                                              <  1:135628/141‑1 (MQ=255)                                                                          aCTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTTTTGCACCTGTCGCGTTAATGaca                                                             <  2:209631/141‑1 (MQ=255)                                                                                  ggTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTTTTGCACCTGTCGCGTTAATGACAACATtata                                                     <  2:158147/141‑1 (MQ=255)                                                                                             tGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTTTTGCACCTGTCGCGTTAATGACAACATTATATATGTCTACTg                                          >  1:37684/1‑141 (MQ=255)                                                                                                 ttGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTTTTGCACCTGTCGCGTTAATGACAACATTATATATGTCTACTGATTc                                      <  2:124612/141‑1 (MQ=255)                                                                                                        ccTCCCATTGGGTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTTTTGCACCTGTCGCGTTAATGACAACATTATATATGTCTACTGATTCTTGGttt                               >  1:126475/1‑141 (MQ=255)                                                                                                                  ggTGTGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTTTTGCACCTGTCGCGTTAATGACAACATTATATATGTCTACTGATTCTTGGTTTTTGTATGaaa                     >  1:112986/1‑141 (MQ=255)                                                                                                                   gtgtGCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTTTTGCACCTGTCGCGTTAATGACAACATTATATATGTCTACTGATTCTTGGTTTTTGTATGaaaa                    >  1:154611/1‑141 (MQ=255)                                                                                                                       gCTTGTACAATTTGTCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTTTTGCACCTGTCGCGTTAATGACAACATTATATATGTCTACTGATTCTTGGTTTTTGTATGAAAaataa                >  2:67189/1‑141 (MQ=255)                                                                                                                                     tCTGTTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTTTTGCACCTGTCGCGTTAATGACAACATTATATATGTCTACTGATTCTTGGAAAAAAAAAAAAAAATAATACAACTTGCcatc  <  2:37684/141‑1 (MQ=255)                                                                                                                                        |                                                                                                                                          TAGTTTATTAACACCGTTTGTCATTTGACCTATCGCAATCATATTTGTTAATGTTCCAAACCTTGGACTAATAACTTGATTGGTTTCCGGAATGATTTGTATGCCTCCCATTGGGTGTGCTTGTACAATTTGTCTATTTTCAAGATTTCTAATTAATTGATCATCTTGATCCAATTCATTTAAATGACTTTTTGCACCTGTCGCGTTAATGACAACATTATATATGTCTACTGATTCTTGGTTTTTGTATGAAAAATAATACAACTTGCCATC  >  CP000730/1899607‑1899879

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑