Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,455,692 Δ1 bp 59.4% coding (1210/1260 nt) USA300HOU_2315 → hypothetical membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,455,6920G.59.4% 119.5 / 212.7 123coding (1210/1260 nt)USA300HOU_2315hypothetical membrane protein
Reads supporting (aligned to +/- strand):  ref base G (23/13);  new base . (36/37);  total (67/56)
Fisher's exact test for biased strand distribution p-value = 1.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.48e-01

Only 100 of 131 total aligned reads displayed.

GAATCAGCAGAATCAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAATTTTAACACCC  >  CP000730/2455554‑2455832
                                                                                                                                          |                                                                                                                                            
gAATCAGCAGAATCAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCaaaa                                                                                                                                            >  1:384229/1‑141 (MQ=255)
      gcagAATCAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAaaaaaatta                                                                                                                                      <  1:1199384/141‑4 (MQ=255)
         gAATCAGTTAAACAAGCACCACAGAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAaaaaaattatcg                                                                                                                                   >  2:1017634/1‑135 (MQ=255)
         gAATCAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAaaaaaattatcg                                                                                                                                   <  2:1006411/141‑7 (MQ=255)
         gAATCAGTTAAACAAGCACCACAAAGTGACGATTTAAAAAACTATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATc                                                                                                                                   <  2:65555/141‑1 (MQ=255)
          aaTCAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCg                                                                                                                                  >  2:1075839/1‑141 (MQ=255)
            tCAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGaaa                                                                                                                               <  1:668845/141‑1 (MQ=255)
              aGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGaaaaa                                                                                                                             <  2:1051871/141‑1 (MQ=255)
               aatAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACCTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGaaaaat                                                                                                                            <  2:320077/139‑2 (MQ=255)
                  aaaCAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTg                                                                                                                         <  2:976007/141‑1 (MQ=255)
                    aCAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGAAAAAACTga                                                                                                                        <  2:30077/141‑1 (MQ=255)
                       aGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTgaagaa                                                                                                                    <  1:5037/141‑1 (MQ=255)
                       aGCACCAAAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAAAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTgaagaa                                                                                                                    <  2:270473/141‑1 (MQ=255)
                        gCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGGACCTGCGACTTATAAAGAAGACAGACGTTCAGCAGTTTTATTTGATGCAAAAAAATTATCGAAAAAAcgtataaac                                                                                                                   >  1:1146756/1‑133 (MQ=255)
                             acaAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCt                                                                                                              >  2:950414/1‑141 (MQ=255)
                                aaGTGACGATTTAACAAACGATTCAACTGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGATGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCa                                                                                                           <  1:723515/141‑1 (MQ=255)
                                  gTGACGATGTAACGAACGATTCAAAGGAAGATGAAATAGAGGAATCATTAAAAGAACCTGCGAGTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGa                                                                                                         <  2:809519/141‑1 (MQ=255)
                                   tGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGAt                                                                                                        <  1:1202121/141‑1 (MQ=255)
                                   gcaacaTTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGTGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGAt                                                                                                        <  2:224040/136‑1 (MQ=255)
                                    gACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGAAAAAACTGAAGAACAATCTTCAgca                                                                                                        >  2:626699/1‑139 (MQ=255)
                                     aagATATAACAAACGATTCAAATAAAGATGAAAAAAAGCAATCATTAAAAGAACCTGTGACTTATAAAGAAGACAGACTTTCATCAGTTGTAATTGATGCAAAAAAAAAAAAAAAAAAACTGAAGAACAATCTTCAGATaa                                                                                                      <  2:242265/139‑1 (MQ=255)
                                     aCGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTGCAGATaa                                                                                                      >  2:954802/1‑141 (MQ=255)
                                       gATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGAAAAAACTGAAGAACAATCTTCAGATaaa                                                                                                     <  1:100035/141‑1 (MQ=255)
                                       gATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATaaaa                                                                                                    >  1:701066/1‑141 (MQ=255)
                                       gATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACCTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGAAAAAACTGAAGAACAATCTTCAGATaaa                                                                                                     >  2:298780/1‑141 (MQ=255)
                                             aCAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAaat                                                                                              >  2:351522/1‑141 (MQ=255)
                                                aaCGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAaataa                                                                                            >  1:47206/1‑141 (MQ=255)
                                                  cGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAAtaataa                                                                                         >  2:1170743/1‑141 (MQ=255)
                                                  cGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAataatac                                                                                         >  1:297881/1‑140 (MQ=255)
                                                  agATTGAAATGAAGATAAAATAAAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAAAAAAAAAAAAAACTGAAGAACAATCTTCAGATAAAAACAAAtaataa                                                                                         <  2:925442/140‑1 (MQ=255)
                                                     atCAAATAAAGACAAAATAGAACAATAATTAAAAGAACCAGCGACTTATAAAGAAGACAGACGTACATCAGTTGAAATTGATACAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACaa                                                                                      <  2:1097263/140‑1 (MQ=255)
                                                      tCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACaa                                                                                      <  2:807197/141‑1 (MQ=255)
                                                        aaaTGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAgc                                                                                   >  1:187264/1‑141 (MQ=255)
                                                         aaTGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAgcg                                                                                  >  2:811129/1‑141 (MQ=255)
                                                         aaTGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAgcg                                                                                  >  2:919394/1‑141 (MQ=255)
                                                         aaTGAAGATGAAATAGAGCAACCATTAAAAGAACCTGCTCCTCATAAAGCAGACCGACAGACATCAGCACATTCGGATGTAAAAAAATCATCGAAAAAACTAAAGAACAATCTTCAGATAAAACCAAAAAAAAACaacacg                                                                                  >  2:400625/1‑137 (MQ=255)
                                                             aaGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCtt                                                                               >  1:1029348/1‑141 (MQ=255)
                                                               gATTAAATAGAGCAATCATTAAAAGAACCTGCGACGTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAACAAACTGCAGAACAATCTTCAGATCAAACCAACTAATAACAAAGCGCTTGCt                                                                            <  2:699278/141‑1 (MQ=255)
                                                               gATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCt                                                                            <  1:941719/141‑1 (MQ=255)
                                                                 tGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCATGCTac                                                                          >  2:969635/1‑140 (MQ=255)
                                                                 agAAATAGAGCAATCATAAAAAGAACCTGCGACTTATAAAGGAGACAGACGTTCATCAGTTGAAAAAAAAGAAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAg                                                                          <  1:404689/140‑1 (MQ=255)
                                                                  gAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAg                                                                          >  1:603892/1‑141 (MQ=255)
                                                                   aaaTAGAGCAATCATTAAAAGAACCTGAGACTTATAAAGAAGACAGACGTTCATGGGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATGTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTa                                                                        >  1:996885/1‑141 (MQ=255)
                                                                      aagagGAAACATTAAAAGAACCTGCGACTTACAAAGAAGAAAGAAGTTCATCAGTTGTAATTGATGCAGAAAAAAATAAAAAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTAcc                                                                      <  2:27484/140‑1 (MQ=255)
                                                                       agagCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACctc                                                                    >  2:649973/1‑141 (MQ=255)
                                                                       agaaCAAGCATTAAAAAAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGACAAAACTGAAGAACAAGTTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACctc                                                                    <  2:492301/141‑1 (MQ=255)
                                                                        gagCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACctct                                                                   <  1:898236/141‑1 (MQ=255)
                                                                        gagCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACctct                                                                   <  2:178035/141‑1 (MQ=255)
                                                                          gCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGCTGTAATTGATGCAGAAAAACATATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCtt                                                                  >  2:879240/1‑141 (MQ=255)
                                                                           cAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTaa                                                                >  1:686918/1‑141 (MQ=255)
                                                                            aaTCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTAGCTAGTACCTCTTaa                                                                >  1:328332/1‑141 (MQ=255)
                                                                             agagatAAAAAAACCTGCGACTTAGAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAACGCGCTTGCTAGTACCTCTTaaaa                                                              <  2:434281/136‑1 (MQ=255)
                                                                             aTCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTaaa                                                               <  2:859502/141‑1 (MQ=255)
                                                                             aTCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTaaaa                                                              >  1:682419/1‑141 (MQ=255)
                                                                              tCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTacaa                                                              >  1:407308/1‑141 (MQ=255)
                                                                               cATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAg                                                            <  1:1017634/141‑1 (MQ=255)
                                                                               cATTAAAAGAACCTGCGACGTAGGAAGAAGACAGACGTTCATCAGGTGTAATGGATGCAGAAAAACATATCGAAAAAACTGGAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTaaaaa                                                             <  2:276426/141‑1 (MQ=255)
                                                                                 ttAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAga                                                           >  1:172532/1‑141 (MQ=255)
                                                                                   aaaaGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAgatga                                                        >  1:1161600/1‑141 (MQ=255)
                                                                                    aaaGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAgatga                                                        >  1:217078/1‑141 (MQ=255)
                                                                                     aaGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGatgatg                                                      >  1:355377/1‑141 (MQ=255)
                                                                                       gAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCt                                                    <  2:787664/141‑1 (MQ=255)
                                                                                        aaCCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCt                                                    <  1:720052/141‑1 (MQ=255)
                                                                                        aaCCTGCGACTTAAAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCt                                                    <  1:298780/141‑1 (MQ=255)
                                                                                          ccTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGATAGc                                                 >  2:889020/1‑141 (MQ=255)
                                                                                          ccTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTCGCTAGTACATCTTAAAAAGATGATGCTaac                                                 >  2:342196/1‑139 (MQ=255)
                                                                                           cTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCa                                                <  1:140072/141‑1 (MQ=255)
                                                                                            tGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCa                                                >  2:303358/1‑141 (MQ=255)
                                                                                             gCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAg                                              >  2:802257/1‑141 (MQ=255)
                                                                                             gCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAg                                              <  2:904730/141‑1 (MQ=255)
                                                                                             gCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAg                                              <  1:458724/141‑1 (MQ=255)
                                                                                                aCTTATAAAGACGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAgcgc                                           <  1:252142/141‑1 (MQ=255)
                                                                                                  ttATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCt                                          <  2:407308/141‑1 (MQ=255)
                                                                                                    ataAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCatt                                        >  2:459468/1‑141 (MQ=255)
                                                                                                    ataAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCtttt                                       >  1:370321/1‑141 (MQ=255)
                                                                                                    ataAAGAAGACAGACGTTCATCAGTTGAAAGTGATGCAGAAAAACATATCGAAAAAACTGAATAACAATCTTCAGATAAAAACAAATAAGAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCttt                                        <  2:1131154/141‑1 (MQ=255)
                                                                                                      aaaGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCCAGCGCTTTTc                                      >  1:351442/1‑141 (MQ=255)
                                                                                                          aagaCAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAACGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTctatac                                 <  1:1167920/141‑1 (MQ=255)
                                                                                                          aagaCAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTctatac                                 <  1:474461/141‑1 (MQ=255)
                                                                                                              cagacGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTctatactat                              <  2:845668/141‑1 (MQ=255)
                                                                                                              cagacGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTctatactata                             >  2:106024/1‑141 (MQ=255)
                                                                                                              cagacGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTGGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTctatactata                             >  1:588810/1‑141 (MQ=255)
                                                                                                                  cGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGTTAAAAACAAATAATAACAAAGCGCCTGCTAGTACCACTTAAAAAGATGATGCTAGCAAGGGCTTTTCTATACTATAtatt                         >  2:442408/1‑141 (MQ=255)
                                                                                                                   gTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATAtatta                        >  1:967262/1‑141 (MQ=255)
                                                                                                                    ttCATCAGTTGTAATCGATGCAAAAAAATGATCGAAAAAACTGAAGAACAAGCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTCTACTATAtattat                       >  2:108618/1‑141 (MQ=255)
                                                                                                                     tcatcaGTTGTAATTGATGCAGAAAAACATATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATAtattat                       >  1:509156/1‑141 (MQ=255)
                                                                                                                     tcatcaGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATattatt                      >  1:581586/1‑141 (MQ=255)
                                                                                                                      catcaGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCGTGCTAGTACCTCTTAAAAAGATACTGCTCGCAAGCGCTTTTCTATACTATATATTAttt                     >  1:1069352/1‑141 (MQ=255)
                                                                                                                       atcaGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCCAGCGCTTTTCTATACTATATATTAtttt                    <  1:240033/141‑1 (MQ=255)
                                                                                                                       atcaGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTAtttt                    <  1:919394/141‑1 (MQ=255)
                                                                                                                       atcaGTTGTAATTGATGCAAAAAAATTATCGAAAAAAATGAAGAACAATCTTAAGATACAACCAAATAACCATCAACCGCCTGATCGAACCCCTTAAAAAGATGATGCTAACAAGCGCGTTTCTATACTATATATTAtttt                    >  2:694256/1‑141 (MQ=255)
                                                                                                                        tcaGTTGTAATTGATGCAGAAAAACATATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATattttatttt                    >  2:220004/1‑141 (MQ=255)
                                                                                                                        tcaGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAACGCTTTTCTATACTATATATTAttttt                   >  2:86858/1‑141 (MQ=255)
                                                                                                                          aGTTGTAATTGATGCAGAAAAACATATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTCTAATATATATTATTTTTc                  >  1:447077/1‑141 (MQ=255)
                                                                                                                          aGTTGTAATTGATGCAAAAAAATTATCGAAAAACCTGGAGAAGAATGTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCt                 <  1:1118386/141‑1 (MQ=255)
                                                                                                                            ttGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAACGCGCATTCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTaa               <  2:1025322/141‑1 (MQ=255)
                                                                                                                            ttGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTaa               <  1:950414/141‑1 (MQ=255)
                                                                                                                                aaTTGATGCAGAAAAACATATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCGTTTCTATCCTATATATTATTGTTCTAAAAt            >  1:531429/1‑141 (MQ=255)
                                                                                                                                aaTTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTCTACTATATATTATTTTTCTAAAAtt           >  2:560539/1‑141 (MQ=255)
                                                                                                                                 aTTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAAttt          <  1:222399/141‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                            
GAATCAGCAGAATCAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAATTTTAACACCC  >  CP000730/2455554‑2455832

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: