Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	396,540	G→A	25.9%	A208T (GCA→ACA)	USA300HOU_0364 →	possible lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	396,540	0	G	A	25.9%	140.3 / 44.6	88	A208T (GCA→ACA)	USA300HOU_0364	possible lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (36/28);  new base A (15/8);  total (51/37)
Fisher's exact test for biased strand distribution p-value = 6.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

ATACATTAGATATCATACCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAG‑ATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAACGGCGGTTATGAGTCA  >  CP000730/396400‑396677                                                                                                                                             |                                                                                                                                           aTACATTAGATATCATACCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGgcg                                                                                                                                            >  1:423695/1‑141 (MQ=255)  tACATTAGATATCATACCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGgcgc                                                                                                                                           >  1:603482/1‑141 (MQ=255)  tACATTAGATATCATACAAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGgcgc                                                                                                                                           >  1:72616/1‑141 (MQ=255)          atatCATACCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATCCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCACaaaaaa                                                                                                                                   <  1:926515/141‑1 (MQ=255)           tatCATACCAAAAGTAATGCTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAAt                                                                                                                                  <  2:465371/141‑1 (MQ=255)            ataaTACCAAAAATAAGGTTACAAGGTTCTGTTGACCTATTATATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAAtt                                                                                                                                 <  1:1145986/138‑1 (MQ=255)            atCATACCAAAATTAATGTTACAAGGGTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAAtt                                                                                                                                 >  2:718263/1‑141 (MQ=255)               aTACCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTAt                                                                                                                              >  1:988921/1‑141 (MQ=255)               aTACCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTACAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTAt                                                                                                                              >  1:424007/1‑141 (MQ=255)                 aCCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGa                                                                                                                            >  1:96312/1‑141 (MQ=255)                 aCCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGa                                                                                                                            <  1:820555/141‑1 (MQ=255)                 aCCAAAATTAATGTTACAAGGTTCTGTGGACATATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAGGCAGAAATTTATTCACGTACAGATTTATATGATTTTAAAGCGAAGGTTGAAGGCGGGGAAAAAATTTATGa                                                                                                                            >  1:424531/1‑141 (MQ=255)                   cAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACt                                                                                                                          <  1:998183/141‑1 (MQ=255)                          aaTGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTaa                                                                                                                   >  2:1164081/1‑141 (MQ=255)                            ttttACAAGGGTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGCTTTCATTGGATAATGAGAAGGCGCACAAAAAATTTATGACTTATTTAAAc                                                                                                                 >  2:539980/3‑141 (MQ=255)                            tGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAAc                                                                                                                 >  1:682896/1‑141 (MQ=255)                            tGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAAc                                                                                                                 >  1:230818/1‑141 (MQ=255)                             gTTACGAGGTGCTGCTGACATATTAAATTAAGTTGCAACTTCTAAAATCCTAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAAcc                                                                                                                <  2:681977/141‑1 (MQ=255)                             gTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGGGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAAcc                                                                                                                >  1:796042/1‑141 (MQ=255)                                    ggTTCTGTTGACCTATGAACTGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTa                                                                                                         <  2:977671/141‑1 (MQ=255)                                       tCTTTTGACCTATTAACTGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGACATTTATTCACATACAGATTAATATGCTTTTAAAGCGCACGTTCAAGGCACACAAAAAATTTCTGACTTATTTAAACCTATTTTagag                                                                                                      >  2:204624/1‑141 (MQ=255)                                        cTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAgaga                                                                                                     >  1:310465/1‑141 (MQ=255)                                           ttGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGaaaa                                                                                                  >  2:388278/1‑141 (MQ=255)                                           ttGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGaaaa                                                                                                  >  1:702514/1‑141 (MQ=255)                                               ccTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAG‑a                                                                                             <  2:72616/141‑1 (MQ=255)                                               ccTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAG‑a                                                                                             >  2:616188/1‑141 (MQ=255)                                               ccTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATGTATATGCTTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAG‑a                                                                                             >  1:1178775/1‑141 (MQ=255)                                                   ttAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAG‑ATaaa                                                                                         >  2:1091644/1‑141 (MQ=255)                                                       aTGAAGTAGCAACTACTAAAAAAACAAGAGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAAAATAAAAGAAAAACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAG‑ATAAAAAAt                                                                                     <  2:962357/141‑1 (MQ=255)                                                         gAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAG‑ATAAAAAATTa                                                                                   >  2:765122/1‑141 (MQ=255)                                                          aaGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAG‑ATAAAAAATTaa                                                                                  >  1:712091/1‑141 (MQ=255)                                                           aGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAG‑ATAAAAAATTAAg                                                                                 >  1:454187/1‑141 (MQ=255)                                                           aGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAG‑ATAAAAAATTAAg                                                                                 >  1:605414/1‑141 (MQ=255)                                                            ggtGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGTCGGTTCAGAATTAATTTAGTACTTATTTCAGGGGTTTTTAGAGAAGAAAG‑TTAAAAAATTAAGt                                                                                <  2:543882/139‑1 (MQ=255)                                                            gTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAG‑ATAAAAAATTAAGt                                                                                <  1:1004287/141‑1 (MQ=255)                                                               gCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAG‑ATAAAAAATTAAGtgat                                                                             <  1:188791/141‑1 (MQ=255)                                                                cAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAG‑ATAAAAAATTAAGtgatg                                                                            <  1:1081188/141‑1 (MQ=255)                                                                 aaCTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGAACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAG‑ATAAAAAATTAAGtgatga                                                                           <  1:333082/141‑1 (MQ=255)                                                                  aCTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAG‑ATAAAAAATTAAGTgatgat                                                                          >  1:960774/1‑141 (MQ=255)                                                                    ttCTAAACACACAGGAGTAGAAGAAATTTATTCACATACAGATTTATAGCCTTTGAAAGTGAACGTTGAAGGGGGCCAAAACATGTAGGACTTATTGAAGAATATTGTAGAGAAAAAAG‑ATAAAAAATTAAGGACTGatat                                                                        >  2:314237/1‑141 (MQ=255)                                                                    ttCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAG‑ATAAAAAATTAAGTGATGatat                                                                        >  1:134857/1‑141 (MQ=255)                                                                     tCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGCCTTATTTAAAACTATTTTAGAGAAAAAAG‑ATAAAAAAATAAGTGATGatata                                                                       >  2:415265/1‑140 (MQ=255)                                                                     tCTAAAATCACAGGCGAAGAAGAAATTTATTCACATACAGATTCATATGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAG‑ATAAAAAATTAAGTGATGATATc                                                                       <  2:616718/141‑1 (MQ=255)                                                                      cTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAG‑ATAAAAAATTAAGTGATGATATcc                                                                      >  2:154066/1‑141 (MQ=255)                                                                       tAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAG‑ATAAAAAATTAAGTGATGATATCCa                                                                     <  2:230818/141‑1 (MQ=255)                                                                        aaaaTCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCACACAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAG‑GTAAAACATTAATTGATGATATCCaa                                                                    >  1:324750/1‑141 (MQ=255)                                                                          aaTCACAGGTGAAGAAGAAATTTATACACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCAC‑AAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAG‑ATAAAAAATTAAGTGATGATATCCAAATg                                                                 >  1:655792/1‑141 (MQ=255)                                                                           aTCAAAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACAAAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAG‑ATAAAAAATTAAGTGATGATATCCAAATg                                                                 <  2:600765/141‑1 (MQ=255)                                                                            tCACAGGTGAAGAAGAAATTTATTCACAAACAGATTCATATGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAG‑ATAAAAAATTAAGTGACGATATCCAAATGa                                                                >  2:589413/1‑141 (MQ=255)                                                                              acaGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAG‑ATAAAAAATTAAGTGATGATATCCAAATGAAc                                                              >  2:440920/1‑141 (MQ=255)                                                                              acaGGTCAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCAATAATAGAGAAAAAAG‑ATAAAAAATTAAGTGATGATATCCAAATGAAc                                                              <  2:674285/141‑1 (MQ=255)                                                                              acaGGCGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAG‑ATAAAAAATTAAGTGATGATATCCAAATGAAc                                                              <  1:248453/141‑1 (MQ=255)                                                                               caGGTGAAGAAGAAATTTATTCACATACAGATTTATGTGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAG‑ATAAAAAATTAAGTGATGATATCCAAATGAACt                                                             <  1:663215/141‑1 (MQ=255)                                                                                aGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTCCATTAATCCTATTTTAGAGAAAAAAG‑ATAAAAAATTAAGTGATGATATCCAAATGAACtt                                                            >  1:274528/1‑141 (MQ=255)                                                                                aGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAG‑ATAAAAAATTAAGTGATGATATCCAAATGAACtt                                                            >  1:1136221/1‑141 (MQ=255)                                                                                aGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTACTTGAGAGAAAACAG‑ATAAAAAATTAAGTGATGATATCCAAATGAACtt                                                            >  1:865765/1‑141 (MQ=255)                                                                                aGGTGAAGAAGAAATTTATTCACATACAGATTTATATGACTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTGAAACCTATTTTAGAGAAAAAAG‑ATAAAAAATCAAATGATGATATCCAAATGAACtc                                                            >  2:409839/1‑140 (MQ=255)                                                                                     aagaagaaATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATGCGACTCGTAAAAG‑ATACAAAATTCAGTGATGATATCCAAATGAACTTCGATa                                                       >  1:1171850/1‑141 (MQ=255)                                                                                     aagaagaaATTTATTCACACACAGATTTATATGATTTTAAAGCGAACGTTGCAGGCACACAAAAAATTTATAACTTATTCAAACCTATTTCAGAAAAAAAAG‑ACAAAAAATTAAGTGATGATATCCAAAAGAACGTCGATa                                                       >  2:1008283/1‑141 (MQ=255)                                                                                     aagaagaaAGTTATTCACATACAGATTTATATGATTTTAAAGCTAACGTTGAAGGCGCACAAAAAAATTATGACTTATTTAAACCTATTTTAGAGAAAAAAG‑ATAAAAAATTAAGTGATGAGATCCAAATGAACTTCGATa                                                       <  2:44659/141‑1 (MQ=255)                                                                                      agaagaaATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAG‑ATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATaa                                                      >  2:841407/1‑141 (MQ=255)                                                                                       aaagaaATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCAACCAAAATCTATGACTTATTTAAACCTATTTTAGAGAAAAAAG‑ATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATaaa                                                     <  1:568446/140‑1 (MQ=255)                                                                                          aaaaTTTATTCACATACAGATTTATATGATTTTAAAGAGAACGTTGAAGTTGCACAAAAAATTTATTAATTATTTAAACCTATTTTATAGAAAAAAG‑ATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTg                                                  <  2:486148/140‑1 (MQ=255)                                                                                           aaaTTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATATTAGAAAAAAAAG‑ATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGa                                                 >  2:1023319/1‑141 (MQ=255)                                                                                              tttATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAG‑ATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATc                                              >  1:378380/1‑141 (MQ=255)                                                                                                   tCACATACAGATCTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAATCTATTTTAGAGAAAAAAG‑ATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAatta                                         <  2:1090177/141‑1 (MQ=255)                                                                                                    cacaTACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAG‑ATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAattat                                        <  1:575511/141‑1 (MQ=255)                                                                                                    cacaTACAGATTTATATGATTTTAAAGCGAACATTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAG‑ATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAattat                                        <  2:733452/141‑1 (MQ=255)                                                                                                        tACAGATTTATATGATTTTAAAGCGAACGTTTAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAG‑ATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGa                                    <  1:150267/141‑1 (MQ=255)                                                                                                            gATTTATATGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAG‑ATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATaaa                                >  2:671059/1‑141 (MQ=255)                                                                                                             aTTTATATGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAG‑ATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAat                               <  2:423695/141‑1 (MQ=255)                                                                                                                tataTGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAG‑ATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAAtata                            >  2:369984/1‑141 (MQ=255)                                                                                                                   atGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAG‑ATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAg                         >  2:745092/1‑141 (MQ=255)                                                                                                                     gATTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAG‑ATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGAt                       <  2:274528/141‑1 (MQ=255)                                                                                                                      aTTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAG‑ATAAAAAATTAAGTGATGATCTCCAAAGGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATa                      >  1:270840/1‑141 (MQ=255)                                                                                                                      aTTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAG‑ATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATa                      >  2:487504/1‑141 (MQ=255)                                                                                                                      aTTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAG‑ATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATa                      >  1:916571/1‑141 (MQ=255)                                                                                                                      aTTGTAAAGCGAACGTGGAAGGCGCACAAAAAATTTATGACTTTTTTAAACCTACTTTAGAGAAAAAAG‑ATAAAAAATTAATTGATGATATCCAAATGAACTTTGATAAAGTGAATCAATTATTGGATAAATATAAAGATa                      <  2:1183968/141‑1 (MQ=255)                                                                                                                        tttAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAG‑ATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATaac                    <  1:371368/141‑1 (MQ=255)                                                                                                                        tttAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAG‑ATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGCGAATCAATTATTGGATAAATATAAAGATaac                    >  2:653846/1‑141 (MQ=255)                                                                                                                             aGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAG‑ATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAAagg               >  2:692617/1‑141 (MQ=255)                                                                                                                             aGCGAACGTAAAAGGAGCAAAAAAAAAACATCATTTATACAAACAAATTTCACACAAAAAAG‑ATAAAAAATCAAGTGATGATCTCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAAcgg               <  1:539980/141‑1 (MQ=255)                                                                                                                               cGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAG‑ATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAAcggcg             >  2:914492/1‑141 (MQ=255)                                                                                                                                 aaagTTGAAAGCAAAAAAAAAATTTATGACCTATTTAAACCTATTTTAAAGAAAAAAGAAAAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAAcggcgg            <  1:1164081/138‑1 (MQ=255)                                                                                                                                   cGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAG‑ATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAACGGCGGTTa         <  2:157781/141‑1 (MQ=255)                                                                                                                                   cGTTGAAGGCAAACAAAAAATTTATAACTTATTTAAACCTATTTGAGAGAAAAAAA‑AAAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAACGGCGGTTa         <  2:1062327/141‑1 (MQ=255)                                                                                                                                     ttGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAG‑ATACAAAATTAAGTGATGATATCCAAATGAACTTCGAAAAAGTGAATCAATTATTGGATAAATATAAAGATAACAACGGCGGTTATg       >  2:393842/1‑141 (MQ=255)                                                                                                                                       gAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAG‑ATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAGATATAAAGATAACAACGGCGGttgttag     >  1:76725/1‑136 (MQ=255)                                                                                                                                       gAAGGCGCACAAAAAATTTATGACTTATTTAAACCGATTTTAGAGAAAAAAG‑ATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAACGGCGGTTATGAg     >  1:1123627/1‑141 (MQ=255)                                                                                                                                          ggCGCACAAAAACTTTATGACTTATTTAACCCCATTTAAGAGAAAAAAG‑ATAAAAAATTAAGTGATGATATCCAAATGAACCTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAACGGCGGTTATGAGTCa  <  1:314237/141‑1 (MQ=255)                                                                                                                                          ggCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAG‑ATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATGATTGGATAAATATAAAGATAACAACGGCGGTTATGAGTCa  <  2:134857/141‑1 (MQ=255)                                                                                                                                             |                                                                                                                                           ATACATTAGATATCATACCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAG‑ATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAACGGCGGTTATGAGTCA  >  CP000730/396400‑396677

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑