Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 442,222 A→G 5.6% intergenic (‑290/+103) USA300HOU_0415 ← / ← USA300HOU_0416 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730442,2220AG 5.6% 378.1 / 9.7 125intergenic (‑290/+103)USA300HOU_0415/USA300HOU_0416hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (68/49);  new base G (5/2);  total (73/52)
Fisher's exact test for biased strand distribution p-value = 6.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.91e-01

Only 100 of 130 total aligned reads displayed.

CGATAATAGTGACATGTGGCTTATAACACATTGTGTAATTTATTGTGTGTTTTGTGATGTCTAATATTGATTGCAGATTGTTGTATATTTCACTTAAAGGGCATTTATATAAAAAAAGAGGCCAGATGACGTATCATCTGACCTTCAGCTAAAACGCTGAGAATATGTCATTCTTCAAGAGAATGAAGCCGGAATGGTTACCGGGAATTTGTAAATCAATTATATCAGATGTAATATTACAATTCAATTAGAAAAGAACTTTATCTTTAATTGCTTTTCGA  >  CP000730/442082‑442362
                                                                                                                                            |                                                                                                                                            
cGATAATAGTGACATGTGGCGTATAACACATTGTGTAATTTATTGTGTGTTTTGTGATGTCTAATATTGATTGCAGATTGTTGTATATTTCACTTAAAGGGCATTTATATAAAAAAAGAGGCCAGATGACGTATCATCTGa                                                                                                                                              <  1:394077/141‑1 (MQ=255)
    aataGTGACATGTGGCTTATAACACATTGTGTAATTTATTGTGTGTTTTGTGATGTCTAATATTGATTGCAGATTGTTGTATATTTCACTTAAAGGGCATTTATATAAAAAAAGAGGCCAGATGACGTATCATCTGACCtt                                                                                                                                          <  1:87421/141‑1 (MQ=255)
         tGACATGTGGCTTATAACACATTGTGTAATTTATTGTGTGTTTTGTGATGTCTAATATTGATTGCAGATTGTTGTATATTTCACTTAAAGGGCATTTATATAAAAAAAGAGGCCAGATGACGTATCGTCTGGCCTTGAGCt                                                                                                                                     <  2:1161750/141‑1 (MQ=255)
          gACATGTGGCTTATAACACATTGAGTAATTTATTGTGTGTTTTGTGATGTCTAATATTGATTGCAGATTGTTGTATATTTCACTTAAAGGGCATTTATATAAAAAAAGAGGCCAGATGACGTATCATCTGACCTTCAGCTa                                                                                                                                    >  2:139998/1‑141 (MQ=255)
             aTGTGGCTTATAACACATTGTGTAATTTATTGTGTGTTTTGTGATGTCTAATATTGATTGCAGATTGTTGTATATTTCACTTAAAGGGCATTTATATAAAAAAAGAGGCCAGATGACGTATCATCTGACCTTCAGCTaaaa                                                                                                                                 <  1:174577/141‑1 (MQ=255)
             aTGTGGCTTATAACACATTGTGTAATTTATTGTGTGTTTTGTGATGTCTAATATTGATTGCAGATTGTTGTATATTTCACTTAAAGGGCATTTATATAAAAAAAGAGGCCAGATGACGTATCATCTGACCTTCAGCTaaaa                                                                                                                                 <  1:487200/141‑1 (MQ=255)
              tgtgGCTTATAACACATTGTGTAATTTATTGTGTGTTTTGTGATGTCTAATATTGATTGCAGATTGTTGTATATTTCACTTAAAGGGCATTTATATAAAAAAAGAGGCCAGATGACGTATCATCTGACCTTCAGCTAAAAc                                                                                                                                >  1:610050/1‑141 (MQ=255)
                  gCTTATAACACATTGTGTAATTTATTGTGTGTTTTGTGATGTCTAATATTGATTATAGAGTGTTGTATATTTCACTTAAAGGGCATTTATATAAAAAAAGAGGCCAGATGACGTATCATCTGACCTTCAACTAAAACGCTg                                                                                                                            >  1:795990/1‑141 (MQ=255)
                   cTTATAACACATTGTTGAATTTGTTGTGTGTTTTGTGATGTTTAATATTGATTGCAGATTGTTGTATATTTCACTTAAAGGGCATTTATATAAAAAAAGAGGCCAGATGACGTATCATCTGACCTTCAGCTAAAACGCTga                                                                                                                           <  1:683818/141‑1 (MQ=255)
                         acacATTGTGTAATTTATTGTGTGTTTTGTGATGTCTAATATTGATTGCAGATTGTTGTATATTTCACTTAAAGGGCATTTATATAAAAAAAGAGGCCAGATGACGTATCATCTGACCTTCAGCTAAAACGCTGAGAatat                                                                                                                     >  1:442850/1‑141 (MQ=255)
                             aTTGTGTAATTTATTGTGTGTTTTGTGATGTCTAATATTGATTGCAGATTGTTGTATATTTCACTTAAAGGGCATTTATATAAAAAAAGAGGCCAGATGACGTATCATCTGACCTTCAGCTAAAACGCTGAGAATATGTCa                                                                                                                 >  1:607267/1‑141 (MQ=255)
                              ttGTGTAATTTATTGTGTGTTTTGTGATGTCTAATATTGATTGCAGATTGTTGTATATTTCACTTAAAGGGCATTTATATAAAAAAAGAGGCCAGATGACGTATCATCTGACCTTCAGCTAAAACGCTGAGAATATGTCAt                                                                                                                <  1:1112759/141‑1 (MQ=255)
                                gtgtAATTTATTGTGTGTTTTGTGATGTCTAATATTGATTGCAGATTGTTGTATATTTCACTTAAAGGGCATTTATATAAAAAAAGAGGCCAGATGACGTATCATCTGACCTTCAGCTAAAACGCTGAGAATATGTCAttc                                                                                                              >  2:322876/1‑141 (MQ=255)
                                gtgtAATTTATTGTGTGTTTTGTGATGTCTAATATTGATTGCAGATTGTTGTATATTTCACTTAAAGGGCATTTATATAAAAAAAGAGGCCAGATGACGTATCATCTGACCTTCAGCTAAAACGCTGAGAATATGTCAttc                                                                                                              >  2:1012136/1‑141 (MQ=255)
                                 tgtAATTTATTGTGTGTTTTGTGATGTCTAATATTGATTGCAGATTGTTGTATATTTCACTTAAAGGGCATTTATATAAAAAAAGAGGCCAGATGACGTATCATCTGACCTTCAGCTAAAACGCTGAGTATATGTCAttct                                                                                                             >  1:43388/1‑141 (MQ=255)
                                 tgtAATTTATTGTGTGTTTTGTGATGTCTAATATTGATTGCAGACTGTTGTATATTTCACTTAAAGGGCATTTATATAAAAAAAGAGGCCAGATGACGTATCATCTGACCTTCAGCTAAAACGCTGAGAATATGTCAttct                                                                                                             <  1:391115/141‑1 (MQ=255)
                                   tAATTTATTGTGTGTTTTGTGATGTCTAATATTGATTGCAGATTGTTGTATATTTCACTTAAAGGGCATTTATATAAAAAAAGAGGCCAGATGACGTATCATCTGACCTTCAGCTAAAACGCTGAGAATATGTCAttcttc                                                                                                           <  1:588343/141‑1 (MQ=255)
                                   tAAGTTATTGTGTGTTTTGTGATGTCTAATATTGATTGCAGATTGTTGTATATTTCACTTAAAGGGCATTTATATAAAAAAAGAGGCCAGCTGACGTATCATCTGACCTTCAGCTAAAACGCTGAGAATATGTCAttcttc                                                                                                           <  1:1131408/141‑1 (MQ=255)
                                         tttGTGTGTTTTGTGATGTCTAATATTGATTGCAGATTGTTGTATATTTCACTTAAAGGGCATTTATATAAAAAAAGAGGCCAGATGACGTATCATCTGACCTTCAGCTAAAACGCTGAGAATATGTCATTCTTCAAgaga                                                                                                     <  2:1199787/140‑1 (MQ=255)
                                         aTTGTGTGTTTTGTGATGTCTAATATTTAATGCAGATTGTTGTATATTTCACTTAAAGGGCATTTATATAAAAAAAGAGGCCAGATGACGTATCATCTGACCTTCAGCTAAAACGCTGAGAATATGTCATTCTTCAAgaga                                                                                                     <  2:1146025/141‑1 (MQ=255)
                                         aTTGTGTGTTTTGTGATGTCTAATATTGATTGCAGATTGTTGTATATTTCACTTAAAGGGCATTTATATAAAAAAAGAGGCCAGATGACGTATCATCTGACCTTCAGCTAAAACGCTGAGAATATGTCATTCTTCAAgaga                                                                                                     <  1:476457/141‑1 (MQ=255)
                                         aTTGTGTGTTTTGTGATGTCTAATATTGATTGCAGATTGTTGTATATTTCACTTAAAGGGCATTTATATAAAAAAAGAGGCCAGATGAAGTACCACCTCACCTTCAGCTAAAACGCTGAGAATATGTCATTCTTCAAgaga                                                                                                     <  1:630219/141‑1 (MQ=255)
                                               tgtTTTGTGATGTCTAATATTGATTGCAGATTGTTGTATATTTCACTTAAAGGGCATTTATATAAAAAAAGAGGCCAGATGACGTATCATCTGACCTTCAGCTAAAACGCTGAGAATATGTCATTCTTCAAGAGAATGAAg                                                                                               >  1:1111471/1‑141 (MQ=255)
                                                   ttGTGATGTCTAATATTGATTGCAGATTGTTGTATATTTCACTTAAAGGGCATTTATATAAAAAAAGAGGCCAGATGACGTATCATCTGACCTTCAGCTAAAACGCTGAGAATATGTCATTCTTCAAGAGAATGAAGCCgg                                                                                           >  2:474093/1‑141 (MQ=255)
                                                   ttGTGATGTCTAATATTGATTGCAGATTGTTGTATATTTCACTTAAAGGGCATTTATATAAAAAAAGAGGCCAGATGACGTATCATCTGACCTTCAGCTAAAACGCTGAGAATATGTCATTCTTCAAGAGAATGAAGCCgg                                                                                           >  2:584569/1‑141 (MQ=255)
                                                   ttGTGATGTCTAATATTGATTGCAGATTGTTGTATATTTCACTTAAAGGGCATTTATATAAAAAAAGAGGCCAGATGACGTATCATCTGACCTTCAGCTAAAACGCTGAGAATATGTCATTCTTCAAGAGAATGAAACCgg                                                                                           >  2:422323/1‑141 (MQ=255)
                                                    tgtgATGTCAAATATTTATTGCAAATTGTAGTATATTTCACATAAAGGGCATTTATATAAAAAAAGAGGCCAGATGACGTATCATCTGACCTTCAGCTAAAACGCTGAGAATATGTCATTCTTCAAGAGAATGAAGCCGGa                                                                                          <  1:401890/141‑1 (MQ=255)
                                                      tgATGTCTAATATTGATTGCAGATTGTTGTATATTTCACTTAAAGGGCATTTATATAAAAAAAGAGGCCAGATGACGTATCATCTGACCTTCAGCTAAAACGCTGAGAATATGTCATTCTTCAAGAGAATGAAGCCGGAAt                                                                                        >  1:255149/1‑141 (MQ=255)
                                                      tgATGTCTAATATTGATTGCAGATTGTTGTATATTTCACTTAAAGGGCATTTATATAAAAAAAGAGGCCAGATGACGTATCATCTGACCTTCAGCTAAAACGCTGAGAATATGTCATTCTTCAAGAGAATGAAGCCGGAAt                                                                                        >  2:463266/1‑141 (MQ=255)
                                                      tgATGTCTAATATTGATTGCAGATTGTTGTATATTTCACTTAAAGGGCATTTATATAAAAAAAGAGGCCAGATGACGTATCATCTGACCTTCAGCTAAAACGCTGAGAATATGTCATTCTTCAAGAGAATGAAGCCGGAAt                                                                                        <  1:429482/141‑1 (MQ=255)
                                                      tgATGTCTAATATTGATTGCAGATTGTTGTATATTTCACTTAAAGGGCATTTATATAAAAAAAGAGGCCAGATGACGTATCATCTGACCTTCAGCTAAAACGCTGAGAATATGTCATTCTTCAAGAGAATGAAGCCGGAAt                                                                                        >  1:711918/1‑141 (MQ=255)
                                                      tgATGTCTAATATTGATTGCAGATTGTTGTATATTTCACTTAAAGGGCATTTATATAAAAAAAGAGGCCAGATGACGTATCATCTGACCTTCAGCTAAAACGCTGAGAATATGTCATTCTTCAAGAGAATGAAGCCGGAAt                                                                                        >  1:89462/1‑141 (MQ=255)
                                                      ggATGCCAAATATTGATTGTAGAGTTTTGTATTTTTCACTTAAAGGGCATTTATATAAAAAAAGAGGCCAGATGACGTATCATCTGACCTTCAGCTAAAACGCTGAGAATATGTCATTCTTCAAGAGAATGAAGCCGGAAt                                                                                        <  1:439223/140‑1 (MQ=255)
                                                        aTGTCTAATATTGATTGCAGATTGTTGTATATTTCACTTAAAGGGCATTTATATAAAAAAAGAGGCCAGATGACGTATCATCTGGCCTTCAGCTAAAACGCTGAGAATATGTCATTCTTCAAGAGAATGAAGCCGGAATgg                                                                                      <  1:1138230/141‑1 (MQ=255)
                                                        aTGTCTAATATTGATTGCAGATTGTTGCACATTTAACTTAAAGGGCATTTATATAAAAAAAGAGGCCAGATGACGTATCATCTGACCTTCAGCTAAAACGCTGAGAATATGTCATTCTTCAAGAGAATGAAGCCGGAATgg                                                                                      <  1:810030/141‑1 (MQ=255)
                                                         tGTCTAATATTGATTGCAGATTGTTGTATATTTCACTTAAAGGGCATTTATATAAAAAAAGAGGCCAGATGACGTATCATCTGACCTTCAGCTAAAACGCTGAGAATATGTCATTCTTCAAGAGAATGAAGCCGGAAGGGt                                                                                     >  2:648760/1‑141 (MQ=255)
                                                           tCTAATATTGATTGCAGATTGTTGTATATTTCACTTAAAGGGCATTTATATAAAAAAAGAGGCCAGATGACGTATCATCTGACCTTCAGCTAAAACGCTGAGAATATGTCATTCTTCAAGAGAATGAAGCCGGAATGGTTa                                                                                   <  1:916776/141‑1 (MQ=255)
                                                           tCTAATAATAATAGAAGATTGCTGTATATTTCACTTAAAGTGCATTTATAGAAAAAAAGAGTCCAGATGACGTATCATCTGACCTTCAGCTAAAACGCTGAGAATATGTCATTCTTCAAGAGAATGAAGCCGGAATGGTTa                                                                                   >  2:698190/1‑141 (MQ=255)
                                                            cTAATATTGATTGCAGATTGTTGTATATTTCACTTAAAGGGCATTTATATAAAAAAAGAGGCCAGATGACGTATCATCTGACCTTCAGCGAAAACGCTGAGAATATGTCATTCTTCAAGAGAATGAAGCCGGAATGGTTAc                                                                                  <  1:429698/141‑1 (MQ=255)
                                                             tAATATTGATTGCAGATTGTTGTATATTTCACTTAAAGGGCATTTATATAAAAAAAGAGGCCAGATGACGTATCATCTGACCTTCAGCTAAAACGCTGAGAATATGTCATTCTTCAAGAGAATGAAGCCGGAATGGTTAcc                                                                                 <  2:442850/141‑1 (MQ=255)
                                                               atatTGATTGCAGATTGTTGTATCTTCTTCAGAAGGGGCATTTATATAAAAAAAGAGGCCAGATGACGTATCATCTGACCTTCAGCTAAAACGCTGAGAATATGTCATTCTTCAAGAGAATGAAGCCGGAATGGTTACCgg                                                                               >  1:1187818/1‑141 (MQ=255)
                                                               atatTGATTGCAGATTGTTGTATATTTCACTTAAAGGGCATTTATATAAAAAAAGAGGCCAGATGACGTATCATCTGACCTTCAGCTAAAACGCTGAGAATATGTCATTCTTCAAGAGAATGCAGCCGGAATGGTTACCgg                                                                               <  1:543463/141‑1 (MQ=255)
                                                                ttctaattgCTGAGGGTTGTATATCTCACTTAAAGGGAATTTATACAAAAAAAGAGGCCAGATGACGTATCATCTGACCTTCAGCTAAAACGCTGAGAATATGTCAGTCTTCAAGAGAATGAAGCCGGAATGGTTACCggg                                                                              <  2:388305/136‑1 (MQ=255)
                                                                  ttgattgCAGATTGTTGTATATTTCACTTAAAGGGCATTTATATAAAAAAAGAGGCCAGATGACGTATCATCTGACCTTCAGCTAAAACGCTGAGAATATGTCATTCTTCAAGAGAATGAAGCCGGAATGGTTACCGGGaa                                                                            <  1:510296/141‑1 (MQ=255)
                                                                  ttgattgCAGATTGTTGTATATTTCACTTAAAGGGCATTTATATAAAAAAAGAGGCCAGATGACGTATCATCTGACCTTCAGCTAAAACGCTGAGAATATGTCATTCTTCAAGAGAATGAAGCCGGAATGGTTACCGGGaa                                                                            <  2:13851/141‑1 (MQ=255)
                                                                     tttgCAGATTTTTGTATTATTCTCTTAAAGGGCATTTATATAAAAAAAGAGGCCAGATTACGTATCATCTGACCTTCAGCTAAAACGCTGAGAATATGTCATTCTTCAAGAGAATGAAGCCGGAATGGTTACCGGGAAttt                                                                         <  2:651902/140‑1 (MQ=255)
                                                                     attgCAGATTGTTGTATATTTCACTTAAAGGGCATTTATATAAAAAAAGAGGCCAGATGACCTATCATCTGACCTTCAGCTAAAACACTGAGAATATGTCATTCTTCAAGAGCATGAAGCCGGAATGGTTACCGGGAAttt                                                                         <  1:775163/141‑1 (MQ=255)
                                                                       tgCAGATTGTTGTATATTTCACTTAAAGGGCATTTATATAAAAAAAGAGGCCAGATGACGTATCATCTGACCTTCAGCTAAAACGCTGAGAATATGTCATTCTTCAAGAGAATGAAGCCGGAATGGTTACCGGGAATTTGt                                                                       >  2:1171474/1‑141 (MQ=255)
                                                                       tgCAGATTGTTGTATATTTCACTTAAAGGGCATTTATATAAAAAAAGAGGCCAGATGACGTATCATCTGACCTTCAGCTAAAACGCTGAGAATATGTCATTCTTCAAGAGAATGAAGCCGGAATGGTTACCGGGAATTTGt                                                                       <  2:782417/141‑1 (MQ=255)
                                                                       tgCAGATTGTTGTATATTTCACTTAAAGGGAATTTATATAAAAAAAGAGGCCAGATGACGTATCATCTGACCTTCAGCTAAAACGCTGAGAATATGTCATTCTTCAAGAGAATGAAGCCGGAATGGTTACCGGGAATTTGt                                                                       >  2:728986/1‑141 (MQ=255)
                                                                        gCAGATTGTTGTATATTTCACTTAAAGGGCATTTATATAAAAAAAGAGGCCAGATGACGTATCATCTGACCTTCAGCTAAAACGCTGAGAATATGTCATTCTTCAAGAGAATGAAGCCGGAATGGTTACCGGGAATTTGTa                                                                      >  2:513460/1‑141 (MQ=255)
                                                                         cAGATTGTTGTATATTTCACTTAAAGGGCATTTCTATAAAAAAAGAGGCCAGATGACGTATCATCTGACCTTCAGCTAAAACGCTGAGAATATGTCATTCTTCAAGAGAATGAAGCCGGAATGGTTACGGGGAATTTGTaa                                                                     >  2:54951/1‑141 (MQ=255)
                                                                         cAGATTGTTGTATATTTCACTTAAAGGGCATTTATATAAAAAAAGAGGCCAGATGACGTATCATCTGACCTTCAGCTAAAACGCTGAGAATATGTCATTCTTCAAGAGAATGAAGCCGGAATGGTTACCGGGAATTTGTaa                                                                     >  2:266798/1‑141 (MQ=255)
                                                                           gATTGTTGTATATTTCACTTAAAGGGCATTTATATAAAAAAAGAGGCCAGATGACGTATCATCTGACCTTCAGCTAAAACGCTGAGAATATGTCATTCTTCAAGAGAATGAAGCCGGAATGGTTACCGGGACTTTGTAAAt                                                                   >  2:294830/1‑141 (MQ=255)
                                                                               gttgtATATTTCCCTTAAAGGGCATTGATATAAAAAAGGAGGCCAGCTCATAGAACTTCTGACCGACAGCTAAAACGCTGAGAATATGTCATTCTTCAAGAGAATGAAGCCGGAATGGTTACCGGGAATTTGTAAATCAAt                                                               <  1:1171932/141‑1 (MQ=255)
                                                                               gttgtAGATGTCACGTAAAAGGCATTTACATAAAAAAAGATGCCAGATGACGTATCATCTGACCTTCAGCTAAAACGCTGAGAATATGTCATTCTTCAAGAGAATGAAGCCGGAATGGTTACCGGGAATTTGTAAATCAAt                                                               <  2:331415/141‑1 (MQ=255)
                                                                                ttgtATATTTCACTTAAAGGGCATTTATATAAAAAAAGAGGCCAGATGACGTATCATCTGACCTTCAGCTAAAACGCTGAGAATATGTCATTCTTCAAGAGAATGAAGCCGGAATGGTTACCGGGAATTTGTAAATCAAtt                                                              >  1:932880/1‑141 (MQ=255)
                                                                                ttgtATATTTCACTTAAAGGGCATTTATATAAAAAAAGAGGCCAGATGACGTATCATCTGACCTTCAGCTAAAACGCTGAGAATATGTCATTCTTCAAGAGAATGAAGCCGGAATGGTTACCGGGAATTTGTAAATCAAtt                                                              >  1:1048952/1‑141 (MQ=255)
                                                                                ttgtATATTTCACTTAAAGGGCATTTATATAAAAAAAGAGGCCAGATGACGTATCATCTGACCTTCAGCTAAAACGCTGAGAATATGTCATTCTTCAAGAGAATGAAGCCGGAATGGTTACCGGGAATTTGTAAATCAAtt                                                              >  1:757125/1‑141 (MQ=255)
                                                                                ttgtATATTTCACTTAAAGGGCATCTATATAAAAAAAGAGGCCAGATGACGTATCATCTGACCTTCAGCTAAACCGCTGAGAATATGTCATTCTTCAAGAGAATGAAGCCGGAATGGTAACCGGGAATTTGTAAATCAAtt                                                              >  2:571534/1‑141 (MQ=255)
                                                                                 tgtATCGTTCACTTAAAGGGAATTTATATAAAAAAAGAGGCCAGATGCCGTATCACCTGACCTTCAGCTAAAACGCTGAGAATATGTCATTCTTCAAGAGAATGAAGCCGGCATGGTTACCGGGAATTTGTAAATCAATta                                                             <  1:44361/141‑1 (MQ=255)
                                                                                 tgtATATTTCACGTAAAGGGCATTTATATAAAAAAAGAGGCCAGATGACGTAGCATCTGACCTTCAGCTAAAACGCTGAGAATATGGCATTCTTCAAGAGAATGAAGCCGGAATGGTCACCGGGAATTTGTAAATCAATta                                                             <  2:856738/141‑1 (MQ=255)
                                                                                       tttCACTCAAAGGTCATTTATATAAAAAAAGAGTCCAGATGCCTTATCATCTCCCCTTCAGCTAAACCCCTGAGAATATCTCATTCTTCAAGAGAATGAAGCCTGAATGGTTACCGTGAATTTCTAAATCAATTATATCAg                                                       <  2:391768/141‑1 (MQ=255)
                                                                                        ttaaCTTAAAGGGCATTTATATAAAAAAAGAGGCCAGATGACGTATCATCTGACCTTCAGCTAAAACGCTGAGAATATGTCATTCTTCAAGAGAATGAAGCCGGAATGGTTACCGGGAATTTGTAAATCAATTATATCAGa                                                      <  2:968123/138‑1 (MQ=255)
                                                                                        ttCACTTAAAGGGCATTTATATAAAAAAAGAGGCCAGGTGACGTATCATCTGACCTTCAGCTAAAACGCTGAGAATATGTCATTCTTCAAGAGAATGAAGCCGGAATGGTTACCGGGAATTTGTAAATCAATTATATCAGa                                                      <  2:968506/141‑1 (MQ=255)
                                                                                         tCACTTAAAGGGCATTTATATAAAAAAAGAGGCCAGATGACGTATCATCTGACCTTCAGCTAAAACGCTGAGAATATGTCATTCTTCAAGAGAATGAAGCCGGAATGGTTACCGGGAATTTGTAAATCAATTATATCAGAt                                                     >  1:1059367/1‑141 (MQ=255)
                                                                                         tCACTTAAAGGGCATTTATATAAAAAAAGAGGCCAGATGACGTATCATCTGACCTTCAGCTAAAACGCTGAGAATATGTCATTCTTCAAGAGAATGAAGCCGGAATGGTTACCGGGAATTTGTAAATCAATTATATCAGAt                                                     <  1:325016/141‑1 (MQ=255)
                                                                                         tCACTTAAAGGGCATTTATATAAAAAAAGAGGCCAGATGACGTATCATCTGACCTTCAGCTAAAACGCTGAGAATATGTCATTCTTCAAGAGAATGAAGCCGGAATGGTTACCGGGAATTTGTAAATCAATTATATCAGAt                                                     >  2:510480/1‑141 (MQ=255)
                                                                                          cACTTAAAGGGCATTTATATAAAAAAAGAGGCCAGATGACGTATCATCTGACCTTCAGCTAAAACGCTGAGAATATGTCATTCTTCAAGAGAATGAAGCCGGAATGGTTACCGGGAATTTGTAAATCAATTATATCAGATg                                                    >  2:1005124/1‑141 (MQ=255)
                                                                                           aCTTAAAGGGCATTTATATAAAAAAAGAGGCCAGATGACGTATCATCTGACCTTGAGCTAAAACGCTGAGAATATGTCATTCTTCAAGAGAATGAAGCCGGAATGGTTACCGGGAATTTGTAAATCAATTATATCAGATGt                                                   >  1:798459/1‑141 (MQ=255)
                                                                                           aCTTAAAGGGCATTTATATAAAAAAAGAGGCCAGATGACGTATCATCTGACCTTCAGCTAAAACGCTGAGAATATGTCATTCTTCAAGAGAATGAAGCCGGAATGGTTACCGGGAATTTGTAAATCAATTATATCAGATGt                                                   >  1:484100/1‑141 (MQ=255)
                                                                                           aCTTAAAGGGCATTTATATAAAAAAAGAGGCCAGATGACGTATCATCTGACCTTCAGCTAAAACGCTGAGAATATGTCATTCTTCAAGAGAATGAAGCCGGAATGGTTACCGGGAATTTGTAAATCAATTATATCAGATGt                                                   >  2:936228/1‑141 (MQ=255)
                                                                                           aCTTAAAGGGCATTTATATAAAAAAAGAGGCCAGATGACGTATCATCTGACCTTCAGCTAAAACGCTGAGAATATGTCATTCTTCAAGAGAATGAAGCCGGAATGGTTACCGGGAATTTGTAAATCAATTATATCAGATGt                                                   <  1:417235/141‑1 (MQ=255)
                                                                                           aCTTAAAGGGCATTTATATAAAAAAAGAGGCCAGATGACGTATCATCTGACCTTCAGCTAAAACGCTGAGAATATGTCATTCTTCAAGAGAATGAAGCCGGAATGGTTACCGGGAATTTGTAAATCAATTATATCAGATGt                                                   >  1:346972/1‑141 (MQ=255)
                                                                                            cTTAAAGGGCATTTATATAAAAAAAGAGGCCAGATGACGTATCATCTGACCTTCAGCTAAAACGCTGAGAATATGTCATTCTTCAAGAGAATGAAGCCGGAATGGTTACCGGGAATTTGTAAATCAATTATATCAGATGTa                                                  <  1:154305/141‑1 (MQ=255)
                                                                                            cTTAAAGGGCATTTATATAAAAAAAGAGGCCAGATGACGTATCATCTGACCTTCAGCTAAAACGCTGAGAATATGTCATTCTTCAAGAGAATGAAGCCGGAATGGTTACCGGGAATTTGTAAATCAATTATATCAGATGTa                                                  >  1:1102270/1‑141 (MQ=255)
                                                                                               aaaGGGCATTTATATAAAAAAAGAGGCCAGATGACGTATCATCTGACCTTCAGCTAAAACGCTGAGAATATGTCATTCTTCAAGAGAATGAAGCCGGAATGGTTACCGGGAATTTGTAAATCAATTATATCAGATGTAata                                               >  1:990238/1‑141 (MQ=255)
                                                                                                aaGGGCATTTATATCAAAAAAGAGGCCAGATGACGTATCATCTGACCTTCAGCTAAAACGCTGAGAATATGTCATTCCTCAAGAGAATGAGGCCGGAATGGTTACCGGGAATTTGTAAATCAATTATATCAGATGTAatat                                              >  2:819291/1‑141 (MQ=255)
                                                                                                  gggCATTTATATAAAAAAAGAGGCCAGATGACGTATCATCTGACCTTCAGCTAAAACGCTGAGAATATGTCATTCTTCAAGAGAATGAAGCCGGAATGGTTACCGGGAATTTGTAAATCAATTATATCAGATGTAATATTa                                            >  2:972349/1‑141 (MQ=255)
                                                                                                  gggCATTTATATAAAAAAAGAGGCCAGATGACGTATCATCTGACCTTCAGCTAAAACGCTGAGAATATGTCATTCTTCAAGAGAATGAAGCCGGAATGGTTACCGGGAATTTGTAAATCAATTATATCAGATGTAATATTa                                            >  2:941592/1‑141 (MQ=255)
                                                                                                  gggCATTTATATAAAAAAAGAGGCCAGATGACGTATCATCTGACCTTCAGCTAAAACGCTGAGAATATGTCATTCTTCAAGAGAATGAAGCCGGAATGGTTACCGGGAATTTGTAAATCAATTATATCAGATGTAATATTa                                            >  2:290295/1‑141 (MQ=255)
                                                                                                  gggCATTTATATAAAAAAAGAGGCCAGATGACGTATCATCTGACCTTCAGCTAAAACGCTGAGAATACGTCATTCTTCAAGAGAATGAAGCCGGAATGGTTACCGGGAATTTGTAAATCAATTATATCAGATGTAATATTa                                            <  1:233329/141‑1 (MQ=255)
                                                                                                         tatataAAAAAAGAGGCCAGATGACGTATCATCTGACCTTCAGCTAAAACGCTGAGAATATGTCATTCTTCAAGAGAATGAAGCCGGAATGGTTACCGGGAATTTGTAAATCAATTATAGCAGATGTAATATTAcaattca                                     >  2:155357/1‑141 (MQ=255)
                                                                                                             taAAAAAAGAGGCCAGATGACGTATCATCTGACCTTCAGCTAAAACGCTGAGAATATGTCATTCTTCAAGAGAATGAAGCCGGAATGGTTACCGGGAATTTGTAAATCAATTATATCAGATGTAATATTACAATTCAATTa                                 <  2:341142/141‑1 (MQ=255)
                                                                                                             taAAAAAAGAGGCCAGAGGACGTATCATCTGACCTTCAGCTAAAACGCTGAGAATATGTCATTCTTCAAGAGAATGAAGCCGGAATGGTTACCGGGAATTTGTAAATCAATTATATCAGATGTAATATTACAATTCAATTa                                 <  1:1046912/141‑1 (MQ=255)
                                                                                                               aaaaacGAGGCCCAATGACGTATCATCTGACCTTCACCTAAAACGCTGAGAATATGTCCTTCTTCAAGAGAATGAAGCCGGCATGGTTACCGGGAATTTGTCAATCAATTATATCAGATGTAATATTACAATTCAATTAGa                               <  1:553850/141‑1 (MQ=255)
                                                                                                                   aaGAGGCCAGACTACGTATCATATGACCTTCAGCGAAACCGCTGAGAATATGTCATTGTTCAAGAGAATGAAGCCGGAATGGTTACCGTGAATTTGTAAATCAATTATATCAGATGTAATATTACAATTCAATTAGAAAAg                           <  2:66903/141‑1 (MQ=255)
                                                                                                                    agagGCCAGATGACGTATCATCTGACCTTCAGCTAAAACGCTGAGAATATGTCATTCTTCAAGAGAATGAAGCCGGAATGGTTACCGGGAATTTGTAAATCAATTATATCAGATGTAATATTACAATTCCATTAGAAAAGa                          >  2:215696/1‑141 (MQ=255)
                                                                                                                    agagGCCAGATGACGTATCATCTGACCTTCAGCTAAAACGCTGAGAATATGTCATTCTTCAAGAGAATGAAGCCGGAATGGTTACCGGGAATTTGTAAATCAATTATATCAGATGTAATATTACAATTCAATTATAAAAGa                          >  1:262829/1‑141 (MQ=255)
                                                                                                                    agagGCCAGATGACGTATCATCTGACCTTCAGCTAAAACGCTGAGAATATGTCATTCTTCAAGAGAATGAAGCCGGAATGGTTACCGGGAATTTGTAAATCAATTATATCAGATGTAATATTACAATTCAATTAGAAAAGa                          >  1:1125745/1‑141 (MQ=255)
                                                                                                                    agagGCCAGATGACGTATCATCTGACCTTCAGCTAAAACGCTGAGAATATGTCATTCTTCAAGAGAATGAAGCCGGAATGGTTACCGGGAATTTGTAAATCAATTATATCAGATGTAATATTACAATTCAATTAGAAAAGa                          >  1:1068440/1‑141 (MQ=255)
                                                                                                                      agGCCAGATGACGTATCATCTGACCTTCAGCTAAAACGCTGAGAATATGTCATTCTTCAAGAGAATGAAGCCGGAATGGTTACCGGGAATTTGTAAATCAATTATATCAGATGTAATATTACAATTCAATTAGAAAAGAAc                        >  2:736182/1‑141 (MQ=255)
                                                                                                                       ggCCAGATGACGTATTATCTGACCTTCAGCTAAAACGCTGAGAATATGTCATTCTTCAAGAGAATGAAGCCGGAATGGTTACCGGGAATTTGTAAATCAATTATATCAGATGTAATATTACAATTCAATTAGAAAAGAACt                       >  1:890773/1‑141 (MQ=255)
                                                                                                                                  gTATCATCTGACCTTCAGCAAAAACGCTGAGAATATGTCATTCTTCAAGAGAATGAAGCTGGAATGGTTACCGGGAATTTGTAAATCAATTATATCAGATGTAATATCACAATTCAATTAGAAAAGAACTTTATCTTTAAt            >  2:667688/1‑141 (MQ=255)
                                                                                                                                  acgtcatcTGGCCTTCAGCTAAAACGCTGAGAATATGTCATTCTTCAAGAGAATGAAGCCGGAATGGTTACCGGGAATTTGTAAATCAATTATATCAGATGTAATATTACAATTCAATTAGAAAAGAACTTTATCTTTAAt            >  2:452447/4‑141 (MQ=255)
                                                                                                                                  acgtcatcTGGCCTTCAGCTAAAACGCTGAGAATATGTCATTCTTCAAGAGAATGAAGCCGGAATGGTTACCGGGAATTTGTAAATCAATTATATCAGATGTAATATTACAATTCAATTAGAAAAGAACTTTATCTTTAAt            >  2:641799/4‑141 (MQ=255)
                                                                                                                                    atcatcTGACCTTCAGCAAAAACGCTGAGAATATGTCATTCTTCAAGAGAATGAAGCCGGAATGGTTACCGGGAATTTGTAAATCAATTATATCAGATGTAATATTACAATTAAATTAGAAAAGAACTTTATCTTTAATTg          >  2:371551/1‑141 (MQ=255)
                                                                                                                                          tGACCTTCAGCTAAAACGCTGAGAATATGTCATTCTTCAAGAGAATGAAGCCGGAATGGTTACCGGGAATTTGTAAATCAATTATATCCGATGTAATATTACAATTCAATTAGAAAAGAACTTTATCTTTAATTGCTTTTc    >  1:327150/1‑141 (MQ=255)
                                                                                                                                            aCCTTCAGCTAAAACGCTGAGAATATGTCATTCTTCAAGAGAATGAAGCCGGAATGGTTACCGGGCATTTGTAAATCAATTATATCAGATGTAATATTACAATTCAATTAGAAAAGAACTTTTTCTTTAATTGCTTTTCgt  >  2:136873/1‑140 (MQ=255)
                                                                                                                                            aCCTTCAGCTAAAACGCTGAGAATATGTCATTCTTCAAGAGAATGAAGCCGGAATGGTTACCGGGAATTTGTAAATCAATTATATCAGATGTAATATTACAATTCAATTAGAAAAGAACTTTATCTTTAATTGCTTTTCGa  >  2:648086/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                            
CGATAATAGTGACATGTGGCTTATAACACATTGTGTAATTTATTGTGTGTTTTGTGATGTCTAATATTGATTGCAGATTGTTGTATATTTCACTTAAAGGGCATTTATATAAAAAAAGAGGCCAGATGACGTATCATCTGACCTTCAGCTAAAACGCTGAGAATATGTCATTCTTCAAGAGAATGAAGCCGGAATGGTTACCGGGAATTTGTAAATCAATTATATCAGATGTAATATTACAATTCAATTAGAAAAGAACTTTATCTTTAATTGCTTTTCGA  >  CP000730/442082‑442362

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: