Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,105,994 T→C 69.2% T12T (ACT→ACC pdhD → dihydrolipoyl dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,105,9940TC69.2% 177.9 / 78.4 121T12T (ACT→ACCpdhDdihydrolipoyl dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (17/20);  new base C (45/36);  total (64/57)
Fisher's exact test for biased strand distribution p-value = 4.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 125 total aligned reads displayed.

AGCTTTGACCACAGACAAATTGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACTATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTCCTTCAAAAGCATTACT  >  CP000730/1105854‑1106134
                                                                                                                                            |                                                                                                                                            
aGCTTTGACCACAGACAAATTGATGGTGCACCTGGCCAAAATGAACTGCATCACATTAGCCGTTTATTAACTAAACCAGAATTATAATTCATGATGGGGTACAACATAGTAGTCGAAGATTTCCCAGTTGAAACAGGTACt                                                                                                                                              >  2:928242/1‑141 (MQ=255)
    ttGACCACAGACAAATTGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGGACCAGATACTATAg                                                                                                                                          >  2:677014/1‑141 (MQ=255)
             gACAAATTGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAg                                                                                                                                 <  2:1033305/141‑1 (MQ=255)
               cAAATTGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCa                                                                                                                               <  1:90686/141‑1 (MQ=255)
                    ttatcttGCAATTTGCCAAAATGCAATCAATCACATTAAACGTTGATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACTTGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTcc                                                                                                                          <  2:620192/135‑1 (MQ=255)
                    tGATGGTGCAAGTGGTCAATATGCTATGAATCACATTAAATGTTTATTAAATTATCAGGTTTTATTATTAATGGAGGGGTAAAACGTGGTAGTGGGAGATTTCCGAATTGAAACAGATACTATAGTGATCGGAGCAGGTcc                                                                                                                          <  2:128423/141‑1 (MQ=255)
                    tGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACAGGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTcc                                                                                                                          <  2:350812/141‑1 (MQ=255)
                       tGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATATCATAGTAATCGGAGCAGGTCCtgg                                                                                                                       >  1:214728/1‑141 (MQ=255)
                           ggaaCTGGCCAAAATGCAATGAATCACATTAAACGGTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACTATAGTAATCGGAGCAGGTCCTGGTGGa                                                                                                                   <  2:12672/139‑1 (MQ=255)
                           gCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGa                                                                                                                   <  1:53287/141‑1 (MQ=255)
                            cAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACTATAGTAATCGGAGCAGGTCCTGGTGGAt                                                                                                                  <  1:1031468/141‑1 (MQ=255)
                                  gCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAGAGCTGGTAGTTGGAGATTTCCCAATTGAAACAGATACTATAGCAATCCGAGCAGGTCCTGGTGGATAAGTTg                                                                                                            >  2:1024711/1‑141 (MQ=255)
                                  gCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTg                                                                                                            <  2:260660/141‑1 (MQ=255)
                                   ccAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTgc                                                                                                           >  1:136191/1‑141 (MQ=255)
                                   ccAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCAGGTGGATACGTTgc                                                                                                           >  1:94120/1‑141 (MQ=255)
                                     aaaaTGCAATGAATCACATGAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTGGGAGATTTCCCAATTGAAACAGATACTATAGTAATCGGAGCAGGTCCTGGTGGATACGTTgcag                                                                                                         <  2:1132719/141‑1 (MQ=255)
                                           cAACGAACCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTc                                                                                                   <  1:392138/141‑1 (MQ=255)
                                             aTGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGCGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGt                                                                                                 >  1:1126717/1‑141 (MQ=255)
                                              tGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACTATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTg                                                                                                >  2:826632/1‑141 (MQ=255)
                                              tGAATCACATTAAACGTTTATTAAATAACCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACTATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCCGCAATTCGTg                                                                                                >  2:287693/1‑141 (MQ=255)
                                              tGAATCACATTAAACGGTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACTATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTg                                                                                                <  1:957336/141‑1 (MQ=255)
                                              tGAACCACTCTAAACGTTTATTAATTCATCTAGAATTATTATTAATGGAGATGTAAAATATGGTAGTTGGAGATTTCCCAATTGAAACATATACCATAGTAATCGGATCAGTTCCTGTTGTTTACGTTGCAGCAATTCTTg                                                                                                <  2:939385/141‑1 (MQ=255)
                                               gAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACTATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGc                                                                                               <  2:1007559/141‑1 (MQ=255)
                                               gAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGc                                                                                               <  1:258162/141‑1 (MQ=255)
                                                aaTCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCa                                                                                              <  1:257356/141‑1 (MQ=255)
                                                 aTCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACTATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAg                                                                                             <  1:223608/141‑1 (MQ=255)
                                                  tCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACTATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGc                                                                                            <  2:771414/141‑1 (MQ=255)
                                                    acaTTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACTATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTc                                                                                          >  1:873833/1‑141 (MQ=255)
                                                     caTTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCa                                                                                         <  1:472913/141‑1 (MQ=255)
                                                      aTTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGTAGCTCaa                                                                                        >  2:25302/1‑141 (MQ=255)
                                                      aTTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCaa                                                                                        >  2:595800/1‑141 (MQ=255)
                                                        tAAACGTTTATTAAATAATCCAGCATTATTATTAATGGAGGGGTGAAACATGGTAGTTGGAGATTTCGCAATTGAAACGGATACCATAGTAATCGGAGCAGGTCCTGGTGTATACGTTGCCGCAATTCGTGCAGCTCaagt                                                                                      >  1:163435/1‑139 (MQ=255)
                                                             gTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGAc                                                                                 <  1:559680/141‑1 (MQ=255)
                                                               ttattaAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACTATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACaa                                                                               >  2:171011/1‑141 (MQ=255)
                                                               ttattaAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACaa                                                                               >  1:541370/1‑141 (MQ=255)
                                                                    aaaTAATCCAGCATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCACTTCGTGCAGCTCAATTAGGACAAAAAGt                                                                          <  1:372826/141‑1 (MQ=255)
                                                                    aaaTAATCCAGAATTCTTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGt                                                                          >  2:299104/1‑141 (MQ=255)
                                                                    aaaTAATCCAGAATTATTAGTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGt                                                                          <  1:15579/141‑1 (MQ=255)
                                                                     aataatCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACTATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTa                                                                         <  2:67447/141‑1 (MQ=255)
                                                                       taatCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATAGGTTGCAGCAATTCGTGCAGCTTAGTTAGGACAAAAAGTagc                                                                       >  1:443089/1‑139 (MQ=255)
                                                                           ccAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACaagc                                                                   >  2:614669/1‑139 (MQ=255)
                                                                           ccAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATc                                                                   >  2:326114/1‑141 (MQ=255)
                                                                           ccAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATc                                                                   >  2:704899/1‑141 (MQ=255)
                                                                           ccAGAATTATTATTAACGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATc                                                                   >  1:713437/1‑141 (MQ=255)
                                                                             aGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGt                                                                 >  1:615077/1‑141 (MQ=255)
                                                                                  tattattaATGGAGGGGTAAAACATGGTCGGTGGAGATTTCCCAATTGATCCAGACAAAATAAAAAAGGGAGCATTGCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTgaga                                                            >  2:298370/1‑141 (MQ=255)
                                                                                  tattattaATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTgaga                                                            >  1:742485/1‑141 (MQ=255)
                                                                                   attattaATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTgagga                                                           >  1:1014491/1‑139 (MQ=255)
                                                                                    ttattaATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGaaa                                                          >  2:385125/1‑141 (MQ=255)
                                                                                      attaATGGAGGGGTAAAACTTGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACCAAAAGTAACAATCGTTGAGAAAgg                                                        <  1:764190/141‑1 (MQ=255)
                                                                                          aTGGAGGGGTAAAACATTGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAAt                                                    <  1:714923/141‑1 (MQ=255)
                                                                                           tGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATc                                                   >  2:649808/1‑141 (MQ=255)
                                                                                               ggggTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACTATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTtgg                                               >  2:1045855/1‑141 (MQ=255)
                                                                                               ggggTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACTATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTtgg                                               >  1:293249/1‑141 (MQ=255)
                                                                                                gggTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATATtggt                                              >  1:609734/1‑141 (MQ=255)
                                                                                                ggatAAAACATTGTAGTGGGAGGTTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGCTCCTGGTGGATACGTTGCAGCAATTCGTGCAGAAAAAAAAAAAAAAAAAGTAACAATCGTTGAGAAAGGTAATCTtggt                                              <  2:713437/138‑1 (MQ=255)
                                                                                                 ggTACAACATGGTTGTTGGAGGGTTGCCAATGGAAACAGAGACTATAGTAATCGGAGCAGGTCCGGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTGGGGCAGAAAGTAACAATCGTTGAGAAAGGTAATCTtggtg                                             <  2:731806/141‑1 (MQ=255)
                                                                                                  gTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACTATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTtggtgg                                            <  1:530977/141‑1 (MQ=255)
                                                                                                   tAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCCGGTCCTGGTGGATACGTTGCAGGCATTCGTGCATCTCGAGTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTggtggt                                           >  2:1149839/1‑141 (MQ=255)
                                                                                                   tAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTggtggt                                           >  1:155846/1‑141 (MQ=255)
                                                                                                    aaaaCATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGgtggtg                                          >  1:856794/1‑141 (MQ=255)
                                                                                                     aaaCATGGTAGTTGGAGATTTCCCAATTGAAACAGATACTATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCACAAGTAGGACGGATGGTAATAATCGTTGAGAAAGGTAATCTTGGTGgtgt                                         >  1:388391/1‑141 (MQ=255)
                                                                                                     aaaCATGGTAGTTGGAGATTTCCCAATTGAAACAGATACTATAGTAATCGGAGAAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTTACAATCGTTGAGAAAGGTAATCTTGGTGgtgt                                         >  1:802043/1‑141 (MQ=255)
                                                                                                     aaaCATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGgtgt                                         >  2:50375/1‑141 (MQ=255)
                                                                                                     aaaCATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGgtgt                                         <  1:984568/141‑1 (MQ=255)
                                                                                                     aaaCATGGTAGTTGGAGATTACCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGAAAAAGTAGGACAAAAAGTAACAATCGTTGAGAAAGAAAATCTTGTTGgtgt                                         >  1:749973/1‑141 (MQ=255)
                                                                                                      aaCATGTGAGTTGGAGATTACCCAAGTGAAACAGATACCATAGTAATCCGAGCAGGTCCTGGTGGATACGTTGCAGCAATTTGTGCAGCTAAAAAAAAAAAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGtt                                        <  2:1063971/141‑1 (MQ=255)
                                                                                                      aaCATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGGCAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGtt                                        >  2:1008877/1‑141 (MQ=255)
                                                                                                        cATGGTAGTTGGAGATTTCCCAATTGAAACAGATACTATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTg                                      <  1:671952/141‑1 (MQ=255)
                                                                                                         aTGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGc                                     <  1:975130/141‑1 (MQ=255)
                                                                                                          tGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCt                                    <  1:1156541/141‑1 (MQ=255)
                                                                                                           ggcaGTTGGATATTTCCCAAGTGAAACATATACCATAGTAATCGGAGCAGGTACTGGTGGATACGTTGCAGCCATTCGTGCAGATCAATTAAGACAAAAAGTAACAATCGTTCAGAACGGTAATCTCGGTGGTGTTTGCtt                                   >  2:436649/4‑141 (MQ=255)
                                                                                                           ggTAGTTGGAGATTTCCCAATTGAAACAGATACTATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTGGAGAAAGGTAATCTTGGTGGTGTTTGCtt                                   >  1:362049/1‑141 (MQ=255)
                                                                                                           ggTAGGTGGAGATTTCCCAATTGAAACAGATACTATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCtt                                   <  2:1194834/141‑1 (MQ=255)
                                                                                                              aGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATAGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGACAGGTACTCTTGGTGGTGTTTGCTTaaa                                >  2:297189/1‑141 (MQ=255)
                                                                                                                ttGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGGGAAAGGTAATCTTGGTGGTGTTTGCTTAAACg                              >  1:1102095/1‑141 (MQ=255)
                                                                                                                ttGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACg                              >  1:844898/1‑141 (MQ=255)
                                                                                                                taatagaTTTCGCAATTGAAAGAGATACTATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGTGAATCTTGGTGGTGTTTGCTTAAACg                              <  2:1155110/137‑1 (MQ=255)
                                                                                                                   gagaTTTCCCAATTGAAACAGATACTATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAg                           >  1:957961/1‑141 (MQ=255)
                                                                                                                   gagaTTTCCCAATTGAAACAGATAATATAGTAATCGGAGCAGGTCATAATCGCTCCTTTTCAGAAATACGTGCTCCTCAATTAGGACAAGAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAg                           >  1:875754/1‑141 (MQ=255)
                                                                                                                    agaTTTCCCAATTGAAACAGATACTATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAgg                          >  1:312264/1‑141 (MQ=255)
                                                                                                                     gaTTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGa                         <  1:1085246/141‑1 (MQ=255)
                                                                                                                      aTTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGAt                        >  1:127925/1‑141 (MQ=255)
                                                                                                                      aTTTCCCAAATGAAAAAGAACATATGGTAATCGGAGCAGGTCCTGGTGGATACGGTGAAGCAATTTGTGCAGTAAAAAAAAAAAAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGAt                        <  2:499431/141‑1 (MQ=255)
                                                                                                                       tgtCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACACTCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTTGGATg                       >  2:541809/3‑141 (MQ=255)
                                                                                                                       cttCCCAATTGAAACAGAAACGAGAGTACTCGTAGCGGGACATGGTGGATACGTGGCAGCAATTCGTCCAGCTAACATCGGGAAATCATCGACCGACGGTGAGCCAGGTAATCTTGGTGGTGTTTGCATAAACGTAGGATg                       <  2:544261/140‑1 (MQ=255)
                                                                                                                        ttCCCAATTGAAACAGATACTATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAACCGTTGAGAAAGTTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGt                      <  1:171011/141‑1 (MQ=255)
                                                                                                                           ccAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTAtt                   <  1:1018879/141‑1 (MQ=255)
                                                                                                                           ccAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTAtt                   <  1:698918/141‑1 (MQ=255)
                                                                                                                            cAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTc                  >  2:733234/1‑141 (MQ=255)
                                                                                                                               ttGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGCTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTCCtt               >  2:215574/1‑141 (MQ=255)
                                                                                                                                 gAAACATATACTAGTGTACTCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTAAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTCCTTCa             <  2:882713/141‑1 (MQ=255)
                                                                                                                                 gAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTCCTTCa             >  2:685066/1‑141 (MQ=255)
                                                                                                                                   aaCAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTCGAAAAAAATCTTGCACCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTCCTTCaaa           >  2:430556/1‑141 (MQ=255)
                                                                                                                                     aaGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCCATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTCCTTCAAAAg         <  1:959195/140‑1 (MQ=255)
                                                                                                                                         tACTATAGTAATGCGATCAGGCCTGGGTGCATACGTTGTAGTAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAACCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTCCTTCAAAAGCAtt     <  2:1115615/141‑1 (MQ=255)
                                                                                                                                         tACTATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTCCTTCAAAAGCAtt     <  1:921303/141‑1 (MQ=255)
                                                                                                                                         tACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTCCTTCAAAAGCAtt     >  1:1183754/1‑141 (MQ=255)
                                                                                                                                         tACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTCCTTCAAAAGCAtt     <  1:955121/141‑1 (MQ=255)
                                                                                                                                            tataGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTCCTTCAAAAGCATtact  >  1:911200/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                            
AGCTTTGACCACAGACAAATTGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACTATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTCCTTCAAAAGCATTACT  >  CP000730/1105854‑1106134

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: