Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,963,768 C→G 23.5% V365L (GTA→CTA)  hemG ← protoporphyrinogen oxidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,963,7680CG23.5% 282.1 / 90.4 140V365L (GTA→CTA) hemGprotoporphyrinogen oxidase
Reads supporting (aligned to +/- strand):  ref base C (59/48);  new base G (18/15);  total (77/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.05e-01

Only 100 of 142 total aligned reads displayed.

CATACTTTTCGGCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTATTTTCAATGTCTTTT  >  CP000730/1963628‑1963907
                                                                                                                                            |                                                                                                                                           
cATACTTTTCGGCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAc                                                                                                                                             <  1:970016/141‑1 (MQ=255)
 aTACTTTTCGGCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCACTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACa                                                                                                                                            <  1:823700/141‑1 (MQ=255)
   aCTTTTCGGCAAACGAATGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATa                                                                                                                                          >  1:712900/1‑141 (MQ=255)
    cTTTTCGGCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATAc                                                                                                                                         <  1:1063157/141‑1 (MQ=255)
      tttCGGCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGc                                                                                                                                       >  2:1136823/1‑141 (MQ=255)
      tttCGGCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGc                                                                                                                                       >  2:143284/1‑141 (MQ=255)
      tttCGGCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACATGGCTTACCTACATCCGc                                                                                                                                       >  2:52043/1‑141 (MQ=255)
        tCGTCAAACCATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCAc                                                                                                                                     <  1:305513/141‑1 (MQ=255)
        tCGGCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACGACAGTATCACCTGGTTTACCTACATACGCAc                                                                                                                                     >  2:640393/1‑141 (MQ=255)
        tCGGCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGCTTACCTAGATACCCAc                                                                                                                                     >  1:596038/1‑141 (MQ=255)
        tCGGCAAACGAGTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCCCAGAATCACCTGGTTTACCTACATACGCAc                                                                                                                                     >  2:937911/1‑141 (MQ=255)
         cGGCAAACGATTGACACTTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACg                                                                                                                                    <  2:1016295/141‑1 (MQ=255)
         cGGCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATActctcg                                                                                                                                    <  2:1037036/141‑6 (MQ=255)
         cGGCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACg                                                                                                                                    <  1:183022/141‑1 (MQ=255)
         cGGCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACg                                                                                                                                    <  2:1118846/141‑1 (MQ=255)
          ggCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGa                                                                                                                                   <  2:895934/141‑1 (MQ=255)
          ggCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTAAAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGa                                                                                                                                   <  2:576020/141‑1 (MQ=255)
             aaaCGATTGACAATGGTAAATTCGGGATCACCTTTAAATGTCATCATTTGACTTAAATCGCTACGTACAATCGATACTAATTCATTATCTGTATGATCATGAGCCACAGTATCACCTGGTTTACCTACCTACCACCGAATc                                                                                                                                >  2:640848/1‑141 (MQ=255)
              aaCGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCa                                                                                                                               >  1:1139370/1‑141 (MQ=255)
               aCGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCCGAAATCaa                                                                                                                              >  2:99301/1‑141 (MQ=255)
               aCGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCaa                                                                                                                              <  1:527730/141‑1 (MQ=255)
                 gATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCaaaa                                                                                                                            >  1:851531/1‑141 (MQ=255)
                  aTTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAac                                                                                                                           >  1:305366/1‑141 (MQ=255)
                  aTTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAac                                                                                                                           >  1:302014/1‑141 (MQ=255)
                  aTTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGACCGAATCAAAac                                                                                                                           >  2:241371/1‑141 (MQ=255)
                  aTTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGCACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAac                                                                                                                           >  2:1110920/1‑141 (MQ=255)
                   ttGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACACCAGTATCACCTGGTTTAACTACATACGCCCGAATCAAAacc                                                                                                                          >  2:472516/1‑141 (MQ=255)
                    tGACAATTGTAAATTCAGGATCACTTTTAAATGTCATCATTTGACTTAAAGCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAacct                                                                                                                         <  2:522160/141‑1 (MQ=255)
                         aTTGTAAATTCAGGATCACCTTGAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCAGAGTAGCACCTGGTTTACCTAGATACGCACGAATCAAAaccttacct                                                                                                                    <  1:525365/141‑1 (MQ=255)
                              aaaTTCAGGATCCCCTTTAAATGGCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATGCATTATCTGTATGACCATCAACCACAGTATCCCCCGGTTTACCTACATCCCCCCGAATCAAAACCTTACCCTcccg                                                                                                               >  2:347451/1‑139 (MQ=255)
                              aaaTTCAGGATCACCTTTAAATGTCAGCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCgg                                                                                                               <  2:171079/141‑1 (MQ=255)
                               aaTTCAGTATTACGTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGgt                                                                                                              <  2:946842/141‑1 (MQ=255)
                                aTTCAGGATCACCTTTAACTGTTATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGgtg                                                                                                             <  1:468358/141‑1 (MQ=255)
                                aTTCAGGATCACCTTAAAATGTCATCATTTGACTTAAATCTCTACGCACAATCGATACTACAACCTTATCTGTATGACCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAACCCTTACCTTCCGgtg                                                                                                             <  1:457711/141‑1 (MQ=255)
                                 ttCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGgtgt                                                                                                            <  2:467867/141‑1 (MQ=255)
                                 ttCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGgtgt                                                                                                            <  2:483468/141‑1 (MQ=255)
                                     ggATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAACCGATAATACTTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGCTgtagta                                                                                                        >  1:166196/1‑141 (MQ=255)
                                      gATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTaa                                                                                                       <  1:425101/141‑1 (MQ=255)
                                        tCACCTGTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAAt                                                                                                     >  2:1066070/1‑141 (MQ=255)
                                          agcTTTAAATGTGATCGTTTGACTTAAATCTTTACGGACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATgg                                                                                                   <  2:861457/139‑1 (MQ=255)
                                           ccTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGc                                                                                                  <  1:259960/141‑1 (MQ=255)
                                           ccTTTAAATGTCATCATTTGAATTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGc                                                                                                  <  1:27687/141‑1 (MQ=255)
                                              ttAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGccct                                                                                               >  2:52392/1‑139 (MQ=255)
                                                   tGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAAACACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTCGTAAATGGCCAtttttt                                                                                          >  2:303442/1‑141 (MQ=255)
                                                      catcatTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGa                                                                                       >  1:1163041/1‑141 (MQ=255)
                                                      catcatTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGa                                                                                       >  1:897530/1‑141 (MQ=255)
                                                       ggcatTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTAACTACATACGCACTAATCAAAACCTTCCATTCCGGATTAGTAAATGGCCATTTTTTCGAt                                                                                      >  2:190896/3‑141 (MQ=255)
                                                       atcatTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGAt                                                                                      >  1:499962/1‑141 (MQ=255)
                                                        tcatTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGAtt                                                                                     >  2:413723/1‑140 (MQ=255)
                                                           tttGACTTAAATCTCTACGTACAACCGGTACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTcc                                                                                  <  2:71879/141‑1 (MQ=255)
                                                              gACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAg                                                                               <  1:948307/141‑1 (MQ=255)
                                                               aCTTAAATCTCTACGTACAATCGATTCTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGt                                                                              <  1:153258/141‑1 (MQ=255)
                                                               aCTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAACCCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGt                                                                              <  2:986364/141‑1 (MQ=255)
                                                               aCTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGt                                                                              <  1:143284/141‑1 (MQ=255)
                                                                gttAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGtc                                                                             >  2:735502/2‑140 (MQ=255)
                                                                cTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTCCCGGTGTTGTAAATGGCCATTTTTTCGTTGTCCAAGtc                                                                             >  2:1001827/1‑140 (MQ=255)
                                                                 ttAAATCTCTACGTACACTCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTAc                                                                            <  1:153898/141‑1 (MQ=255)
                                                                   aaaTCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGACTTCCGAGTAAAt                                                                          >  2:997075/1‑141 (MQ=255)
                                                                    aatccgtACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATg                                                                         <  2:156413/135‑1 (MQ=255)
                                                                      tctcTACGTACAATCTATACTAACTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCg                                                                       <  1:411515/141‑1 (MQ=255)
                                                                        tctACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACGTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACAGTCGGt                                                                     >  1:36154/1‑141 (MQ=255)
                                                                        tctACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCAACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGt                                                                     <  2:891696/141‑1 (MQ=255)
                                                                           aCGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAAt                                                                  >  1:1037095/1‑141 (MQ=255)
                                                                               aCAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTTCAAGTGCATGCGGTaaggact                                                              >  1:1169669/1‑136 (MQ=255)
                                                                                cAATCGATACTAATTCATTATCTGTATGATCATCCACCACAGTGTCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAGTGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTg                                                             <  2:245780/141‑1 (MQ=255)
                                                                                  tttgATACCAATGCTGTATCTGCTTGTTCATTATTCACTTTATCACTTGGTTTTCCTACTTACGCACTAATCAAAACCTTACCTTCCGGTGTAGTATATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTa                                                           <  2:523863/138‑1 (MQ=255)
                                                                                    cGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATc                                                         >  2:271742/1‑141 (MQ=255)
                                                                                       tACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACt                                                      >  1:256693/1‑141 (MQ=255)
                                                                                        aCTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTcgtgttcactc                                                     >  2:230257/1‑131 (MQ=255)
                                                                                            tttttttttttGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATAGGCACGTATCAAAACCTTACCTTCCGTTTTTGTATTTTGCTATTTTTTCGATGTCCCAGTCCATTCGGTATTGTCTGTTTTACTCGttt                                                 >  2:795811/11‑140 (MQ=255)
                                                                                            aTTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTCCCTACATACGCACGAATCAAAACCTTACCTTCCGGCGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTACCACTCGTtc                                                 >  2:1170876/1‑141 (MQ=255)
                                                                                                aTTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTTTTTTTCTTAGTACATGCGGTAATGTCCGTATCACTCGTTCTCGc                                             >  2:138355/1‑141 (MQ=255)
                                                                                                     cTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTa                                        >  1:641077/1‑141 (MQ=255)
                                                                                                      tGTATGATCATCAACCCCAATGTCACCTGGTTTACCTACATACGCACGAATGAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCCATTTCCCAGGACATGCGGTAATGGCGGTATCACTCGTTCTCGcgactag                                       >  2:850595/1‑135 (MQ=255)
                                                                                                          tGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCGCGAATAAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAAGGTCTATGTCACTCGTTCTCGCACTTACGAAg                                   >  1:1127592/1‑141 (MQ=255)
                                                                                                          tGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAg                                   >  1:1127896/1‑141 (MQ=255)
                                                                                                          tGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCACAATTACGAAg                                   >  1:820781/1‑141 (MQ=255)
                                                                                                          tGATCATCAACCAACGTATCACCTGGTTTACCTACATACGCACAAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCGATTACGAAg                                   >  1:235838/1‑141 (MQ=255)
                                                                                                           gATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTGCTTTCTTTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGc                                  >  1:513658/1‑141 (MQ=255)
                                                                                                           gATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGc                                  <  2:1139370/141‑1 (MQ=255)
                                                                                                            atcatcAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGAGGTCCAAGTACATGCGGTAATGTCGGTATCACTCGTTCTCGCAATCACGAAGcc                                 >  2:460768/1‑141 (MQ=255)
                                                                                                             tcatcaACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGcaa                                >  1:925960/1‑139 (MQ=255)
                                                                                                              catcaACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAg                               <  1:478324/141‑1 (MQ=255)
                                                                                                                tcaACCACCGTCTCACCTGGTTTACCGTCTTACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTa                             <  1:610405/141‑1 (MQ=255)
                                                                                                                   aCCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATTGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTAGCAATTACGTAGCCAGTAccc                          >  1:30212/1‑140 (MQ=255)
                                                                                                                    ccACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCCGTGCcaa                         >  2:41881/1‑140 (MQ=255)
                                                                                                                    ccACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGTGGTAGTAAATGGCCATGTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTAACGCAATTCCGAAGCCAGTACcat                         >  2:185442/1‑141 (MQ=255)
                                                                                                                      acaGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTCGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCCCGCAATTACGAAGCCAGTACcatca                       >  2:449359/1‑141 (MQ=255)
                                                                                                                       caGTATCACCTGGTTTACCTGGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACcatcat                      <  2:168432/141‑1 (MQ=255)
                                                                                                                       caGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCCGTATCACTCTTTCTCGCAATTACGAAGCCAGTACcatcat                      <  1:1066070/141‑1 (MQ=255)
                                                                                                                           ttCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGt                  <  2:941867/140‑1 (MQ=255)
                                                                                                                           aTCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCCAGTACATGAGGTAATGTCTGTATCACTCTTTCTCGCAATTACGAAGCCAGTAAcatcagcggt                  >  2:375555/1‑136 (MQ=255)
                                                                                                                               ccTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTAttt              >  1:1199567/1‑141 (MQ=255)
                                                                                                                                cTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGACCATTTGTTCGATGTCCAAGTACGTGCGGTAATGTCTGTATCACTCGTTCTCGCAATTCAGAAGCCAGTACCATCATGGGCAgttt             >  2:328331/1‑141 (MQ=255)
                                                                                                                                 tGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTATTTTc            <  2:36154/141‑1 (MQ=255)
                                                                                                                                  cgcttACCTACATACACACCAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGACGTCCAAGTACATGCGGTAATGTCCGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTATTTTCa           <  2:268729/138‑1 (MQ=255)
                                                                                                                                     ttACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTCTCACTCGTTCGCGCAATTACGAAGCCAGTACCATCATGGGTCGTTTCaagg        >  1:682544/1‑139 (MQ=255)
                                                                                                                                        ccTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTATTTTCAATGTCt     >  1:445929/1‑141 (MQ=255)
                                                                                                                                         cTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTATTTTCAATGTCtt    <  1:397507/141‑1 (MQ=255)
                                                                                                                                           aCATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGTTGTCGAAGTGCATGCGCGAATGTCTGTATCACTGGTTCTCGCAATTACGCAGCCAGTACCATCATGGGTATTTTCGCTGTCtttt  >  2:960416/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                           
CATACTTTTCGGCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTATTTTCAATGTCTTTT  >  CP000730/1963628‑1963907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: