Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	2,028,583	G→T	24.9%	A21D (GCC→GAC)	yvqF ←	hypothetical membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,028,583	0	G	T	24.9%	134.5 / 33.8	73	A21D (GCC→GAC)	yvqF	hypothetical membrane protein
Reads supporting (aligned to +/- strand):  ref base G (27/27);  new base T (7/11);  total (35/38)
Fisher's exact test for biased strand distribution p-value = 5.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.62e-01

AATTAATAGCGCGCCTATCCAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGTTTTTTCTGTTGCTATTTTTTCAGTTG  >  CP000730/2028444‑2028723                                                                                                                                            |                                                                                                                                             aaTTAATATCGCGCCCATCCAAAACGCCAAAAGGCCACGTATGTCATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGc                                                                                                                                             <  1:618521/141‑1 (MQ=255) aaTTAATAGCGCGCCTATCCAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGc                                                                                                                                             <  1:791307/141‑1 (MQ=255)  aTTAATAGCGCGCCTATCCAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCa                                                                                                                                            <  2:36602/141‑1 (MQ=255)   ttAATAGCGCGCCTATCCAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCaa                                                                                                                                           <  1:1000738/141‑1 (MQ=255)     aaTCGCGCGCCTATCAAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGCGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCaata                                                                                                                                         <  1:500496/141‑1 (MQ=255)     aaTAGCGCGCCTATCCAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCaata                                                                                                                                         >  1:781495/1‑141 (MQ=255)      aTAGCGCGCCATTCCAAAACGCCAAAAGGCCACGCATTTCATGAAAATAAAGATAACCTACATAAACTAGTACACTTCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCaataa                                                                                                                                        <  2:342340/141‑1 (MQ=255)       tAGCGCGCCTATCCAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCaataat                                                                                                                                       >  2:498686/1‑141 (MQ=255)       tAGCGCGCCTATCCAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAAAATGTAGTAAAAATTGGCaataaa                                                                                                                                       >  2:703799/1‑140 (MQ=255)        aGCGCGCCTATCCAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGTCaataata                                                                                                                                      >  1:106861/1‑140 (MQ=255)        aGCGCGCCTATCCAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATc                                                                                                                                      <  1:434513/141‑1 (MQ=255)             gcCTATCCAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTaa                                                                                                                                 <  2:611203/141‑1 (MQ=255)                 aTCCAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATCGAACGAGTAAAAAGACAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCa                                                                                                                             <  1:792559/141‑1 (MQ=255)                   ccAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGTCAATAATCATTAATGCAGt                                                                                                                           <  1:1157123/141‑1 (MQ=255)                      aaaCGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAACATATGTAGTAAAAATGGTCAATAATCATTAGTGCAGTaaa                                                                                                                        >  2:1155974/1‑141 (MQ=255)                      aaaCGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTaaa                                                                                                                        >  1:18688/1‑141 (MQ=255)                           ccAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGa                                                                                                                   >  2:830321/1‑141 (MQ=255)                              aaaGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCa                                                                                                                >  1:500553/1‑141 (MQ=255)                                aGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAAc                                                                                                              <  2:1070496/141‑1 (MQ=255)                                 ggCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGCGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACa                                                                                                             >  2:15078/1‑141 (MQ=255)                                 ggCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACa                                                                                                             <  2:941571/141‑1 (MQ=255)                                 ggCCAACTATTTTAAGAAAATAAAGATATCCTACATAAACTAATACACATCCAAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAACATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACa                                                                                                             <  2:301634/141‑1 (MQ=255)                                  ggcACGTAATTAATAAAACTAAATATATCCTACAATAACTCATACACTGTCCAATAATAGACCGAGGAAAAAGCAAAATTTTTCAAAAAATATGGAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAActt                                                                                                            <  2:514572/139‑3 (MQ=255)                                     aCGTATTTTATTAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGTCAATAATCATTAATGCAGTAAAAATGATCAACATTTg                                                                                                         <  2:1163018/141‑1 (MQ=255)                                     aCGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGTCAATAATCATTAATGCAGTAAAAATGATCAACATTTg                                                                                                         >  2:687575/1‑141 (MQ=255)                                     aCGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACAtt                                                                                                           >  2:765765/1‑139 (MQ=255)                                     aCGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACAtt                                                                                                           <  1:765765/139‑1 (MQ=255)                                        tATTTTATGAAAATAAAGATATCCTACATAAACTAATACAAATCCCAATAATAGAACGAGTAAAAAGCAAATTTTTTCAAAAAATATGTAGTAAAAATAAGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGt                                                                                                      >  2:563022/1‑141 (MQ=255)                                         aTTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGtt                                                                                                     >  2:459082/1‑141 (MQ=255)                                                 aaaaTAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGatatata                                                                                             >  1:563975/1‑141 (MQ=255)                                                         gATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGTCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTtgtgtg                                                                                     <  1:834729/141‑1 (MQ=255)                                                         gATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTtgtgtg                                                                                     <  2:172476/141‑1 (MQ=255)                                                           tatCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTc                                                                                   >  1:396381/1‑141 (MQ=255)                                                           tatCCTACAGAGACTAAGACACATCCCAATGATTGGACGAGTAAAAAGGCAATTTTGTCAAAAAATATGTAGTAAAAATTGGCAATGGGCATTAATGCAGTAAAAATGATCGACAGGTGCGTTGATATATATTTGTGTGTc                                                                                   <  2:157/141‑1 (MQ=255)                                                             cccaacaaacactactACACTTCACAAAAAAAGAACGAGTAAAAAGCCCTTTTTTTCAAAAAAAAAAAAAAAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCAt                                                                                 <  2:1145944/131‑1 (MQ=255)                                                              ccTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCCAAAAATATGTAGTAAAAATCGCCAATAATCATTAATGCAGTAAAAATGATCAACATTGGCGTTGATATATCTTTTTATGTCATg                                                                                >  2:740698/1‑141 (MQ=255)                                                                 aCATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGttt                                                                             >  1:198434/1‑141 (MQ=255)                                                                  cATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTc                                                                            <  1:849108/141‑1 (MQ=255)                                                                  cATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTc                                                                            <  2:841132/141‑1 (MQ=255)                                                                  cATAAACTAATACACATCACAATAATAGAACGAGTAAAAAGCCAATATAATAAAAAAATATGTAGTAAAAATTGTCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTc                                                                            <  2:640480/141‑1 (MQ=255)                                                                      aaCTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTcgccg                                                                        >  1:630049/1‑141 (MQ=255)                                                                           aTACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCttt                                                                   >  1:303904/1‑141 (MQ=255)                                                                               acaTCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGTCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTAt                                                               >  1:1038711/1‑141 (MQ=255)                                                                               acaTCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTAt                                                               >  1:988558/1‑141 (MQ=255)                                                                                caTCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTAGCCGCCTTTCTATg                                                              >  1:278584/1‑141 (MQ=255)                                                                                 aTCCCAATAATAGAACGAGTAAAAAGCAAAAATATTCAAAAAATATGTAGTAAAAATTGTCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGc                                                             <  2:247913/141‑1 (MQ=255)                                                                                  tCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGTCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGGGTCATGTTTGGCCGCCTTTCTTTGcc                                                            >  2:983717/1‑141 (MQ=255)                                                                                  cccaaaaaataGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTTGCCGCCTTTCTATGcc                                                            <  1:593503/134‑1 (MQ=255)                                                                                    ccAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGTCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCcac                                                          <  1:215268/141‑1 (MQ=255)                                                                                     cAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAACTATGTCGTTTTAATTTGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCcaca                                                         >  1:934514/1‑141 (MQ=255)                                                                                      aataatAGACCGATGAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAg                                                        <  2:88331/141‑1 (MQ=255)                                                                                      aataatAGAAGGAGTAAAAAGCAAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAg                                                        <  2:966570/141‑1 (MQ=255)                                                                                      aataatAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATACGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAg                                                        <  2:598600/141‑1 (MQ=255)                                                                                       ataataGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGTCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCTCCTTTCTATGCCACAGc                                                       >  2:268063/1‑141 (MQ=255)                                                                                          ataGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGtt                                                    >  2:276414/1‑141 (MQ=255)                                                                                            aGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGAAAAAATGATCAACATTTTCTTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCa                                                  <  2:513007/141‑1 (MQ=255)                                                                                             gAACGAGAAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCat                                                 <  1:678099/141‑1 (MQ=255)                                                                                              aaCGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGTCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCata                                                <  2:1125954/141‑1 (MQ=255)                                                                                               aCGAGTAAAAAGCCAATTTTTTCAAAAAAGATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCatat                                               <  2:30513/141‑1 (MQ=255)                                                                                                   gTAAAAAGCCAATTGTTGCAAAAAATGTGGAGGGGAAAGTGTCAATAATCAGTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAAtt                                           >  2:1187628/1‑141 (MQ=255)                                                                                                    tAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGTCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAAttt                                          <  1:687575/141‑1 (MQ=255)                                                                                                    tAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGTCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAAttt                                          <  1:1136909/141‑1 (MQ=255)                                                                                                     aaaaaGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTACTGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAATtta                                         >  1:692953/1‑141 (MQ=255)                                                                                                     aaaaaGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTGAGGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAATtta                                         >  1:692970/1‑141 (MQ=255)                                                                                                          gCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGCGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGa                                    >  2:818356/1‑141 (MQ=255)                                                                                                           ccAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGAt                                   >  1:467114/1‑141 (MQ=255)                                                                                                                ttttgCAAAAAATATGTAGTAAAAATTGTCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGAt                              <  2:242495/141‑1 (MQ=255)                                                                                                                 ttttCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATg                             >  2:1011528/1‑141 (MQ=255)                                                                                                                     cAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGtttt                         <  1:537991/141‑1 (MQ=255)                                                                                                                      aaaaaaTATGTAGTAAAAATTGTCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGttttt                        <  2:218368/141‑1 (MQ=255)                                                                                                                              tGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGTTTTTTCTGTTGc                >  1:851358/1‑141 (MQ=255)                                                                                                                               gtagtaAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCGCAGCGTTCATATAATTTCTTAGATACGATGTTTTTTCTGTTGCt               >  1:563556/1‑141 (MQ=255)                                                                                                                                       aaTTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGTTTTTTCTGTTGCTATTTTTTc       >  1:659700/1‑141 (MQ=255)                                                                                                                                           ggCAATAATAATTAATGCAGTAAAAATGATCAACATATGCGTTGATAAATATTTGTGTGTCATGTTTAGCCGCCTTTCTATGCCACAGCGTTAATATACTTTATTAGATACGATGTTTTATCTGTTGCTATTTTTTCAGtt   >  2:222718/1‑141 (MQ=255)                                                                                                                                            gCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGTTTTTTCTGTTGCTATTTTTTCAGTTg  <  2:753196/141‑1 (MQ=255)                                                                                                                                            |                                                                                                                                             AATTAATAGCGCGCCTATCCAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGTTTTTTCTGTTGCTATTTTTTCAGTTG  >  CP000730/2028444‑2028723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑