Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,123,847 T→A 7.7% intergenic (‑107/‑26) USA300HOU_2003 ← / → USA300HOU_2004 possible transcriptional regulator/possible bifunctional S24 family peptidase/transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,123,8470TA 7.7% 301.1 / 4.2 113intergenic (‑107/‑26)USA300HOU_2003/USA300HOU_2004possible transcriptional regulator/possible bifunctional S24 family peptidase/transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (54/49);  new base A (2/8);  total (56/57)
Fisher's exact test for biased strand distribution p-value = 9.38e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 123 total aligned reads displayed.

TAATCAGTCCTCAACTCTTTTAATGTTTTCATGTTGTCACCGCCTTTCGTAAACCTAATATAATACGAAATTTTCGTATTGTCAACATTAAATACGTTTTTTTCGTAAAAAACTTTACTATGATATGAAAATTTCGTATAATAAGAAAAAAGGAGGTAAGTAATATGAACAAAGAAAGAAATATTATTATAGCCAAAAACATTAGAAAATTTCTCAACGATTCAAATATGTCTCAAAAGAAACTTGCTGAACTCATTAACATAAAACCATCTACTTTAA  >  CP000730/2123709‑2123987
                                                                                                                                          |                                                                                                                                            
 aaTCAGTCCTCAACTCTTTTAATGTTTTCATGTTGTCACCGCCTTTCGTAAACCTAATATAATACGAAATTTTCGTATTGTCAACATTAAATACGTTTTTTTCGTAAAAAACTTTACTATGATATGAAAATTTCGTataat                                                                                                                                           >  1:1117522/1‑141 (MQ=255)
 aaTCAGTCCTCAACTCTTTTAATGTTTTCATGTTGTCACCGCCTTTCGTAAACCTAATATAATACGAAATTTTCGTATTGTCAACATTAAATACGTTTTTTTCGTAAAAAACTTTACTATGATATGAAAATTTCGTataat                                                                                                                                           >  1:211170/1‑141 (MQ=255)
   tCAGTCCTCAACTCTTTTAATGTTTTCATGTTGTCACCGCCTTTCGTAAACCTAATATAATACGAAATTTTCGTATTGTCAACATTAAATACGTTTTTTTTTTTTTTTTCTTTACTATGATATGAAAATTTTGTAtaataa                                                                                                                                         >  2:832410/1‑141 (MQ=255)
    cAGTCCTTAACTCTTTAAATGTTGTCATGTTGGCACCGCCTTTCGTAAACCTAATATAATACGAAATTTTCGTATTGGCAACATTAAATACGAAAAAAAAAAAAAAAACTTTACTATGATATGAAAATTTCGTATAATAAg                                                                                                                                        <  2:81476/141‑1 (MQ=255)
    cAGTCCTCAACTCTTTTAATGTTTTCATGTTGTCACCGCCTTTCGTAAACCTAATATAATACGAAATTTTCGTATTGTCAACATTAAATACGTTTTTTTCGTAAAAAACTTTACTATGATATGAAAATTTCGTATAATAAg                                                                                                                                        >  1:477487/1‑141 (MQ=255)
     aGTCCTCAACTCTTTTAATGTTTTCATGTTGTCACCGCCTTTCGTAAACCTAATATAATACGAAATTTTCGTATTGTCAACATTAAATACGTTTTTTTCGTAAAAAACTTTACTATGATATGAAAATTTCGTATAATAAGa                                                                                                                                       >  1:818496/1‑141 (MQ=255)
       tCCTCAACTCTTTTAATGTTTTCATGTTGTCACCGCCTTTCGTAAACCTAATATAATACGAAATTTTCGTATTGTCAACATTAAATACGTTTTTTTTTTTATTTTTTTTACATTGATATGCAAATTTCGTATAATaaaaaa                                                                                                                                     >  2:267624/1‑141 (MQ=255)
       tCCTCAACTCTTTTAATGTTTTCATGTTGTCACCGCCTTTCGTAAACCTAATATAATACGAAATTTTCGTATTGTCAACATTAAATACGTTTTTTTCGTAAAAAACTTTACTATGATATGAAAATTTCGTATAATAAGaaa                                                                                                                                     >  1:155718/1‑141 (MQ=255)
        ccTCAACTCTTTTAATGTTTTCATGTTGTCACCGCCTTTCGTAAACCTAATATAATACGAAATTTTCGTATTGTCAACATTAAATACGTTTTTTTTGTTATAAACTTTACTATGATATGAAAATTTCGTATAATAAGaaaa                                                                                                                                    >  2:675840/1‑141 (MQ=255)
        ccTCAACTCTTTTAATGTTTTCATGTTGTCACCGCCTTTCGTAAACCTAATATAATACGAAATTTTCGTATTGTCAACATTAAATACGTTTTTTTCGTAAAAAACTTTACTATGATATGAAAATTTCGTATAATAAGaaaa                                                                                                                                    >  1:366597/1‑141 (MQ=255)
         cTCAACTCTTTTAATGTTTTCATGTGGTCACCGCCTTTCGTAAACCTAATATAATACGAAATTTTCGTATTGTCAACATTAAATACGTTTTTTTCGTAAAAAACTTTACTATGATATGAAAATTTCGTATAATAAGaaaaa                                                                                                                                   <  1:23822/141‑1 (MQ=255)
            aaCTCTTTTAATGTTTTCATGTTGTCACCGCCTTTCGTAAACCTAATATAATACGAAATTTTCGTATTGTCAACATTAAATACGTTTTTTTTTTTTTTATTTTTACTATGATATGAAAATTTCGTATAATAAGAAAAAagg                                                                                                                                >  2:1108282/1‑141 (MQ=255)
            aaCTCTGTTAATGTTTTTATGTTCTCACCTCCTTTCTTAAACCTAATATAATACGAAATTTTCGTATTGTCAACATTAAATACGTTTTTTTCGTAAAAAACTTTACTATGATATGAAAATTTCGTATAATAAGAAAAAagg                                                                                                                                <  1:561010/141‑1 (MQ=255)
              ctctTTTAATGTTTTCATGTTGTCACCGCCTTTCGTAAACCTAATATAATACGAAATTTTCGTATTGTCAACATTAAATACGTTTTTTTCGTAAAAAACTTTACTATGATATGAAAATTTCGTATAATAAGAAAAaagaag                                                                                                                              >  1:147721/1‑141 (MQ=255)
              ctctTTTAATGTTTTCATGTTGTCACCGCCTTTCGTAAACCTAATATAATACGAAATTTTCGTATTGTCAACATTAAATACGTTTTTTTCGTAAAAAACTTTACTATGATATGAAAATTTCGTATAATAAGAAAAAaggag                                                                                                                              >  1:494709/1‑141 (MQ=255)
              ctctTTTAATGTTTTCATGTTGTCACCGCCTTTCGTAAACCTAATATAATACGAAATTTTCGTATTGTCAACATTAAATACGTTTTTTTCGTAAAAAACTTTACTATGATATGAAAATTTCGTATAATAAGAAAAAaggag                                                                                                                              <  1:533711/141‑1 (MQ=255)
                ctTTTAATGTTTTCATGTTGTCACCGCCTTTCGTAAACCTAATATAGTACGAAATTTTCGTATTGTCAACATTAAATACGATTTTTTCGTAAAATTTCTTATCATGATCTAGAAGTTTAGTGTAATAAGAAACTAGGAGgt                                                                                                                            >  2:385904/1‑141 (MQ=255)
                ctTTTAATGTTTTCATGTTGTCACCGAATTCCGGAAACCTAATATAATACGAAATTTTCGTATTGTCAACATTAAATACGTTTTTTTCGTAAAAAACTTTACTATGATATGAAAATTTCGTATAATAAGAAAAAAGGAGgt                                                                                                                            >  1:284252/1‑141 (MQ=255)
                   ttAATGTTTTCATGTAGACAACGAAATTCGTAAACCTAATATAATACGAAATTTTCGTATTGTCAACATTAAATACGTTTTTTTCGTAATAAACTTTACTATGATATGAAAATTTATTAAAAAAAAAAAAAAGGAGgtaag                                                                                                                         <  2:849856/141‑1 (MQ=255)
                           ttCATGTTGTCACCGCCTTTCGTAAACCTAATATAATACGAAATTTTCGTATTGTCAACATTAAATACGTTTTTTTCGTAAAAAACTTTACTATGATATGAAAATTTCGTATAATAAGAAAAAAGGAGGTAAGTAATATGa                                                                                                                 <  1:31718/141‑1 (MQ=255)
                               tgttgtCACCGCCTTTCGTAAACCTAATATAATACGAAATTTTCGTATTGTCAACATTAAATACGTTTTTTTCGTAAAAAACTTTACTATGATATGAAAATTTCGTATAATAAGAAAAAAGGAGGTAAGTAATATGAACaa                                                                                                             <  1:233144/141‑1 (MQ=255)
                                gttgtCACCGCCTTTCGTAAACCTAATATAATACGAAATTTTCGTATTGTCAACATTAAATACGTTTTTTTCGTAAAAAACTTTACTATGATATGAAAATTTCGTATAATAAGAAAAAAGGAGGTAAGTAATATGAACaaa                                                                                                            >  2:1124758/1‑141 (MQ=255)
                                 ttgtCACCGCCTTTCGTAAACCTAATATAATACGAAATTTTCGTATTGTCAACATTAAATACGTTTTTTTCGTAAAAAACTTTACTATGATCTGAAAATTTCGTCTAATAAGAAAAAAGGAGGTAAGTGATATGAACacag                                                                                                           >  2:288888/1‑141 (MQ=255)
                                 ttgtCACCGCCTTTCGTAAACCTAATATAATACGAAATTTTCGTATTGTCAACATTAAATACGTTTTTTTCGTAAAAAACTTTACTATGATATGAAAATTTCGTATAATAAGAAAAAAGGAGGTAAGTAATATGAACaaag                                                                                                           >  2:498148/1‑141 (MQ=255)
                                 ttgtCACCGCCTTTCGTAAACCTAATATAATACGAAATTTTCGTATTGTCAACATTAAATACGTTTTTTTCGTAAAAAACTTTACTATGATATGAAAATTTCGTATAATAAGAAAAAAGGAGGTAAGTAATATGAACaaag                                                                                                           >  2:372823/1‑141 (MQ=255)
                                  tgtCACCGCCTTTCGTAAACCTAATATAATACGAAATTTTCGTATTGTCAACATTAAATACGTTTTTTTCGTAAAAAACTTTACTATGATATGAAAATTTCGTATAATAAGAAAAAAGGAGGTAAGTAATATGAACaaaga                                                                                                          >  2:1027522/1‑141 (MQ=255)
                                     aaacgaatttCGTAAACCTAATATAATACGAAATTTTCGGATTGTCAACATTAAATACGTTTTTTTCGTAAAAAACTTTACTATGATATGAACATTTCGTAAAAAAAAAAAAAAGGAGGTAAGTAATATGAACaaagaaag                                                                                                       <  2:372396/134‑1 (MQ=255)
                                      aCCGCCTTTCGTAAACCTAATATAATACGAAATTTTCGTATTGTCAACATTAAATACGTTTTTTTTTTTATATTTTTTACTATGATATGAAAATTTCGTATAATAAGAAAAAAGGAGGTAAGTAATATGAACAaagcaaga                                                                                                      >  2:348430/1‑141 (MQ=255)
                                      aCCGCCTTTCGTAAACCTAATATAATACGAAATTTTCGTATTGTCAACATTAAATACGTTTTTTTCGTAAAAAACTTTACTATGATATGAAAATTTCGTATAATAAGAAAAAAGGAGGTTAGTAGTATGAACAaagaaaga                                                                                                      <  1:611031/141‑1 (MQ=255)
                                         gCCTTTCGTAAACCTAATATAATACGAAATTTTCGTATTGTCAACATTAAATACGTTTTTTTCGTAAAAAACTTTACTATGATATGAAAATTTCGTATAATAAGAAAAAAGGAGGAAAGTAATATGAACAAagaaagaact                                                                                                   <  2:158445/141‑3 (MQ=255)
                                          ccTTTCGTAAACCTAATATAATACGAAATTTTCGTATTGTCAACATTAAATACGTTTTTTTCGTAAAAAACTTTACTATGATATGAAAATTTCGTATAATAAGAAACAAGGAGGGAAGTAATATGAACAAAGAAAGAAata                                                                                                  >  1:567269/1‑141 (MQ=255)
                                          ccTTTCGTAAACCTAATATAATACGAAATTTTCGTATTGTCAACATTAAATACGTTTTTTTCGTAAAAAACTTTACTATGATATGAAAATTTCGTATAATAAGAAAAAAGGAGGTAAGTAATATGAACAAAGAAAGAAata                                                                                                  >  1:1052030/1‑141 (MQ=255)
                                            tttCGTAAACCTAATATAATACGAAATTTTCGTATTGTCAACATTAAATACGTTTTTTTCGTAAAAAACTTTACTATGATATTAAAATTTCGTATAATAAGAAAAAAGGAGGTAAGTAATATGAACAAAGAAAGAAAtatt                                                                                                <  1:1060507/141‑1 (MQ=255)
                                             ttCGTAAACCTAATATAATACGAAATTTTCGTATTGTCAACATTAAATACGTTTTTTTCGTAAAAAACTTTACTATGATATGAAAATTTCGTATAATAAGAAAAAAGGAGGTAAGTAATATGAACAAAGAAAGAAAtatta                                                                                               >  1:1069017/1‑141 (MQ=255)
                                             ttCGTAAACCTAATATAATACGAAATTTTCGTATTGTCAACATTAAATACGTTTTTTTCGTAAAAAACTTTACTATGATATGAAAATTTCGTATAATAAGAAAAAAGGAGGTAAGTAATATGAACAAAGAAAGAAAtatta                                                                                               >  1:359886/1‑141 (MQ=255)
                                               cggaagccTAATATAATACAAAATTTTCGTATTGTCAACATTAAATACGTTTTTTTCGTAAAAAACTTTACTATGATATGAAAATTTCGTATAATAAGAAAAAAGGAGGTAAGTAATATGAACAAAGAAAGAAAtattatt                                                                                             >  2:1004213/7‑141 (MQ=255)
                                                 tAAACCTAATATAATACGAAATTTTCGTATTGTCAACATTAAATACGTTTTTTTCGTAAAAAACTTTACTATGATATGAAAATTTCGTATAATAAGAAAAAAGGAGGTCAGTATTATGAACAAAGAAAGAAAtattattat                                                                                           <  1:576814/141‑1 (MQ=255)
                                                    aCCTAATATAATACGAAATTTTCGTATTGTCAACATTAAATACGTTTTTTTCGTAAAAAACTTTACTATGATATGAAAATTTCGTATAATAAGAAAAAAGGAGGTAAGTAATATGAACAAAGAAAGAAATATTATTATAGc                                                                                        <  1:260225/141‑1 (MQ=255)
                                                     ccTAATATAATACGAAATTTTCGTATTGTCAACATTAAATACGTTTTTTTCGTAAAAAACTTTACTATGATATGAAAATTTCGTATAATAAGAAAAAAGGAGGTAAGTAATATGAACAAAGAAAGAAATATTATTATAGcc                                                                                       <  2:499323/141‑1 (MQ=255)
                                                       taatataataCGAAATTTTCGTATTGTCAACATTAAATACGTTTTTTTTTTTTTTTTCTTTACTATGATATGAAAATTTCGTATAATAAGAAAAAAGGAGGTAAGTAATATGAACAAAGAAAGAAATATTATTATAGCCaa                                                                                     >  2:580923/1‑141 (MQ=255)
                                                        aatataataCGAAATTTTCGTATTGTCAACATTAAATACGTTTTTTTCGTAAAAAACTTTACTATGATATGAAAATTTCGTATAATAAGAAAAAAGGAGGTAAGTAATATGAACAAAGAAAGAAATATTATTATAGCCaaa                                                                                    <  1:877939/141‑1 (MQ=255)
                                                         atataataCGAAATTTTCGTATTGTCAACATTAAATACGTTTTTTTCGTAATAAACTTTACTAAGAAATGAAAATTTAATAAAATAAGAAAAAAGGAGGTAAGTACTATGAACAAACAAAGAAATAAGACTAAAGCCaaaa                                                                                   >  2:916141/1‑141 (MQ=255)
                                                         atataataCGAAATTTTCGTATTGTCAACATTAAATACGTTTTTTTCGTAAAAAACTTTACTATGATATGAAAATTTCGTATAATAAGAAAAAAGGAGGTAAGTAATATGAACAAAGAAAGAAATATTATTATAGCCaaaa                                                                                   <  2:154171/141‑1 (MQ=255)
                                                          tataataCGAAATTTTCGTATTGTCAACATTAAATACGTTTTTTTCGTAAAAAACTTTACTATGATATGAAAATTTCGTATAATAAGAAAAAAGGAGGTAAGTAATATGAACAAAGAAAGAAAtattat                                                                                              <  1:1142162/129‑1 (MQ=255)
                                                          aaaaataCGAAATTTTCGGAATGTCAACATTAAATACGTTTTTTTCGTAAAAAACTTTACTATGATATGAAAATTTCGAAAAAAAAAAAAAAAGGAGGTAAGTAATATGAACAAAGAAAGAAATATTATTATAGCCaaaaa                                                                                  <  2:442919/138‑1 (MQ=255)
                                                           ataataCGAAATTTTCGTATTGTCAACATTAAATACGTTTTTTTCGTAAAAAACTTTACTATGATATGAAAATTTCGTATAATAAGAAAAAAGGAGGTAAGTAATATGAACAAAGAAAGAAATATTATTATAGCCAAAAAc                                                                                 >  1:502154/1‑141 (MQ=255)
                                                           ataataCGAAATTTTCGTACTGTCAACATTAAATACGTTTTTTTCGTAAAAAACTTTACTATGATATGAAAATTTCGTATAATAAGAAAAAAGGAGGTAAGTAATATGAACAAAGAAAGAAATATTATTATAGCCAAAAAc                                                                                 <  1:267624/141‑1 (MQ=255)
                                                             aattCGATATTTCAGGATTGTTAACATTAAATACGTTTTTTTCGTAAAAAACTTTACTATGATATGAAAATTTCGTATAATAAGAAAAAAGGAGGTAAGTAATATGAACAAAGAAAGAAATATTATTATAGCCAAAAACAt                                                                               <  1:447161/141‑1 (MQ=255)
                                                              ataCTAAATTTTCGAATTGTCAACATTAAATACGTTTTTTTAGTAAAAAACTTTACTATGATATTAATATTTAAAAAAAAAAAAAAAAAGGAGGTAAGTAATATGAACAAAGAAAGAAATATTATTATAGCCAAAAACAtt                                                                              <  2:233867/141‑1 (MQ=255)
                                                              ataCGAAATTTTCGTATTGTCAACATTAAATACGTTTTTTTCGTAAAAAACTTTACTATGATATGAAAATTTCGTATAATAAGAAAAAAGGAGGTAAGTAATATGAACAAAGAAAGAAATATTATTATAGCCAAAAACAtt                                                                              <  1:1108282/141‑1 (MQ=255)
                                                                aacaaaTCTTCACATTGACAACATTAAAAACGTTTTTATAGTCAAAAACTTTACTATGATATTAAAATTTAAAAAAAAAAAAAAAAAGGAGGTAAGTAATATGAACAAAGAAAGAAATATTATTATAGCCAAAAACATTAg                                                                            <  2:685586/138‑1 (MQ=255)
                                                                aCGAAATTTTCGTATTGTCAACATTAAATACGTTTTTTTCGTAAAAAACTTTACTATGGTATGAAAATTTCGTATAATAAGAAAAAAGGAGGTAAGTAATATGAACAAAGAAAGAAATATTATTATAGCCAAAAACATTAg                                                                            >  1:785923/1‑141 (MQ=255)
                                                                aCGAAATTTTCGTATTGTCAACATTAAATACGTTTTTTTCGTAAAAAACTTTACTATGATATGAAAATTTCGTATAATAAGAAAAAAGGAGGTAAGTAATATGAACAAAGAAAGAAATATTATTATAGCCAAAAACATTAg                                                                            <  1:354461/141‑1 (MQ=255)
                                                                    aaTTTTCGTATTGTCAACATTAAATACGTTTTTTTCGTAAAAAACTTTACTATGATATGAAAATTTCGTATAATAAGAAAAAAGGAGGTAAGTAATATGAACAAAGAAAGAAATATTATTATAGCCAAAAACATTAGaaaa                                                                        >  1:1069689/1‑141 (MQ=255)
                                                                     aTTTTCGTATTGTCAACATTAAATACGTTTTTTTCGTAAAAAACTTTACTATGATATGAAAATTTCGTATAATAAGAAAAAAGGAGGTAAGTAATATGAACAAAGAAAGAAATATTATTATAGCCAAAAACATTAGAAAAt                                                                       >  1:788670/1‑141 (MQ=255)
                                                                      ttttCGTATTGTCAACATTAAATACGTTTTTTTTTTTTTTTTTTTTACTATGATATGAAAATTTTGTATAATAAGAAAAAAGGAGGTAAGTAATATGAACAACGAAATAAATATTATTATTTCTAAAAACATTAGAAAAtt                                                                      >  2:578241/1‑141 (MQ=255)
                                                                      tgttCGTATTGGCAACATTAAATACGTAGTTTTCGTAAAAAACTTTACTATGATATGAAAATTTCGTATAATAAGAAAAAAGGAGGTAAGTAATATGAACAAAGAAAGAAATATTATTATAGCCAAAAACATTAGAAAAtt                                                                      <  2:1044752/139‑1 (MQ=255)
                                                                        ttCGTATTGTCAACATTAAATACGTTTTTTTCGTAAAAAACTTTACTATGATATGAAAATTTCGTATAATAAGAAAAAAGGAGGTAAGTAATATGAACAAAGAAAGAAATATTATTATAGCCAAAAACATTAGAAAATTtc                                                                    <  1:989547/141‑1 (MQ=255)
                                                                         tCGTATTGTCAACATTAAATACGTTTTTTTCGTAAAAAACTTTACTATGATATGAAAATTTCGTATAATAAGAAAAAAGGAGGTAAGTAATATGAACAAAGAAAGAAATATTATTATAGCCAAAAACATTGGAAAATTtcc                                                                   >  1:129892/1‑140 (MQ=255)
                                                                          cGTATTGTCAACATTAAATACGTTTTTTGCGTAAAAAACTTTACTATGATATGAAAATTTCGTATAATAAGAAAAAAGGAGGTAAGTAATATGAACAAAGAAAGAAATATTATTATAGCCAAAAACATTAGAAAATTtctc                                                                  <  1:832410/141‑1 (MQ=255)
                                                                             aTTGTCAACATTAAATACGTTTTTTTTTTTTTTTTCTTTACTATGATAGGAAAATTTCGTAAAATAATAAAAAAGGAGGTAAGTAATATGAACAACGAAAGAAATATTATTATAGCCAAAAACATTAGAAAATTtctttat                                                               >  2:571378/1‑137 (MQ=255)
                                                                             aTTGTCAACATTAAATACGTTTTTTTCGTAAAAAACTTTACTATGATATGAAAATTTCGTATAATAAGAAAAAAGGAGGTAAGTAATATGAACAAAGAAAGAAATATTATTATAGCCAAAAACATTAGAAAATTTCTCAAc                                                               >  1:311158/1‑141 (MQ=255)
                                                                             aTTGTCAACATTAAATACGTTTTTTTCGTAAAAAACTTTACTATGATATGAAAATTTCGTATAATAAGAAAAAAGGAGGTAAGTAATATGAACAAAGAAAGAAATATTATTATAGCCAAAAACATTAGAAAATTTCTCAAc                                                               >  1:62008/1‑141 (MQ=255)
                                                                             aTTGTCAACATTAAATACGTTTTTTTCGTAAAAAACTTTACTATGATATGAAAATTTCGTATAATAAGAAAAAAGGAGGTAAGTAATATGAACAAAGAAAGAAATATTATTATAGCCAAAAACATTAGAAAATTTCTCAAc                                                               <  1:792544/141‑1 (MQ=255)
                                                                               tGTCAACATTAAATACGTTTTTTTCGTAAAAAACTTTACTATGATATGAAAATTTCGTATAATAAGAAAAAAGGAGGTAAGTAATATGAACAAAGAAAGAAATATTATTATAGCCAAAAACATTAGAAAATTTCTCAACGa                                                             <  1:936866/141‑1 (MQ=255)
                                                                                gTCAACATTAAAAACGTTTTTATCGTAAAAAACTTTACTATGATATGAAAATTTCGTATAATAAGAAAAAAGGAGGTAAGTAATATGAACCAAGAACGAAATATTATTATGGCCACAAACATTAGAAAATTTCTCAACGAt                                                            >  1:618960/1‑141 (MQ=255)
                                                                                 tCAACATTAAATACGTTTTTTTCGTAAAAAACTTTACTATGATATGAAAATTTCGTATAATAAGAAAAAAGGAGGTAAGTAATATGAACAAAGAAAGAAATATTATTATAGCCAAAAACATTAGAAAATTTCTCAACGAtt                                                           <  1:548382/141‑1 (MQ=255)
                                                                                   aaCATTAAATACGTTTTTTTCGTAAAAAACTTTACTATGATATGAAAATTTCGTATAATAAGAAAAAAGGAGGTAAGTAATATGAACAAAGAAAGAAATATTATTATAGCCAAAAACATTAGAAAATTTCTCAACGATTCa                                                         <  1:498148/141‑1 (MQ=255)
                                                                                   aaCATTAAATACGTTTTTTTCGTAAAAAACTTTACTATGATATGAAAATTTCGTATAATAAGAAAAAAGGAGGTAAGTAATATGAACAAAGAAAGAAATATTATTATAGCCAAAAACATTAGAAAATTTCTCAACGATTCa                                                         <  1:372823/141‑1 (MQ=255)
                                                                                        tAAAAACATTTTTTTCGCAAAAAACTTTACTAAGATATGAAAATTTCGAAAAAAAAGAAAAAAGGAGGTAAGTAATATGAACAAAGAAAGAAATATTATTATAGCCAAAAACATTAGAAAATTTCTCAACGATTCAAatat                                                    <  2:328253/141‑1 (MQ=255)
                                                                                           atatGTTTTATTAGGAAAAAACTTTACTAGGATATGAAAATTAAAAAAAAAAAAAAAAAAGGAGGTAAGTAATATGAACAAAGAAAGAAATATTATTATAGCCAAAAACATTAGAAAATTTCTCAACGATTCAAATATGtc                                                 <  2:1170416/141‑1 (MQ=255)
                                                                                            tACGTTTTTTTCGTAAAAAACTTTACTATGATATGAAAATTTCGTATAATAAGAAAAAAGGAGGTAAGTAATATGAACAAAGAAAGAAATATTATTATAGCCAAAAACATTAGAAAATTTCTCAACGATTCAAATATGtct                                                <  1:811768/141‑1 (MQ=255)
                                                                                            aaagaaaaaataaaaaaaaaCTTTACTATGATATGATAATTTGGTCTCCTACGTAGTGAGGACGTAAGTAATATGAACAAAGAAAGAAATATTATTATAGCCAAAAACATTAGAAAATTTCTCAACGATTCAAATATGtct                                                <  2:843065/127‑1 (MQ=255)
                                                                                                          aaaaaaCTTTACTATGATATGAAAATTTCGTATAATAAGAAAAAAGGAGGTAAGTAATATGAACAAAGAAAGAAATATTATTATAGCCAAAAACATTAGAAAATTTCTCAACGATTCAAATATGTCTCAAAAGAAACTTGc                                  >  1:1031592/1‑141 (MQ=255)
                                                                                                          aaaaaaCTTTACTATGATATGAAAATTTCGTATAATAAGAAAAAAGGAGGTAAGTAATATGAACAAAGAAAGAAATATTATTATAGCCAAAAACATTAGAAAATTTCTCAACGATTCAAATATGTCTCAAAAGAAACTTGc                                  <  1:433447/141‑1 (MQ=255)
                                                                                                           aaaaaCTTTACTATGATATGAAAATTTCGTATAATAAGAAAAAAGGAGGTAAGTAATATGAACAAAGAAAGAAATATTATTATAGCCAAAAACATTAGAAAATTTCTCAACGATTCAAATATGTCTCAAAAGAAACTTGCt                                 <  1:747360/141‑1 (MQ=255)
                                                                                                              aaCTTTACTATGATATGAAAATTTCGTATAATAAGAAAAAAGGAGGTAAGTAATATGAACAAAGAAAGAAACAACATGTTAGCCAAAAACATTAGAAAATTTCTCAACGATTCAAATATGTCCCAAAAGAAACTTGCTGaa                              >  1:1178487/1‑141 (MQ=255)
                                                                                                               aCTTTACTATGATATGAAAATTTCGTATAATAAGAAAAAAGGAGGTAAGTAATATGAACAAAGAAAGAAATATTATTATAGCCAAAAACATTAGAAAATTTCTCAACGATTCAAATATGTCTCAAAAGAAACTTGCTGAAc                             <  2:895971/141‑1 (MQ=255)
                                                                                                                 tttACTATGATATGAAAATTTCGTATAATAAGAAAAAAGGAGGTAAGTAATATGAACAAAGAAAGAAATATTATTATAGCCAAAAACATTAGAAAATTTCTCAACGATTCAAATATGTCTCAAAAGAAACTTGCTGAACTc                           >  2:215313/1‑141 (MQ=255)
                                                                                                                 tttACTATGATATGAAAATTTCGTATAATAAGAAAAAAGGAGGTAAGTAATATGAACAAAGAAAGAAATATTATTATAGCCAAAAACATTAGAAAATTTCTCAACGATTCAAATATGTCTCAAAAGAAACTTGCTGAACTc                           >  1:455930/1‑141 (MQ=255)
                                                                                                                 tttACTATGATATGAAAATTTCGTATAATAAGAAAAAAGGAGGTAAGTAATATGAACAAAGAAAGAAATATTATTATAGCCAAAAACATTAGAAAATTTCTCAACGATTCAAATATGTATCAAAAGAAAATTGCTGAACTc                           >  2:688315/1‑141 (MQ=255)
                                                                                                                  ttACTATGATATGAAAATTTCGTATAATAAGAAAAAAGGAGGTAAGTAATATGAACAAAGAAAGAAATATTATTATAGCCAAAAACATTAGAAAATTTCTCAACGATTCAAATATGTCTCAAAAGAAACTTGCTGAACTCa                          >  2:1103846/1‑141 (MQ=255)
                                                                                                                  ttACTATGATATGAAAATTTCGTATAATAAGAAAAAAGGAGGTAAGTAATATGAACAAAGAAAGAAATATTATTATAGCCAAAAACATTAGAAAATTTCTCAACGATTCAAATATGTCTCAAAAGAAACTTGCTGAACTCa                          >  2:318816/1‑141 (MQ=255)
                                                                                                                    aCTATGATATGAAAATTTCGTATAATAATAAAAAAGGAGGTAAGTAATATGAACAAAGAAAGAAATATTATTATAGCCAAAAACATTAGAAAATTTTTCAACGATTCAAATATGTCTCAAAAGAAACTTGCTGAACTCAtt                        <  1:675840/141‑1 (MQ=255)
                                                                                                                    aCTATGATATGAAAATTTCGTATAATAAGAAAAAAGGAGGTAAGTAATATCAACAAAGAAAGAAATATTATTATAGCCAAAAACATTAGAAAATTTCTCAACGATTCAAATATGTCTCAAAAGAAACTTGCTGAACTCAtt                        <  2:818496/141‑1 (MQ=255)
                                                                                                                    aCTATGAATTGAAAATTTCGTATAATAAGAAAAAAGGAGGTAAGTAATATGAACAAAGAAAGAAATATTATTATAGCCAAAAACATTAGAAAATTTCTCAACGATTCAAATATGTCTCAAAAGAAACTTGCTGAACTCAtt                        <  1:128498/141‑1 (MQ=255)
                                                                                                                       ataatatgaAAATTTCGTATAATAAGAAAAAAGGAGGTAAGTAATATGAACAAAGAAAGAAATATTATTATAGCCAAAAACATTAGAAAATTTCTCAACGATTCAAATATGTCTCAAAAGAAACTTGCTGAACTCATTAAc                     <  2:489917/138‑1 (MQ=255)
                                                                                                                           tatgaAAATTTCGTATAATAAGAAAAAAGGAGGTAAGTAATATGAACAAAGAAAGAAATATTATTATAGCCAAAAACATTAGAAAATTTCTCAACGATTCAAATATGTCTCAAAAGAAACTTGCTGAACTCATTAACATaa                 <  1:1124758/141‑1 (MQ=255)
                                                                                                                               aaaaTTTCGTATAATAAGAAAAAAGGAGGTAAGTAATATGAACAAAGAAAGAAATATTATTATAGCCAAAAACATTAGAAAATTTCTCAACGATTCAAATATGTCTCAAAAGAAACTTGCTGAACTCATTAACATAAAAcc             >  2:746427/1‑141 (MQ=255)
                                                                                                                               aaaaTTTCGTATAATAAGAAAAAAGGAGGAAAGTAATATGAACAAAGAAAGAAATATTATTATAGCCAAAAACATTAGAAAATTTCTCAACGATTCAAATATGTCTCAAAAGAAACTTGCTGAACTCATTAACATAAAAca             >  2:175438/1‑140 (MQ=255)
                                                                                                                               aaaaTTTCATCTAATAAGAAAAAAGGAGGTAAGTAATATGAACAAAGAAAGAAATATTATTATAGCCAAAAACATTAGAAAATTTCTCAACGATTCAAATATGTCTCAAAAGAAACTTGCTGAACTCATTAACATAAAAcc             <  2:218848/141‑1 (MQ=255)
                                                                                                                                aaaTTTCGTATAATAAGAAAAAAGGAGGTAAGTAATATGAACAAAGAAAGAAATATTATTATAGCCAAAAACATTAGAAAATTTCTCAACGATTCTAATATGTCTCAAAAGAAACTTGCTGAACTCATTAATATAAAACCa            >  2:894657/1‑141 (MQ=255)
                                                                                                                                aaaTTTCGTATAATAAGAAAAAAGGAGGTAAGTAATATGAACAAAGAAAGAAATATTATTATAGCCAAAAACATTAGAAAATTTCTCAACGATTCAAATATGTCTCAAAAGAAACTTGCTGAACTCATTAACATAAAACCa            >  2:608291/1‑141 (MQ=255)
                                                                                                                                     tCGTAAAAGAACAAAAAAGGAGGTAAATAATATGTACAAAGAAAGAAATATTATTAGAGCCAAAAACATTAGAAAATTTCTCAACGATTCAAATATGTCTCAAAAGAAACTTGCTGAACTCATTAACATAAAACCATCtcc       <  2:330696/141‑3 (MQ=255)
                                                                                                                                      cGTATAATAAGAAAAAAGGAGGTAAGTAATATGAACAAAGAAAGAAATATTATTATAGCCAAAAACATTAGAAAATTTCTCAACGATTCAAATATGTCTCAAAAGAAACTTGCTGAACTCATTAACATAAAACCATCTACt      <  2:359886/141‑1 (MQ=255)
                                                                                                                                      agTATAATAAGAAAAAAGGAGGTAAGTAATATGAACAAAGAAAGAAATATTATTATAGCCAAAAACATTAGAAAATTTCTCAACGATTCAAATATGTCTCAAAAGAAACTTGCTGAACTCATTAACATAAAACCATCTACt      <  2:1106388/140‑1 (MQ=255)
                                                                                                                                       gTATAATAAGAAAAAGGGAGGTAAGTAATATGAACAAAGAAAGAAATATTATTATAGCCAAAAACATTAGAAAATTTCTCAACGATTCAAATATGTCTCAAAAGAAACTTGCTGAACGCATTAACATAAAACCATCTACtt     >  1:504295/1‑141 (MQ=255)
                                                                                                                                         ataataAGAAAAAAGGAGGTAAGTAATATGAACAAAGAAAGAAATATTATTATAGCCAAAAACATTAGAAAATTTCTCAACGATTCAAATATGTCTCAAAAGAAACTTGCTGAACTCATTAACATAAAACCATCTACTTTa   <  2:1195209/141‑1 (MQ=255)
                                                                                                                                          taataaGAAAAAAGGAGGTAAGTAATATGAACAAAGAAAGAAATATTATTATAGCCAAAAACATTAGAAAATTTCTCAACGATTCAAATATGTCTCAAAAGAAACTTGCTGAACTCATTAACATAAAACCATCTAATTTaa  >  2:778508/1‑141 (MQ=255)
                                                                                                                                          taataaGAAAAAAGGAGGTAAGTAAGATGAACAAAGAAAGAAATATTATTATAAAAAAAAACATTAGAAAATTTCTCAACGATTCAAATATGTCTCAAAAGAAACTTGCTGAACTCATTAACATAAAACCAGCTACTTTaa  >  1:332324/1‑141 (MQ=255)
                                                                                                                                          |                                                                                                                                            
TAATCAGTCCTCAACTCTTTTAATGTTTTCATGTTGTCACCGCCTTTCGTAAACCTAATATAATACGAAATTTTCGTATTGTCAACATTAAATACGTTTTTTTCGTAAAAAACTTTACTATGATATGAAAATTTCGTATAATAAGAAAAAAGGAGGTAAGTAATATGAACAAAGAAAGAAATATTATTATAGCCAAAAACATTAGAAAATTTCTCAACGATTCAAATATGTCTCAAAAGAAACTTGCTGAACTCATTAACATAAAACCATCTACTTTAA  >  CP000730/2123709‑2123987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: