Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,455,699 A→T 68.4% H406L (CAT→CTT) ‡ USA300HOU_2315 → hypothetical membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,455,6990AT68.4% 161.0 / 119.7 136H406L (CAT→CTT) ‡USA300HOU_2315hypothetical membrane protein
Reads supporting (aligned to +/- strand):  ref base A (25/18);  new base T (44/49);  total (69/67)
Fisher's exact test for biased strand distribution p-value = 2.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.28e-01

Only 100 of 138 total aligned reads displayed.

GAATCAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAATTTTAACACCCTCTT  >  CP000730/2455563‑2455836
                                                                                                                                        |                                                                                                                                         
gAATCAGTTAAACAAGCACCACAAAGTGACGATTTAAAAAACTATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATc                                                                                                                                       <  2:65555/141‑1 (MQ=255)
 aaTCAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCg                                                                                                                                      >  2:1075839/1‑141 (MQ=255)
   tCAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGaaa                                                                                                                                   <  1:668845/141‑1 (MQ=255)
     aGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGaaaa                                                                                                                                  >  2:382043/1‑141 (MQ=255)
     aGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGaaaaa                                                                                                                                 <  2:1051871/141‑1 (MQ=255)
      aatAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACCTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGaaaaat                                                                                                                                <  2:320077/139‑2 (MQ=255)
        aaaaCAAGCACCACAAAGTGCCGATATAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAAATGATGCAAAAAAATTATCGAAAAAACt                                                                                                                              <  2:1054325/140‑1 (MQ=255)
         aaaCAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTg                                                                                                                             <  2:976007/141‑1 (MQ=255)
              aGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTgaagaa                                                                                                                        <  1:5037/141‑1 (MQ=255)
              aGCACCAAAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAAAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTgaagaa                                                                                                                        <  2:270473/141‑1 (MQ=255)
               gCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGGACCTGCGACTTATAAAGAAGACAGACGTTCAGCAGTTTTATTTGATGCAAAAAAATTATCGAAAAAAcgtataaac                                                                                                                       >  1:1146756/1‑133 (MQ=255)
                    acaAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCt                                                                                                                  >  1:210713/1‑141 (MQ=255)
                    acaAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCt                                                                                                                  >  2:950414/1‑141 (MQ=255)
                       aaGTGACGATTTAACAAACGATTCAACTGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGATGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCa                                                                                                               <  1:723515/141‑1 (MQ=255)
                         gTGACGATGTAACGAACGATTCAAAGGAAGATGAAATAGAGGAATCATTAAAAGAACCTGCGAGTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGa                                                                                                             <  2:809519/141‑1 (MQ=255)
                          tGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGAt                                                                                                            <  1:1202121/141‑1 (MQ=255)
                          tGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGAt                                                                                                            <  1:1108520/141‑1 (MQ=255)
                          gcaacaTTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGTGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGAt                                                                                                            <  2:224040/136‑1 (MQ=255)
                           gACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGAAAAAACTGAAGAACAATCTTCAgca                                                                                                            >  2:626699/1‑139 (MQ=255)
                              gATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGAAAAAACTGAAGAACAATCTTCAGATaaa                                                                                                         <  1:100035/141‑1 (MQ=255)
                              gATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACCTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGAAAAAACTGAAGAACAATCTTCAGATaaa                                                                                                         >  2:298780/1‑141 (MQ=255)
                                    aCAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAaat                                                                                                  >  2:351522/1‑141 (MQ=255)
                                       aaCGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAaataa                                                                                                >  1:47206/1‑141 (MQ=255)
                                        aCGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGAAGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTGCAGATGAAAACAAataata                                                                                              >  1:382381/1‑141 (MQ=255)
                                         cGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAAtaataa                                                                                             >  2:1170743/1‑141 (MQ=255)
                                         cGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAataatac                                                                                             >  1:297881/1‑140 (MQ=255)
                                         agATTGAAATGAAGATAAAATAAAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAAAAAAAAAAAAAACTGAAGAACAATCTTCAGATAAAAACAAAtaataa                                                                                             <  2:925442/140‑1 (MQ=255)
                                               aaaTGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAgc                                                                                       >  1:187264/1‑141 (MQ=255)
                                                aaTGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAgcg                                                                                      >  2:811129/1‑141 (MQ=255)
                                                aaTGAAGATGAAATAGAGCAACCATTAAAAGAACCTGCTCCTCATAAAGCAGACCGACAGACATCAGCACATTCGGATGTAAAAAAATCATCGAAAAAACTAAAGAACAATCTTCAGATAAAACCAAAAAAAAACaacacg                                                                                      >  2:400625/1‑137 (MQ=255)
                                                 aTGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAgcg                                                                                      >  2:295897/1‑141 (MQ=255)
                                                    aaGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCtt                                                                                   >  1:1029348/1‑141 (MQ=255)
                                                      gATTAAATAGAGCAATCATTAAAAGAACCTGCGACGTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAACAAACTGCAGAACAATCTTCAGATCAAACCAACTAATAACAAAGCGCTTGCt                                                                                <  2:699278/141‑1 (MQ=255)
                                                      gATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCt                                                                                <  1:941719/141‑1 (MQ=255)
                                                      gATCAAATAGAGCAATCATAAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCACCAGTTGAAATTGATGCAAAAAAAATAACGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCt                                                                                <  2:1095116/141‑1 (MQ=255)
                                                        tGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCATGCTac                                                                              >  2:969635/1‑140 (MQ=255)
                                                          aaaTAGAGCAATCATTAAAAGAACCTGAGACTTATAAAGAAGACAGACGTTCATGGGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATGTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTa                                                                            >  1:996885/1‑141 (MQ=255)
                                                             aagagGAAACATTAAAAGAACCTGCGACTTACAAAGAAGAAAGAAGTTCATCAGTTGTAATTGATGCAGAAAAAAATAAAAAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTAcc                                                                          <  2:27484/140‑1 (MQ=255)
                                                              agagCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACctc                                                                        >  2:649973/1‑141 (MQ=255)
                                                               gagCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACctct                                                                       <  1:898236/141‑1 (MQ=255)
                                                                 gCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGCTGTAATTGATGCAGAAAAACATATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCtt                                                                      >  2:879240/1‑141 (MQ=255)
                                                                  cAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTaa                                                                    >  1:686918/1‑141 (MQ=255)
                                                                   aaTCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTAGCTAGTACCTCTTaa                                                                    >  1:328332/1‑141 (MQ=255)
                                                                    agagatAAAAAAACCTGCGACTTAGAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAACGCGCTTGCTAGTACCTCTTaaaa                                                                  <  2:434281/136‑1 (MQ=255)
                                                                    aTCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTaaa                                                                   <  2:859502/141‑1 (MQ=255)
                                                                    aTCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTaaaa                                                                  >  1:682419/1‑141 (MQ=255)
                                                                     tCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTacaa                                                                  >  1:407308/1‑141 (MQ=255)
                                                                      cATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAg                                                                <  1:1017634/141‑1 (MQ=255)
                                                                           aaaGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAgatga                                                            >  1:217078/1‑141 (MQ=255)
                                                                           aaaGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAgatgat                                                           >  2:818868/1‑141 (MQ=255)
                                                                               aaCCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCt                                                        <  1:720052/141‑1 (MQ=255)
                                                                                aCCTGCGACTTATAAAGAAGACAGACGTTAACCAGTTGTAATTGATGCAGAAAAACATATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGAAGATGCTa                                                       >  1:284669/1‑141 (MQ=255)
                                                                                 ccTGCGACTTCTAAAGAAGACTGACGGTCATCAGTTGGAATGGATGCAAAATAATTATAGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCCCTTGCTAGTACCTCTGAAAAAGATGATGCTAGc                                                     <  2:791186/141‑1 (MQ=255)
                                                                                  cTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCa                                                    <  1:551511/141‑1 (MQ=255)
                                                                                  cTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCa                                                    <  1:140072/141‑1 (MQ=255)
                                                                                   tGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCa                                                    >  2:303358/1‑141 (MQ=255)
                                                                                   tGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCaa                                                   <  2:213822/141‑1 (MQ=255)
                                                                                    gCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAg                                                  >  2:802257/1‑141 (MQ=255)
                                                                                    gCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAg                                                  <  2:904730/141‑1 (MQ=255)
                                                                                    gCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAg                                                  <  1:458724/141‑1 (MQ=255)
                                                                                     cGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAgc                                                 >  1:4143/1‑141 (MQ=255)
                                                                                       aCTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAgcgc                                               <  1:13458/141‑1 (MQ=255)
                                                                                         ttATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCt                                              <  2:407308/141‑1 (MQ=255)
                                                                                           ataAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCatt                                            >  2:459468/1‑141 (MQ=255)
                                                                                           ataAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCtttt                                           >  1:370321/1‑141 (MQ=255)
                                                                                           ataAAGAAGACAGACGTTCATCAGTTGAAAGTGATGCAGAAAAACATATCGAAAAAACTGAATAACAATCTTCAGATAAAAACAAATAAGAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCttt                                            <  2:1131154/141‑1 (MQ=255)
                                                                                             aaaGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCCAGCGCTTTTc                                          >  1:351442/1‑141 (MQ=255)
                                                                                                 aagaCAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAACGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTctatac                                     <  1:1167920/141‑1 (MQ=255)
                                                                                                  agacagacGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTctatact                                    >  1:696761/1‑141 (MQ=255)
                                                                                                     cagacGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTctatacgata                                 >  1:954457/1‑141 (MQ=255)
                                                                                                     cagacGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTGGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTctatactata                                 >  1:588810/1‑141 (MQ=255)
                                                                                                      agacGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACtatat                                <  2:952771/141‑1 (MQ=255)
                                                                                                        acGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGGAGCTAGCAATCGATTTTctatactatttt                               >  1:61889/1‑138 (MQ=255)
                                                                                                         cGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGTTAAAAACAAATAATAACAAAGCGCCTGCTAGTACCACTTAAAAAGATGATGCTAGCAAGGGCTTTTCTATACTATAtatt                             >  2:442408/1‑141 (MQ=255)
                                                                                                           ttCATCAGTTGTAATCGATGCAAAAAAATGATCGAAAAAACTGAAGAACAAGCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTCTACTATAtattat                           >  2:108618/1‑141 (MQ=255)
                                                                                                            tcatcaGTTGTAATTGATGCAGAAAAACATATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATAtattat                           >  1:509156/1‑141 (MQ=255)
                                                                                                            tcatcaGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATattatt                          >  1:581586/1‑141 (MQ=255)
                                                                                                            tcatcaGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACAATATattatt                          >  1:1163455/1‑141 (MQ=255)
                                                                                                             catcaGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATCCTATATATTAttt                         >  2:306880/1‑141 (MQ=255)
                                                                                                              atcaGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCCAGCGCTTTTCTATACTATATATTAtttt                        <  1:240033/141‑1 (MQ=255)
                                                                                                              atcaGTTGTAATTGATGCAAAAAAATTATCGAAAAAAATGAAGAACAATCTTAAGATACAACCAAATAACCATCAACCGCCTGATCGAACCCCTTAAAAAGATGATGCTAACAAGCGCGTTTCTATACTATATATTAtttt                        >  2:694256/1‑141 (MQ=255)
                                                                                                               tcaGTTGTAATTGATGCAGAAAAACATATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATattttatttt                        >  2:220004/1‑141 (MQ=255)
                                                                                                               tcaGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAACGCTTTTCTATACTATATATTAttttt                       >  2:86858/1‑141 (MQ=255)
                                                                                                               tcaGTTGTAATTGATGCAAAAAAATTATCGAAAGAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTAttttt                       <  2:665533/141‑1 (MQ=255)
                                                                                                                 aGTTGTAATTGATGCAGAAAAACATATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTCTAATATATATTATTTTTc                      >  1:447077/1‑141 (MQ=255)
                                                                                                                 aGTTGTAATTGATGCAAAAAAATTATCGAAAAACCTGGAGAAGAATGTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCt                     <  1:1118386/141‑1 (MQ=255)
                                                                                                                   ttGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAACGCGCATTCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTaa                   <  2:1025322/141‑1 (MQ=255)
                                                                                                                   ttGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTaa                   <  1:950414/141‑1 (MQ=255)
                                                                                                                       aaTTGATGCAGAAAAACATATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTACAAt                >  1:344170/1‑141 (MQ=255)
                                                                                                                       aaTTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTCTACTATATATTATTTTTCTAAAAtt               >  2:560539/1‑141 (MQ=255)
                                                                                                                       aaTTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATCAGATATATTATTTTTCTAAAAtt               >  2:918860/1‑141 (MQ=255)
                                                                                                                           tgatgaaaaaaaaTTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACCACGCGCTTGCTAGTGCCTCTGAAAAAGAGGATGCTAGCAATCGCTTTTCTATACTATATATTATTTTTCTAAAATTTTa            <  1:237029/134‑1 (MQ=255)
                                                                                                                              tgcaaaaaaaTTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAATTTTAaca         <  2:1011331/137‑1 (MQ=255)
                                                                                                                              tgcaaaaaaaTTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAATTTTAaca         <  2:59629/137‑1 (MQ=255)
                                                                                                                                 gAAAAACATATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAATTTTAACAccc      >  1:487269/1‑141 (MQ=255)
                                                                                                                                  aaaaaaTTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAATTTTAACACCct     <  2:384229/141‑1 (MQ=255)
                                                                                                                                  aaaaaaTTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAATTTTAACACCct     <  1:818868/141‑1 (MQ=255)
                                                                                                                                  aaaaaaTTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAATTTTAACACCct     <  1:258489/141‑1 (MQ=255)
                                                                                                                                  aaaaaaTTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAATTCTAACACCct     <  1:879069/141‑1 (MQ=255)
                                                                                                                                     aaaatatCGAAAAAACGGAAGAACAATCTACAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAATTTTAACACCCTCtt  <  2:254920/138‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                         
GAATCAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAATTTTAACACCCTCTT  >  CP000730/2455563‑2455836

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: