Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 37,562 A→G 60.7% intergenic (+258/‑251) USA300HOU_0028 → / → ugpQ1 hypothetical protein/glycerophosphodiester phosphodiesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073037,5620AG60.7% 0.4 / 65.4 66intergenic (+258/‑251)USA300HOU_0028/ugpQ1hypothetical protein/glycerophosphodiester phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base A (14/12);  new base G (16/24);  total (30/36)
Fisher's exact test for biased strand distribution p-value = 3.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.81e-01

CAAAATTGCTGATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAACTCCATAATCAATAC  >  CP000730/37422‑37695
                                                                                                                                            |                                                                                                                                     
cAAAATTGCTGATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTa                                                                                                                                       >  1:625281/1‑141 (MQ=255)
    aTTGCTGATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTacaga                                                                                                                                   <  1:479232/141‑5 (MQ=255)
    aTTGCTGATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTacaga                                                                                                                                   >  1:486021/1‑137 (MQ=255)
    aTTGCTGATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATGGCGGATAAGGGGTacaga                                                                                                                                   >  2:353603/1‑137 (MQ=255)
     ttGCTGATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAGAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATGGCTGATAAGGGGTacagaa                                                                                                                                  >  1:6247/1‑136 (MQ=255)
     ttGCTGATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTacagaa                                                                                                                                  >  1:1121189/1‑136 (MQ=255)
      tGCTGATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTacagcaa                                                                                                                                 >  2:388079/1‑135 (MQ=255)
      tGCTGATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTacagaaa                                                                                                                                 >  1:350345/1‑135 (MQ=255)
        cagagaaGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTacagaaaaa                                                                                                                               <  1:544203/136‑9 (MQ=255)
        cTGATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGCGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTacagaaaaa                                                                                                                               <  2:1088932/141‑9 (MQ=255)
        cTGATAAAGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAATGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTacagaaaaa                                                                                                                               <  2:858051/141‑9 (MQ=255)
          gATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGAAGTTCTGGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGAGAAGGGGCAAGTTAAAAga                                                                                                                             >  2:1131275/1‑140 (MQ=255)
             aaGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCGGATAAGGGGTacagaaaaactaga                                                                                                                          >  1:1110228/1‑128 (MQ=18)
                gggTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATCAGCGGTGCGCGAAAACCATGTCGCAGGAAAATTGCTGATAAGGGGTAAGTTAATAGCAATcaa                                                                                                                       >  2:542855/1‑139 (MQ=18)
                    aaGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGAACGTTGAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGt                                                                                                                   <  1:924110/141‑1 (MQ=255)
                           aaGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTg                                                                                                            >  1:1085062/1‑141 (MQ=255)
                            aGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGc                                                                                                           <  2:148679/141‑1 (MQ=255)
                                            aTTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGa                                                                                           <  2:871941/141‑1 (MQ=255)
                                               gCAGATAAGAGGTACGTTACAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAACATTGCAGATAAGGGGTACAGaaaa                                                                                        <  1:763832/141‑1 (MQ=255)
                                                    tgagagGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAg                                                                                   <  1:339767/139‑1 (MQ=255)
                                                              gTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTAc                                                                         >  2:443662/1‑141 (MQ=255)
                                                                    aGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATGGAAGATAAGGGGTACAGAAAAAATAGACTTGATTACAAAATg                                                                   >  2:703843/1‑141 (MQ=255)
                                                                       aGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAg                                                                >  1:196633/1‑141 (MQ=255)
                                                                        gTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGc                                                               <  1:961040/141‑1 (MQ=255)
                                                                            tAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTgg                                                           >  2:831223/1‑141 (MQ=255)
                                                                                  aaaTTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGAACATa                                                     >  2:464066/1‑141 (MQ=255)
                                                                                        cAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGAt                                               <  1:1063185/141‑1 (MQ=255)
                                                                                        cAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGAt                                               <  1:1063025/141‑1 (MQ=255)
                                                                                         aGATAAGAGGTTTGTTAAAAGCAGTTCTAAGTAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGAtt                                              >  2:652303/1‑141 (MQ=17)
                                                                                          gATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGAttt                                             <  1:767122/141‑1 (MQ=11)
                                                                                            tAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGAttttt                                           >  2:1048001/1‑141 (MQ=14)
                                                                                                  gtacGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTaaaaa                                     <  1:532332/138‑1 (MQ=14)
                                                                                                  gtacGTTAAAAGCAATTATAAGAAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTaaaaa                                     <  1:564560/138‑1 (MQ=17)
                                                                                                   tacGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAat                                    <  2:175433/139‑1 (MQ=14)
                                                                                                   tacGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAat                                    <  1:994197/139‑1 (MQ=14)
                                                                                                   tacGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAat                                    <  1:614590/139‑1 (MQ=14)
                                                                                                   tacGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCCGATAAGGCGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAat                                    >  2:302561/3‑141 (MQ=17)
                                                                                                   aacGTTAAAAGCAGTTCTATGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTAAGTAAAATTGCTGATAATGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAat                                    <  2:128871/139‑1 (MQ=18)
                                                                                                     cGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAatga                                  <  2:618485/141‑1 (MQ=17)
                                                                                                     cGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAAGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAACACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAatga                                  <  1:43204/141‑1 (MQ=17)
                                                                                                          aaaGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAatgagatga                             <  1:669179/141‑1 (MQ=17)
                                                                                                           aaGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTGAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTCAAAAatgagatgag                            <  2:817374/141‑1 (MQ=255)
                                                                                                           aaGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCGTGGGACATAAATGATTTTTTAAAAatgagatgag                            >  2:1125588/1‑141 (MQ=17)
                                                                                                             gcagtcctaggcaaaaTTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGAc                          <  1:391338/129‑1 (MQ=18)
                                                                                                             gCAGTTCTCAGTAAAATTGAGGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAACATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGATCTTGGGACATAAATGATTTTTTAAAAAtgagatgagag                          >  1:777342/1‑140 (MQ=255)
                                                                                                             gCAGTTCTCAGTAAAANTGCTGATAAAGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGGTTGGGACATAAATGATTTTTTAAAAATGAGATGAGAc                          <  1:1079534/141‑1 (MQ=255)
                                                                                                             gCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGAc                          <  2:765921/141‑1 (MQ=17)
                                                                                                             gCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGAc                          <  2:1066551/141‑1 (MQ=17)
                                                                                                             gCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGAc                          <  1:283797/141‑1 (MQ=18)
                                                                                                             gCAGTTCTAGGAAAAATTACAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGAAAAAATGCAGATAAGGGGTACAGAAAAACTAGACTTGATGACAAAATCGAGCTTGGGACATAAATAAATAAAAAAAAATGAGATGAGAc                          <  2:336980/141‑1 (MQ=9)
                                                                                                              caattctaggcaaaaTTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACg                         <  2:413388/130‑1 (MQ=18)
                                                                                                              cAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACg                         <  1:1137994/141‑1 (MQ=255)
                                                                                                               aGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGt                        <  1:232150/141‑1 (MQ=17)
                                                                                                                 ttctaggcaaaaTTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAACATGGAGCTTGGGACATAAATGAGTTTTTAAAAAAGAGATGAGACGTAg                      >  1:575860/9‑141 (MQ=17)
                                                                                                                 ttctaggcaaaaTTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATGTTTTAAAAATGAGATGAGACGTAg                      >  1:537995/9‑141 (MQ=17)
                                                                                                                 ttCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAg                      <  1:1031214/141‑1 (MQ=255)
                                                                                                                    taggcaaaaTTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGAtt                   <  1:701528/136‑1 (MQ=18)
                                                                                                                    taggcaaaaTTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGAtt                   >  2:78663/6‑141 (MQ=18)
                                                                                                                    taggcaaaaTTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGAtt                   >  2:1140313/6‑141 (MQ=18)
                                                                                                                     cAGTAAAATTGCTGATAAGGGGTAAGCAAAAAGCAAGCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTa                  >  1:576908/1‑141 (MQ=255)
                                                                                                                     aggcaaaaTTGCAGATAAGAGGTGCGTTACAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTGTTTAAAAATGAGATGAGACGTAGATTa                  >  2:871345/5‑141 (MQ=21)
                                                                                                                      ggcaaaaTTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTaa                 >  1:503498/4‑141 (MQ=21)
                                                                                                                      ggcaaaaTTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTaa                 <  2:350345/138‑1 (MQ=21)
                                                                                                                      ggcaaaaTTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTaa                 >  1:33476/4‑141 (MQ=21)
                                                                                                                      ggcaaaaTTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTACGTCCCATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTaa                 <  1:388079/138‑1 (MQ=25)
                                                                                                                      aGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGCTGAGACGTGGATTaa                 >  1:563831/1‑141 (MQ=255)
                                                                                                                       gcaaaaTTGCAGATAAGAGGTGCGTTAAACGCCGTTCTCAGTAAAAGTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAAc                <  1:513331/139‑1 (MQ=21)
                                                                                                                       gcaaaaTTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACTGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAAc                <  1:806925/139‑1 (MQ=21)
                                                                                                                       gaaaaaTTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAAc                <  2:83345/139‑1 (MQ=21)
                                                                                                                          aaaTTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAACTcc             >  1:993152/1‑141 (MQ=255)
                                                                                                                          aaaTTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAACTcc             >  2:754367/1‑141 (MQ=21)
                                                                                                                             ttGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAACTCCATa          >  1:944926/1‑141 (MQ=25)
                                                                                                                                cagATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAACTCCATAATc       >  1:410778/3‑141 (MQ=25)
                                                                                                                                 agATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTAGAAAGTCATCCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAACTCCATAATCa      >  1:567847/2‑141 (MQ=21)
                                                                                                                                 agATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAACTCCATAATCa      >  1:462897/2‑141 (MQ=25)
                                                                                                                                  gATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAACTCCATAATCaa     <  1:781819/141‑1 (MQ=25)
                                                                                                                                     aaGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATGAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAACTACATAATCAATaa  >  2:683203/1‑140 (MQ=255)
                                                                                                                                     aaGGGGTAAGTTAAAAGCAATACTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAACTCCATAATCAATAc  >  1:622730/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                     
CAAAATTGCTGATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAACTCCATAATCAATAC  >  CP000730/37422‑37695

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: