Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 37,563 A→C 60.6% intergenic (+259/‑250) USA300HOU_0028 → / → ugpQ1 hypothetical protein/glycerophosphodiester phosphodiesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073037,5630AC60.6% ‑3.3 / 68.8 66intergenic (+259/‑250)USA300HOU_0028/ugpQ1hypothetical protein/glycerophosphodiester phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base A (14/12);  new base C (16/24);  total (30/36)
Fisher's exact test for biased strand distribution p-value = 3.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.47e-01

GATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAACTCCATAATCAATAC  >  CP000730/37432‑37695
                                                                                                                                   |                                                                                                                                    
gATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGAAGTTCTGGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGAGAAGGGGCAAGTTAAAAga                                                                                                                             >  2:1131275/1‑140 (MQ=255)
      gggTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATCAGCGGTGCGCGAAAACCATGTCGCAGGAAAATTGCTGATAAGGGGTAAGTTAATAGCAATcaa                                                                                                                       >  2:542855/1‑139 (MQ=18)
          aaGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGAACGTTGAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGt                                                                                                                   <  1:924110/141‑1 (MQ=255)
                 aaGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTg                                                                                                            >  1:1085062/1‑141 (MQ=255)
                  aGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGc                                                                                                           <  2:148679/141‑1 (MQ=255)
                                  aTTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGa                                                                                           <  2:871941/141‑1 (MQ=255)
                                     gCAGATAAGAGGTACGTTACAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAACATTGCAGATAAGGGGTACAGaaaa                                                                                        <  1:763832/141‑1 (MQ=255)
                                          tgagagGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAg                                                                                   <  1:339767/139‑1 (MQ=255)
                                                    gTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTAc                                                                         >  2:443662/1‑141 (MQ=255)
                                                          aGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATGGAAGATAAGGGGTACAGAAAAAATAGACTTGATTACAAAATg                                                                   >  2:703843/1‑141 (MQ=255)
                                                             aGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAg                                                                >  1:196633/1‑141 (MQ=255)
                                                              gTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGc                                                               <  1:961040/141‑1 (MQ=255)
                                                                  tAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTgg                                                           >  2:831223/1‑141 (MQ=255)
                                                                        aaaTTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGAACATa                                                     >  2:464066/1‑141 (MQ=255)
                                                                              cAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGAt                                               <  1:1063185/141‑1 (MQ=255)
                                                                              cAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGAt                                               <  1:1063025/141‑1 (MQ=255)
                                                                               aGATAAGAGGTTTGTTAAAAGCAGTTCTAAGTAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGAtt                                              >  2:652303/1‑141 (MQ=17)
                                                                                gATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGAttt                                             <  1:767122/141‑1 (MQ=11)
                                                                                  tAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGAttttt                                           >  2:1048001/1‑141 (MQ=14)
                                                                                        gtacGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTaaaaa                                     <  1:532332/138‑1 (MQ=14)
                                                                                        gtacGTTAAAAGCAATTATAAGAAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTaaaaa                                     <  1:564560/138‑1 (MQ=17)
                                                                                         tacGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAat                                    <  2:175433/139‑1 (MQ=14)
                                                                                         tacGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAat                                    <  1:994197/139‑1 (MQ=14)
                                                                                         tacGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAat                                    <  1:614590/139‑1 (MQ=14)
                                                                                         tacGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCCGATAAGGCGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAat                                    >  2:302561/3‑141 (MQ=17)
                                                                                         aacGTTAAAAGCAGTTCTATGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTAAGTAAAATTGCTGATAATGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAat                                    <  2:128871/139‑1 (MQ=18)
                                                                                           cGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAatga                                  <  2:618485/141‑1 (MQ=17)
                                                                                           cGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAAGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAACACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAatga                                  <  1:43204/141‑1 (MQ=17)
                                                                                                aaaGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAatgagatga                             <  1:669179/141‑1 (MQ=17)
                                                                                                 aaGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTGAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTCAAAAatgagatgag                            <  2:817374/141‑1 (MQ=255)
                                                                                                 aaGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCGTGGGACATAAATGATTTTTTAAAAatgagatgag                            >  2:1125588/1‑141 (MQ=17)
                                                                                                   gcagtcctaggcaaaaTTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGAc                          <  1:391338/129‑1 (MQ=18)
                                                                                                   gCAGTTCTCAGTAAAATTGAGGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAACATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGATCTTGGGACATAAATGATTTTTTAAAAAtgagatgagag                          >  1:777342/1‑140 (MQ=255)
                                                                                                   gCAGTTCTCAGTAAAANTGCTGATAAAGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGGTTGGGACATAAATGATTTTTTAAAAATGAGATGAGAc                          <  1:1079534/141‑1 (MQ=255)
                                                                                                   gCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGAc                          <  2:1066551/141‑1 (MQ=17)
                                                                                                   gCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGAc                          <  2:765921/141‑1 (MQ=17)
                                                                                                   gCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGAc                          <  1:283797/141‑1 (MQ=18)
                                                                                                   gCAGTTCTAGGAAAAATTACAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGAAAAAATGCAGATAAGGGGTACAGAAAAACTAGACTTGATGACAAAATCGAGCTTGGGACATAAATAAATAAAAAAAAATGAGATGAGAc                          <  2:336980/141‑1 (MQ=9)
                                                                                                    caattctaggcaaaaTTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACg                         <  2:413388/130‑1 (MQ=18)
                                                                                                    cAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACg                         <  1:1137994/141‑1 (MQ=255)
                                                                                                     aGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGt                        <  1:232150/141‑1 (MQ=17)
                                                                                                       ttctaggcaaaaTTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAACATGGAGCTTGGGACATAAATGAGTTTTTAAAAAAGAGATGAGACGTAg                      >  1:575860/9‑141 (MQ=17)
                                                                                                       ttctaggcaaaaTTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATGTTTTAAAAATGAGATGAGACGTAg                      >  1:537995/9‑141 (MQ=17)
                                                                                                       ttCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAg                      <  1:1031214/141‑1 (MQ=255)
                                                                                                          taggcaaaaTTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGAtt                   >  2:78663/6‑141 (MQ=18)
                                                                                                          taggcaaaaTTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGAtt                   >  2:1140313/6‑141 (MQ=18)
                                                                                                          taggcaaaaTTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGAtt                   <  1:701528/136‑1 (MQ=18)
                                                                                                           cAGTAAAATTGCTGATAAGGGGTAAGCAAAAAGCAAGCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTa                  >  1:576908/1‑141 (MQ=255)
                                                                                                           aggcaaaaTTGCAGATAAGAGGTGCGTTACAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTGTTTAAAAATGAGATGAGACGTAGATTa                  >  2:871345/5‑141 (MQ=21)
                                                                                                            ggcaaaaTTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTaa                 <  2:350345/138‑1 (MQ=21)
                                                                                                            ggcaaaaTTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTaa                 >  1:33476/4‑141 (MQ=21)
                                                                                                            ggcaaaaTTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTaa                 >  1:503498/4‑141 (MQ=21)
                                                                                                            ggcaaaaTTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTACGTCCCATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTaa                 <  1:388079/138‑1 (MQ=25)
                                                                                                            aGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGCTGAGACGTGGATTaa                 >  1:563831/1‑141 (MQ=255)
                                                                                                             gcaaaaTTGCAGATAAGAGGTGCGTTAAACGCCGTTCTCAGTAAAAGTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAAc                <  1:513331/139‑1 (MQ=21)
                                                                                                             gcaaaaTTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACTGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAAc                <  1:806925/139‑1 (MQ=21)
                                                                                                             gaaaaaTTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAAc                <  2:83345/139‑1 (MQ=21)
                                                                                                                aaaTTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAACTcc             >  1:993152/1‑141 (MQ=255)
                                                                                                                aaaTTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAACTcc             >  2:754367/1‑141 (MQ=21)
                                                                                                                   ttGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAACTCCATa          >  1:944926/1‑141 (MQ=25)
                                                                                                                      cagATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAACTCCATAATc       >  1:410778/3‑141 (MQ=25)
                                                                                                                       agATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTAGAAAGTCATCCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAACTCCATAATCa      >  1:567847/2‑141 (MQ=21)
                                                                                                                       agATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAACTCCATAATCa      >  1:462897/2‑141 (MQ=25)
                                                                                                                        gATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAACTCCATAATCaa     <  1:781819/141‑1 (MQ=25)
                                                                                                                           aaGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATGAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAACTACATAATCAATaa  >  2:683203/1‑140 (MQ=255)
                                                                                                                           aaGGGGTAAGTTAAAAGCAATACTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAACTCCATAATCAATAc  >  1:622730/1‑141 (MQ=255)
                                                                                                                                   |                                                                                                                                    
GATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAACTCCATAATCAATAC  >  CP000730/37432‑37695

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: