Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	1,233,504	T→A	V470D (GTT→GAT)	prkC →	non‑specific serine/threonine protein kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,233,504	0	T	A	100.0%	60.4 / NA	21	V470D (GTT→GAT)	prkC	non‑specific serine/threonine protein kinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (6/15);  total (6/15)

AACGTGTTGAACGTGGTGACAGTGTTGATGTTGTTATATCAAAGGGCCCTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGTTACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTAATATTAAACTATATGAATCTTTAGGCATT  >  CP000730/1233372‑1233628                                                                                                                                     |                                                                                                                             aaCGTGTTGAACGTGGTGACAGTGTTTATGCTGTTATATCAAAGGGACCTGAAAAGGTTAAAACGCCAAATGTCATTGGTTTACCTAAGGAGTAAGCCTTGCAGAAAATAAAATCGTTAGGTCCTAAAGCTGATACGATTg                                                                                                                      <  2:188471/141‑1 (MQ=255)   cgtatcgAACGTGGAGACAGGGATCAAGGTGTTAAAGCAAAGGGCCCTGAAAAGGTAAAGATGACAAACGTCATTGGTTTACCTAAGGAGGCAGCCTTGCAGAACTTAAAATCGTTAGGTCTTAAAGATGATACGAATGaa                                                                                                                    >  2:118185/7‑141 (MQ=255)                   aCAGTGTTGATGTTGTTATATCAAAGGGCCCTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATGATc                                                                                                    >  2:349540/1‑141 (MQ=255)                      attttGATGTTGTTATTTAAAATGTCCCTCAAAAGGTTAAAATGCCAAATTTCATTTGTTTACCTAATTAAGAAGCCTTGCAGAAATTAAAATACTTAAGTCTTAAAGATGATACGATTGAAAAATTATATAATAATCAAg                                                                                                 <  2:76832/138‑1 (MQ=255)                                     tgtCAAAGGGCCCTGAAAAGTTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACa                                                                                  >  2:493889/3‑141 (MQ=255)                                              gCCCTGAAAAGGTTAAAATGCCAAATGTCACGGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATc                                                                         >  2:83805/1‑141 (MQ=255)                                                tcTGATAAGGTTAAAATGCCAAATGTCATTGGTTTTCTTAAGGAGGAAGCCTTGCAGTAATTATAATCGTTTGGTCTTAATGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCaa                                                                       <  2:385327/140‑1 (MQ=255)                                                ccTGAAAAGGTTAAAATGCCAAATGTCATAGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAAACGGTAGGTCTTAAAGATGATACGATGGAAAAAGTATATAATAAACAAACGCCAAAAGGATCCGTTGCAAATCaa                                                                       >  2:7055/1‑141 (MQ=255)                                                       agattAAAATGACAAATGTCAATGGTTTACCTAAGCAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGAAAAAAAAAAAAAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTaa                                                                <  2:178691/138‑1 (MQ=255)                                                                  ccAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACt                                                     <  2:425180/141‑1 (MQ=255)                                                                  ccAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAAACGCAAATACt                                                     >  2:352216/1‑141 (MQ=255)                                                                        ttCATTGGTTTACCTAAGGAAGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGAAGATACAATTAAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATc                                               <  2:218941/140‑1 (MQ=255)                                                                             tGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTAt                                          <  2:279001/141‑1 (MQ=255)                                                                                         ggaggaAGCCTTGCAGAAATTAAACTCGCTTGGCTGTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAACTCCGGGACCGCGACTCATGATTCTaa                              <  2:224074/141‑1 (MQ=255)                                                                                           aggaAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTAata                            <  2:452977/141‑1 (MQ=255)                                                                                               aGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAACGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTAATATTaa                        <  2:209098/141‑1 (MQ=255)                                                                                                     gCAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTAATATTAAACtata                  <  2:102841/141‑1 (MQ=255)                                                                                                     gCAAAAATTAAAATCGTGAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAGGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTAATATTAAACtata                  <  2:44255/141‑1 (MQ=255)                                                                                                      cAGAAATTAAAATCGTTAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTAATATTAAACTatat                 <  2:332749/141‑1 (MQ=255)                                                                                                         aaaTTAAAATCGTTAGATCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTAATATTAAACTATATGaa              <  2:455784/141‑1 (MQ=255)                                                                                                                     ttAGGTCTTAAAGATGATACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTAATATTAAACTATATGAATCTTTAGGCAtt  <  2:12587/141‑1 (MQ=255)                                                                                                                                     |                                                                                                                             AACGTGTTGAACGTGGTGACAGTGTTGATGTTGTTATATCAAAGGGCCCTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAGAAATTAAAATCGTTAGGTCTTAAAGATGTTACGATTGAAAAAGTATATAATAATCAAGCGCCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGATTCTAATATTAAACTATATGAATCTTTAGGCATT  >  CP000730/1233372‑1233628

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑