Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	1,744,362	G→A	H104Y (CAC→TAC)	apt ←	adenine phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,744,362	0	G	A	100.0%	42.3 / NA	15	H104Y (CAC→TAC)	apt	adenine phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/11);  total (4/11)

TGCAATACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCAC  >  CP000730/1744237‑1744499                                                                                                                              |                                                                                                                                          tGCAATACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTTTACATTGTTAAAACAtt                                                                                                                            >  2:433140/1‑141 (MQ=255)    aaTACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGt                                                                                                                         <  2:294692/141‑1 (MQ=255)         ctactaCGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCat                                                                                                                    <  2:27282/141‑1 (MQ=255)                            tttttCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTCGGTCATACTCATa                                                                                                 >  2:458946/1‑141 (MQ=255)                                              tgctgcTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAgg                                                                               <  2:283157/141‑1 (MQ=255)                                              tgctgcCTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAgg                                                                               <  2:393960/141‑1 (MQ=255)                                                   ctTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTAGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAAtt                                                                          <  2:265192/141‑1 (MQ=255)                                                      cagtCGTACCACCGGTAGCTAATCAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTCGTCACATATTCTAGCTCATACTCATAACGAATGACTTCACGAGGTAATTTcc                                                                       <  2:454062/138‑1 (MQ=255)                                                       aaTCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTccc                                                                      <  2:354539/141‑1 (MQ=255)                                                                                tcatcTGTAATTAACGCACGTTGTGCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCGTTCTTTTCTAACAGGTGCAAAGCCa                                             <  2:205333/141‑1 (MQ=255)                                                                                             acacacGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAAt                                <  2:308795/141‑1 (MQ=255)                                                                                                cacGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGTTGCAAAGCCAATCACCATTGCATagg                             >  2:85083/1‑139 (MQ=255)                                                                                                          tGGTTTACTTTCACCTGTGTACAGTGTTAAAACATCTTTACCATATTCGAGGTCATACTCATAACGACTGACTCGCCGAGGTAATTTCCCTTCTTTTCTAACACGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACa                   <  2:60737/141‑1 (MQ=255)                                                                                                             tttAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGcc                <  2:212977/141‑1 (MQ=255)                                                                                                                   tGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGaa          >  2:487716/1‑140 (MQ=255)                                                                                                                           tgttCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTACAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATACTAAAGCCAc  >  2:329308/1‑141 (MQ=255)                                                                                                                              |                                                                                                                                          TGCAATACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCAC  >  CP000730/1744237‑1744499

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑