| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP000730 | 1,963,768 | C→G | V365L (GTA→CTA) | hemG ← | protoporphyrinogen oxidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 1,963,768 | 0 | C | G | 89.5% | 45.3 / 0.8 | 20 | V365L (GTA→CTA) | hemG | protoporphyrinogen oxidase |
| Reads supporting (aligned to +/- strand): ref base C (2/0); new base G (6/11); total (8/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.64e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.68e-01 | |||||||||||
TTTCGGCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTAT > CP000730/1963634‑1963893 | tttCGGCAAACGATTGACAATTGTAAATTAAGGATCACCTTTAAATGTCATCATTTGAATTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTAACTAGATACGc > 2:35874/1‑141 (MQ=255) tGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTc < 2:384238/141‑1 (MQ=255) gTAAATTCAGGCTCACCGTTAAATGTTATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTcc < 2:464901/141‑1 (MQ=255) ggATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTCCCTTCCGGTgttgta > 2:110761/1‑141 (MQ=255) aTCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTaaa < 2:279637/141‑1 (MQ=255) cACCTTAAAATGTCAAAATATGACTTAAAGCTCTACGTACAATCGATACTAATTCATGATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATg > 2:44695/1‑141 (MQ=255) cTTAAAATGGCATCATTTGACTTAAATCGCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGcc < 2:499017/141‑1 (MQ=255) tcatccTTTCACTTAAGTCTCTACGTACAATCTATACTACTTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCg < 2:423038/141‑1 (MQ=255) ctctTCGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCgg < 2:89955/141‑1 (MQ=255) gATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGCTGTCCAAGTACATCCGGTAATGTCTGTATCa > 2:111441/1‑141 (MQ=255) gATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCa < 2:267370/141‑1 (MQ=255) aTACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTCCCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCTTTTTTTCTATGTCCAAGTACATGCGGTAATGTCTGTATCAc < 2:116937/141‑1 (MQ=255) cATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCg > 1:5085/1‑141 (MQ=255) gTATGATGATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAAGCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACg < 2:65660/141‑1 (MQ=255) gATCAACAACCACAGTATCACCTGGTTTACCTAAATACGCACGAATCAAAACCTTACCTTCCGGCGTAGTAAATGGCCAGTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGc < 2:26274/141‑1 (MQ=255) atcaACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGACCTTTTTTCGGTCTCCAAGTACATGCGGTCATGCTCGTATCACTCGTTCTCGCAATTACGAAGCCAGt < 2:478103/141‑1 (MQ=255) tcaACCACAGTATCACCTGGTTTACCTACATACGCACGAATAAAAACCTTACCTTCCGGTGTAGTGAATGGCCATTTTTTCGATGTCCAAGAACATGTGGTAATGTCTGTATCACTCTCTATTGCATTTACGAAGGAAGTa > 1:8176/1‑141 (MQ=255) aCCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCAAGTACca > 2:190361/1‑141 (MQ=255) aTCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGt < 2:43880/141‑1 (MQ=255) cACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTAt > 2:8049/1‑141 (MQ=255) | TTTCGGCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTAT > CP000730/1963634‑1963893 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |