Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	76,969	G→A	intergenic (+1027/‑107)	USA300HOU_0072 → / → USA300HOU_0075	universal stress protein/lysophospholipase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	76,969	0	G	A	100.0%	49.1 / NA	21	intergenic (+1027/‑107)	USA300HOU_0072/USA300HOU_0075	universal stress protein/lysophospholipase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/7);  total (14/7)

TCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATTATATAAAATTTATTGAGT  >  CP000730/76831‑77100                                                                                                                                           |                                                                                                                                    tctTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAagg                                                                                                                                   >  2:407426/1‑141 (MQ=255)                     ccccATGCCACGAAATTAGAATCAGGCAAGCAAGTTAATCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACt                                                                                                               <  2:244957/139‑1 (MQ=18)                      gCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTg                                                                                                              >  2:308335/1‑141 (MQ=255)                                    ttAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAAAATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGGTTTg                                                                                                >  2:150137/1‑141 (MQ=255)                                        catcatGCGAGCAAATAAAGCTAACACTCACATAGTAAATTAATTGATAGCTATTTTTTTTTACTTTGAAACAGAACCTATAAAAAACATCGGCTTAAAAGTTTAATTTATATAGTATTACTGCAAACATTAATTTGTGTc                                                                                            <  2:269553/141‑1 (MQ=255)                                                  gCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAgg                                                                                  >  2:12914/1‑141 (MQ=255)                                                         aaGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTa                                                                           >  2:415300/1‑141 (MQ=255)                                                         aaGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTa                                                                           >  2:415246/1‑141 (MQ=255)                                                                aactaaaaagaaaaaTGAGTGGTTAGATATATGTTTTTACATTGCAACAGAACCTAGAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTAAAAAAAAAAAAAAATTAGGGGTATTAAAAAaat                                                                    <  2:71381/129‑1 (MQ=255)                                                                                tAGTGGTTAGCTATATTTTTTTATTTTGCAGCAGTACCTATGAAAAACATGGTCTTTATAGGTTAATTTATATGGTATTAGTGCAAACATTGATTTGGGTCAGAAATTAGGGGTATTAAAAAaatgaatgtttgttaattt                                                    >  2:336520/1‑130 (MQ=255)                                                                                tAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAATATTAGGGGTATTAAAAAAATGAATGAATTATAtttt                                                    >  2:13430/1‑141 (MQ=255)                                                                                 aGTGGTTAGCTATATTTTTTTTTTTTTTTACAGAACCAATAAAAAACATGGGCTTAAAAGTTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATTAATGATTCTTAAtttt                                                   >  2:36633/1‑141 (MQ=255)                                                                                  gTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGGATGAATCATATttttt                                                  >  2:242428/1‑140 (MQ=255)                                                                                          cTATAGTTTTTTACTTTGCGACAGAGAGTAGAAAAAACATGGGGTTAAAAGGTGAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAAAGAAAAAAAAAAAATGAATGAATCATAATTTTATCAAGCTg                                          <  2:18974/141‑1 (MQ=255)                                                                                           tataTTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGa                                         <  2:249229/141‑1 (MQ=255)                                                                                            atatTTTTTTATTTTGCAACAGAACCTATAAAAACCATGGGCTTAACAGGTTAATTTATATAGTATTACGGCAAACATTGATTTGTGTCAAAAATTATGGGTATTAAAAAAATAAATGAATCATAATATTACCAAGCTGAt                                        >  2:404597/1‑141 (MQ=255)                                                                                                  ttttACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATACTTTTATCAAGCTATTTGgagg                                  >  2:62071/1‑140 (MQ=255)                                                                                                        tttGCAACATAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATGAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTaa                            <  2:99694/141‑1 (MQ=255)                                                                                                         ttGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGAATGGAGAGGTTaaa                           >  2:388971/1‑141 (MQ=255)                                                                                                              aacgcaCCTATAAAAAACATGGACTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAACGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCa                      <  2:317634/136‑1 (MQ=255)                                                                                                                     cTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATtatata               >  2:204288/1‑141 (MQ=255)                                                                                                                                  gggCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTCGGGTCAAAAATTAGGGGAATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATTATATACAATTTTTTGAGt  >  2:104980/1‑141 (MQ=255)                                                                                                                                           |                                                                                                                                    TCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATTATATAAAATTTATTGAGT  >  CP000730/76831‑77100

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑