| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP000730 | 2,028,652 | C→T | intergenic (‑8/+7) | yvqF ← / ← USA300HOU_1883 | hypothetical membrane protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 2,028,652 | 0 | C | T | 93.3% | 31.5 / NA | 15 | intergenic (‑8/+7) | yvqF/USA300HOU_1883 | hypothetical membrane protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (6/8); total (7/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.67e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGTTTTTTCTGTTGCTATTTTTTCAGTTGAAGTTTGTTGAATTTGAGTTTCAATTTGTGCATAAT > CP000730/2028518‑2028759 | aTACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAGATGATAAACATTTGCGTTGATATATATTTGTATGTCCTGTTTGGCTGCCttt > 2:486651/1‑141 (MQ=255) ccAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAAATGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTACGTTGATATATATTTGTATGTCATTTTTCGCTGCCTTTCTATAccca > 2:218165/1‑139 (MQ=255) ataataGAACGAGTAAAAAGCCAATTTTTTTTTTTTTTTTTTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAATTGATCAACATTTGCGTTGATATCTATTTTTGTGTCCTGTTTTGCTGCCTTTCTATGCCACAGc > 2:482014/1‑141 (MQ=255) gAACGAGTAAAAAGCCAATTTTTTTATATATTTTTTAGTCAAAATAGGCAATAATCATTAATCCAGTAAAAATGAACAACACTTGCGTTGCCATACAATTGTCTGTCACGTTTCCCCGCCCAACTACGCCACATCGTTCat > 2:7507/1‑141 (MQ=255) aCGAGTAAAAAGCAAATTTTTTAAAAAAATATGTAGTAAAAATTGGTAATAATCATTAATGCAGTACAAATGATCACCATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCTGCCTTTCTATGCCACAGCGTTCatat < 2:374947/141‑1 (MQ=255) aaaaGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTACTGCAGTAAAAATGATAAACATTTGCGTTAATATATATTTATGTGTCATGTTTCACTGCCGTTCTATGCCACAGCGTTCATATAAttttt > 2:342295/1‑139 (MQ=255) aattcttCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCTGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACg < 2:369963/136‑1 (MQ=255) tttCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCTGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGt < 2:359759/141‑1 (MQ=255) aaaaTTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCTGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGTTTTTCCTGTTGCTAttttt < 2:36651/141‑1 (MQ=255) aTTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCTGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGTTTTTTCTGTTGCTATTTTTTCa > 2:205785/1‑141 (MQ=255) taacactgttCACCATTTGCGTTGATATCTACTTGTGTGGCAGGTTTCCCTGCCTTTCCATGCCACAGCGTTCATATAATTTATTAGATACTATGTTTTTTCTGTTGCTATTTTTTCAGTTGAAGTTTGTTGAATTTGAGt < 2:61680/132‑1 (MQ=255) aaaaTGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCTGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGTTTTTTCTGTTGCTATTTTTTCAGTTGAAGTTTGTTGAATTTGAGttt < 2:164957/141‑1 (MQ=255) aaaTGATCAATCTTTGCGTTTATATATATTTGTGTGTCATGTTTCGCTGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGTTTTTTCTGTTGCTATTTTTTCAGTTGAAGTTTGTTGAATTTGAGTTTc < 2:434359/141‑1 (MQ=255) aaaTGATCAACATTTGCGTTGATATATACTTGTGTGTCATGTTTCGCTGCCTTCCTAAGGCACAGTGTTCCTATAAGTTTTTTGATACGATGTTTTTTCTGTTGCTATTTTTTTAGTTGAAGTTTGTTGAATTTGAGTTTc < 2:123660/141‑1 (MQ=255) tGCGTTGATATATATTTGTGTGTCATGTTTCGCTGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGTTGTTTTTACTGTTGCTATTTTTTCTGTTGAAGTTTGTTGAATTTGAGTTTCAATTTGTGCataat > 2:11258/1‑141 (MQ=255) | ATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATATAATTTATTAGATACGATGTTTTTTCTGTTGCTATTTTTTCAGTTGAAGTTTGTTGAATTTGAGTTTCAATTTGTGCATAAT > CP000730/2028518‑2028759 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |