Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	2,184,430	G→T	T176K (ACA→AAA)	yhgF ←	S1 domain RNA‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,184,430	0	G	T	100.0%	45.3 / NA	16	T176K (ACA→AAA)	yhgF	S1 domain RNA‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (9/7);  total (9/7)

AAACTCTATGATTAGCAATGCGTTTAATTGGCTCACTATATGCATAGTACATTTCAAAAATACCTTTTTCATCTTCAGCATTTTTCTTTTTAGATGTAGTTAACACACCTTGATGATACATATCTTTTAAAATTTTTGTTCTATATTTAGGATTATCTGAAATTTGTTCCGCAATAATATCTTGTGCACCTTTGATAGCATCTTCAACCGATTGCACTTCTTCATTTATAAATTGTTGTGCTTTTTCTTCAATTGAGACTTCATGTTTACGTG  >  CP000730/2184293‑2184565                                                                                                                                          |                                                                                                                                        aaaCTCTATGATTAGCAATGCGTTTAATTGGCTCACTATATGCATAGTACATTTCAAAAATACCTTTTTCATCTTCAGCATTTTTCTTTTTAGATGTAGTTAACACACCTTGATGATACATATCTTTTAAAATTTTTTTTc                                                                                                                                      <  2:115644/141‑1 (MQ=255)                     cGTTTAATTGGCTCACTATATGCATAGTACATTTCAAAAATACCTTTTTCATCTTCAGCATTTTTCTTTTTAGATGTAGTTAACACACCTTGATGATACATATCTTTTAAAATTTTTTTTCTATATTTAGGATTATCTGaa                                                                                                                  <  2:19818/141‑1 (MQ=255)                                                  cATTTCAAAAATACCTTTTTCATCTTCAGCATTTTTCTTTTTAGATGTAGTTAACACACCTTGATGATACATATCTTTTAAAATTTTTTTTCTATATTTAGGATTATCTGAAATTTGTTCCGCAATACTATCTTGTGCAcc                                                                                     >  2:38672/1‑141 (MQ=255)                                                   aTTTCACAAATACCTTTTTCATCTTCAGCATTTTTCTTTTTAGATGTAGTTAACACACCTTGATGATACATCTCTTTTAAAATTTTTTTTCTATATTTAGGATTATCTGAAATTTGTTCCGTAATAATAtttttttctcct                                                                                    >  2:113744/1‑133 (MQ=255)                                                    tttCAAAAATACCTTTTTCATCTTCAGCATTTTTCTTTTTAGATGTAGTTAACACACCTTGATGATACATATCTTTTAAAATTTTTTTTCTATATTTAGGATTATCTGAAATTTGTTCCGTAATTATTTCTTGTGTACttt                                                                                   >  2:58771/1‑141 (MQ=255)                                                            aTACCTTTTTCATCTTCCGCATTTTTCTTTTTAGATGTAGTTAACACACCTTGATGATACATATCTTTTAAAATTTTTTTTCTATATTTAGGATTATCTGAAATTTGTTCCGCAATAATATCTTGTGCACCTTTGATAGCa                                                                           <  2:146044/141‑1 (MQ=255)                                                                                    ttCTTTTTAGATGTAGTTAACACACCTTGATGATACATATCTTTTAAAATTTTTTTTCTATATTTAGGATTATCTGAAATTTGTTCCGCAATAATATCTTGTGCACCTTTGATAGCATCTTCAACCGATTGCACTTCTTCa                                                   >  2:464429/1‑141 (MQ=255)                                                                                              aTGTAGTTAACACACCTTGATGATACATATCTTTTAAAATTTTTTTTCTATATTTAGGATTATCTGAAATTTGTTCCGCAATAATATCTTGTGCACCTTTGATAGCATCTTCAACCGATTGCACTTCTTCATTTATAAAtt                                         <  2:210399/141‑1 (MQ=255)                                                                                               tGTAGTTAACACACCTTGATGATACATATCTTTTAAAATTTTTTTTCTATATTTAGGATTATCTGAAATTTGTTCCGCAATAATATCTTGTGCACCTTTGATAGCATCTTCAACCGATTGCACTTCTTCATTTATAAAttg                                        >  2:234902/1‑141 (MQ=255)                                                                                                gTAGTTAACACACCTTGATGATACATATCTTTTAAAATTTTTTTTCTATATTTAGGATTATCTGAAATTTGTTCCGCAATAATATCTTGTGCACCTTTGATAGCATCTTCAACCGATTGCACTTCTTCATTTATAATttgt                                       >  2:60650/1‑141 (MQ=255)                                                                                                        cacacCTTGATGATACATATCTTTTAAAATTTTTTTTCTATATTTAGGATTATCTGAAGTTTGTTCCGCAATAATATCTTGTGCACCTTTGGTAGCATCTTCAACCCATTGCACTTCTTCCCTTATAAATTGTTGTTCttt                               >  2:52631/1‑141 (MQ=255)                                                                                                             cTTGATGATACATATCTTTTAAAATTTTTTTTTTTTTTTTAGGATTATCTGAAATTTGTTCCGCAATCATATCTTGTGCACCTTTGATAGCATCTTCAACCGATTGCACTTCTTCATTTATACATTGTTGTGCTTTttctt                          >  2:59129/1‑141 (MQ=255)                                                                                                                      atatatCTTTTAAAATTTTTTTTCTATATTTAGGATTATCTGAAATTTGTTCCGCAATAATATCTTGTGCACCTTTGATAGCATCTTCAACCGATTGCACTTCTTCATTTATAAATTGTTGTGCTTTTTCTTCAATTgaga                 <  2:415650/139‑1 (MQ=255)                                                                                                                          atCTTTTAAAATTTTTTTTCTATATTTAGGCTTATCTGAAATTTGTTCCGCAAAAATATCATGAACCCCATTGATATCACGTTCAACCGACTGCACATCATCATTCATAAAGTGTTGTTCTTTTTCCTCAATTGAGACTTc             >  2:342468/1‑141 (MQ=255)                                                                                                                             ttttAAAATTTTTTTTCTATATTTAGGATTATCTGAAATTTGTTCCGCAATAATATCTTGTGCACCTTTGATAGCATCTTCAACCGATTGCACTTCTTCATTTATAAATTGTTGTGCTTTTTCTTCAATTGAGACTTCATg          <  2:300689/141‑1 (MQ=255)                                                                                                                                 aaaaTTTTTTTTCTATATTTAGGATTATCTGAAATTTGTTCCGCAATAATATCTTGTGCACCTTTGATAGCATCTTCAACCGATTGCACTTCTTCATTTATAAATTGTTGTGCTTTTTCTTCAATTGAGACTTCATGTTTa      <  2:496217/141‑1 (MQ=255)                                                                                                                                     ttttttttCTATATTTAGGATTATCTGAAATTTGTTCCGCAATAATATCTTGTGCACCTTTGATAGCATCTTCAACCGCTTGCACTTCTTCATTTATAAATTGTTGTGCTTTTTCTTCAATTGAGACTTCATGTTTACGTg  <  2:226469/141‑1 (MQ=255)                                                                                                                                     ttttttttCTATATTTAGGATTATCTGAAATTTGTTCCGCAATAATATCTTGTGCACCTTTGATAGCATCTTCAACCGATTGCACTTCTTCATTTATAAATTGTTGTGCTTTTTCTTCAATTGAGACTTCATGTTTACGTg  <  2:368564/141‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        AAACTCTATGATTAGCAATGCGTTTAATTGGCTCACTATATGCATAGTACATTTCAAAAATACCTTTTTCATCTTCAGCATTTTTCTTTTTAGATGTAGTTAACACACCTTGATGATACATATCTTTTAAAATTTTTGTTCTATATTTAGGATTATCTGAAATTTGTTCCGCAATAATATCTTGTGCACCTTTGATAGCATCTTCAACCGATTGCACTTCTTCATTTATAAATTGTTGTGCTTTTTCTTCAATTGAGACTTCATGTTTACGTG  >  CP000730/2184293‑2184565

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑