Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	134,393	G→A	L12L (TTG→TTA)	sbnA →	possible pyridoxal‑phosphate dependent enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	134,393	0	G	A	100.0%	56.6 / NA	20	L12L (TTG→TTA)	sbnA	possible pyridoxal‑phosphate dependent enzyme
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/8);  total (12/8)

TTACTTGATAGTGAGAATCATTATCAATTAGGTAACACACAATATTATAGAATTTTAAATTTGAGGAGGAAGCGCTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTGTTAGATTCTGTAGGGCAAACACCTATGGTTCAACTTCATCAACTATTTCCGAAACATGAAGTGTTTGCAAAGTTAGAGTATATGAATCCTGGAGGCAGCATGAAAGATCGACCTGCCAAG  >  CP000730/134281‑134513                                                                                                                 |                                                                                                                         ttACTTGATAGTGAGAATCATTATCAATTAGGTAACACACAATATTATAGAATTTTAAATTTGAGGAGGAAGCGCTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATgg                                                                                              >  2:182774/1‑141 (MQ=255)     ttGATAGTGAGAATCATTATCAATTAGGTAACACACAATATTATAGAATTTTAAATTTGAGGAGGAAGCGCTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCa                                                                                          >  2:256246/1‑141 (MQ=255)               aaaacATTACCAATTAAGTAACACACAAAAAGATAGAATTTGAAATCTGAGGAGGAAGCTAAAAAAAAAGAAAAAAGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACTTCATCaa                                                                                <  2:481444/137‑1 (MQ=255)                   cATTATCAATTAGGTAACACACAATATTATAGAATTTTAAATTTGAGGAGGAAGCGCTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACTTCATCGACTAt                                                                            >  2:361339/1‑141 (MQ=255)                        tcagatAGGTAACACACAATATTATAGAATTTTAAATTTGAGGAGGCAGCCCTTTTGATTGAATAAAGTCAAGCATTGCACGATTAATTATTAGATCCTGTAGGGCAAACACCTATGGGTCAACTTCATCAACTATTTCCg                                                                       <  2:73022/136‑1 (MQ=255)                             tAGGTAACACACAATATTATAGAATTTTAAATTTGAGGAGGAAGCGCTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACTTCATCAACTATCTCCGAAACa                                                                  >  2:467576/1‑141 (MQ=255)                                 tAACACACAATATTATAGAATTTTAAATTTGAGGAGGAAGCTCTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTATTATATTCTGTAGGGCAAACACCTATGGTTCAACTTCATCAACTATTTCCGAAACATGaa                                                              <  2:469787/141‑1 (MQ=255)                                     acacaATATTATAGAATTTTAAATTTGAGGAGGAAGCGCTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACTTCATCAACTATTTCCGAAACATGAAgtgt                                                          <  2:119082/141‑1 (MQ=255)                                         aatttaataGAAATTTAAATCTGAGGAGGAAGCGCTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACTTCATCAACTATTTCCGAAACATGAAGTGTTTGc                                                      <  2:215943/135‑1 (MQ=255)                                            attatAGAATTTGAAATTTGAGGAGGAAGCGCTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACTGCATCAACTATTTCCGAAACATGAAGTGTTTGCaaa                                                   <  2:374029/141‑1 (MQ=255)                                                         aaaTTTGAGGAGGCAGCGCTATTGATTGAAAAAAGTCAAGCACGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACTTCATCAACTATTTCCGAAACATGAAGTGTTTGCAAAGTTAGAGTATATg                                      >  2:470915/1‑141 (MQ=255)                                                         aaaTTTGAGGAGGAAGCGCTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACTTCATCAACTATTTCCGAAACATGAAGTGTTTGCAAAGTTAGAGTATATg                                      >  2:150128/1‑141 (MQ=255)                                                         aaaTTTGAGGAGGAAGCGCTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACTTCATCAACTATTTCCGAAACATGAAGTGTTTGCAAAGTTAGAGTATATg                                      >  2:156400/1‑141 (MQ=255)                                                           aTTTGAGGAGGAAGCGCTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCGACTTCATCAACTATTTCCGAAACATGAAGTGTTTGCAAGGTTAGAGAATATGaa                                    >  2:199015/1‑141 (MQ=255)                                                           aTTTGAGGAGGAAGCGCTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACTTCATCAACTATTTCCGAAACATGAAGTGTTTGCAAAGTTAGAGTATATGaa                                    >  2:401944/1‑141 (MQ=255)                                                                  gaggaAGCGCTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACTTCATCAACTATTTCCGAAACATGAAGTGTTTGCAAAGTTAGAGAATATGAATCCTGGa                             >  2:409050/1‑141 (MQ=255)                                                                                 attgaAAAAAGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACTTCATCAACTATTTCCGAAACATGAAGTGTTTGCAAAGTTAGAGTATATGAATCCTGGAGGCAGCATGAAAGAt              >  2:495728/1‑141 (MQ=255)                                                                                 attgaAAAAAGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACTTCATCAACTATTTCCGAAACATGAAGTGTTTGCAAAGTTAGAGTATATGAATCCTGGAGGCAGCATGAAAGAt              <  2:6049/141‑1 (MQ=255)                                                                                      aaaaaGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACTTCATCAACTATTTCCGAAACATGAAGTGTTTGCAAAGTTAGAGTATATGAATCCTGGAGGCAGCATGAAAGATCGAcc         <  2:486301/141‑1 (MQ=255)                                                                                             cAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACTTCATCAACTATTTCCGAAACATGAAGTGTTTGCAAAGTTAGAGTATATGAATCCTGGAGGCAGCATGAAAGATCGACCTGCCAAg  >  2:191848/1‑141 (MQ=255)                                                                                                                 |                                                                                                                         TTACTTGATAGTGAGAATCATTATCAATTAGGTAACACACAATATTATAGAATTTTAAATTTGAGGAGGAAGCGCTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTGTTAGATTCTGTAGGGCAAACACCTATGGTTCAACTTCATCAACTATTTCCGAAACATGAAGTGTTTGCAAAGTTAGAGTATATGAATCCTGGAGGCAGCATGAAAGATCGACCTGCCAAG  >  CP000730/134281‑134513

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑