Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	396,540	G→A	A208T (GCA→ACA)	USA300HOU_0364 →	possible lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	396,540	0	G	A	100.0%	79.8 / NA	30	A208T (GCA→ACA)	USA300HOU_0364	possible lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/13);  total (17/13)

ATACCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAACGGCGGTTATGAGTC  >  CP000730/396414‑396676                                                                                                                               |                                                                                                                                         aTACCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGAGTTATATGATTTTAAAGCGAAAGTTGAAGGCACACCAAAAATTTAt                                                                                                                            >  2:432057/1‑141 (MQ=255)    ccAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGAc                                                                                                                         <  2:14240/141‑1 (MQ=255)     cAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAACATCACAGGGGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAACACACCGTTGAAGGCACACAAAACATTTATGCCt                                                                                                                        >  2:210388/1‑141 (MQ=255)     aaaaaTTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAAAAAAACAAAAAATTTATGACt                                                                                                                        <  2:256482/140‑1 (MQ=255)       aaaTTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCAAAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAAATTATGACTTa                                                                                                                      >  2:487070/1‑141 (MQ=255)        aaTTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTAt                                                                                                                     <  2:314138/141‑1 (MQ=255)             aTGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTaaa                                                                                                                >  2:37151/1‑141 (MQ=255)                  aCAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTAt                                                                                                           >  2:262083/1‑141 (MQ=255)                      ggTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATATAAACCTATTTTa                                                                                                       >  2:72597/1‑141 (MQ=255)                      ggTTCAGTTGACCTATTAAATGAAGTAGCAACTTCTAAAATCACAGGTGAAGAAAAAAATAAAAAAAATACAGATATATACGATTTTAAAGCGAACGTTAAAGGCAGACAAAAAATTTATGACTTATTTaaaaaaaaaaaa                                                                                                       <  2:472804/141‑10 (MQ=255)                         tCTGTTGACCTATTAAATGAAGTTGCAACGTCTAAAACCACAGGTGAAGAAGAAATTGATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAACCGTGCATGACTTATTTTTACCTATTTTagag                                                                                                    <  2:456197/141‑1 (MQ=255)                               gACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGaaaaaa                                                                                              >  2:406480/1‑141 (MQ=255)                               gACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGAATTATTTAAACCTATTTTAGAGaaaaaa                                                                                              >  2:451287/1‑141 (MQ=255)                                 ccTATTAAATGAAGTTGCAACTTCTAAAATCACAGGGGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGa                                                                                            <  2:178859/141‑1 (MQ=255)                                         ttGAAGTTGCAACTGCTAACAGCACAGGTGAAAAAGAAAGTGATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTACACCTATTTTAGAGAAAAAAGATAAAAAAt                                                                                    <  2:48789/140‑1 (MQ=255)                                            aaGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTaa                                                                                 >  2:452531/1‑141 (MQ=255)                                                    aCTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGtgatgaa                                                                         >  2:484406/1‑140 (MQ=255)                                                    aCTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTgatgat                                                                         >  2:198775/1‑141 (MQ=255)                                                    aCTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATCTATATGATGTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTgatgat                                                                         >  2:478538/1‑141 (MQ=255)                                                     cTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAAGTAAGTGATGata                                                                        >  2:63618/1‑141 (MQ=255)                                                                acaGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAAc                                                             <  2:10717/141‑1 (MQ=255)                                                                    gTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCg                                                         >  2:20248/1‑141 (MQ=255)                                                                                tttATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATc                                             <  2:364905/141‑1 (MQ=255)                                                                                          tACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGa                                   >  2:436782/1‑141 (MQ=255)                                                                                              aaTTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATaaa                               <  2:238818/140‑1 (MQ=255)                                                                                                         ttttAAAGCGAACGTTGAAAGCACAACAAAAAGTTATGACTTATTTAAACCTATTTTAGAGAAAAGAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGACTCAATCATTGTAttgttattaattcaa                    <  2:475990/141‑15 (MQ=255)                                                                                                          tttAAAGCGAACGTTGAAGGCACACCAAAAAATTACAACCTATTTAAACCTAAATTAGACAAAAAATATAAAAAATTAAGTGAAGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATaac                   <  2:166190/141‑1 (MQ=255)                                                                                                          tttAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATaac                   <  2:39135/141‑1 (MQ=255)                                                                                                                 cGAACGTTGAAGGCACACAAAAAATTTATGAATTATTTAAACCTATTATAGAGAGAAGAGATAACAACTTAAGTGATGATATCCAAATGAACTTCGATAAAGGGAATCAATTATTGGATAAATATAAAGATAACAacgacg            >  2:190190/1‑141 (MQ=255)                                                                                                                           aGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAACGGCGGTTATGAGTc  >  2:357335/1‑141 (MQ=255)                                                                                                                               |                                                                                                                                         ATACCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAACGGCGGTTATGAGTC  >  CP000730/396414‑396676

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑