| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 804,673 | A→T | 100% | L135I (TTA→ATA) | dtpT ← | POT family proton (H+)‑dependent oligopeptide transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 804,673 | 0 | A | T | 100.0% | 96.0 / ‑6.5 | 34 | L135I (TTA→ATA) | dtpT | POT family proton (H+)‑dependent oligopeptide transporter |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base T (17/16); minor base G (0/1); total (17/17) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AAGTGTTGCAAAATAATAGGTGATAATAATGCACCCATATTAACTGACATATAGAAAATAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCACCTAATAAAGTAGCGCCTCTAGTGCCTGTAATT > CP000730/804537‑804803 | aaGTGTTGCAAAATAATAGGTGATAATAACGCACCCATATTAACTAACATATAGAAAATAACAAAACCCGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTATAcc < 2:373007/140‑1 (MQ=255) ttttGCAAAATAATAGGTGATAATAATGCACCCATATTAACTGACATATAGAAAATAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACAATATCGCAAAGGGTTGGCTTCATTATACCTGa < 1:404764/138‑1 (MQ=255) taataGGTGATAATAATGCACCCATATTAACTGACATATAGAAAATAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAGACCTGAACCAATAatg < 1:307281/140‑1 (MQ=255) ataGGTGATAATAATGCACCCATATTAACTGACATATAGAAAATAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTATACCTGAACCAATAatgat > 2:533935/1‑140 (MQ=255) aGGTGATAATAATGCACCCATATTAACTGACATATAGAAAATAACAAAACCTGCATCCATACGTCTATCATTTTCAGAATATAAACGGCCAACGATAATAAAAAAGTTTGGCTAAAATATAACGCAACCAACAAtgatga > 1:118300/1‑140 (MQ=255) aGGTGATAATAATGCACCCATATTAACTGACATATAGAAAATAACAAAACCTGCAACCATACGTCTATCATTTTCAGGATATAAACGGCCAACAATAATTGAAATGTTAGACCACAATATACCTGCACCaataatcctga > 1:462314/1‑135 (MQ=255) gagATAATAATACACCCATATTAACTAACATATAAAAAATAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATAAAAACGGCCAACGATATTAGAAATGATTGGCTTCATTATACCTGAACCAATAATGATgaag < 1:75918/138‑1 (MQ=255) tataataATGCACCCATATTAACTGACATATAGAAAATAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTATACCTGAACCAATAATGATgaagaa < 1:312111/139‑1 (MQ=255) gATAATAATGCACCCATATTAACTGACATATAGAAAATAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTATACCTGAACCAATAATGATgaagaa < 1:454008/140‑1 (MQ=255) ataatGCACCCATATTAACTGACATATAGAAAATAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTATACCTGAACCAATAATGATGAAGAACAtt < 1:473856/140‑1 (MQ=255) aatGCACCCATATTAACTGACATATAGAAAATAACAAAACTTGCATCCATACGTCTATCAGTTTCAGGATATAAACGGCCAACGAGATTTGAAATGTTTGGCTTCATTATATCTGAACCCATAATGATGAAGAACATTGa < 1:356214/140‑1 (MQ=255) aatGCACCCATATTAACTGACATATAGAAAATAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTATACCTGAACCACTAATGATgaagaaaacccc > 2:548269/1‑134 (MQ=255) ctGCACCCATATTAACTGACATATAGAAAATAACAAAACGAGCATCCTTACGTCTATCATTTGCAGGATATAAACGGCCAACGAGATTTGAATTGTTTGGGTTCTTTATACCTGAACCAATAATGATGAAGAACATTGAt > 2:353165/2‑140 (MQ=255) tGCACCCATATTAACTGACATATAGAAAATAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTATACCTGAACCAATAATGATGAAGAACATTGAtg > 2:192486/1‑140 (MQ=255) gtAACTGACCTATAGAAAATAACAAAGCCTGCATCCAGACGTGTATCATGTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTATACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGcc < 2:484144/139‑1 (MQ=255) cTGACATATAGAAAATAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTATACCTGAACCAATAATGATGAAGCACATTGATGTGAATAAGCCGAtt > 2:344213/1‑140 (MQ=255) cTGACATATAGAAAATAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGAAAGGCTTCATAATACCTGAAAAAATAAAGATAAAAAACAAAGACATGAATAAGCCGaaa > 1:410414/1‑138 (MQ=255) atatAGAAAATAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTATACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCCGTTAATGc > 2:259946/1‑140 (MQ=255) aGAAAATAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTATACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCaaaa > 2:479965/1‑139 (MQ=255) aGAAAATAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTATACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAAt < 1:501044/140‑1 (MQ=255) aaaaTAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTATACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATgg < 1:287645/140‑1 (MQ=255) aaaTAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCTACGATATTTGAAATGTTTGGCTTCATTATACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCCAATGGt < 2:304009/140‑1 (MQ=255) aaCAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTATACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGc > 2:53239/1‑140 (MQ=255) aCAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCTAACGATATTTGAAATGTTTGGCTTCATTATACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCt < 1:76479/140‑1 (MQ=255) cAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTATACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAAGGCAAATGGTAAGCtt > 2:203481/1‑140 (MQ=255) aaaCCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTATACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTaa > 2:297374/1‑140 (MQ=255) tATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTATACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGctaata > 2:19130/1‑140 (MQ=255) aTCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTATACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGataataa < 1:430780/140‑1 (MQ=255) ttCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTATACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGa < 1:192486/140‑1 (MQ=255) gggtgcaaaCGGCCATCGATATTTGAAGTGTTTGGCTTCATTATACCGGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACGAATATGTCCGATAATAATAAAGACTGc < 2:133701/134‑1 (MQ=255) aaCGATATTTGAAATGTTTGGCTTCATTATACCTGAACCAATAATGAGGGGGGACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCACCTAATAAAGTAg > 2:500655/1‑140 (MQ=255) gATATTTGAAATGTTTGGCTTCATTATACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCACCTAATAAAGTAgcgc > 2:463254/1‑140 (MQ=255) gCTTCATTATACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCCCCTAATAAAGTAGCGCCTCTAGTGCCTGTAAtc > 2:479736/1‑139 (MQ=255) gCTTCATTATACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCACCTAATAAAGTAGCGCCTCTAGTGCCTGTAAtt < 2:462314/140‑1 (MQ=255) | AAGTGTTGCAAAATAATAGGTGATAATAATGCACCCATATTAACTGACATATAGAAAATAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCACCTAATAAAGTAGCGCCTCTAGTGCCTGTAATT > CP000730/804537‑804803 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |