Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,416,458 A→T 100% I216N (ATT→AAT)  oppD ← oligopeptide ABC superfamily ATP binding cassette transporter, ABC protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,416,4580AT98.1% 128.7 / ‑5.4 44I216N (ATT→AAT) oppDoligopeptide ABC superfamily ATP binding cassette transporter, ABC protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (21/22);  minor base G (1/0);  total (22/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ACTCAATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAAATGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACTG  >  CP000730/1416326‑1416590
                                                                                                                                    |                                                                                                                                    
aCTCAATGGTAGTAAGCCCTCTTTATTTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTg                                                                                                                               <  2:83077/140‑1 (MQ=255)
 cTCAATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGCCGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCGCGTTCAATGATTTGACATTCTTTTAAAACATAATTGTACTGa                                                                                                                              >  2:312539/1‑140 (MQ=255)
      tCATAGTAATCCCTTTTTAATTGTGTTCTATATTTAATTTGGCGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAata                                                                                                                         <  1:110742/140‑1 (MQ=255)
      tCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAata                                                                                                                         <  1:110707/140‑1 (MQ=255)
      tCATAGGAATCCCTCGTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAata                                                                                                                         <  2:213958/140‑1 (MQ=255)
       cATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATTCTCATACTTGAAATGATTAATATTACCTCATTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAatat                                                                                                                        >  1:478867/1‑140 (MQ=255)
        ttattattCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATAtt                                                                                                                       <  2:427887/133‑1 (MQ=255)
           gTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATAATTAATATTACCTAGTTCAATGATTTGACCTTCTTTTAAAACATATTTATACTGTAAATATTTCa                                                                                                                    >  1:288157/1‑140 (MQ=255)
           ctAATCCCTCTTTTATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTTAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCa                                                                                                                    <  2:80966/139‑1 (MQ=255)
                    ctTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGAc                                                                                                           >  2:4268/1‑140 (MQ=255)
                       tAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTa                                                                                                        >  1:288674/1‑140 (MQ=255)
                          ttGTGTTCTATATTTAATTAGACGTTCAGTAAACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGt                                                                                                     <  1:414203/140‑1 (MQ=255)
                          tgttgtTCTTTATTTAATTAGCCGTTCAGCTTACGGATGAAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGt                                                                                                     <  1:41552/137‑1 (MQ=255)
                                     atTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACAAAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGata                                                                                          >  1:420056/1‑140 (MQ=255)
                                         aaTTAGTGGTTCAGTATACGCATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGAtaataa                                                                                      <  2:316352/140‑1 (MQ=255)
                                               aCGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCTTTCAATGATTTGACCTTCTTTTAAAAAATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATg                                                                                >  1:193310/1‑140 (MQ=255)
                                                       tataCGGATGCAAATGCTCATACTTGAAATGATTAATCTTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGTCATTCTTTCAATACATTACTTAAGTTATGTATGATAATAAATAATGTTTGACCa                                                                        >  1:108087/1‑140 (MQ=255)
                                                       tataCGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTTACCTTCTTTTAAAACATAATTGTAATGAAAATATAGAAATACATGACTTACGTTATGTGTGATAATAAATAATGTTTTACCa                                                                        >  1:209302/1‑140 (MQ=255)
                                                       tataCGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATAACTTAAGTAATGTGTGATAATAAATAATGTTTGACCa                                                                        >  2:130292/1‑140 (MQ=255)
                                                         taCGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTTAGCGATCTAATTAATAATGTTTGACCATg                                                                      <  2:118387/140‑1 (MQ=255)
                                                         aactgttGCAAATGCTCATACTTGAAATCATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATg                                                                      <  2:408874/134‑1 (MQ=255)
                                                           cGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGtt                                                                    >  2:513910/1‑140 (MQ=255)
                                                            ggATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGCCTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTc                                                                   <  2:413907/140‑1 (MQ=255)
                                                                  aaaTGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATAc                                                             <  1:479068/140‑1 (MQ=255)
                                                                    aTGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACaa                                                           >  1:60023/1‑140 (MQ=255)
                                                                         cATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGc                                                      <  2:288927/140‑1 (MQ=255)
                                                                          aTACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTGCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTAACCATGTTCTAATACataatgat                                                     >  2:414287/1‑133 (MQ=255)
                                                                            aCTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCCATACATGCCTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAAAATGCTGt                                                   >  1:250749/1‑140 (MQ=255)
                                                                                   aTGATTAATAGTACCTCGTTCCTTGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAAt                                            <  2:98726/140‑1 (MQ=255)
                                                                                     gATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATcc                                          <  1:253006/140‑1 (MQ=255)
                                                                                      aTTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCa                                         <  1:169817/140‑1 (MQ=255)
                                                                                      aTTAACATTACTGCGTTAAATTATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCa                                         <  1:535532/140‑1 (MQ=255)
                                                                                                ccTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTTTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGAtt                               <  1:55809/140‑1 (MQ=255)
                                                                                                 cTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTa                              >  1:462977/1‑140 (MQ=255)
                                                                                                  tCGTTCAATGATTTGACCTTCTTTTACAACTTAAGTTTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTAc                             >  1:266690/1‑140 (MQ=255)
                                                                                                    gTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATATGAGATAATAAATAATGTTTGAACATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCg                           >  1:59156/1‑140 (MQ=255)
                                                                                                     ttCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTCATAAATCCATCACTTGATTACCGt                          >  2:300109/1‑140 (MQ=255)
                                                                                                     tgtgacgATTTGACCTTGTTGTAAAACATAATTGTCCTGACAATATTTCAATACATGATTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATCAATCCATCACTTGATTACCGt                          <  2:32198/134‑1 (MQ=255)
                                                                                                      tCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGCCTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTCATTACCGtt                         >  2:331874/1‑140 (MQ=255)
                                                                                                        aaTGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCa                       >  1:499900/1‑140 (MQ=255)
                                                                                                                        tttAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGc       <  2:60753/140‑1 (MQ=255)
                                                                                                                          tAAAACAGAATGGTACTGACAATATTTCAATCTATGACTTAAGTTATGTGTGAGAATAAATAATGTTTGGCCATGTTCTAATACAATATGCAGTAATAAATCCATCACTTGATTACCGTACAAAGCATCTAATGATTCaa     >  2:459923/1‑140 (MQ=255)
                                                                                                                            aaaCATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACt   >  2:403722/1‑140 (MQ=255)
                                                                                                                             aaCATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATTCAATGATGCAACTg  <  1:359488/140‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                    
ACTCAATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAAATGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACTG  >  CP000730/1416326‑1416590

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: