Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,010,363 C→T 100% C146Y (TGT→TAT)  ygaD ← ABC superfamily ATP binding cassette transporter, membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,010,3630CT100.0% 94.3 / NA 35C146Y (TGT→TAT) ygaDABC superfamily ATP binding cassette transporter, membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/16);  total (19/16)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTTCACGTGTCAATTTTCTTAATCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATACAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCTTGTAAATGGTTG  >  CP000730/2010226‑2010497
                                                                                                                                         |                                                                                                                                      
tttCACGTGTCAATTTTCTTAATCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATaa                                                                                                                                      >  2:347382/1‑140 (MQ=255)
 ttCACGTTGCAATTTTCTTAATCTTCCAAAGAAAACGTACACCGCTAAAATGTAAAATGGGAAGATAAAAACTGACGCTGAAGTGAATTTCACATCTAAAAAGAACCTTATGGATAGTGCAATAATAATTGTTATATAAt                                                                                                                                     <  2:87891/140‑1 (MQ=255)
 ttCACGTCTCAATTTTCTTAATCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAAt                                                                                                                                     <  2:159892/140‑1 (MQ=255)
   cACGTGTCAATTTTCTTAATCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCt                                                                                                                                   >  1:32037/1‑140 (MQ=255)
           aaTTTTCTTAATCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATCTAATCTAACCAAat                                                                                                                           >  2:278113/1‑140 (MQ=255)
                    aaTCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTATAGTCAATTTCACATCTAAAAAGAATTGTCTGCAAAGGACAATAATAATTGTTATATAATCTAACCAAAAATTCATTaa                                                                                                                  >  1:260109/1‑140 (MQ=255)
                     aTCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTaaa                                                                                                                 >  2:324494/1‑139 (MQ=255)
                     aTCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAAc                                                                                                                 >  2:51947/1‑140 (MQ=255)
                       catCCAAAGAAAACGTACACCGTTAAAATGTTAAATGGGAAGATAAACAGTGTTGCGAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAAccc                                                                                                               <  2:537030/138‑1 (MQ=255)
                       cTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAAATAGACCGTGCTGCGCAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCTATAATACTTGTTAGATAATCTACCAAAATATACATCAAacc                                                                                                               >  1:510439/1‑140 (MQ=255)
                               gAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTaaaa                                                                                                       >  1:522800/1‑140 (MQ=255)
                                   aCGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGaa                                                                                                   >  1:274023/1‑140 (MQ=255)
                                   aCGTACACAGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAATCAAATATTCATGAAACCGGTCAAAATGaa                                                                                                   >  2:237849/1‑140 (MQ=255)
                                         acCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCttt                                                                                             <  2:391821/140‑1 (MQ=255)
                                                  atgtaaaatgTGAAGATAAACATTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATGATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTc                                                                                    <  2:467408/140‑1 (MQ=255)
                                                    gtaaaatgGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCaa                                                                                  >  1:520997/1‑140 (MQ=255)
                                                      aaaatgGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAAca                                                                                >  2:189043/1‑140 (MQ=255)
                                                          tgGGAAGATAAAGAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTCACCCGGTTAAAATGAAATCTTTTGTTTGGTCAAcatcat                                                                            >  1:389420/1‑140 (MQ=255)
                                                          tgGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATTGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCGGGTTAAAATGAAATCTTTTTTTGGTTCAAcatcat                                                                            <  2:172580/140‑1 (MQ=255)
                                                          tgGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAAcatcat                                                                            >  1:1219/1‑140 (MQ=255)
                                                             gAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGATTAAAATGAAATCTTTTGTTTGTTCAACATCATTaa                                                                         >  1:91718/1‑140 (MQ=255)
                                                                  tAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCAct                                                                    >  2:460477/1‑140 (MQ=255)
                                                                           ctgctAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATAtt                                                           <  1:415265/140‑1 (MQ=255)
                                                                                  aatcactatCACATCCAAAAAAAACACTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGAc                                                    <  2:106764/132‑1 (MQ=255)
                                                                                                              aTGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTGATTTGATCAACATCATTAATCACACTAGATATTACTTGACCTACTTTATTATTAGCATAAAATCACGc                        >  1:448747/1‑140 (MQ=255)
                                                                                                                 gATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCATTCTAGATATGACTTGACCTACTTGATTATTAGCATAAAATCTCGCACt                     <  1:289967/140‑1 (MQ=255)
                                                                                                                 gATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACt                     <  2:524210/140‑1 (MQ=255)
                                                                                                                           taataatTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTTTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCTTGt           <  1:55221/140‑1 (MQ=255)
                                                                                                                            aataatTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCTTGTa          <  2:520997/140‑1 (MQ=255)
                                                                                                                             ataatTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCTTGTaa         <  1:97800/140‑1 (MQ=255)
                                                                                                                              taatTGTTATATAATCTAACCACATATTCATTAGCACGGTTAAACTGACATTTTTTGTTTGTTCAACATCATTAATAACTCTCGATATTACTTGAGCAACTTGCATTTTCGCATAAAATCTGGCAATTAATGCTTGTaaa        >  2:178266/1‑140 (MQ=255)
                                                                                                                               aatTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGAACTTAAAGCTTGTAAAt       >  1:307782/1‑140 (MQ=255)
                                                                                                                                  tGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCAGAAAATCTCGCACTTAAAGCTTGTAAATGGt    <  1:76826/140‑1 (MQ=255)
                                                                                                                                  tGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACAAGATTACTAGCATAAAATCTCGCACTTAAAGCTTGTAAATGGt    <  2:501570/140‑1 (MQ=255)
                                                                                                                                    ttATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTTAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCTTGTAAATGGTTg  <  1:209674/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                      
TTTCACGTGTCAATTTTCTTAATCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATACAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCTTGTAAATGGTTG  >  CP000730/2010226‑2010497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑