| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 2,532,298 | A→T | 100% | T15T (ACA→ACT) | murN → | FemAB family peptidoglycan biosynthesis protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 2,532,298 | 0 | A | T | 97.7% | 121.8 / ‑4.8 | 41 | T15T (ACA→ACT) | murN | FemAB family peptidoglycan biosynthesis protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base T (13/27); minor base C (0/1); total (13/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GTATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACAACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAGCAC > CP000730/2532164‑2532434 | gtATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACaaa < 2:392048/140‑1 (MQ=255) tATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAg < 1:391231/140‑1 (MQ=255) agtggTAATCAGGTGTGGAAAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATGATAGTTCAATTCAGAGAGGGATGGGATAAAAGGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGc < 1:501237/136‑1 (MQ=255) aTTTGTAATCAGTTTTTGATAAAATAAAAAAATATCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTGAATGCGTTTACTACAAAGc < 2:155642/140‑1 (MQ=255) aTTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGc < 2:519196/140‑1 (MQ=255) aTTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGc < 2:467883/140‑1 (MQ=255) tttGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCa < 2:170065/140‑1 (MQ=255) tGTAATTAGTTTTTGATAAAATAAAAAAATAGCATTATTAATTAATCCAAAAATGATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTGTGTACCGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCAtt < 2:12947/140‑1 (MQ=255) gTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGATGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCAttt < 1:37372/140‑1 (MQ=255) gTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCAttt < 1:396033/140‑1 (MQ=255) aTCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTCCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTc > 2:238511/1‑140 (MQ=255) tttttGATAAAATAAAAAAATAGCCTTATTAATAACTTCAAAACTTATTGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCATAtt < 2:2166/140‑1 (MQ=255) aaaaaataaaaAAATACGATTATTAATAAATTCAAAAATTATAGTTAAATCCAGAGAGGTATGTGAAAAAATGAACTTTGCAACGTTGACTTCGGATGAGTTCAATGCGCTGACCACAACGCACTTTTCACATTacacac < 2:142458/138‑1 (MQ=255) aaaataaaaAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACaa < 1:270677/140‑1 (MQ=255) aaaaaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCt < 1:428637/140‑1 (MQ=255) aaaaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAAACAGCTa > 1:94981/1‑140 (MQ=255) tattaATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTTTAACGTTGACTTCGGATGAGTTCAATGCGTTTTCTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAAt < 1:171268/140‑1 (MQ=255) taataaGTTCAAAAATTATAGTTCAATTCAGGGAGGGATGGGATAAAATGAACTTTGTAACGTTTACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTGTTCACATTACACACAATCAGCTATTCATTACAATCAt < 2:247978/140‑1 (MQ=255) taataaATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCAt < 2:304590/140‑1 (MQ=255) aaaaaTTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGAtt < 2:256746/140‑1 (MQ=255) aTTATAGTTCAATTCAAAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCTCGTCACGCACAATCAGCTATTCATTACAATCATAGAGTTGATTTaaa < 1:378425/140‑1 (MQ=255) aGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATTAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCg < 1:281220/140‑1 (MQ=255) aGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGAATTAAAAAGCg > 1:156490/1‑140 (MQ=255) aGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTAAATGCGTTTACTACAAAGCATTTTTTAAATTACACAGAATCAGCTATTCATTACAATCATAGAGTTGATTTaaaaagag > 1:509639/1‑136 (MQ=255) tCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGTTATTCATTACAATCATAGATTTGATTTAAAAGGCGAtg < 2:254506/140‑1 (MQ=255) ggatgggatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTCCAATCATAGAATAAATTTAAAATGCGATGTGCATCtgttaga > 1:258715/1‑134 (MQ=255) atAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTcaa > 2:217295/1‑140 (MQ=255) atAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCAGTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAAGCCATGTGCATCTTGTAGGGGTTaaa > 2:275151/1‑140 (MQ=255) gAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGaaa < 1:545921/140‑3 (MQ=255) aaCTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCCTTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACaa < 2:13174/140‑1 (MQ=255) aaCTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACAAACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACaa > 2:381818/1‑140 (MQ=255) tttGTAACGTTGACTTCGGGTGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGATATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGNANGGGTTGAAGATGACAAggg > 1:282124/1‑140 (MQ=255) tttGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAGTgg > 2:296622/1‑140 (MQ=255) tttGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATgg < 2:73409/140‑1 (MQ=255) tGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTTATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTc < 1:240095/140‑1 (MQ=255) gTAATGTTGACTTCGGATGAGTTCAATGCGTTTACTATAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCa < 1:56217/140‑1 (MQ=255) aCTTCGGATGAGTTCAATGCGGTTACTAAAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGc < 2:417169/140‑1 (MQ=255) ggATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTCCAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGAAAATGGTCAAGTGATTGCAGGAt > 2:274906/1‑140 (MQ=255) ggATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTAAACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGAt > 2:275013/1‑140 (MQ=255) aGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGATTa > 2:320399/1‑140 (MQ=255) ttACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAgca < 1:445530/140‑1 (MQ=255) tactacAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAgcac > 2:97141/1‑140 (MQ=255) | GTATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACAACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAGCAC > CP000730/2532164‑2532434 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |