Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730804,6730AT57.4% 18.9 / 67.3 61L135I (TTA→ATA) dtpTPOT family proton (H+)‑dependent oligopeptide transporter
Reads supporting (aligned to +/- strand):  ref base A (20/6);  new base T (6/29);  total (26/35)
Fisher's exact test for biased strand distribution p-value = 4.40e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.85e-01
Rejected as consensus: Frequency below/above cutoff threshold.

CAAAGTGTTGCAAAATAATAGGTGATAATAATGCACCCATATTAACTGACATATAGAAAATAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCACCTAATAAAGTAGCGCCTCTAGTGCCTGTAATTCTGTCAGCAATCCATCC  >  CP000730/804535‑804820
                                                                                                                                          |                                                                                                                                                   
caAAGTGTTGCAAAATAATAGGTGATAATAATGCACCCATATTAACTGACATATAGAAAATAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCa                                                                                                                                           >  2:156593/1‑149 (MQ=255)
           aaaaTAATAGGTGATAATAAAGCACCCATATCAACTGACATAAAGAAAATAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTATACCTGAACCaat                                                                                                                                         <  1:20573/140‑1 (MQ=255)
                  taGGTGATAATAATGCACCCATATTAACTGACATATAGAAAATAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACAATATTTGAAATGTTTGGCTTCATTATTCCTGACCCACTAatgttg                                                                                                                                  >  1:440672/1‑140 (MQ=255)
                       gATAATAATGCACCCATATTAACTGACATATAGAAAATAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGAtgt                                                                                                                    >  2:274011/1‑149 (MQ=255)
                         taataaTGCACCCATATTAACTGACATATAGAAAATAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTATACCTGAACCAATAATGATGAAGAACa                                                                                                                           <  1:693698/140‑1 (MQ=255)
                         taataaTGCACCCAAATTAACTAACAAATAGAAAATAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTATACCTGAACCAATAATGATGAAGAACa                                                                                                                           <  1:572186/140‑1 (MQ=255)
                          aataatGTCCCCATATTAACTGACATATAAAAAATAACAAAACCTGCATCCAGACGGCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTATACCTGAACCAATAATGATGAAGAACAt                                                                                                                          <  1:303606/140‑1 (MQ=255)
                          aataatGCACCCATATTAACGAACATATAGAAAATAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGGTTGGCTTCATTATACCTGAACCAATAATGATGAAGAACAt                                                                                                                          <  1:584754/140‑1 (MQ=255)
                               tGCACCCATATTAACTGACATATAGAAAATAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGc                                                                                                            >  2:51112/1‑149 (MQ=255)
                               tGCACCCATATTAACTGACATATAGAAAATAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGc                                                                                                            >  2:316676/1‑149 (MQ=255)
                                gCACCCAGATTAACTGACGTATAGAAAATAACAAAACCTTCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTATACCTGAACCAATAATGATGAAGAACATTGAtgt                                                                                                                    <  1:715263/140‑1 (MQ=255)
                                     cATATTAACTGACATATAGAAAATAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTATACCTGAACCAATAATGATGAAGAACATTGATGTGAATa                                                                                                               <  1:300786/140‑1 (MQ=255)
                                       tatTAACTGACATATAGAAAATAACAAAACCTGCATCCATACGGCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTAATTATACCTGTACCAATAATGAAGAAGAACATTGATGTGAAtcaa                                                                                                             >  1:695315/1‑137 (MQ=255)
                                          tAACTGACATATAGAAAATAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAGTAAGCCGATTAATGCa                                                                                                 >  2:348206/1‑149 (MQ=255)
                                            aCTGACATATAGAAAATAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCaaa                                                                                               <  2:217295/149‑1 (MQ=255)
                                               gACATATAGAAAATAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACAAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATgg                                                                                            >  2:561682/1‑149 (MQ=255)
                                                aCATATAGAAAATACCAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGt                                                                                           <  2:292328/149‑1 (MQ=255)
                                                     taGAAAATAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCt                                                                                      >  2:79550/1‑149 (MQ=255)
                                                      aGAATATAACATAGCCTGCTACCATACGTCTATCAAGTTCAGGATAGAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTATACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAAt                                                                                              <  1:16832/140‑1 (MQ=255)
                                                      aGAAAATAACAAAGCCTGCATCTATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCtt                                                                                     <  2:258966/149‑1 (MQ=255)
                                                        aaaaTAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTaa                                                                                   >  2:56380/1‑149 (MQ=255)
                                                         aaaTAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTATACCTGAACCAATAAAGAAAAAGAACAACGAAGTGAATAAGCCGATCAATGCCACTgga                                                                                           >  1:990028/1‑139 (MQ=255)
                                                         aaaTAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTATACATGACCCAATAAAAATAAAAAACATTCAAATAAATAAGCCGATTacaccacagtga                                                                                           >  1:127801/1‑129 (MQ=255)
                                                             aaCAAAACCTGCATCCAGACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTATACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGc                                                                                       <  1:50296/140‑1 (MQ=255)
                                                              gcAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATCTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACaaaa                                                                             >  2:304164/2‑148 (MQ=255)
                                                              aCAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAat                                                                             >  2:525710/1‑149 (MQ=255)
                                                               cAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAata                                                                            >  2:403100/1‑149 (MQ=255)
                                                                   aCCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATAGTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCAGATTAATGCAAATGGTAAGCTTACAACAATATgcc                                                                        >  2:445595/1‑147 (MQ=255)
                                                                      cacaacgaTACTTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGa                                                                     <  2:44786/142‑1 (MQ=255)
                                                                       gcaccgagaCGTCCATTATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTATAACTGAACCAAAAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAat                                                                             <  1:794192/132‑1 (MQ=255)
                                                                           ccATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTATACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAACGGTATGCTTAAACAAATATGt                                                                         <  1:517369/140‑1 (MQ=255)
                                                                           ccAAACGTCTATCATTTTCAAGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTATACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAAAGGTAAGCTTAAACAAATATGt                                                                         <  1:520107/140‑1 (MQ=255)
                                                                             aTACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTATACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTcc                                                                       <  1:143894/140‑1 (MQ=255)
                                                                             aTACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGataataat                                                              <  2:87042/149‑1 (MQ=255)
                                                                               aCGTCTATTATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTGTGGCTTCATTATACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGa                                                                     <  1:245627/140‑1 (MQ=255)
                                                                                cGTCTATTATTTTCAGGATATAAACGGCCAACGATATGTGAAATGTTTGGATTCATTAAACATGAACCAATAATGATGAAGAACATTGATGTGAATAAGCAGCTTAATGCAAATGGTAAGCTTAAACAAATATGTCAGCTACAAATaaa                                                           >  2:81117/1‑149 (MQ=255)
                                                                                cGTCGACCATTTTCAGGATAGAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTATACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGTAAACGGTAAGCTTAAACAAATATGTCCGat                                                                    <  1:288846/140‑1 (MQ=255)
                                                                                   cTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTATACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGataat                                                                 <  1:945020/140‑1 (MQ=255)
                                                                                      tCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTACGATAATAAATAAGACTGc                                                     >  2:601783/1‑149 (MQ=255)
                                                                                       cATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGGTAATGATAAAGACCGcc                                                    >  2:260030/1‑148 (MQ=255)
                                                                                          tttCAGGATATAAAGGGCCAAGGATATTTGAAATGTTTGGCATCATTATACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAg                                                          <  1:927748/140‑1 (MQ=255)
                                                                                          tttCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTATACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAg                                                          <  1:918143/140‑1 (MQ=255)
                                                                                            tCAGGATATAAACGGCCAAAGATATTTGAAATGTTTGGCTTCATTATACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGAc                                                        <  1:798583/140‑1 (MQ=255)
                                                                                             cAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTATACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACt                                                       <  1:63357/140‑1 (MQ=255)
                                                                                                     aaaCGGCCAACGATATTTGAAATTTTTGGCTTCATTATACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCACCtaa                                               <  1:804078/140‑1 (MQ=255)
                                                                                                      aaCGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGACCAAACACTGATGAAGACAATTGATGTGAATAAGATCAATCACGAAATTGTTAAAATAAAACCAGCAATGGCGGTAAGAATAAAGACTGCACCTAATAAAGTAgcg                                     >  2:499504/1‑149 (MQ=255)
                                                                                                       aCGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCACCTAATAAAGTAgcgc                                    <  2:271005/149‑1 (MQ=255)
                                                                                                          gCCAACGATATTTGAAATGTTTGGCTTCATTATACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCACCTAATAAAg                                          <  1:532419/140‑1 (MQ=255)
                                                                                                          gCCAACGATATTTGAAATGTTTGGCTTCATTATACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCACCTAATAAAg                                          <  1:631546/140‑1 (MQ=255)
                                                                                                                gATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCACCGAATAAAGTAGCGCCTCTAGTGc                           >  2:44012/1‑149 (MQ=255)
                                                                                                                  tctttGAAGTGTTTGGCTTCATTATACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCACCTAATAAAGTAGCGCct                                  <  1:208842/138‑1 (MQ=255)
                                                                                                                   atTTGAAATGTTTGGCTTCATTATACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCACCTAATAAAGTAGCGCctc                                 <  1:469307/140‑1 (MQ=255)
                                                                                                                     ttGAAATGTTTGGCTTCATTATACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCACCTAATAAAGTAGCGCCTCTa                               <  1:554997/140‑1 (MQ=255)
                                                                                                                      tGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCACCTCATAAAGTAGCGCCCCTAGTGCCTGTaa                     >  2:557144/1‑149 (MQ=255)
                                                                                                                         aaTGTTTGGCTTCGTTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCACCTAATAAAGTAGCGCCTCTAGTGCCTGTAATTc                  >  2:158004/1‑149 (MQ=255)
                                                                                                                             tttGGCTTCATTATACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCACCTAATAAAGTAGCGCCTCTAGTGCCTGt                       <  1:823047/140‑1 (MQ=255)
                                                                                                                              ttGGCTTCATTATACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCACCTAATAAAGTAGCGCCTCTAGTGCCTGTa                      >  1:720372/1‑140 (MQ=255)
                                                                                                                               tgtgttCAGTATACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCACCTAATAAAGTAGCGCCTCTAGTGCCTGTaa                     <  1:467194/136‑1 (MQ=255)
                                                                                                                                ggCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCACCTAATAAAGTAGCGCCTCTAGTGCCTGTAATTCTGTCAGc           >  2:174629/1‑149 (MQ=255)
                                                                                                                                      aTTATACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCACCTAATAAAGTAGCGCCTCTAGTGCCTGTAATTCTGTc              >  1:153763/1‑140 (MQ=255)
                                                                                                                                       ttATACCTGGACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCACCTAATAAAGTAGCGCCTCTAGTGCCTGTAATTCTGTCa             <  1:257122/140‑1 (MQ=255)
                                                                                                                                         aaaCCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGGTAATAATAAAGACTGCACATAATAAAGTAGCGCCTCTAGTGCCTGTAATTCTGTCAGCAatccatcc  >  2:35466/1‑149 (MQ=255)
                                                                                                                                          |                                                                                                                                                   
CAAAGTGTTGCAAAATAATAGGTGATAATAATGCACCCATATTAACTGACATATAGAAAATAACAAAACCTGCATCCATACGTCTATCATTTTCAGGATATAAACGGCCAACGATATTTGAAATGTTTGGCTTCATTAAACCTGAACCAATAATGATGAAGAACATTGATGTGAATAAGCCGATTAATGCAAATGGTAAGCTTAAACAAATATGTCCGATAATAATAAAGACTGCACCTAATAAAGTAGCGCCTCTAGTGCCTGTAATTCTGTCAGCAATCCATCC  >  CP000730/804535‑804820

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑