Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	2,532,298	A→T	T15T (ACA→ACT)	murN →	FemAB family peptidoglycan biosynthesis protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,532,298	0	A	T	100.0%	243.2 / NA	73	T15T (ACA→ACT)	murN	FemAB family peptidoglycan biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (17/56);  total (17/56)

GTGTATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACAACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAGCACGCACACTT  >  CP000730/2532162‑2532442                                                                                                                                         |                                                                                                                                                 gtgtATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCAtttt                                                                                                                                      <  2:2600/149‑1 (MQ=255) gtgtATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCAtttt                                                                                                                                      <  2:232749/149‑1 (MQ=255)   gtATTTGTAATTAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTc                                                                                                                                    <  2:488975/149‑1 (MQ=255)    tATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAg                                                                                                                                            <  1:320714/140‑1 (MQ=255)    tATGTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTTAAAAATTATAGTTCAATTCAGAGAGCGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACCAAg                                                                                                                                            <  1:725563/140‑1 (MQ=255)        tGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCAtt                                                                                                                                        <  1:812032/140‑1 (MQ=255)        tGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCAtt                                                                                                                                        <  1:663409/140‑1 (MQ=255)           aaTCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCAttttt                                                                                                                                     <  1:386687/140‑1 (MQ=255)           aaTCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTaca                                                                                                                            <  2:266130/149‑1 (MQ=255)            aTCAGTTTTTGATAAAATAAAAATATAGCATTATTAATAAATTCAAAGATTATCGTTCCATTCCGAGAGGGATGGGATAAAATGAACTTTGTAACGTTTACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTacac                                                                                                                           <  2:286485/149‑1 (MQ=255)                    ttGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTaca                                                                                                                            <  1:327957/140‑1 (MQ=255)                    ttGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAg                                                                                                                   <  2:133076/149‑1 (MQ=255)                     tGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTacac                                                                                                                           <  1:790238/140‑1 (MQ=255)                     tGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGc                                                                                                                  <  2:195591/149‑1 (MQ=255)                     tGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGc                                                                                                                  <  2:135095/149‑1 (MQ=255)                     tGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGc                                                                                                                  <  2:591451/149‑1 (MQ=255)                     tGACAAAATAAAAAAACAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGCATAAAATGAACTTTGTAACGTTGACTTCGAATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGc                                                                                                                  <  2:150868/149‑1 (MQ=255)                     aaaaaaaataaaaAAATAGCATTATTAATAAATTCAAAAAGTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGc                                                                                                                  <  2:305093/145‑1 (MQ=255)                      gagaaaataaaaAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTacaca                                                                                                                          <  1:517607/137‑1 (MQ=255)                      gATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTGGGATGAGTTCAATTCGTTTTTTACAAAGCATTTTTTTCATTacaca                                                                                                                          >  1:85876/1‑140 (MQ=255)                      gATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTacaca                                                                                                                          >  1:1010822/1‑140 (MQ=255)                         aaaataaaaAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTAtt                                                                                                              <  2:23636/149‑1 (MQ=255)                               aaaaaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCt                                                                                                                 <  1:51994/140‑1 (MQ=255)                                  aaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAAt                                                                                                     <  2:263965/149‑1 (MQ=255)                                  aaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAAt                                                                                                     <  2:530587/149‑1 (MQ=255)                                          ttattaATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCGATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGt                                                                                             >  2:433545/1‑149 (MQ=255)                                                ataaATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGAttt                                                                                       <  2:311736/149‑1 (MQ=255)                                                    aTTCAAAGATTATCGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTGTCACATTACACACAATCAGCTATTCATTACAATCATAGAGtt                                                                                            <  1:629019/140‑1 (MQ=255)                                                      tCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTAGAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGa                                                                                          <  1:671916/140‑1 (MQ=255)                                                      tCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTCCAATCATAGAGTTGa                                                                                          <  1:507276/140‑1 (MQ=255)                                                      tCAAAAAGTATAGTTCAATTCAGAGAGGTATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAgg                                                                                 <  2:225602/149‑1 (MQ=255)                                                          aaaTTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATATCTAACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGGTTTa                                                                                      >  1:435198/1‑140 (MQ=255)                                                              tataGTTCAATTCAGAGAGGGATGGGATAAAGTGAACTTTTGAACGGTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAg                                                                                  <  1:777979/140‑1 (MQ=255)                                                                 aGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCg                                                                               <  1:646254/140‑1 (MQ=255)                                                                   ttCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTATACAAAATAAGCTATTCATTACAATCATAGAGTTAATTTAAACAGCGATGTGCAGCtt                                                                    >  2:61327/1‑149 (MQ=255)                                                                    tCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGAtg                                                                            <  1:471359/140‑1 (MQ=255)                                                                          caaagagGGATGGGATAAAATTGACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGGTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAgggg                                                             <  2:69191/146‑1 (MQ=255)                                                                           agggagGGATGGGATAAAATGATCTTTGTAACTTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCt                                                                     <  1:1002923/137‑1 (MQ=255)                                                                           agagagGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCt                                                                     <  1:373555/140‑1 (MQ=255)                                                                           agagagGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGt                                                            <  2:391549/149‑1 (MQ=255)                                                                            gagagGGATGGGATAAAATGAACTTTGTGACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGtt                                                           <  2:509824/149‑1 (MQ=255)                                                                            gagagGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGAAGAGTTCGATGCTTGTACTATAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCtt                                                                    <  1:190224/140‑1 (MQ=255)                                                                              gagGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTAAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCTTTACACTCATAGAGTTGGTTTAAAAGGCGTTGTGCAtgttgt                                                                  >  1:164499/1‑140 (MQ=255)                                                                                 ggatgggatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAgg                                                               >  1:290934/1‑140 (MQ=255)                                                                                 ggatgggatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAgg                                                               >  1:97925/1‑140 (MQ=255)                                                                                   atgggatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACACTCATAGAGTTGATTTAAGAGGCGAGNGGCATCGTGTAgggg                                                             >  1:1005089/1‑140 (MQ=255)                                                                                    tgggatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGCTGATTTAAAAGGCGATGTCCACTTTGCAGGGGt                                                            >  1:716621/1‑140 (MQ=255)                                                                                      ggatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACa                                                 >  2:466436/1‑149 (MQ=255)                                                                                        aaaaaaGGAACTTTGTAACGTTGACTGCGGATGAGTTCAATGCGGTTACTACAAAGCATTTTTCACATTACACACAAGCAGCTATTCATTACAATCATAGAGGTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAAt                                               <  2:15097/147‑1 (MQ=255)                                                                                          aaaaTGAACTGTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGTCGATGTGCATCTTGTAGGGGTTAAAGATGACAATgg                                             <  2:147257/149‑1 (MQ=255)                                                                                           aaaTGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGt                                            <  2:454006/149‑1 (MQ=255)                                                                                            aaTTAACTTTGGAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATg                                                    <  1:92598/140‑1 (MQ=255)                                                                                            aaTGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATg                                                    <  1:298403/140‑1 (MQ=255)                                                                                             aTGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCa                                          <  2:180712/149‑1 (MQ=255)                                                                                                  cTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATg                                              <  1:89011/140‑1 (MQ=255)                                                                                                    ttGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGCTGACAATGGt                                            >  1:441756/1‑140 (MQ=255)                                                                                                       tAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCa                                >  2:148871/1‑149 (MQ=255)                                                                                                       tAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCa                                >  2:387184/1‑149 (MQ=255)                                                                                                           gtctacctCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTTTAGGGGTGAAAGATGACAATGGTCAAGTGATTGCAgggt                            >  2:221390/8‑147 (MQ=255)                                                                                                           gTTGACTTCGGATGAGTTCAATGCGCTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGAt                            >  2:514195/1‑149 (MQ=255)                                                                                                             tGACTTCGGATGGGTTCAATGCGGTTACTACAAAGCATTTTCCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGc                          <  2:111627/149‑1 (MQ=255)                                                                                                              gACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTg                                  <  1:900923/140‑1 (MQ=255)                                                                                                               acgtCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCtt                        <  2:44305/146‑1 (MQ=255)                                                                                                               aCTTCTGATGAGTTCAATTCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGc                                 <  1:968273/140‑1 (MQ=255)                                                                                                               aCTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGc                                 <  1:455980/140‑1 (MQ=255)                                                                                                                 ttCGGATGAGTGCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTAt                      <  2:144710/149‑1 (MQ=255)                                                                                                                    ggttGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGAt                            <  1:814748/137‑1 (MQ=255)                                                                                                                    ggaggaGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGa                   <  2:316476/149‑1 (MQ=255)                                                                                                                    ggATGAATTCACTGCGTTTACTAAAAAGCATTTTTCACATTACACATAATCAGCTAGTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGAAACTTGTATGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGAt                            <  1:196229/140‑1 (MQ=255)                                                                                                                            tCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTGGATTAAAAAGGCGATGTGCATCCAGTAGGGGTTAAAGATGACAATGGTAAAGGGATTGCAGGATGCTTATTGACAGAAgca           >  2:261503/1‑149 (MQ=255)                                                                                                                                 gCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGGGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAgcacgcac      <  2:484181/149‑1 (MQ=255)                                                                                                                                    tttACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAGCACGCACACt   <  2:476148/149‑1 (MQ=255)                                                                                                                                     ttACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAGCACGCACACtt  <  2:132443/149‑1 (MQ=255)                                                                                                                                         |                                                                                                                                                 GTGTATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACAACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAGCACGCACACTT  >  CP000730/2532162‑2532442

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑