BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,929,862:1	+T	43.2%	intergenic (‑945/‑223)	USA300HOU_1794 ← / → USA300HOU_1796	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,929,862	1	.	T	43.2%	42.7 / inf	183	intergenic (‑945/‑223)	USA300HOU_1794/USA300HOU_1796	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (61/43); new base T (36/42); total (97/86)
Fisher's exact test for biased strand distribution p-value = 1.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.88e-06

Only 100 of 185 total aligned reads displayed.

TAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGC‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCAC  >  CP000730/1929723‑1929997

tAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGta‑t                                                                                                                                             <  1:511575/141‑1 (MQ=255)

          cGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATCTTTGCCGATGATATAATAGGAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGc                                                                                                                                >  2:273750/1‑141 (MQ=255)

                aaTCTATCAGCTAGAATCACATAACTTATCAGAAGCAAGTGACTGTGCTTGTAACTTCGCTCTGCATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGC‑TTAttt                                                                                                                         <  1:365724/141‑1 (MQ=255)

                  tCTATCAGCTAGAATCATATAATTTAGCAGAAGCAAGTGACTGTGCTTTTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGC‑TTATTTTc                                                                                                                       <  2:171522/141‑1 (MQ=255)

                         gCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGC‑TTATTTTC‑GTTcaca                                                                                                               >  2:242692/1‑141 (MQ=255)

                                    ataACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATACTCGCTAAGGCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGC‑TTATTTTC‑GTTCACATCATAGCACCa                                                                                                    <  1:556458/141‑1 (MQ=255)

                                          tATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGC‑TTATTTTC‑GTTCACATCATAGCACCAGtcatc                                                                                              <  1:8121/141‑1 (MQ=255)

                                               atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAA‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGc                                                                                        >  2:463539/15‑141 (MQ=255)

                                               atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAA‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGc                                                                                        >  2:288542/15‑141 (MQ=255)

                                               atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAA‑TCATTTTC‑GTTCACATCATAGCACCAGCCATCAGCGGc                                                                                        >  2:583412/15‑141 (MQ=255)

                                               atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCACCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAA‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGc                                                                                        >  1:508949/15‑141 (MQ=255)

                                                tcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAA‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCt                                                                                       <  1:715230/128‑1 (MQ=255)

                                                tcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑C‑TTTGCTAA‑TCATTTTC‑GTTCCCATCATAGCACCAGTCATCAGTGGcg                                                                                       >  2:328926/14‑140 (MQ=255)

                                                aaGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGC‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGc                                                                                        <  1:652195/141‑1 (MQ=255)

                                                 cggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAA‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCtg                                                                                      >  2:400108/13‑141 (MQ=255)

                                                 aGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGC‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCt                                                                                       >  2:568180/1‑141 (MQ=255)

                                                  gCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGC‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCtg                                                                                      >  2:683064/1‑141 (MQ=255)

                                                   gaagtagatatgCTTGTTTATTCACAGATGATATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGATGAGGTGCATTAC‑T‑T‑TGCTAA‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCtgtg                                                                                    <  2:725921/131‑1 (MQ=255)

                                                    aagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAA‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                                                                                   >  1:434977/10‑141 (MQ=255)

                                                     agtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGGGCA‑TTACT‑T‑TGCTAA‑TCATTTTC‑GTTCACATCATAGCCCCAGTCATCAGTGGCTGTGct                                                                                  >  2:74521/9‑140 (MQ=255)

                                                     aGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGC‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                                                                                   >  2:233191/1‑141 (MQ=255)

                                                     aGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGC‑TTATTTTC‑GTTCACATCATAGCACCAGGCATCAGTGGCtgttc                                                                                   >  2:687506/1‑139 (MQ=255)

                                                       tagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAA‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgt                                                                                <  1:288542/135‑4 (MQ=255)

                                                       tGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGC‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCa                                                                                 >  2:756786/1‑141 (MQ=255)

                                                        gACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGC‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCAt                                                                                <  1:418512/141‑1 (MQ=255)

                                                        gACTGCGTTTGTATACTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTTGTCGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGC‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCAt                                                                                <  1:1077/141‑1 (MQ=255)

                                                        gACCGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATGTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGC‑TTATTTTC‑GTTCACATCATAGCACCAGTCCTCAGTGGCTGTGCCAt                                                                                <  2:702695/141‑1 (MQ=255)

                                                         gatttgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAA‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttg                                                                              <  1:48652/137‑6 (MQ=255)

                                                         gatatgCTTTTAAATTCACAGATGGTATAATAATAGAGCCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAA‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttg                                                                              <  1:272892/137‑6 (MQ=255)

                                                         gatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAA‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttg                                                                              >  1:245916/5‑136 (MQ=255)

                                                         gatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAA‑TCATTTTC‑GTTCACATCATAGCACAAGTCATCAGTGGCTGTGctgttg                                                                              >  1:11691/5‑136 (MQ=255)

                                                           tggccTTGTATATTTGCCCATGATATAATAGTAGTGTCGCCTATCGCTCAGGCGTCAATTTAGACGTAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGC‑TTATTTTC‑GTTCATATCATAGCACCAGTCATCAGTGGCTGTGCCATTGc                                                                             <  2:206841/137‑1 (MQ=255)

                                                            atgCTTGTATATTCACAGATGGTATCATCATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAA‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGt                                                                           >  1:304451/2‑141 (MQ=255)

                                                            atgCTTGTATATTCACAGATGGTATAATACTAGAGGCGCCTATCTCTCAGGCGTCAA‑‑TGGACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAA‑TCATTTTC‑GTTCACATCATAGCCCCAGTCATCAGTGGCTGTGctgttacct                                                                           >  2:658256/2‑133 (MQ=255)

                                                            atgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAA‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGt                                                                           <  2:661469/140‑1 (MQ=255)

                                                             tgCTTGTATATTTGGCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGC‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGt                                                                           <  2:286128/141‑1 (MQ=255)

                                                              gCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAA‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtat                                                                         <  1:704492/141‑3 (MQ=255)

                                                               cTTGTATATTCACAGATGGTATAATAATAGAGGCGCATATCTGTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAA‑TCGTTTTA‑GTTAACATCATAGCACCAGTCATCAGAGACTGTGCTGTTGCGtatt                                                                        >  1:183114/1‑141 (MQ=255)

                                                                 tGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAA‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattat                                                                      >  1:762306/1‑139 (MQ=255)

                                                                  gtatattcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑C‑TTTGCTAA‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattata                                                                     >  2:255734/12‑138 (MQ=255)

                                                                   tataTTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTT‑TGCTAA‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatac                                                                    <  1:384396/141‑8 (MQ=255)

                                                                     tatgcggcGATGATATAATAGTAGAGCCGCCTATCTCTCAGGTGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGC‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCt                                                                   <  1:94473/134‑1 (MQ=255)

                                                                     aattcacagatggtAAAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAA‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACtt                                                                  <  2:357554/133‑1 (MQ=255)

                                                                       ttcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAA‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAt                                                                <  2:641712/135‑1 (MQ=255)

                                                                       ttcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAA‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAt                                                                >  2:482053/7‑141 (MQ=255)

                                                                         tGCCGATGATATAATAGAAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGC‑TTATTTTC‑GTTCACATCATAGCACGAGGCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTAtt                                                               >  1:244006/1‑141 (MQ=255)

                                                                         cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAA‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTg                                                              >  1:744835/5‑141 (MQ=255)

                                                                         cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAA‑TCATTTTC‑GTTCACATCATAGCACTAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTg                                                              >  1:46857/5‑141 (MQ=255)

                                                                         cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAA‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTg                                                              >  2:254977/5‑141 (MQ=255)

                                                                           cagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAA‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGc                                                            >  1:692460/3‑141 (MQ=255)

                                                                           cagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAA‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGc                                                            >  1:493152/3‑141 (MQ=255)

                                                                            agatggtATAATAATCGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGTAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAA‑TCATTTTC‑GTTCACATCATAGCACGAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGACt                                                           >  2:214743/2‑141 (MQ=255)

                                                                              atggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAA‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCtta                                                         <  1:622819/141‑3 (MQ=255)

                                                                               aggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑C‑TTTGCTAA‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttag                                                        <  2:690362/138‑4 (MQ=255)

                                                                                ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAA‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGcatagt                                                       >  1:586731/3‑136 (MQ=255)

                                                                                 gtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGTGAGTTGGGGCA‑TTA‑C‑TTTGCTAA‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttatta                                                      >  1:720412/2‑136 (MQ=255)

                                                                                  tataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGGGGAGGTGCATTCC‑T‑T‑TGCTAA‑TCATTTTCACATCGCCGGA‑AGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaa                                                     >  1:177596/1‑140 (MQ=255)

                                                                                  tataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAA‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaa                                                     <  1:642279/141‑2 (MQ=255)

                                                                                    taataaTAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAA‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAAc                                                   >  2:300211/1‑141 (MQ=255)

                                                                                     aataatAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAA‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACg                                                  >  1:81441/1‑141 (MQ=255)

                                                                                     aataatAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAA‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACg                                                  >  2:173057/1‑141 (MQ=255)

                                                                                      ataatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAA‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc                                                 <  1:608995/137‑1 (MQ=255)

                                                                                      ataCTAGAGTCGCCCACTTCTCACGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTT‑TGCTAA‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc                                                 <  2:597991/141‑1 (MQ=255)

                                                                                       taatagAGTCGCCTATCTCTCGGGCGGCAATT‑‑GACGAAGCGAGAAGGAATAGTTC‑T‑T‑TGCTAA‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa                                                <  1:362513/138‑1 (MQ=255)

                                                                                        aatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAA‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaa                                               <  1:702817/139‑1 (MQ=255)

                                                                                         gtagAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGC‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCaa                                               <  1:103884/141‑1 (MQ=255)

                                                                                         atagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAA‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaac                                              <  1:230485/140‑2 (MQ=255)

                                                                                         atagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAA‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaac                                              <  2:371298/140‑2 (MQ=255)

                                                                                         atagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTT‑TGCTAA‑TAATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaac                                              >  2:195353/2‑140 (MQ=255)

                                                                                           agagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAA‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaa                                            >  1:13729/1‑138 (MQ=255)

                                                                                           agagTCGCCTATCTCTCAGGAGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAA‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaa                                            >  1:466623/1‑138 (MQ=255)

                                                                                             agTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGC‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACa                                           >  1:132538/1‑141 (MQ=255)

                                                                                             agTCGCCTATCTCTCAAGCGTCAATTTAGACGCAGAGAGGAGGTGAA‑TAA‑G‑G‑TGATGC‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCAGTGCGTTTTTTTCTTAGTGGCCAAGTAGACGCAATACa                                           >  1:193572/1‑141 (MQ=255)

                                                                                              gTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAC‑G‑C‑T‑CTGCGTTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAACTAGACGCAATACaa                                          <  1:402258/141‑1 (MQ=255)

                                                                                              gTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAA‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaata                                         >  1:742323/1‑135 (MQ=255)

                                                                                               tCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAA‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataa                                        >  1:653965/1‑134 (MQ=255)

                                                                                                cGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAA‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataat                                       >  2:149529/1‑133 (MQ=255)

                                                                                                 gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAA‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                                      >  2:412835/1‑132 (MQ=255)

                                                                                                 gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAA‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTGTACTTATTGGCTTAGTAAACGCaacaaataatt                                      >  2:499373/1‑132 (MQ=255)

                                                                                                  ccTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGC‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTGTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATa                                      >  1:639477/1‑141 (MQ=255)

                                                                                                  ccTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAA‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatta                                     >  2:266377/1‑131 (MQ=255)

                                                                                                  ccTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAA‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatta                                     >  2:184971/1‑131 (MQ=255)

                                                                                                   cTATCTCTCAGGCGTCAATTTAGACGCCGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGC‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAg                                     >  2:529813/1‑141 (MQ=255)

                                                                                                   cTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGC‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTTAGTAGATGCAATACAAAATAg                                     >  2:712681/1‑141 (MQ=255)

                                                                                                   cTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAA‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaa                                    <  1:627713/141‑12 (MQ=255)

                                                                                                   cTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑G‑T‑TGCTAA‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaa                                    >  1:32603/1‑130 (MQ=255)

                                                                                                     ttctctcAGGCGTTAATTTTGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGC‑TTTTTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTCCTTATTGGCTAAGTAGACGCAATACAAAATAGGt                                   <  2:51561/140‑1 (MQ=255)

                                                                                                     aTCTCCCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAA‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaata                                  <  1:416478/141‑14 (MQ=255)

                                                                                                           cAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGC‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGAccga                             >  1:322778/1‑138 (MQ=255)

                                                                                                           cAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGC‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACata                             >  2:495019/1‑141 (MQ=255)

                                                                                                           cAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGC‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACata                             >  1:259031/1‑141 (MQ=255)

                                                                                                           cAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAC‑TCATTTTA‑GTTCACATCCTAGCACCAGTCATCAGTGGCTGTGCTGTTCCGTATTATACTTATTGGCTTAGTAAACGCAACACATAAttaataggcgac                            >  2:111110/1‑130 (MQ=255)

                                                                                                                  cAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGC‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGc                      <  1:668149/141‑1 (MQ=255)

                                                                                                                  cAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGC‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATACGTGACATATAGCCGc                      >  1:517510/1‑141 (MQ=255)

                                                                                                                      ttAGACGCAGAGGGGAGGTGTA‑TAA‑G‑G‑TGATGC‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCa                  <  1:146770/141‑1 (MQ=255)

                                                                                                                       tAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGC‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGccacaa                 >  1:3115/1‑136 (MQ=255)

                                                                                                                          aCGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGC‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATaa              <  1:27185/141‑1 (MQ=255)

                                                                                                                              agagagGAGGTGTA‑TAA‑G‑G‑TGATGC‑TTATTTTC‑GTTCACAGCATAGCAACAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAAt          >  1:369635/1‑141 (MQ=255)

                                                                                                                               gagagGAGGTGTA‑TAA‑G‑G‑TGATGC‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAACAGGTGACATATAGCCGCACCAATAAAAATc         <  2:582040/141‑1 (MQ=255)

                                                                                                                                      ggTGTA‑TAA‑G‑G‑TGATGC‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCAc  >  2:321042/1‑141 (MQ=255)

TAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGC‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCAC  >  CP000730/1929723‑1929997

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`