BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,015	C→T	28.8%	R179R (AGG→AGA)	tpn ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,015	0	C	T	28.8%	1110.5 / inf	766	R179R (AGG→AGA)	tpn	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base C (264/275); new base T (112/109); total (380/386)
Fisher's exact test for biased strand distribution p-value = 6.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 774 total aligned reads displayed.

GCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATC  >  CP000730/35875‑36155

        tGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctt                                                                                                                                       <  1:433529/141‑1 (MQ=17)

         gCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtcttt                                                                                                                                      <  2:743629/141‑1 (MQ=17)

         gCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACAATCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtcttt                                                                                                                                      >  1:333629/1‑141 (MQ=17)

          cTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtctttc                                                                                                                                     >  1:238871/1‑141 (MQ=11)

          cTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttc                                                                                                                                     <  2:609683/141‑1 (MQ=17)

           tAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttct                                                                                                                                    <  1:167401/141‑1 (MQ=17)

            aGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttctt                                                                                                                                   <  1:199490/141‑1 (MQ=17)

             gCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAACTACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGttttttttt                                                                                                                                  >  2:266376/1‑140 (MQ=11)

                  atgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTT‑ACTTTa                                                                                                                            >  2:257377/1‑141 (MQ=25)

                  atgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTAGTCTTTCTT‑ACTTTa                                                                                                                            >  2:210125/1‑141 (MQ=21)

                   tgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGCTACCTTGTCTTTCTT‑ACTTTaa                                                                                                                           >  2:282789/1‑141 (MQ=21)

                     cTAATTTCGTGGCATGGCGAAAATCCGTAGAGCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTT‑ACTTTAatt                                                                                                                         >  2:481183/1‑140 (MQ=19)

                      tAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGCTATCTTGTCTTTCTT‑ACTTTAatat                                                                                                                        >  2:513995/1‑141 (MQ=11)

                      tAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTT‑ACTTTAatat                                                                                                                        >  1:140191/1‑141 (MQ=21)

                       aaTTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTT‑ACTTTAATATg                                                                                                                       >  2:115156/1‑141 (MQ=25)

                           tCGGGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTT‑ACTTTAATATGACgg                                                                                                                   <  1:282257/141‑1 (MQ=11)

                            cGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTT‑ACTTTAATATGACGGt                                                                                                                  <  1:622410/141‑1 (MQ=38)

                             gTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTT‑ACTTTAATATGACGGTg                                                                                                                 <  1:699087/141‑1 (MQ=38)

                             gTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTGATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTT‑ACTTTAATATGaggttg                                                                                                                 >  2:495778/1‑136 (MQ=255)

                             gTGGCATGGCGAAAATCCGTAGATCCGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTT‑ACTTTAATATGACGGTg                                                                                                                 >  1:100074/1‑141 (MQ=255)

                                  aTGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTT‑ACTTTAATATGACGGTGATCtt                                                                                                            >  1:74125/1‑141 (MQ=255)

                                         aaaTCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAAt                                                                                                     >  2:339669/1‑141 (MQ=38)

                                         aaaTCCGTAGAACTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAAt                                                                                                     >  2:659305/1‑141 (MQ=255)

                                           aTCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAATGa                                                                                                   <  1:84032/141‑1 (MQ=255)

                                           aTCCGTAGATCTGAAGAGACCTGCGGCTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTCTTGATACTTTGATACCTTGTCTTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAATGa                                                                                                   <  1:472069/141‑1 (MQ=255)

                                             ccGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAATGAgg                                                                                                 <  1:45957/141‑1 (MQ=255)

                                               gTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAATGAGGtt                                                                                               >  1:271267/1‑141 (MQ=38)

                                                    tCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCa                                                                                          <  2:222628/141‑1 (MQ=38)

                                                     cTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAg                                                                                         <  1:44323/141‑1 (MQ=255)

                                                        aaGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGata                                                                                      <  1:128739/141‑1 (MQ=38)

                                                        aaGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGata                                                                                      >  1:356354/1‑141 (MQ=38)

                                                        aaGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTAT‑TTTCTTGTCTGGAATATAACGGTGATCTTGCTCAATGAGGTTATTCAGata                                                                                      >  1:553944/1‑141 (MQ=11)

                                                         agagACCTGCTTTTCTTTTTATTTCGTTCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGatat                                                                                     <  2:380416/141‑1 (MQ=11)

                                                            gaCCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTc                                                                                  >  1:91304/1‑141 (MQ=255)

                                                             aCCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCg                                                                                 <  2:756952/141‑1 (MQ=255)

                                                                 acGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGt                                                                             >  2:186332/2‑141 (MQ=255)

                                                                    gTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGCTGTACa                                                                          >  1:252966/1‑141 (MQ=255)

                                                                     ttCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACaa                                                                         <  1:326121/141‑1 (MQ=255)

                                                                      tCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTCAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGGTATTTTGATGTACCAt                                                                        >  1:191572/1‑141 (MQ=11)

                                                                      tCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAAt                                                                        <  2:670484/141‑1 (MQ=255)

                                                                         ttttATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGAc                                                                     <  1:146635/141‑1 (MQ=255)

                                                                           ttATATAGAGCGTAAATAGACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAg                                                                   <  2:297953/141‑1 (MQ=255)

                                                                            tatataGAGCGCAAAGTCTCTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTT‑ACGTTAATATGACGGTGATCGTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGt                                                                  <  2:638338/141‑1 (MQ=14)

                                                                             atataGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTc                                                                 >  1:339936/1‑141 (MQ=255)

                                                                               ttaGAGCGTAAATACACTCAATATCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAg                                                               <  2:648681/140‑1 (MQ=255)

                                                                                taGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAgg                                                              <  1:720003/141‑1 (MQ=255)

                                                                                 agagCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgt                                                             <  1:679566/141‑1 (MQ=255)

                                                                                 agagCGTAAATACACCCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgt                                                             <  1:573889/141‑1 (MQ=255)

                                                                                    gCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgttta                                                          >  1:384516/1‑141 (MQ=255)

                                                                                      gTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGatttaag                                                        >  1:313240/1‑141 (MQ=255)

                                                                                       tAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagt                                                       <  1:16056/141‑1 (MQ=255)

                                                                                         aaTACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagttt                                                     <  1:710814/141‑1 (MQ=255)

                                                                                         aaTACATACAATACCATTTCAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagttt                                                     <  2:36339/141‑1 (MQ=255)

                                                                                         aaTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttcagttt                                                     >  1:211236/1‑141 (MQ=255)

                                                                                          aTACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagttta                                                    >  2:120466/1‑141 (MQ=255)

                                                                                           tACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagtttaa                                                   >  1:304196/1‑141 (MQ=255)

                                                                                           tACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagtttaa                                                   >  2:622464/1‑141 (MQ=255)

                                                                                           tACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTC‑ACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagtttaa                                                   >  1:376419/1‑141 (MQ=255)

                                                                                             cactCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTaaaa                                                 >  2:502878/4‑141 (MQ=255)

                                                                                             cactCAAAACCTTTTAAAGTATTCGTTGCTGTATTGATACTTTGATACCTTGTCTTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCCGTCAGGTTTAAGTTTaaaa                                                 >  1:488436/4‑141 (MQ=14)

                                                                                              actCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAg                                                >  2:169318/3‑141 (MQ=255)

                                                                                              aTTCAATACCTTTTAAAGGATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAg                                                >  1:766766/1‑141 (MQ=255)

                                                                                               ctCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGc                                               <  1:653523/140‑1 (MQ=255)

                                                                                                tCATTACCTGTTAAAGTATTCTTTGGTTTATTGATACTTTGATACCTTGTCTTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCt                                              <  2:148519/141‑1 (MQ=14)

                                                                                                      ccTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAATGAGTTTATTCAGATATTTCGACGTACAATTGCAGCCAGGTTTAAGTTTAAAAGCTTTAAtt                                        >  2:411706/1‑141 (MQ=11)

                                                                                                       cTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTa                                       <  2:317875/141‑1 (MQ=255)

                                                                                                          ttAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTattt                                    >  2:533557/1‑138 (MQ=255)

                                                                                                               gTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGGTTAAGTTTAAAAGCTTTAATTAATTTAAcc                               >  2:543028/1‑141 (MQ=255)

                                                                                                               gTATTCTTTGCCGTATTGATACTTTGATCTCTTGTTTTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAATGGGGTTATTCAGATATTTCGATGTACATGTACACTCAGGTTTAAGTTTGAAGGTTTTAATTATTTTAGcc                               <  2:87454/141‑1 (MQ=11)

                                                                                                                   tttttGCCGTATTGATACTTTGATATCTTGTCTTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTg                           <  2:716214/139‑1 (MQ=255)

                                                                                                                   tCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTg                           <  1:225062/141‑1 (MQ=255)

                                                                                                                    ttttGCCGTATTGATACTTTGATATCTTGTCTTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGc                          <  1:116026/140‑1 (MQ=255)

                                                                                                                    cTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGc                          <  1:24463/141‑1 (MQ=255)

                                                                                                                    cTTTGCTGTATTGATACTTTGATACCTCCTCTTTCTT‑ACTCTAATATGACGGTGATCTTGCTCAATGAGGTTATTCCGATATTTCGATGTACCATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGc                          <  2:730039/141‑1 (MQ=14)

                                                                                                                     tttGCTGTATTGATACTTTGATACCTTGTCTTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCt                         >  2:685884/1‑141 (MQ=255)

                                                                                                                     tttGCTGTATGGATACTTTGATTCCTTGTCTTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACCATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTACCCATTGCt                         <  1:525562/141‑1 (MQ=14)

                                                                                                                      ttGCTGTATTGATACTTTGATACCTTGTCTCTCTT‑ACTTTAATATGACGGTGAACTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTa                        >  2:469043/1‑141 (MQ=255)

                                                                                                                      ttGCCGTATTGATACTTTGATATCTTGTCTTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGAAAGTAAAAGTTAGGTTTAAAAGCTTTAATTATTTTAGCCATTGCtg                        >  1:398715/1‑140 (MQ=14)

                                                                                                                        gCTGTATTGATACTTTGATACCTTGTCTTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTAcc                      <  2:139400/141‑1 (MQ=255)

                                                                                                                         ccttATTGAGACTTTGATACCTTGTCTTTTTT‑ACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCt                     <  2:356354/138‑1 (MQ=14)

                                                                                                                         cTGTATTGATACTTTGATACCTTGTCTTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTTAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCt                     <  2:19359/141‑1 (MQ=255)

                                                                                                                          tGTATTGATACTTTGATACCTTGTCTTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCtt                    <  2:575795/141‑1 (MQ=255)

                                                                                                                          cgTATTGATACTTTGATATCTTGTCTTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAATGATGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTGAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCtt                    >  1:659226/2‑141 (MQ=14)

                                                                                                                           gTATTGATACTTTGATACCTTGTCTTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGCTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGcttccttc                   >  2:450344/1‑141 (MQ=255)

                                                                                                                            tATTTATACTTTGATATCTTTTCTTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCg                  <  2:699844/141‑1 (MQ=14)

                                                                                                                            tATTGATACTTTGATACCTTGTCTTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCg                  >  2:709688/1‑141 (MQ=255)

                                                                                                                              ttGATCCTTTGATACCTTGTCGTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAATGGGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGGTTATGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCggt                >  2:439720/1‑139 (MQ=14)

                                                                                                                               tGATACTTTGATATCTTGTCTTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTg               <  1:646673/141‑1 (MQ=255)

                                                                                                                               tGATACTTTGATACCTTGTCTTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTg               <  2:423570/141‑1 (MQ=255)

                                                                                                                                gATACTTTGATACCTTGTCTTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTCCGTTGa              >  2:395110/1‑141 (MQ=255)

                                                                                                                                  tACTTTGATATCTTGTCTTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAg            >  1:424340/1‑141 (MQ=255)

                                                                                                                                  tACTTTGATACCTTGTCTTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAg            <  1:261178/141‑1 (MQ=255)

                                                                                                                                     tttGATATCTTGTCTTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTg         <  1:543629/141‑1 (MQ=255)

                                                                                                                                      ttGCTACCTTGTCTTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGc        <  1:60086/141‑1 (MQ=255)

                                                                                                                                      ttGATATCTTGTCTTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGc        >  2:242426/1‑141 (MQ=255)

                                                                                                                                         aTACCTTGTCTTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTg     >  1:510645/1‑141 (MQ=255)

                                                                                                                                          tACCTTGTCTTTCTT‑ACTTTAATATGACGGTGGTCTTGCTCAATGAGGTTATTCAGATATTTCGGTGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGa    >  2:562842/1‑141 (MQ=255)

                                                                                                                                          tACCTTGTCTTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGa    <  1:497873/141‑1 (MQ=255)

                                                                                                                                           aCCTTGTCTTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGAt   >  2:587083/1‑141 (MQ=255)

                                                                                                                                            ccTTGTCTTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATc  >  1:581133/1‑141 (MQ=255)

GCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTT‑ACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATC  >  CP000730/35875‑36155

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`