Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,857,839 T→C 6.6% I179V (ATT→GTT)  USA300HOU_1728 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,857,8390TC 6.6% 188.5 / 2.6 80I179V (ATT→GTT) USA300HOU_1728hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (37/37);  new base C (2/4);  total (39/41)
Fisher's exact test for biased strand distribution p-value = 6.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCACTTAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTATTTGATACAGCCACACTTTTATCCGCTTCTGCTTGTGCTT  >  CP000730/1857702‑1857979
                                                                                                                                         |                                                                                                                                             
atCACTTAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATTCCACTGCTTTTGGTTCTTCATTTGTTATAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAAtctc                                                                                                                                            <  2:342540/141‑1 (MQ=255)
atCACTTAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAAtctc                                                                                                                                            <  1:341517/141‑1 (MQ=255)
atCACTTAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAAtctc                                                                                                                                            <  1:341542/141‑1 (MQ=255)
 tCACTTTATTTTTTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGGTTCTTCATTTGTTACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATctct                                                                                                                                           <  2:442418/141‑1 (MQ=255)
   ccTTTAATTTTTTGCACTTGCTTCTTTTTTTATCGGTGCTTGTTGTGCTTTCACTGCCACTGCTTTTGGTTTTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTtg                                                                                                                                         <  2:174994/140‑1 (MQ=17)
   aCTTAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCttt                                                                                                                                         >  1:740103/1‑140 (MQ=255)
    ctttatttgtttGCACTTGCTTCTTTTTTTATTTGTGCTTGTTGTTTTTTCAATGCTACTTCTTTTGGTCTTTCATCTTTTACTGCTGCATTTTTATCTGTCTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTtgt                                                                                                                                        <  2:22107/132‑1 (MQ=12)
     ttAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTtgtg                                                                                                                                       <  1:40002/141‑1 (MQ=255)
       aaaTTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGcc                                                                                                                                     <  1:47849/141‑1 (MQ=255)
         attattTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGccgc                                                                                                                                   >  2:402917/1‑139 (MQ=255)
         attattTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCctt                                                                                                                                   >  2:28670/1‑140 (MQ=255)
          ttttttGCACTTGCTTCTTTTTTCATCTTTGCTTGTGGTGCTGTGAAGTAGTCTGCTTTTGCTTCTTCATTTCTTCCAGCAGCACTTTTACCTGTTTCTGTTTGTGCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCctat                                                                                                                                  <  2:302442/138‑3 (MQ=16)
          ttattTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGGTTCTTCGCGGGTTACAGCCGCAACATGTTGTCTTTCGGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCctct                                                                                                                                  <  1:180909/141‑1 (MQ=14)
          ttattTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGCTTCTGCTTGTTCGTATTTTTTACCTTTTTGAATCTCTtgtgtcggt                                                                                                                                  >  2:74664/1‑136 (MQ=14)
           tattTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGTATCTCTTGTGCCTCtt                                                                                                                                 <  1:457082/141‑1 (MQ=255)
           tattTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCCTTTGGTTCTTCTTTTGATACAGCTGCACTTCTAACTGGTTCTGCTTGTGCTTCTTTTTTATCTTCTTGAGTCTCTTGTGCCTCtt                                                                                                                                 >  2:46940/1‑141 (MQ=14)
             tttGCACTTGCTTCTTCTTTTATCGCTGCTTTTTGTGCTTTTAATGCTACTGCTTTTGGTTCTTCATTTGTTACAGCTGCACTTTTGTCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTtgt                                                                                                                               <  2:32096/141‑1 (MQ=17)
              ttGCACTTGCTTCTTCTTTTGTCGCTGCTTGTTGTGCTTTCAATACCACTGCTTTTGGGTCTTTATTTGATACAGCTGCACTTTTTTCTGTTTCTGCTTGTGCTTCATTTTTAGATTCTTGAATCACTTGTGCCTCTtgtt                                                                                                                              >  1:465825/1‑140 (MQ=14)
                 cccTTTCTTCTTCTTTTCTCTCTGCTTGTTGTGCTTTCACTTCCACTTCTTTTGGTTCTTCATTTTATACAGCTGCACTTTTGTCTTTTTCTGCTTGTTCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATg                                                                                                                           <  2:276994/139‑1 (MQ=17)
                          ttttttttACCGCTGCTTGTTGTGCTTTCAATGCCACTGTTTTTGGTTCTTCATTTTTTATAGCTGCACTTTTATCTTTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCa                                                                                                                  <  2:378800/137‑1 (MQ=17)
                          cttcttTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGGTTTTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCa                                                                                                                  <  2:63017/141‑1 (MQ=255)
                          cttcttTTATCGCTGCTTGTTGTGCTATCAATGCCACTGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCAGCTTGAGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGTCTCTTGTGATGTATCACTCa                                                                                                                  <  1:468061/141‑1 (MQ=255)
                            tcttTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCaaa                                                                                                                <  1:630128/141‑1 (MQ=255)
                                 tATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAattatt                                                                                                           >  1:435498/1‑141 (MQ=255)
                                 tATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCGTATGATGTttcattcaggttagt                                                                                                           >  2:178234/1‑134 (MQ=17)
                                       tgctTGTTGTGCTTTCAATGCCACTGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACt                                                                                                     <  1:146107/141‑1 (MQ=255)
                                         tttgttgTGCCTTCAATGCCCCTGCTTTTGGTTCTTCATTTGATTCTGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTCAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTg                                                                                                   <  2:492310/140‑1 (MQ=25)
                                          ttgttgTGCTTTCACTGCCACTGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGCATCTCTTGTGCCCCTTGTGATGTATCTCTCACattcttccccctcgc                                                                                                  >  2:709246/1‑132 (MQ=37)
                                           tgttgtGCTTTCAATGCCACTGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCTTCTTGTGATGTATCACTCAAATTATTTGCACTTGct                                                                                                 >  2:295273/1‑141 (MQ=255)
                                                tgCTTTCAATGCCACTGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGcttcttc                                                                                            <  1:370140/141‑1 (MQ=255)
                                                  ttttCAGTGCCACTGTTTTTGGTTTTGCATTTGTTTGTGCTGCGCTTTTGTCTTTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGcttcttctt                                                                                         <  2:137864/140‑1 (MQ=17)
                                                     tCAATGCCACTGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTTCTTTTTCTTTTa                                                                                      >  2:523532/1‑141 (MQ=255)
                                                     tCAATGCCACTGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCttttt                                                                                      >  2:313379/1‑140 (MQ=255)
                                                     tCAATGCCACTGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTa                                                                                      >  1:376700/1‑141 (MQ=255)
                                                      cAATGCCGCTGCTTTTGGTTATTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACATGCTTCTTCTTTTAt                                                                                     >  1:480277/1‑141 (MQ=255)
                                                      cAATGCCACTGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTAt                                                                                     >  1:424260/1‑141 (MQ=255)
                                                        aTGCCACTGCTTTTGGTTCTTCATTTGCTACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAACTTATTTGCACTTGCTTCTTCTTTTATCg                                                                                   <  1:301045/141‑1 (MQ=255)
                                                        aTGCCACTGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCg                                                                                   >  1:55661/1‑141 (MQ=255)
                                                            cACTGCTTTTGGTTCTTCATTTGATATGACTTCTCGTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCgctgc                                                                               >  1:372262/1‑141 (MQ=17)
                                                                tcTTTTGCTTCTTCATCTTTTGTTGATGCATTTTTATCTTTCTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCttgt                                                                           <  2:372807/140‑1 (MQ=14)
                                                                    ttGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTtgtg                                                                       >  1:314932/1‑141 (MQ=255)
                                                                        ttcttcATTTGGTACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTTAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCttt                                                                   <  2:625659/141‑1 (MQ=255)
                                                                                  gATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGTTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGATTTTAAttctcct                                                         >  2:108757/1‑135 (MQ=17)
                                                                                  gATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGCATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGcccag                                                         >  2:108771/1‑138 (MQ=255)
                                                                                  gATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACt                                                         >  1:236271/1‑141 (MQ=255)
                                                                                    tgttgatgtgtttttGTCTTTCTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGc                                                       <  2:358586/130‑1 (MQ=11)
                                                                                     aCAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTCATCGCTGCTTGCTGTGCCTTCAATGCCCCtccc                                                      >  2:508689/1‑138 (MQ=17)
                                                                                     aCAGCTGCACTTTTATCTGTCTCTGCTTGTGCTTCTTTTTTAGCATCTTGAATCTCTTGTGCCTCTTGTGATTTATGAATCTACGCACTTGCACTTGCCTCTTCTTTCATCGCTATTTGTTGTGCTTTCGATTCCAGTGCt                                                      >  2:421899/1‑141 (MQ=14)
                                                                                       aGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGAttt                                                    >  1:300029/1‑141 (MQ=255)
                                                                                        gctgtgcTTTTGTCTCTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGCATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCtttt                                                   <  1:348173/135‑1 (MQ=14)
                                                                                        gCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCtttt                                                   >  1:759692/1‑141 (MQ=255)
                                                                                        gCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCtttt                                                   <  1:591257/141‑1 (MQ=255)
                                                                                        gCTGCACTATTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCtttt                                                   <  1:348228/141‑1 (MQ=255)
                                                                                          tgtgcTTTTGTCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTTTTGTATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGa                                                 <  1:204110/137‑1 (MQ=255)
                                                                                           tcatttttGTCTTTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTTAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGAt                                                <  2:158557/137‑1 (MQ=255)
                                                                                                tttATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCTTCTTGTGATGTATCACTTAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCttgt                                                                           <  2:358549/109‑1 (MQ=12)
                                                                                                tttATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCttgt                                                                           >  1:358549/1‑109 (MQ=255)
                                                                                                tttATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTTAATGCCACTTCtttttttttttt                                           >  2:692692/1‑133 (MQ=255)
                                                                                                tttATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCttt                                           <  1:647950/141‑1 (MQ=255)
                                                                                                  tATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCGTCTTGTGATGTATCACTCAAATTCTTTGCACTTGCTTCTTCTTTTGTCGCTGCTTGTTGTGCTTTTAATGCCACTGGTTTTGTttttttct                                         >  1:233385/1‑139 (MQ=14)
                                                                                                   ttCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTAtt                                        <  1:708994/140‑1 (MQ=255)
                                                                                                     ctttttCTGCTTGTGCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTGTGATTCTTTATTTg                                      <  1:411408/138‑1 (MQ=255)
                                                                                                        tttCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCACTGCCACTGCTTTTGATTCTTTATTTGATa                                   >  1:263472/1‑141 (MQ=255)
                                                                                                          tCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTATTTGATACa                                 <  1:450546/141‑1 (MQ=255)
                                                                                                          tCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTCTATTTGATACa                                 <  1:633027/141‑1 (MQ=255)
                                                                                                           ttGCTTGTGCTTCTTTTTTTGCTTCTTGATTCTCTTGTGCCTCTTTTTATGTATCTCTTAAATTATTTGCACTTGCTTCTTCTTTTATCTCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTATTTTATACAg                                <  2:253775/140‑1 (MQ=11)
                                                                                                            tGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTATTTGATACAGc                               >  1:391263/1‑141 (MQ=255)
                                                                                                               ttGTGCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATGTATCACTTAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTATTTGATACAGCcac                            >  1:244983/1‑141 (MQ=21)
                                                                                                                tgtgCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGcttcttc                                                                                            >  1:257006/1‑77 (MQ=255)
                                                                                                                tgtgCTTCTTTTTTAGCATCTTGAATCTCTTGTGCCTCTTGTGATAACTCACTCAAAATATTTGCACTTGCTTCTTCTGTTATCGCTGCTTGTTGTGCCTTCATTGCCTCTGCTTTTGATTATTTATTTGATACAGcaccc                           >  2:129261/1‑137 (MQ=11)
                                                                                                                       tttttttAGCTTCTTGATCCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTATTTGATACAGCCACACTTTTAt                    <  2:244983/140‑1 (MQ=255)
                                                                                                                        tttttgAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTATTTGATACAGCCACACTTTTATc                   <  1:74664/141‑1 (MQ=255)
                                                                                                                           tttAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTGCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTATTTGATACAGCCACACTTTTATCCGc                >  2:614076/1‑141 (MQ=255)
                                                                                                                           tttAGCTTCTTGAACCTCTTGTGCCTCTTGTGATGTATCACTTAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTATTTGAt                                    >  2:254875/1‑121 (MQ=2)
                                                                                                                           tttAGCTTCTTGAACCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTATTTGAt                                    <  1:254875/121‑1 (MQ=255)
                                                                                                                            ttaatttcttGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTATTTGATACAGCCACACTTTTATCCGCt               <  2:305099/136‑1 (MQ=255)
                                                                                                                            ttAGCTTCTTGACTCTCTTGTGCCTTTTGTGATGTATCACTCTAATTATTTGCACTTGCTTTTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTATTTGATACAGCCACACTTTTATCCGCt               <  2:300019/141‑1 (MQ=255)
                                                                                                                            ttAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTATTTGATACAGCCACACTTTTATCCGCt               >  1:291891/1‑141 (MQ=255)
                                                                                                                              aGCTTCTTGAATCTCTTGTGCCGCTTGGTATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTTTTTCTTTTTTTGATGAAGCTCCCCTTTTTTCTGCttt             >  2:426951/1‑140 (MQ=14)
                                                                                                                                    ttGAATCTTTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTTTTCTTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTATTTGATACAGCCACACTTTTATCCGCTTCTGCtt        <  2:397588/141‑1 (MQ=255)
                                                                                                                                       aaTCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCCTTCAATGCCACTGCTTTTGATTCTTTATTTGATACAGCCACACTTTTATCCGCTTCTGCTTGTGc    <  1:105344/141‑1 (MQ=255)
                                                                                                                                        aTCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTTTTTTTTTATTTTATACAGCCACACTTTTACCTGCTTTTGCTTGTGCt   >  2:696072/1‑141 (MQ=14)
                                                                                                                                        aTCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCGTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTATTTGATACAGCCACACTTTTATCCGCTTCTGCTTGTGCt   >  1:304267/1‑141 (MQ=255)
                                                                                                                                         tctcTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTATTTGATACAGCCACACTTTTATCAGCTTCTGCTTGTGCtt  >  1:416772/1‑141 (MQ=255)
                                                                                                                                         tctcTTGTGCCTATTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTATTTGATACAGCCACACTTTTATCCGCTTCTGCTTGTGCtt  >  1:542028/1‑141 (MQ=255)
                                                                                                                                         |                                                                                                                                             
ATCACTTAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTATTTGATACAGCCACACTTTTATCCGCTTCTGCTTGTGCTT  >  CP000730/1857702‑1857979

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: