| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 2,390,270 | T→C | 7.2% | E54G (GAA→GGA) | moaA ← | molybdenum (Mo2+) cofactor biosynthesis protein A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 2,390,270 | 0 | T | C | 7.2% | 181.5 / 3.6 | 66 | E54G (GAA→GGA) | moaA | molybdenum (Mo2+) cofactor biosynthesis protein A |
| Reads supporting (aligned to +/- strand): ref base T (31/29); new base C (2/3); total (33/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.72e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01 | |||||||||||
TATCTTCAATACCATCGATTTGATTTAATTTAGCTATAAGTACATCTAAATCCCGTCGCATCAATGGTTCTCCACCTGTAATGCGTATTTTTTTTACACCTAATTCTGCATATACCTTAGCGATTCTAGCCATTTCATCAAACGTTAAAAGTTCATTTTTAGGTAAAAATACGAAATCATCTCCAAATACCTCTTTAGGCATGCAATAATCACACCTAAAGTTACACCGATCTGTCACAGATAACCGTAAGTCACGGATGGGACGTCCTAGT > CP000730/2390136‑2390407 | tatCTTCAATACCATCGATTTGATTTAATTTAGCTATAAGTACATCTAAATCCCGTCGCATCAATGGTTCTCCACCTGTAATGCGTATTTTTTTTACACCTAATTCTGCATATACCTTAGCGATTCTAGCCATTTCATTaa > 2:140805/1‑141 (MQ=255) atCTTCAATACCATCGATTTGATTTAATTTAGCTATAAGTACATCTAAATCCCGTCGCATCAATGGTTCTCCACCTGTAATGCGTATTTTTTTTACACCTAATTCTGCATATACCTTAGCGATTCTAGCCATTTCATCaaa < 2:441491/141‑1 (MQ=255) atCTTCAATACCATCGATTTGATTTAATTTAGCTATAAGTACATCTAAATCCCGTCGCATCAATGGTTCTCCACCTGTAATGCGTATTTTTTTTACACCTAATTCTGCATATACCTTAGCGATTCTAGCCATTTCATCaaa > 2:491191/1‑141 (MQ=255) tttaaTACCTTCGATTTGATTTAATTTAGCTATAAGTACATCTAAATCCCGTCGCATCAATGGTTCTCCACCTGTAATGCGTATTTTTTTTACGCCTAATTCTGCATATACCTTAGCGATTCTAGCCATTTCATCAAACGt < 2:25200/138‑1 (MQ=255) ttCAATACCTTCGATTTGATTTAATTTAGCTATAAGTACATCTAAATCCCGTCGCATCAATGGTTCTCCACCTGTAATGCGTATTTTTTTTACACCTAATTCTGCATATACCTTAGCGATTCTAGCCATTTCATCAAACGt < 1:277434/141‑1 (MQ=255) ccATCGATTTGATTTAATTTAGCTATAAGTACATCTAAATCCCGTCGCATCAATGGTTCTCCACCTGTAATGCGTATTTTTTTTCCACCTAATTCTGCATATACCTTAGCGATTCTAGCCATTCCATCACACGTTAAAAGt > 2:608207/1‑141 (MQ=255) ccATCGATTTGATTTAATTTAGCTATAAGTACATCTAAATCCCGTCGCATCAATGGTTCTCCACCTGTAATGCGTATTTTTTTTACACCTAATTCTGCATATACCTTAGCGATTCTAGCCATTTCATCAAACGTTAAAAGt > 2:241673/1‑141 (MQ=255) ccATCGATTTGATTTAATTTAGCTATAAGTACATCTAAATCCCGTCGCATCAATGGTTCTCCACCTGTAATGCGTATTTTTTTTACACCTAATTCTGCATATACCTTAGCGATTCTAGCCATTCCATCAAACGTTAAAAGt > 2:515500/1‑141 (MQ=255) cctCGATTTGATTTAATTTAGCTATAAGTACATCTAAATCCCGTCGCATCAATGGTTCTCCACCTGTAATGCGTATTTTTTTTACACCTAATTCTGCATATACCTTAGCGATTCTAGCCATTTCATCAAACGTTAAAAGtt < 1:73012/139‑1 (MQ=255) tttgatttAATTTAGCTATAAGTACATCTAAATCCCGTCGCATCAATGGTTCTCCACCTGTAATGCGTATTTTTTTTACACCTAATTCTGCATATACCTTAGCGATTCTAGCCATTTCATCAAACGTTCAAAGTTCAtttt > 1:375398/1‑141 (MQ=255) tttgatttAATTTAGCTATAAGTACATCTAAATCCCGTCGCATCAATGGTTCTCCACCTGTAATGCGTATTTTTTTTACACCTAATTCTGCATATACCTTAGCGATTCTAGCCATTTCATCAAACGTTAAAAGTTCAtttt > 1:535691/1‑141 (MQ=255) gatttAATTTAGCTATAAGTACATCTACATCCCGTCGCATCAATGGTTCTCCACCTGTAATGCGTATTTTTTTTACACTTAATTCTGCATATACCTTAGCGATTCTAGCCATTTCATCAAACGTTAAAAGTTCAGTTTTAg > 1:756822/1‑141 (MQ=255) gatttAATTTAGCTATAAGTACATCTAAATCCCGTCGCATCAATGGTTCTCCACCTGTAATGCGTATTTTTTTTACACCTAATTCTGCATATACCTTGGCGGTTCTAGCCATTTCATCAACCGTTAAAAGTTCTTTTTTAg > 2:469443/1‑141 (MQ=255) tttaatttaGCTATAAGTACATCTAAATCCCGTCGCATCAATGGTTCTCCACCTGTAATGCGTATTTTTTTTACACCTAATTCTGCATATACCTTAGCGATTCTAGCCATTTCATCAAACGTTAAAAGTTCATTTTTAGGt > 1:581954/1‑141 (MQ=255) aatttaGCTATAAGTACATTTAAATCCCGTCGCATCAATGGTTCTCCACCTGTAATGCGTATTTTTTTTACACCTAATTCTGCATATACCTTAGCGATTCTAGCCATTTCATCAAACGTTAAAAGTTCATTTTTAGGTaaa < 1:477572/141‑1 (MQ=255) aatttaGCTATAAGTACATCTAAATCCCGGCGCATCAATGGTTCTCCCCCTGTAATGCGTATTTTTTTTACACCTAATTCTGCATATACCTTAGCGATTCTAGCCATTTCATCAAACGTTAAAAGTTCATTTTTAGGTaaa < 1:438318/141‑1 (MQ=255) aagttaGCTATCAGTACATCTAAATCCCGTCGCATCAATGGTTCTCCACCTGTAATGCGTATTTTTTTTACACCTAATTCTGCATATACCTTAGCGATTCTAGCCATTTCATCAAACGTTAAAAGTTCATTTTTAGGTaaa < 1:254228/138‑1 (MQ=255) atttaGCTATAAGTACATCTAAATCCCGTCGCATCAATGGTTCTCCACCTGTAATGCGTATTTTTTTTACACCTAATTCTGCATATACCTTAGCGATTCTAGCCATTTCATCAAACGTTAAAAGTTCATTTTTAGGTaaaa > 1:179933/1‑141 (MQ=255) tttaGCTATAAGTACATCTAAATCCCGTCGCATCAATGGTTCTCCACCTGTAATGCGTATTTTTTTTACACCTAATTCTGCATATACCTTAGCTATTCTAGCCATTTCATCAAACGTTAAAAGTTCATTTTTAGGTaaaaa < 2:261878/141‑1 (MQ=255) cTAAATCCCGTCGCATCAATGTTTCTCCACCTGTAATGCGTATTTTTTTTACACCTAATTCTGCATATACCTTAGCGATTCTAGCCATTTCATCAAACGTTAAAAGTTCATTTTTAGGTAAAAATACGAAATCATCTCCaa < 2:437574/141‑1 (MQ=255) cTAAATCCCGTCGCATCAATGGTTCTCCACCTGTAATGCGTATTTTTTTTACACCTAATTCTGCATATACCTTAGCGATTCTAGCCATTTCATCAAACGTTAAAAGTTCATTTTTAGGTAAAAATACGAAATCATCTCCaa < 1:685723/141‑1 (MQ=255) aaTCCCGTCGCATCAATGGTTCTCCACCTGTAATGCGTATTTTTTTTACACCTAATTCTGCATATACCTTAGCGATTCTAGCCATTTCATCAAACGTTAAAAGTTCATTTTTAGGTAAAAATACGAAATCATCTCCAAATa > 1:37071/1‑141 (MQ=255) aaTCCCGTCGCATCAATGGTTCTCCACCTGTAATGCGTA‑TTTTTTTACACCTAATTCTGCATATACCTTAGCGATTCTAGCCATTTCATCAAACGTTACAAGTTCATTTTTAGGTAAAAATACGAAATCATCTCCAAATAc > 2:167750/1‑141 (MQ=255) tCCCGTCGCATGAATGGTTCTCCACCTGTAATGCGTATTTTTTTTACACCTAATTCTGCATATACCTTAGCGATTCTAGCCATTTCATCAGACGTTAAAAGTTCATTTTTAGGTAAAAATACGAAATCATCTCCAAATAcc < 2:367154/141‑1 (MQ=255) tCCCGTCGCATCAACTGTTCTCCACCTGTAATGCGTATTTTTTTTACACCTAATTCTGCATATACCTTAGCGATTCTAGCCATTTCATCAAACCTTAAAAGTTCATTTTTAGGTAAAAATACGAAATCATCTCCAAATAcc < 2:496722/141‑1 (MQ=255) cGTCGCATCAATGGTTCTCCACCTGTAATGCGTATTTTTTTTACACCTAATTCTGCATATACCTTAGCGATTCTAGCCATTTCATCAAACGTTAAAAGTTCATTTTTAGGTAAAAATACGAAATCATCTCCAAATACctct > 1:526864/1‑141 (MQ=255) cGTCGCATCAATGGTTCTCCACCTGTAATGCGTATTTTTTTTACACCTAATTCTGCATATACCTTAGCGATTCTAGCCATTTCATCAAACGTTAAAAGTTCATTTTTAGGTAAAAATACGAAATCATCTCCAAATACctct < 1:166642/141‑1 (MQ=255) gTCGCATCAATGGTTCTCCACCTGTAATGCGTATTTTTTTTACACCTAATTCTGCATATACCTTAGCGATTCTAGCCATTTCATCAAACGTTAAAAGTTCATTTTTAGGTAAAAATACGAAATCATCTCCAAATACCTCtt < 1:449921/141‑1 (MQ=255) gCATCAATGGTTCTCCACCTGTGATGAGTATTTTTTTTACACCTAATTCTGCATATACCTTAGCGATTCTAGCCATGTCATCAGACGTTAAAAGTTCATTTTTAGGTAAAAATACGAAATCATCTCCCAATCCCTCtttgc > 1:742876/1‑139 (MQ=255) acAATGGTTCTCCACCTGTAATGCGTGTTTTTTTTACACCTAATTCTGCATATACCTTAGCGATTCTAGCCATTTCATCAAACGTTAAAAGTTCATTTTTAGGTAAAAATACGAAATCATCTCCAAATACCTCTTTAGGCa < 1:464262/140‑1 (MQ=255) tGGTTCTCCCCCTGTAATGCGTATTTTTTTTACACCTAATTCTGCATATACCTTAGCGATTCTAGCCATTTCATCAAACGTTAAAAGTTCATTTTTAGGTAAAAATACGAAATCATCTCCAAATACCTCTTTAGGCATGCa < 1:660970/141‑1 (MQ=255) ggTTCTCCACCTGTAATGCGTCTTTTTTTTACACCTAATTCTGCATATACCTTAGCGATTCTAGCCATTTCATCAAACGTTAAAAGTTCATTTTTAGGTAAAAATACGAAATCATCTCCAAATACCTCTTTAGGCATGCaa < 2:398929/141‑1 (MQ=255) ggTTCTCCACCTGTAATGCGTATTTTTTTTACACCTAATTCTGCATATACCTTAGCGATTCTAGCCATTTCATCAAACGTTAAAAGTTCATTTTTAGGTAAAAATACGAAATCATCTCCAAATACCTCTTTAGGCATGCaa > 1:660938/1‑141 (MQ=255) ttCTCCACCTGTAATGCGTATTTTTTTTTTTTTTTATTCTGCATATACCTTAACGATTCTAGCCATTTCATCAAACGTTAAAAGTTCATTTTTAGGTAAAAATACGAAACCTACTACAATGACCCATTTCGGCATGCaatt > 2:766516/1‑140 (MQ=255) ttCTCCACCTGTAATGCGTATTTTTTTTACACCTAATTCTGCATATACCTTAGCGATTCTAGCCATTTCATCAAACGTTAAAAGTTCATTTTTAGGTAAAAATACGAAATCATCTCCAAATACCACTTTAGGCATGCaata > 1:29117/1‑141 (MQ=255) ccACCTGTAATGCGTATTTTTTTTACACCTAATTCTGCATATACCTTAGCGATTCTAGCCATTTCATCAAAAGTTAAAAGTTCATTTTTAGGTAAAAATACGAAATCATCTCCAAATACCCCTTTAGGCATGCACTAATcc > 2:273147/1‑140 (MQ=255) cACCTGTAATGCGTATTTTTTTTACACCTAATTCTGCATATACCTTAGCGATTCTAGCCATTTCATCAAACGTTAAAAGTTCATTTTTAGGTAAAAATACGAAATCATCTCCAAATAAACCTTTAGGCATGAAATAATcac > 2:677136/1‑141 (MQ=255) ccTGTAATGCGTATTTTTTTTACACCTAATTCTGCATATACCTTAGCTATTCTAGCCATTTCATCAAACGTTAAAAGTTCATTTTTAAAAAAAAATACGAAATCATCTCCAAATACCTCTTTAGGCATGCAATAATCacac < 2:276452/141‑1 (MQ=255) ccTGTAATGCGTATTTTTTTTACACCTAATTCTGCATATACCTTAGCGATTCTAGCCATTTCATCAAACGTTAAAAGTTCATTTTTAGGTAAAAATACGAAATCATCTCCAAATACCTCTTTAGGCATGCAATAATCacac < 1:459738/141‑1 (MQ=255) gTAATGCGTATTTTTTTTACACCTAATTCTGCATATACCTTAGCGATTCTAGCCATTTCATCAAACGTTAAAAGTTCATTTTTAGGTAAAAATACGAAATCATCTCCAAATACCTCTTTAGGCATGCAATAATCACACCTa < 1:426730/141‑1 (MQ=255) aaTGCGTATTTTTTTTACACCTAATTCTGCATATACCTTAGCGATTCTAGCCATTTCATCAAACGTTAAAAGTTCATTTTTAGGTAAAAATACGAAATCATCTCCAAATACCTCTTTAGGCATGCaaaggtatcacctaaa > 1:4012/1‑127 (MQ=255) tGCGTATTTTTTTTACACCTAATTCTGAATATACCTTCGCGATTCTAGCCATTTCCTCAAACGTTAAAAGTTCATTTTTAGGCAAAAATACGAAATCATATCCACATGCCACTTTAGACATGCACTATACACACCTaaact > 2:750116/1‑139 (MQ=255) gCGTATTTTTTTTACACCTAATTCTGCATATACCTTAGCGATTCTAGCCATTTCATCAAACGTTAAAAGTTCATTTTTAGGTAAAAATACGAAATCATCTCCAAATACCTCTTTAGGCATGCAATAATCACACCTAAAGtt > 1:49285/1‑141 (MQ=255) tACACCTAATTCTGCATATACCTTAGCGATTCTAGCCATTTCATCAAACGTTAAAAGTTCATTTTTAGGTAAAAATACGAAATCATCTCCAAATACCTCTTTAGGCATGCAATAATCACACCTAAAGTTACACCGATCTGt < 2:751806/141‑1 (MQ=255) aaTTCTGCATATACCTTAGCGATTCTAGCCATTTCATCAAACGTTAAAAGTTCATTTTTAGGTAAAAATACGAAATCATCTCCAAATACCTCTTTAGGCATGCAATAATCACACCTACAGTTACACCGATCTGTCACAGAt > 2:607536/1‑141 (MQ=255) agtcggCATATACCTTAGCGATTCTAGCCATTCCATCAAACGTTAAAAGTTCATTTTTAGGTAAAAATACGAAATCATCTCCAAATACCTCTTTAGGCATGCAATAATCACACCTAAAGTTACACCGATCTGTCACAGATa < 1:608207/136‑1 (MQ=255) aTTCTGCATATACCTTAGCGATTCTAGCCATTCCATCAAACGTTAAAAGTTCATTTTTAGGTAAAAATACGAAATCATCTCCAAATACCTCTTTAGGCATGCAATAATCACACCTAAAGTTACACCGATCTGTCACAGATa < 1:515500/141‑1 (MQ=255) tccATATACCTTAGCGATACTAGCCATTGCATCAAACGTTAAAAGTTCATTTTTAGGTAAAAATACGAAATCATCTCCAAATACCTCTTTAGGCATGCAATAATCACACCTAAAGTTACACCGATCTGTCACAGATAACCg < 2:434602/139‑1 (MQ=255) tGCATATACCTTAGCGATTCTAGCCATTTCATCAAACGTTAAAAGTTCATTTTTAGGTAAAAATACGAAATCATCTCCAAATACCTCTTTAGGCATGCAATAATCACACCTAAAGTTCCACCGATCTGTCACAGATAACCg < 2:508474/141‑1 (MQ=255) gCATATACCTTAGCGATTCTAGCCATTTCATCAAACGTTAAAAGTTCATTTTTAGGTAAAAATACGAAATCATCTCCAAATACCTCTTTAGGCATGCAATAATCACACCTAAAGTTACACCGATCTTTCACAAATAACCga > 1:344408/1‑140 (MQ=255) cATATACCTTAGCGATTCTAGCCATTTCATCAAACGTTAAAAGTTCATTTTTAGGTAAAAATACGAAATCATCTCCAAATACCTCTTTAGGCATGCAATAATCACACCTGAAGTTGCACCGACCTGCAACAGATAACTGTa > 1:553040/1‑141 (MQ=255) ataCCTTCGCGATTCTAGCCATTTCATCAAACGTTAAAAGTTCATTTTTAGGTAAAAATACGAAATCATCTCCAAATACCTCTTTAGGCATGCAATAATCACACCTAAAGTTACACCGATCTGTCACAGATAACCGGAAGt < 1:521713/141‑1 (MQ=255) acaCCTTAGCGATTCTAGCCAGTCCATCAAACGTTAAAAGTTCATTTTTAGGTAAAAATACGAAATCATCTCCAAATACCTCTTTAGGCATGCAATAATCACACCTAAAGTTACACCGATCTGTCACAGATAACCGTAAGt < 2:410619/139‑1 (MQ=255) tAGCGATTCTAGCCATTTCATCAAACGTTAAAAGTTCATTTTTAGGTAAAAATACGAAATCATCTCCAAATACCTCTTTAGGCATGCAATAATCACACCTAAAGTTACACCGATCTGTCACAGATAACCGTAAGTCACGGa > 1:134080/1‑141 (MQ=255) tAGCGATTCTAGCCATTTCATCAAACGTTAAAAGTTCATTTTTAGGTAAAAATACGAAATCATCTCCAAATACCTCTTTAGGCATGCAATAATCACACCTAAAGTTACACCGATCTGTCACAGATAACCGTAAGTCACGGa < 1:491191/141‑1 (MQ=255) aGCGATTCTAGCCATTTCAACAAACGTTAAAAGTTCATTTTTAGGTAAAAATACGAAAAAATCTCAAAATACCTCTTTAGACAAGCAATAATCACACCTAAAGTAACACCGATCTGTCACAGATAACCGTAAGTCACGAAt > 2:523145/1‑141 (MQ=255) gATTCTAGCCATTTCATCAAACGTTAAAAGTTCATTTTTAGGTAAAAATACGAAATCATCTCCAAATACCTCTTTAGGCATGCAATAATCACACCTAAAGTTACACCGATCTGTCACAGATAACCGTAAGTCACGGATggg < 2:746334/141‑1 (MQ=255) gATTCTAGCCATTTCATCAAACGTTAAAAGTTCATTTTTAGGTAAAAATACGAAATCATCTCCAAATACCTCTTTAGGCATGCAATAATCACACCTAAAGTTACACCGATCTGTCACAGATAACCGTAAGTCACGGATggg < 1:44023/141‑1 (MQ=255) aTTCTAGCCATTTCATCAAACGTTAAAATTTCATTTCGAGGTAAAAATACGAAATCATCTCCAAATACCTCTTTAGGCATGCAATAATCACACCTAAAGTTACACCGATCTGTCACAGAAAACCGTAAGTCACGGATGGGa < 2:526864/141‑1 (MQ=255) tCTAGCCATTTCATCAAACGTTAAAAGTTCATTTTTAGGTAAAAATACGAAATCATCTCCAAATACCTCTTTAGGCATGCAATAATCACACCTAAAGTTACACCGATCTGTCACAGATAACCGTAAGTCACGGATGGGACg < 2:581954/141‑1 (MQ=255) cTAGCCATTTCATCAAACGTTAAAAGTTCATTTTTAGGTAAAAATACGAAATCATCTCCAAATACCTCTTTAGGCATGCAATAATCACACCTAAAGTTACACCGATCTGTCACAGATAACCGTACGTCACGGATGGGACgc > 2:257989/1‑140 (MQ=255) aGCCATTTCATCAAACGTTAAAAGTTCATTTTTAGGTAAAAATACGAAATCATCTCCAAATACCTCTTTAGGCATGCAATAATCACACCTAAAGTTACACCGATCTGTCACAGATAACCGTAAGTCACGGATGGGACGTcc < 1:241673/141‑1 (MQ=255) gCCATTTCATCAAACGTTAAAAGTTCATTTTTAGGTAAAAATACGAAATCATCTCCAAATACCTCTTTAGGCATGGCAGAAGCACACCTAAAGTTACACCGATCTGTCACAGATAACCGTAAGTCACGGATGGGACGTCCt > 1:751441/1‑141 (MQ=255) ccATTTCATCAAACGTTAAAAGTTCATTTTTAGGTAAAAATACGAAATCATCTCCAAATACCTCTTTAGGCATGCAATAATCACACCTAAAGTTACACCGATCTGTCACAGATAACCGTAAGTCACGGATGGGACGTCCTa > 1:576305/1‑141 (MQ=255) aattCATCAAATGTTAAAAGTTCATTTTTAGGTAAAAATACGAAATCATCCCCAAATACCTCTTTAGGCATGCAATAATCACACCTAAAGTTACACCGATCTGTCACAGATAACCGTAAGTCACGGATGGGACGTCCTAGt < 2:668881/139‑1 (MQ=255) aTTTCATCAAACGTTAAAAGTTCATTTTTAGGTAAAAATACGAAATCATCTCCAAATACCTCTTTAGGCATGCAATAATCACACCTAACGTTACACCGATCTGTCACAGATAACCGTAAGTCACGGATGGGACGTCCTAGt > 1:299090/1‑141 (MQ=255) aTTTCATCAAACGTTAAAAGTTCATTTTTAGGTAAAAATACGAAATCATCTCCAAATACCTCTTTAGGCATGCAATAATCACACCTAAAGTTACACCGATCTGTCACAGATAACCGTAAGTCACGGATGGGACGTCCTAGt > 2:643771/1‑141 (MQ=255) | TATCTTCAATACCATCGATTTGATTTAATTTAGCTATAAGTACATCTAAATCCCGTCGCATCAATGGTTCTCCACCTGTAATGCGTATTTTTTTTACACCTAATTCTGCATATACCTTAGCGATTCTAGCCATTTCATCAAACGTTAAAAGTTCATTTTTAGGTAAAAATACGAAATCATCTCCAAATACCTCTTTAGGCATGCAATAATCACACCTAAAGTTACACCGATCTGTCACAGATAACCGTAAGTCACGGATGGGACGTCCTAGT > CP000730/2390136‑2390407 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |