Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,519,951 T→G 6.5% P227P (CCA→CCC narG ← nitrate reductase alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,519,9510TG 6.5% 181.0 / 5.9 75P227P (CCA→CCCnarGnitrate reductase alpha subunit
Reads supporting (aligned to +/- strand):  ref base T (38/30);  new base G (4/2);  total (43/32)
Fisher's exact test for biased strand distribution p-value = 6.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.38e-01

TACCTTTATAGCGGACTTCAGTCATAAAATGTGCATCCGGAGTACGTGTTAAAGGTACATTAGAGCCCCACATAATAATGTATGATGCGTTATACCAGTCACTTGATTCAGGCACATCTGTTTGCTCTCCCCAAATTTGTGGAGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACTAAGCATTTCACCACCAAGCAAATTGATGAATCGAGCACCTGCTGCATAACTAATCATTGACATCGCTGGAATAGGTGTAAATCCTGCGATTCGATCTGG  >  CP000730/2519812‑2520091
                                                                                                                                           |                                                                                                                                            
tACCTTTATAGCGGACTTCAGTCATAAAATGTGCATCCGGAGTACGTGTTAAAGGTACATTAGAGCCCCACATAATAATGTATGATGCGTTATACCAGTCACTTGATTCAGGCACATCTGTTTGCTCTCCCCAAATTtgtg                                                                                                                                             <  2:495811/141‑1 (MQ=255)
 aCCTTTATAGCGGACTTCAGTCATAAAATGTGCATCCGGAGTACGTGTTAAAGGTACATTAGAGCCCCACATAATAATGTATGATGCGTTATACCAGTCACTTGATTCAGGCACATCTGTTTGCTCTCCCCAAATTTGTgg                                                                                                                                            >  2:574013/1‑141 (MQ=255)
           cGGACTTCAGTCATAAAATGTGCATCCGGAGTACGTGTTAAAGTTACATTAGAGCCCCACATAATAATGTATGATGCGTTATACCAGTCACTTGATTCAGGCACATCTGTTTGCTCTCCCCAAATTTTCGGAGAGGCAcgt                                                                                                                                  >  1:243181/1‑141 (MQ=255)
           cGGACTTCAGTCATAAAATGTGCATCCGGAGTACGTGTTAAAGGTACATTAGAGCCCCACATAATAATGTATGATGCGTTATACCAGTCACTTGATTCAGGCACATCTGTTTGCTCTCCCCAAATTTGTGGAGAGGCAggt                                                                                                                                  <  2:523509/141‑1 (MQ=255)
            ggACTTCAGTCATAAAATGTGCATCCGGAGTACGTGTTAAAGGTACATTAGAGCCCCACATAATAATGTATGATGCGTTATACCAGTCACTTGATTCAGGCACATCTGTTTGCTCTCCCCAAATTTGTGGAGAGGCAggtg                                                                                                                                 <  2:675863/141‑1 (MQ=255)
            ggACTTCAGTCATAAAATGTGCATCCGGAGTACGTGTTAAAGGTACATTAGAGCCCCACATAATAATGTATGATGCGTTATACCAGTAACTTGATTCAGGCACATCTGTTTGCTCTCCCCAAATTTGTGGAGAGGCAggtg                                                                                                                                 >  1:227478/1‑141 (MQ=255)
             gACTTCAGTCATAAAATTTTCATCCTGAGTACGTGTTAAAGGTAAATTATAGACGCACATAATAATGTATGATCCGTTATACCAGTCACTTGATTCAGGCACATCTGTTTGCTCTCCCCAAATTTGTGGAGAGGCAggtgg                                                                                                                                <  1:716705/141‑1 (MQ=255)
             gACTTCAGTCATAAAATGTGCATCCGGAGTACGTGTTAAAGGTACATTAGAGCCCCACATAATAATGTATGATGCGTTATACCAGTCACTTGATTCAGGCACATCTGTTTGCTCTCCCCAAATTTGTGGAGAGGCAggtgg                                                                                                                                <  2:188452/141‑1 (MQ=255)
              acatgaGTCATAAACTGTGCTTCCGGAGTAAGTGTTAAAGGTATTGTAGAGGCCCCAATAACAACGTATGATGGGTTATACCTTTTACTTGATTCCGGTTTATATGTTTGCTCTCCCCATATTTGTGGAGAGGCAggtggt                                                                                                                               >  2:217451/6‑141 (MQ=255)
                ttCAGTCATAAAATGTGCATCCGGAGTACGTGTTAAAGGTACATTAGAGCCCCACATAATAATGTATGATGCGTTATACCAGTCACTTGATTCAGGCACATCTGTTTGCTCTCCCCAAATTTGTGGAGAGGCAGGTGGTaa                                                                                                                             >  2:610366/1‑141 (MQ=255)
                 tCAGTCATAAAATGTGCATCCGGAGTACGTGTTAAAGGTACATTAGAGCCCCACATAATAATGTATGATGCGTTATACCAGTCACTTGATTCAGGCACATCTGTTTGCTCTCCCCAACTTTGTGGAGAGGCAGGTGGTaaa                                                                                                                            >  2:746080/1‑141 (MQ=255)
                   aGTCATAAAATGTGCATCCGGAGTACGTGTTAAAGGTACATTAGAGCCCCACATAATAATGTATGATGCGTTATACCAGTCACTTGATTCAGGCACATCTGTTTGCTCTCCCCAAATTTGTGGAGAGGCAGGTGGTAAATc                                                                                                                          <  1:583553/141‑1 (MQ=255)
                   aGTCATAAAATGTGCATCCGGAGTACGTGTTAAAGGTACATTAGAGCCCCACATAATAATGTATGATGCGTTATACCAGTCACTTGATTCAGGCACATCTGTTTGCTCTCCCCAAATTTGTGGAGAGGCAGGTGGTAAATc                                                                                                                          >  2:106168/1‑141 (MQ=255)
                     tCATAAAATGTGCATCCGGAGTACGTGTTAAAGGTACATTAGAGCCCCACATAAGAATGTATGATGCGTTATACCAGTCACTTGATTCAGGCACATCTGTTTGCTCTCCCCAAATTTGTGGAGAGGCAGGTGGTAAATCTg                                                                                                                        <  2:341712/141‑1 (MQ=255)
                         aaaaTGTGCATCCGGAGTACGTGTTAAAGGTACATTAGAGCCCCACATAATAATGTATGATGCGTTATACCAGTCACTTGATTCAGGCACATCTGTTTGCTCTCCCCAAATTTGTGGAGAGGCAGGTGGTAAATCTGCATa                                                                                                                    >  1:650998/1‑141 (MQ=255)
                             tgtgCATCCGGAGTACGTGTTAAAGGTACATTAGAGTCCCACATAATAATGTATGATGCGTTATACCAGTCACTTGATTCAGGCACATCTGTTTGCTCTCCCCAAATTTGTGGAGAGGCAGGTGGTCAATCTGCATACCAg                                                                                                                <  1:425495/141‑1 (MQ=255)
                             tgtgCATCCGGAGTACGTGTTAAAGGTACATTAGAGCCCCACATAATAATGTATGATGCGTTATACCAGTCACTTGATTCAGGCACATCTGTTTGCTCTCCCCAAATTTGTGGAGAGGCAGGTGGTAAATCTGCATACCAg                                                                                                                >  2:481457/1‑141 (MQ=255)
                              gtgCATCCGGAGTACGTGTTAAAGGTACATTAGAGCCCCACATAATAATGTATGATGCGTTATACCAGTCACTTGATTCAGGCACATCTGTTTGCTCTCCCCAAATTTGTGGAGAGGCAGGTGGTAAATCTGCATACCAGt                                                                                                               >  2:670094/1‑141 (MQ=255)
                                 cATCCGGAGTACGTGTTAAAGGTACATTAGAGCCCCACATAATAATGTATGATGCGTTATACCAGTCACTTGATTCAGGCACATCTGTTTGCTCTCCCCAAATTTGTGGAGAGGCAGGTGGTAAATCTGCATACCAGTCAt                                                                                                            <  2:489268/141‑1 (MQ=255)
                                  aTCCGGAGTACGTGTTAAAGGTACATTAGAGCCCCACATAATAATGTATGATGCGTTATACCAGTCACTTGATTCAGGCACATCTGTTTGCTCTCCCCAAATTTGTGGAGAGGCAGGTGGTAAATCTGCATACCAGTCATa                                                                                                           >  2:98708/1‑141 (MQ=255)
                                  aTCCGGAGTACGTGTTAAAGGTACATTAGAGCCCCACATAATAATGTATGATGCGTTATACCAGTCACTTGATTCAGGCACATCTGTTTGCTCTCCCCAAATTTGTGGAGAGGCAGGTGGTAAATCTGCATACCAGTCATa                                                                                                           >  1:230777/1‑141 (MQ=255)
                                             gtgtTAAAGGTACATTAGAGCCCCACATAATAATGTATGATGCGTTATACCAGTCACTTGATTCAGGCACATCTGTTTGCTCTCCCCAAATTTGTGGAGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACTAAGCa                                                                                                >  2:584068/1‑141 (MQ=255)
                                                ttAAAGGTACATTAGAGCCCCACATAATAATGTATGATGCGTTATACCAGTCACTTGATTCAGGCACATCTGTTTGCTCTCCCCAAATTTGTGGAGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACTAAGCAttt                                                                                             >  1:352543/1‑141 (MQ=255)
                                                       tACATTAGGGCCGCCCATAATCATGTATGATGCCTTATACCAGTCACTTGATTCAGGCACACCTGTTTGCTCTCCCCAAATTTGTGGAGAGGCAGGTGGTAAATCTGCATACCAGTCATAACAACTAAGCATTTCaccacc                                                                                      <  2:430964/141‑1 (MQ=255)
                                                       tACATTAGAGCCCCACATAATAATGTATGATGCGTTATACCAGTCACTTGATTCAGGCACATCTGTTTGCTCTCCCCAAATTTGTGGAGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACTAAGCATTTCaccacc                                                                                      <  1:627575/141‑1 (MQ=255)
                                                           ttAGAGCCCCACATAATAATGTATGATGCGTTATACCAGTCACTTGATTCAGGCACATCTGTTTGCTCTCCCCAAATTTGTGGAGGGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACTAAGCATTTCACCACCAAGc                                                                                  >  1:732486/1‑141 (MQ=255)
                                                            tAGAGCCCCACATAATAATGTATGATGCGTTATACCAGTCACTTGATTCAGGCACATCTGTTTGCTCTCCCCAAATTTGTGGAGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACTAAGCATTTCACCACCAAGCa                                                                                 <  1:42210/141‑1 (MQ=255)
                                                               ggCCCTACATAATAATCTATGATGCGTTATACCAGTCACTTGATTCAGGCACATCTGTTTGCTCTCCCCAAATTTGTGGAGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACTAAGCATTTCACCACCAAGCAAAt                                                                              <  2:248407/140‑1 (MQ=255)
                                                                 ccccACATAATAATGTATGATGCGTTATACCAGTCACTTGATTCAGGCACATCTGTTTGCTCTCCCCAAATTTGTGGAGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACTAAGCATTTCACCCCCAAGCAAATtg                                                                            >  1:429850/1‑141 (MQ=255)
                                                                 ccccACATAATAATGTATGATGCGTTATACCAGTCACTTGATTCAGGCACATCTGTTTGCTCTCCCCAAATTTGTGGAGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACTAAGCATTTCACCACCAAGCAAATtg                                                                            >  2:574881/1‑141 (MQ=255)
                                                                      caTAATAATGTATGATGCGTTATACCAGTCACTTGATTCAGGCACATCTGTTTGCTCTCCCCAAATTTGTGGAGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACTAAGCATTTCACCACCAAGCAAATTGATGaa                                                                       >  1:535575/1‑141 (MQ=255)
                                                                      caTAATAATGTATGATGCGTTATACCAGTCACTTGATTCAGGCACATCTGTTTGCTCTCCCCAAATTTGTGGAGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACTAAGCATTTCACCACCAAGCAAATTGATGaa                                                                       >  1:19916/1‑141 (MQ=255)
                                                                        taataaTGTATGATGCGTTATACCAGTCACTTGATTCAGGCACATCTGTTTGCTCTCCCCAAATTTGTGGAGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACTAAGCATTTCACCACCAAGCAAATTGATGAATc                                                                     >  2:12609/1‑141 (MQ=255)
                                                                          ataatGTATGATGCGTTATACCAGTCACTTGATTCAGGCACATCTGTTTGCTCTCCCCAAATTTGTGGAGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACTAAGCATTTCACCACCAAGCAAATTGATGAATCGa                                                                   >  1:471695/1‑141 (MQ=255)
                                                                             aggtgtgatgCGTTATACCAGTCACTTGATCCAGGCACATCTGTTTGCTCTCCCCAAATTTGTGGAGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACTAAGCATTTCACCACCAAGCAAATTGATGAATCGAGCa                                                                <  1:94441/136‑1 (MQ=255)
                                                                              tttATGATGCGTTATACCAGTCACTTGATTCAGGCACATCTGTTTGCTCTCCCCAAATTTGTGGAGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACTAAGCATTTCACCACCAAGCAAATTGATGAATCGAGCAc                                                               <  1:13309/139‑1 (MQ=255)
                                                                                tATGATGCGTTATACCAGTCACTTGATTCAGGCACATCTGTTTGCTCTCCCCAAATTTGTGGAGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACTAAGCATTTCACCACCAAGCAAATTGATGAATCGAGCACct                                                             <  2:230777/141‑1 (MQ=255)
                                                                                   gatgCGTTATACCAGTCACTTGATTCAGGCCCCTCTGTTTCCTCTCCCCAAATTTGTGGAGAGGCCGGTGGTAAATCTGCATACCAGTCATAAAAACTAAGCATTCCACCCCCACGCAGATCGAGGCATCCAGCAcaccct                                                          >  2:363368/1‑136 (MQ=255)
                                                                                     tgCGTTATACCAGTCACTTGATTCAGGCACATCTGTTTGCTCTCCCCAAATTTGTGGAGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACTAAGCATTTCACCACCAAGCAAATTGATGAATCGAGCACCtgctgc                                                        >  2:552870/1‑141 (MQ=255)
                                                                                      gCGTTATACCAGTCACTTGATTCAGGCACATCTGTTTGCTCTCCCCAAATTTGTGGAGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACTAAGCATTTCACCACCAAGCAAATTGATGAATCGAGCACCTGCTGCa                                                       >  2:682866/1‑141 (MQ=255)
                                                                                      gCGTTATACCAGTCACTTGATTCAGGCACATCTGTTTGCTCTCCCCAAATTGGTGGAGAGGCAGGTGGTAAATCAGAAAATATTTCATAAAAACTAAGCATTTCACCACCAAGCAAATTGATGAATCGAGCACCTGCTGCa                                                       >  1:750889/1‑141 (MQ=255)
                                                                                         ttACACCAGTCGCTTGATTCAGGCACATCGGTTTGTTCTCCCCAAATTTGTGGAGAGGCAGGTGGTAAATCTGAATACCAGTCATAAAAACTATGCATTTCACCACCAAGCAAATTGATGAATCGAGCACCTGCTGCATaa                                                    <  2:493550/141‑1 (MQ=255)
                                                                                          tataCCAGTCACTTGATTCAGGCACATCTGTTTGCTCTCCCCAAATTTGTGGAGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACTAAGCATTTCAACGCCAAGCAAATTGATGAATCGAGCACCTGCTGCATAAc                                                   <  1:486526/141‑1 (MQ=255)
                                                                                            taCCAGTCACTTGATTCAGGCACATCTGTTTGCTCTCCCCAAATTTGTGGAGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACTAAGCATTTCACCACCAAGCAAATTGATGAATCGAGCACCTGCTGCATAACTa                                                 >  1:243850/1‑141 (MQ=255)
                                                                                             aCCAGTCACTTGATTCAGGCACATCTGTTTGCTCTCCCCAGATTTGTGGAGAGGCAGGTGTTAAATCTGCATACCAGTCATAAAAACTAAGCATTTCACCACCAAGCAAATTGATGAATCTAGCACCTACTGCATAACTaa                                                >  1:425059/1‑141 (MQ=255)
                                                                                             aCCAGTCACTTGATTCAGGCACATCTGTTTGCTCTCCCCAAATTTGTGGAGCGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACCAAGCATTTCACCACCAAGCTAATTGAGGAATCGAGCACCTGCTGCATAACTaa                                                >  2:422859/1‑141 (MQ=255)
                                                                                              ccAGTCGCTTGATTCAGGCACATCTGTTTGCTCTCCCCAAATTTGTGGAGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAACCTAAGCATTTCACCACCAAGCAAATTGATGAATCGAGCACCTGCTGCATAACTAAt                                               <  2:615274/141‑1 (MQ=255)
                                                                                               cAGTCGCTTGATTCAGGCACATCTGTTTGCTCTCCCCAAATTTGTGGAGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACTAAGCATTTCACCACCAAGCAAATTGATGAATCGAGCACCTGCTGCATAACTAATc                                              >  1:502280/1‑141 (MQ=255)
                                                                                                   cACTTGATTCAGGCACATCTGTTTGCTCTCCCCAAATTTGTGGAGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACTAAGCATTTCACCACCAAGCAAATTGATGAATCGAGCACCTGCTGCATAACTAATCATTg                                          <  1:500971/141‑1 (MQ=255)
                                                                                                        gATTCAGGCACATCTGTTTGCTCTCCCCAAATTTGTGGAGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACTAAGCATTTCACCACCAAGCAAATTGATGAATCGAGCACCTGCTGCGTAACTAATCATTGACATc                                     >  1:226806/1‑141 (MQ=255)
                                                                                                        gATTCAGGCACATCTGTTTGCTCTCCCCAAATTTGTGGAGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACTAAGCATTTCACCACCAAGCAAATTGATGAATCGAGCACCTGCTGCATAACTAATCATTGACATc                                     >  1:286030/1‑141 (MQ=255)
                                                                                                         tttCAGGCACATCTGTTTGCTCTCCCCAAATTTGTGGAGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACTAAGCATTTCACCACCAAGCAAATTGATGAATCGAGCACCTGCTGCATAACTAATCATTGACATCg                                    <  1:376616/140‑1 (MQ=255)
                                                                                                         tttCAGGCACATCTGTCTGCTCTCCCCAAATTTGTGGAGAGGCAGGTGGTAAATCCGCATACCAGTCATAAAAACTAAGCATTTCACCACCAAGCAAATTGATGAATCGAGCACCTGCTGCATAACTAATCATTGACATCg                                    <  1:217451/140‑1 (MQ=255)
                                                                                                         tgtCAGGCACATTTGTTTGCTCTCCCCAAATTTGTGGAGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACTAAGCATTTCACCACCAAGCAAATTGATGAATCGAGCACCTGCTGCATAACTAATCATTGACATCg                                    <  2:255354/139‑1 (MQ=255)
                                                                                                          ttCAGGCACATCTGTTTGCTCTCCCCAAATTTGTGGAGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACTAAGCATTTCACCACCAAGCAAATTGATGAATCGAGCACCTGCTGCATAACTAATCATTGACATCGc                                   >  2:742602/1‑141 (MQ=255)
                                                                                                             aGGCACATCTGTTTGCTCTCCCCAAATTTGTGGAGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACTAAGCATTTCACCACCAAGCAAATTGATGAATCGAGCACCTGCTGCATAACTAATCATTGAACTCCCTgg                                >  2:581344/1‑141 (MQ=255)
                                                                                                                  caTCTGTTTGCTCTCCCCAAATTTGTGGAGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACTAAGCATTTCACCACCAAGTACATTAATGTATCGGGCACCTGCTGCATAACTAATCATTGACATCGCTGGaaggg                           >  1:249843/1‑138 (MQ=255)
                                                                                                                  caTCTGTTTGCTCTCCCCAAATTTGTGGAGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACTAAGCATTTCACCACCAAGCAAATTGATGAATCGAGCACCTGCTGCATAACTAATCATTGACATCGCTGGAATAg                           >  2:752527/1‑141 (MQ=255)
                                                                                                                  caTCTGTTTGCTCTCCCCAAATTTGTGGAGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACTAAGCATTTCACCACCAAGCAAATTGATGAATCGAGCACCTGCTGCATAACTAATCATTGACATCGCTGGAATAg                           <  2:761703/141‑1 (MQ=255)
                                                                                                                    tCTGTTTGCTCTCCCCAAATTTGTGGAGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACTAAGCATTTCACCACCAAGCAAATTGATGAATCGAGCACCTGCTGCATAACTAATCATTGACATCGCTGGAATAGgt                         >  2:751607/1‑141 (MQ=255)
                                                                                                                     cTGTTTGCCCTCCCCAAATTTGTGGAGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACTAAGCATTTCACCACCAAGCAAATTGATGAATCGAGCACCTGCTGCATAACTAATCATTGACATCGCTGGAATAGgtg                        <  1:247537/141‑1 (MQ=255)
                                                                                                                      tGTTTGCTCTCCCCAGATTTGTGGAGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACTAAGCATTTCACCACCAAGCAAATCGATGAATCGAGCACCTGCTGCATAACTAATCATTGACATCGCTGGAATAGgtgt                       <  1:512347/141‑1 (MQ=255)
                                                                                                                      tGTTTGCTCTCCCCAAATTTGTGGAGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACTAAGCATTTCACCACCAAGCAAATTGATGAATCGAGCACCTGCTGCATAACTAATCATTGACATCGCTGGAATAGgtgt                       >  2:746815/1‑141 (MQ=255)
                                                                                                                          tGCTCTCCCCAAATTTGTGGAGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACTAAGCATTTCACCACCAAGCAAATTGATGAATCGGGCACCGGCTGCATAACTAATCATTGACATCGCTGGAATAGGTGTAAAt                   >  1:363148/1‑141 (MQ=255)
                                                                                                                          gcctctCCACAAATTTGGGGAGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACTAAGCATTTCACCACCAAGCAAATTGATGAATCGAGCACCTGCTGCATAACTAATAATTGACATCGCTGGAATAGGTGTaaaa                   >  1:36494/3‑140 (MQ=255)
                                                                                                                            ctctCCCCAAATTTGTGGAGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACTACGCATTTCACCACCACGCAAATTGATGAATCGCGAACCTCCTGCAACACTCATCGTGAACATCGCTGGATTGGGTGTTAATcc                 >  2:527124/1‑141 (MQ=255)
                                                                                                                              ctcCCCAAATTTGTGGAGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACTAAGCATTTCACCACCAAGCAAATTGATGAATAGAGAACCGGCTTCATAACTAATCATTGACAGCGCTGGAATAGGTGTAAATCCTg               <  2:277082/141‑1 (MQ=255)
                                                                                                                                   cAAATTTGTGGAGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACTAAGCATTTCACCACCAAGCAAATTGATGAATCGAGCACCTGCTGCATAACTAATCATTGACATCGCTGGAATAGGTGTAAATCCTGCGAtt          <  1:577911/141‑1 (MQ=255)
                                                                                                                                    aaaTTTGTGGAGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACTAAGCATTTCACCACCAAGCAAATTGATGAATCGAGCACCTGCTGCATAACTAATCATTGACATCGCTGGAATAGGTGTAAATCCTGCGATTc         <  2:370601/141‑1 (MQ=255)
                                                                                                                                    aaaTTTGGGGAGAGGGAGGTGGTAAATCTGCATACCAGTCATAAAAACTAAGCATTTCACCACCAAGCAAATTGATGAATCGAGCACCTGCTGCATAACTAATCATTGACATCGCTGGAATAGGTGTAAATCCTGCGATTc         >  1:385272/1‑141 (MQ=255)
                                                                                                                                     aaTTTGTGGAGAGGCAGGTGGTAAATCTGCATACCAGGCATAAAAACTAAGCATTTCACCACCAAGCAAATTGATGAATCGAGCACCTGCTGCATAACTAATCATTGACATCGCTGGAATAGGTGTAAATCCTGCGATTCg        <  2:58260/141‑1 (MQ=255)
                                                                                                                                     aaTTTGGGGGGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACGAAGCATTGCACCACCAAGCAAATTGATGAATCGAGCACCTGCTGCATAACTAATCATTGACATCGCTGGAATAGGTGTAAATCCTGCGATTCg        <  2:80426/141‑1 (MQ=255)
                                                                                                                                     aaTTTGGGGAGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACTAAGCATTTCACCACCAAGCAAATTGATGAATCGAGCACCTGCTGCATAACTAATCATTGACATCGCTGGAATAGGTGTAAATCCTGCGATTCg        >  1:762620/1‑141 (MQ=255)
                                                                                                                                     aaTTTGGCGAGAGGCAGGTGGGAAAACTGCATACCAGTCATAAAAACTAAGCATTTCACCACCAAGCAAATTGATGAATCGAGCACCTGCTGCATAACTAATCATTGACATCGCTGGAATAGGTGTAAATCCTGCGATTCg        <  2:237330/141‑1 (MQ=255)
                                                                                                                                      aTTTGTGGAGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACTAAGCATTTCACCACCAAGCAAATTGATGAATCGAGCACCTGCTGCATAACTAATCATTGACATCGCTGGAATAGGTGTAAATCCTGCGATTCGa       <  1:98708/141‑1 (MQ=255)
                                                                                                                                      aTTTGGGGAGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACTAAGCATTTCACCACCAAGCAAATTGATGAATCGAGCACCTGCTGCATAACTAATCATTGACATCGCTGGAATAGGTGTAAATCCTGCGATTCGa       >  2:38364/1‑141 (MQ=255)
                                                                                                                                         tgtgGAGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACTAAGCATTTCACCACCAAGCAAATTGATGAATCGAGCACCTGCTGCATAACTAATCATTGACATCGCTGGAATAGGTGTAAATCCTGCGATTCGATCt    <  1:633406/141‑1 (MQ=255)
                                                                                                                                          gtgGAGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACTAAGCATTTCACCACCAAGCAAATTGATGAATCGAGCACCTGCTGCATAACTAATCATTGACATCGCTGGAATAGGTGTAAATCCTGCGATTCGATCTg   >  2:262269/1‑141 (MQ=255)
                                                                                                                                           tgGAGAGGCAGGTGGTAGATCTGCATACCAGTCGAAAGAACGAACTATTTGACAAGCATGCAAATTGATGAATCGAGCACCTGCTGCATAACTAATCATTGACATCGCTGGGATAGGTGTAAATCCTGCGATTCGATCTgg  >  1:369403/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                            
TACCTTTATAGCGGACTTCAGTCATAAAATGTGCATCCGGAGTACGTGTTAAAGGTACATTAGAGCCCCACATAATAATGTATGATGCGTTATACCAGTCACTTGATTCAGGCACATCTGTTTGCTCTCCCCAAATTTGTGGAGAGGCAGGTGGTAAATCTGCATACCAGTCATAAAAACTAAGCATTTCACCACCAAGCAAATTGATGAATCGAGCACCTGCTGCATAACTAATCATTGACATCGCTGGAATAGGTGTAAATCCTGCGATTCGATCTGG  >  CP000730/2519812‑2520091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: