Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 35,997 T→C 29.1% T185T (ACA→ACG)  tpn ← IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073035,9970TC29.1% 955.1 / inf 732T185T (ACA→ACG) tpnIS431mec transposase
Reads supporting (aligned to +/- strand):  ref base T (243/274);  new base C (113/99);  total (358/374)
Fisher's exact test for biased strand distribution p-value = 1.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 735 total aligned reads displayed.

TGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTAT‑TCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTT  >  CP000730/35864‑36137
                                                                                                                                      |                                                                                                                                            
   cAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTATAGTAT‑TCTTTGCTGTATTGATAc                                                                                                                                    >  1:743823/1‑141 (MQ=2)
   cAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTAT‑TCTTTGCTGTATTGATAc                                                                                                                                    <  2:698898/141‑1 (MQ=11)
   cAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTAT‑TCTTTGCTGTATTGATAc                                                                                                                                    <  2:726271/141‑1 (MQ=11)
   cAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTAT‑TCTTTGCTGTATTGATAc                                                                                                                                    <  1:627324/141‑1 (MQ=11)
     gtgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTAT‑TCTTTGCTGTATTGATACtt                                                                                                                                  >  1:588571/1‑141 (MQ=17)
      tgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTAT‑TCTTTGCCGTATTGATACttt                                                                                                                                 >  1:662953/1‑141 (MQ=11)
      tgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTAT‑TCTTTGCCGTATTGATACttt                                                                                                                                 >  2:217202/1‑141 (MQ=255)
       gtTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTAT‑TCTTTGCTGTATTGATACTTTg                                                                                                                                <  1:234466/141‑1 (MQ=17)
       gtTCGCGTAACTTGCGTGCAAGATGCTAATTTCGTGGCATGGTGAAAATCCGTGGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTGTAAAGTAT‑TCTTTGCTGTATTGATACTTTg                                                                                                                                <  2:716900/141‑1 (MQ=11)
         tcgtttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTAT‑TCTTTGCTGTATTGATACTTTGAt                                                                                                                              >  1:136512/5‑141 (MQ=12)
             ttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTAT‑TCTTTGCTGTATTGATACTTTGATACCt                                                                                                                          <  2:81161/141‑1 (MQ=17)
                aCTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTAT‑TCTTTGCTGTATTGATACTTTGATACCTTGt                                                                                                                       <  2:671155/141‑1 (MQ=17)
                 cTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATACGTAGATCTGAAGAGACCTGCGGGTCTGTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGATA‑TCTTTGCTGTATTGTTACTTTGATACCTTGtc                                                                                                                      >  2:384145/1‑141 (MQ=11)
                  ttGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTCAATACATTCAATACCTTTTAAAGTAT‑TCTTTGCCGTATTGATACTGTGGTATCTTGtct                                                                                                                     >  1:87454/1‑141 (MQ=11)
                   tGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTAT‑TCTTTGCTGTATTGATACTTTGATACCTTGtctt                                                                                                                    <  1:433529/141‑1 (MQ=17)
                      tAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTAT‑TCTTTGCTGTATTGATACTTTGATACCTTGtctttct                                                                                                                 <  2:404203/141‑1 (MQ=17)
                        gCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTAT‑TCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTa                                                                                                               >  1:296025/1‑141 (MQ=17)
                         cATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTAT‑TCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTAc                                                                                                              >  1:723500/1‑141 (MQ=33)
                           tgatgCTAATTTCGTGGCATGGGGAGAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTAT‑TCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACtt                                                                                                            >  1:387062/1‑141 (MQ=11)
                           tgatgCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGCCCTACGGTTCTTTTTATATAGAGCGTAAATACATCCACTACCTTTTAAAGTAT‑TCTTTCCCGTATTGATACTTGCATTTCTTGTCTTTCTTCCtt                                                                                                            >  2:116820/1‑141 (MQ=11)
                                  aaTTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTAT‑TCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATg                                                                                                     >  1:57617/1‑141 (MQ=21)
                                   aTTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTAT‑TCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGa                                                                                                    <  1:255269/141‑1 (MQ=25)
                                    tttCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAATTACACTCAATATCTTTTAAAGTAT‑TCTTTGCTGTATTGATACCTTGATACCTTTTTTTTCTTACTTTAATTTgcc                                                                                                   >  2:203056/1‑139 (MQ=11)
                                     ttCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTAGATAGAGCGTAAATACACTCAATACCTTTTAAAGTAT‑TCTTTGCTCTATTGATCCTTTGATACCTGGTCTTTCTTACTTTAATATGACg                                                                                                  >  2:306351/1‑141 (MQ=11)
                                      tCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTAT‑TCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATTTGACgg                                                                                                 >  2:251451/1‑141 (MQ=255)
                                        gTGGCATGGCGAAAATCCGTAGATCCGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTAT‑TCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTg                                                                                               >  1:100074/1‑141 (MQ=255)
                                         tGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTAT‑TCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGa                                                                                              >  2:226192/1‑141 (MQ=255)
                                          ggCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTAT‑TCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAt                                                                                             >  1:250474/1‑141 (MQ=38)
                                                 cGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTAT‑TCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTc                                                                                      <  1:68455/141‑1 (MQ=255)
                                                 cGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTAT‑TCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTc                                                                                      >  1:379440/1‑141 (MQ=38)
                                                  gAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTAT‑TCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCa                                                                                     <  1:414081/141‑1 (MQ=255)
                                                    aaaTCCGTAGATCTGAAGATACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTAT‑TCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTGTAATATGACGGTGATCTTGCTCAAt                                                                                   <  2:7381/141‑1 (MQ=255)
                                                    aaaTCCGTAGAACTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTAT‑TCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAAt                                                                                   >  2:659305/1‑141 (MQ=255)
                                                     aaTCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAAAGAGCGTAAATACATTCAATACCTTTTAAAGTAT‑TCTTTGCCGTATTGATGCTTTGATATCTTGTCTTTATTACTTTAATATGACGGTGATCTTGATCAATg                                                                                  >  1:167046/1‑141 (MQ=11)
                                                        ccGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTAT‑TCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAgg                                                                               >  2:264137/1‑141 (MQ=255)
                                                          gTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTAT‑TCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGtt                                                                             <  1:761895/141‑1 (MQ=255)
                                                           tAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTAT‑TCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTa                                                                            >  1:77726/1‑141 (MQ=255)
                                                           tAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTAT‑TCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTa                                                                            >  1:149943/1‑141 (MQ=38)
                                                             gATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTAT‑TCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTAtt                                                                          <  2:610781/141‑1 (MQ=255)
                                                             gATCAGAAGAGACCTTCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTAT‑TCTTTGCTGTATTAATACTTTGATACCTGTTCTTTCGTAATATAATATGACGGTGATCTTGCTCAATGAGGTTAtt                                                                          <  2:162249/141‑1 (MQ=11)
                                                                 ggaatggaCCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTAT‑TCTTTCCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGa                                                                      <  2:569332/135‑1 (MQ=255)
                                                                   aaGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTAT‑TCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGatt                                                                    >  1:32634/1‑140 (MQ=37)
                                                                   aaGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTAT‑TCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGata                                                                    >  2:459513/1‑141 (MQ=37)
                                                                   aaGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTAT‑TCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGata                                                                    >  1:356354/1‑141 (MQ=38)
                                                                      agaCCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACATTTTAAAGTAT‑TCTTTGCTGTATTGATACTTTGATACCTTGTATTTCTTACTTTAATATGTCTGTGGTCTTGCTCAATGAGGTTATTCAGATAttt                                                                 >  2:419871/1‑141 (MQ=11)
                                                                      agaCCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTAT‑TCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATAttt                                                                 >  1:219001/1‑141 (MQ=255)
                                                                       gaCCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTAT‑TCTTTGCTGTGTTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCGATGAGGTTTTTCAGATGtttg                                                                >  1:550032/1‑140 (MQ=11)
                                                                       gaCCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTAT‑TCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATAtttg                                                                >  1:499232/1‑140 (MQ=255)
                                                                       gaCCTACGGTTCTTTTTATATAAAGCGTAAATACATTCAATACCTTTTAAAGTAT‑TCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTTATTTTGCTCACTGCGGTTACTCAGATATTTc                                                                >  2:27745/1‑141 (MQ=11)
                                                                        aCCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTAT‑TCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCg                                                               >  1:196203/1‑141 (MQ=255)
                                                                            gCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTAT‑TCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGt                                                           >  2:586482/1‑141 (MQ=255)
                                                                                 tCTTTTTATATAGAGCGTAAATACATGCAATACCTTTTAAAGTAT‑TCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAAt                                                      <  1:535340/141‑1 (MQ=255)
                                                                                 tCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTAT‑TCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAAt                                                      >  2:294650/1‑141 (MQ=38)
                                                                                    ttttATATAGGGCGTAAATACACTCAATACCTTTTAAAGTAT‑TCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATggc                                                   <  2:473290/141‑3 (MQ=255)
                                                                                       tatataGAGCGTAAATACATTCAATACCTTTTAAAGTAT‑TCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGt                                                <  1:44842/141‑1 (MQ=255)
                                                                                       tatataGAGCGTAAATACATTCAATACCTTTTAAAGTAT‑TCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGt                                                >  2:104568/1‑141 (MQ=255)
                                                                                       tatataGAGCGCAAAGTCTCTCAATACCTTTTAAAGTAT‑TCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACGTTAATATGACGGTGATCGTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGt                                                <  2:638338/141‑1 (MQ=14)
                                                                                         aataGAGCGTAAATACACTCAATACCTTTTAAAGTAT‑TCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCa                                              <  1:70332/140‑1 (MQ=255)
                                                                                           taGAGCGTAAATACACTCAATACGTTTT‑ACGTCTGGCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGGTCTTGCTCAATGAGGTTATTCAGGTATTTTGTTGTACAATGGCAGTCAgg                                            >  2:508064/1‑141 (MQ=14)
                                                                                             gagCGTAAATACATTCAATACCTTTTAAAGTAT‑TCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtt                                          >  2:491257/1‑141 (MQ=255)
                                                                                              ggCGTAAATATATTCAATACCTTTTAAAGTAT‑TCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgttt                                         <  2:135462/140‑1 (MQ=255)
                                                                                               gCGTAAATACACTCAATACCTTTTAAAGTAT‑TCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgttta                                        <  1:46956/141‑1 (MQ=255)
                                                                                                 gTAAATACATTCAATACCTTTTAAAGTAT‑TCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaag                                      <  2:536763/141‑1 (MQ=40)
                                                                                                  tAAATACACTCAATACCTTTTAAAGTAT‑TCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagt                                     <  2:333629/141‑1 (MQ=255)
                                                                                                   aaaTACATTCAATACCTTTTAAAGTAT‑TCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagtt                                    <  1:638939/141‑1 (MQ=255)
                                                                                                   aaaTACACTCAATACCTTTTAAAGTAT‑TCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagtt                                    <  2:748206/141‑1 (MQ=255)
                                                                                                    aaTACATTCAATACCTTTTAAAGTAT‑TCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagttt                                   <  1:314708/141‑1 (MQ=255)
                                                                                                    aaTACACTCAATACCTTTTAAAGTAT‑TCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagttt                                   <  2:178057/141‑1 (MQ=255)
                                                                                                    aaTACACTCAATACCTTTTAAAGTAT‑TCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagttt                                   >  1:178370/1‑141 (MQ=255)
                                                                                                     aTACACTCAATACCTTTTAAAGTAT‑TCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCACTGCGGTTATTCAGATATTTCGATGTACACTGGCAGTCAggtttaggttta                                  >  1:714478/1‑141 (MQ=14)
                                                                                                     aTACACTCAATACCTTTTAAAGTAT‑TCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagttta                                  >  1:459698/1‑141 (MQ=255)
                                                                                                     aTACACTCAATACCTTTTAAAGTAC‑TCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagttta                                  >  1:490130/1‑141 (MQ=255)
                                                                                                      tACACTCAATACCTTTTAAAGTAT‑TCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagtttaa                                 >  2:622464/1‑141 (MQ=255)
                                                                                                       acactCAATACCTTTTAAAGTAT‑TCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTaaa                                >  2:497873/5‑141 (MQ=255)
                                                                                                       acacTCAATACCTTTTAAAGTAT‑TCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTaaa                                <  2:709414/141‑1 (MQ=255)
                                                                                                         aatCAATACCTTTTAAAGTAT‑TCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTATAATGGCAGTCAGGTTTAAGTTTAAAAg                              <  2:188157/139‑1 (MQ=255)
                                                                                                          ttCAATACCTTTTAAAGTAT‑TCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGc                             <  1:55437/141‑1 (MQ=255)
                                                                                                          ctCAATACCTTTTAAAGTAT‑TCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGc                             <  1:653523/140‑1 (MQ=255)
                                                                                                             aaTACCTTTTAAAGTAT‑TCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTATAAGCttt                          >  1:516655/1‑141 (MQ=255)
                                                                                                             aaTACCTTTTAAAGTAT‑TCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACCGTCAGGTTTAAGTTTAACAGCttt                          >  2:363199/1‑141 (MQ=255)
                                                                                                                 ccTTTTAAAGTAT‑TCTTTGGTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAAtt                      >  2:354292/1‑141 (MQ=255)
                                                                                                                  cTTTTAATGTAT‑TCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTa                     <  2:147266/141‑1 (MQ=255)
                                                                                                                   ttttAAAGTAT‑TCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTAc                    <  2:431520/141‑1 (MQ=40)
                                                                                                                   ttttAAAGTAT‑TCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAAAATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTAc                    >  1:145989/1‑141 (MQ=255)
                                                                                                                   ttttAAAGTAT‑TCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCGGGTTTGTGTTTTAAAGCTTTAATTat                    >  1:335649/1‑140 (MQ=14)
                                                                                                                     ttAAAGTAT‑TCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACtt                  <  1:306295/141‑1 (MQ=255)
                                                                                                                       aaaGTAT‑TCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTa                >  1:342712/1‑141 (MQ=255)
                                                                                                                       aaaGTAT‑TCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTACTTATTTTa                >  2:446406/1‑141 (MQ=255)
                                                                                                                             t‑tCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCAtt          <  1:207613/141‑1 (MQ=255)
                                                                                                                             t‑tCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCAtt          <  1:126526/141‑1 (MQ=255)
                                                                                                                               tCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTg         >  1:403467/1‑141 (MQ=255)
                                                                                                                               tCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGGTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTg         >  2:530758/1‑141 (MQ=255)
                                                                                                                                ttttGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGc        <  1:116026/140‑1 (MQ=255)
                                                                                                                                cTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGc        <  2:652472/141‑1 (MQ=255)
                                                                                                                                cTTTGCCTTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGc        <  1:266376/141‑1 (MQ=255)
                                                                                                                                 tttGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCt       >  2:685884/1‑141 (MQ=255)
                                                                                                                                 tttGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCt       <  1:607489/141‑1 (MQ=255)
                                                                                                                                  ttGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTTTTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTACTTACTTTAACCATTGCtt      >  1:281211/1‑140 (MQ=255)
                                                                                                                                     cTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGTTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCt   <  2:555670/141‑1 (MQ=255)
                                                                                                                                      tGTATTGATACTTTGATACCTTGTCTTTCTTACTTGAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCtt  <  1:243399/141‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                            
TGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTAT‑TCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTT  >  CP000730/35864‑36137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑