BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	36,378	C→T	L58L (TTG→TTA)	tpn ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,378	0	C	T	93.6%	820.0 / 40.1	377	L58L (TTG→TTA)	tpn	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base C (7/17); new base T (171/179); total (180/197)
Fisher's exact test for biased strand distribution p-value = 8.94e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.65e-01

Only 100 of 377 total aligned reads displayed.

TGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAA  >  CP000730/36232‑36514

tccTTACGCAACTAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTTATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATaa                                                                                                                                        <  1:59412/147‑1 (MQ=11)

  ctcactcAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATaaaa                                                                                                                                      <  1:171955/143‑1 (MQ=11)

    tACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATTAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAAtt                                                                                                                                    <  1:24700/149‑1 (MQ=255)

     aCGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTg                                                                                                                                   <  1:324710/149‑1 (MQ=37)

      cGCAACCAAATATCTAATGTTTGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCCTCAATACGCCATTTGTAATAAGCTTGTTTATGCATTTTCTTCCAAATTTGATATAAAATTgg                                                                                                                                  <  1:387258/149‑1 (MQ=11)

      cGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTTTTATACGTCTCATCAATACACCATTTGTAATAAGCTTTTTTATACTTTTTCTTCCAAATTTTGTATCAAATTgg                                                                                                                                  >  1:75067/1‑149 (MQ=11)

      cGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTgg                                                                                                                                  <  1:361383/149‑1 (MQ=255)

      cGCAACCAAATATCTAATGTATGTCCCTCTGCCTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCACCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTgg                                                                                                                                  <  1:400207/149‑1 (MQ=255)

       gCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTTTTTTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATTCTTTTTCTTCCAGATTTGTTATAAAATTggg                                                                                                                                 >  1:237776/1‑149 (MQ=11)

       gCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTTATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTggg                                                                                                                                 >  1:378200/1‑149 (MQ=255)

       gCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGTTATACGTCTCATCAATACGCCATTTGTAATATGCTTTTTTATGCTTTTTCTTCTTGATTTAATATAAATTTggg                                                                                                                                 >  1:9057/1‑149 (MQ=11)

       gCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTggg                                                                                                                                 <  1:424740/149‑1 (MQ=255)

        cAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTgggg                                                                                                                                <  1:21616/149‑1 (MQ=37)

               atatCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATGTAAAATTGGGGCGTATTc                                                                                                                         >  1:214433/1‑149 (MQ=11)

                tatCTAATGTATGTCCCTCTGTATCAATGGCACGGTATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAAGACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCt                                                                                                                        <  1:118380/149‑1 (MQ=11)

                tatCGAAGGTAGGTCGCTCTGCATCAATGGCAAGATATAAATAGCTCCATTTTCCTTTTATTTTTATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCt                                                                                                                        <  1:128754/149‑1 (MQ=11)

                  tCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATGTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTg                                                                                                                      <  1:312637/149‑1 (MQ=255)

                     aaTGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAAc                                                                                                                   <  1:48144/149‑1 (MQ=255)

                      atgtatgtCCCTCTGTATCAATGGCACGATTGTAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGTAAATAGATTGGGGCGTATTCTTGAAcc                                                                                                                  >  1:441356/1‑149 (MQ=14)

                       agtatgtCCCTCTGCATCAATGGCCCGATATAAATAGCTCCACTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAAccc                                                                                                                 <  1:461583/148‑1 (MQ=255)

                        gtatgtCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCa                                                                                                                <  1:211359/149‑1 (MQ=25)

                         tatgtCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGAGATAAAATTGGGGCATATTCTTGAACCCaa                                                                                                               >  1:139936/1‑149 (MQ=255)

                            ttCCCTCTTCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACgg                                                                                                            <  1:121069/148‑1 (MQ=255)

                              cccTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATCTACAATTGGGGCATGTTCTTGAACCCAACGGTa                                                                                                          >  1:350302/1‑149 (MQ=11)

                                ctctGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATGCTTGAACCCAACGTTAGa                                                                                                        >  1:523004/1‑149 (MQ=11)

                                    gCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGt                                                                                                    <  1:598411/149‑1 (MQ=40)

                                     taTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGtt                                                                                                   <  1:245115/148‑1 (MQ=255)

                                     taTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGtt                                                                                                   >  1:276815/2‑149 (MQ=255)

                                      ataaaTGGCACGATATAAATAGCTCCATTTTCCTTTTATTCTGATGTACGTCTCATCAATACGCCATTTGTAATAATCTGTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTTGGGCATATTCTTGAACCCAACGGTAGACCGTTg                                                                                                  <  1:198250/146‑1 (MQ=11)

                                      aTCAAAGGCACGACATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTg                                                                                                  <  1:57928/149‑1 (MQ=255)

                                       tCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGa                                                                                                 <  1:321569/149‑1 (MQ=21)

                                       acAATGGCACGATATAAATAGCTTCATTTTCCTGTTTTTTTAGTATACGTCTCATCAGTACTCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGa                                                                                                 <  1:562291/148‑1 (MQ=11)

                                        cAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTACAAATTTGATATAAAATTGGGACATCTTCTTGAACCAAACGGTAGACCGTTGaa                                                                                                >  1:572961/1‑149 (MQ=11)

                                        cAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGGTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGaa                                                                                                >  1:177338/1‑149 (MQ=255)

                                        cAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGaa                                                                                                >  1:469832/1‑149 (MQ=255)

                                         aaTGGCACTATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAat                                                                                               <  1:353416/149‑1 (MQ=255)

                                          aTGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAatg                                                                                              >  1:329176/1‑149 (MQ=255)

                                             gCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAatgatg                                                                                           <  1:231652/149‑1 (MQ=21)

                                              cACTATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAAtgatga                                                                                          <  1:485987/149‑1 (MQ=21)

                                              cACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCACTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCATATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAAtgatga                                                                                          >  1:472746/1‑149 (MQ=11)

                                               aCGATATAATTAGCTCCATTTTGTTTTTATTTTGATGTACGTCTCACCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGATCCCAACGGTAGACCGTTGAATGATGaa                                                                                         <  1:3160/149‑1 (MQ=11)

                                               aCGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGaa                                                                                         <  1:332134/149‑1 (MQ=18)

                                                  atatAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGt                                                                                      >  1:186948/1‑149 (MQ=21)

                                                      aaaTAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTATATCGTTGAATGATGAACGTTTac                                                                                  >  1:156117/1‑149 (MQ=255)

                                                       aaTAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTaca                                                                                 >  1:53896/1‑149 (MQ=21)

                                                           gCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAACTTTGCTACTTTGCTTAAACCCAACGGTGGACCGTAGAATGATGAACGTTTAcaccac                                                                             >  1:432729/1‑149 (MQ=11)

                                                           gCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcaccac                                                                             >  1:382031/1‑149 (MQ=255)

                                                           gCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCAGATTCTTGAACCCAACGGTAGATCGTTGAATGCTGTACGTTTAaaacac                                                                             >  1:101498/1‑149 (MQ=11)

                                                            cTCCATTTTCCTATTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACTGTAGATCGTTGAATGATGAACGTTTACACCACg                                                                            >  1:406842/1‑149 (MQ=11)

                                                             tCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTGCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACCCCACGt                                                                           >  1:174099/1‑149 (MQ=11)

                                                             tCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGt                                                                           >  1:195121/1‑149 (MQ=255)

                                                             tCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGt                                                                           >  1:569108/1‑149 (MQ=255)

                                                             tCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGt                                                                           >  1:145557/1‑149 (MQ=255)

                                                              ccATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAAACCACGtc                                                                          >  1:503127/1‑148 (MQ=11)

                                                                cttttCCTTGTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTct                                                                        <  1:36338/148‑2 (MQ=11)

                                                                aTTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTcc                                                                        <  1:340470/149‑1 (MQ=21)

                                                                 ttttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTctc                                                                       <  1:307460/149‑3 (MQ=255)

                                                                  tttCCTTTTATTTTGATTTACGTCTCATCAATACGCCATTTTGAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCt                                                                      <  1:493067/149‑1 (MQ=255)

                                                                  tttCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCt                                                                      <  1:448917/149‑1 (MQ=18)

                                                                  tttCCTTCTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCt                                                                      <  1:207258/149‑1 (MQ=255)

                                                                    tCCTTTTTTTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTa                                                                    <  1:322659/149‑1 (MQ=11)

                                                                    tCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTa                                                                    <  1:396519/149‑1 (MQ=255)

                                                                     ccTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTaa                                                                   <  1:108455/149‑1 (MQ=255)

                                                                       ttttATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAata                                                                 <  1:421710/149‑1 (MQ=11)

                                                                        tttATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAATCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGAGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAatat                                                                <  1:553922/149‑1 (MQ=255)

                                                                         ttATTTTGATATACGGCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTACAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATAtt                                                               <  1:126978/149‑1 (MQ=11)

                                                                          tATTTTGATGTCTGTATCATCCATCTGCCCTTTGTAATAAGCTTTATTATTCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGTACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATAttt                                                              <  1:124552/149‑1 (MQ=11)

                                                                           ttgttGATTTACGTCTCAGCAATACGCCATTTGTAAGAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTc                                                             <  1:270878/146‑1 (MQ=11)

                                                                           aTTTTGATGTACGTCTCATCAACACGCCATTTGTAATAAGCTTTCTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTc                                                             <  1:66011/149‑1 (MQ=12)

                                                                           aTTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTc                                                             <  1:291533/149‑1 (MQ=11)

                                                                               tGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGat                                                         <  1:465948/149‑1 (MQ=11)

                                                                                tatatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTGTTTCTTCCAAATTTGCTCTAACCATTGTTCATATTCTTCGACCTAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGata                                                        <  1:438044/145‑1 (MQ=11)

                                                                                gatatccggctcATCAATCCGACCTTTGTAATAAGCTTTCTTATGCTTTTTCTTCCAAATTCGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGata                                                        <  1:104058/140‑1 (MQ=11)

                                                                                gatatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGata                                                        >  1:417412/5‑149 (MQ=255)

                                                                                 aTGTACGTCTCATCAATACGGCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGatat                                                       <  1:544335/149‑1 (MQ=21)

                                                                                    tACGTTTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATc                                                    <  1:94670/149‑1 (MQ=21)

                                                                                     aCGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCCCGTTCTCTTAATATTTCAGATATATCa                                                   >  1:161429/1‑149 (MQ=255)

                                                                                      cGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCAc                                                  >  1:518928/1‑149 (MQ=255)

                                                                                       gTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCAAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTATTAATATTTCAGATATATCACg                                                 >  1:342985/1‑149 (MQ=11)

                                                                                        tctcATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGa                                                >  1:37439/1‑149 (MQ=255)

                                                                                          tcatcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATCTAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTGTAATGATCAACGGTTACACCACGTGCTCTTAATATTTCAGATATATCGCGATa                                              >  1:221387/1‑149 (MQ=11)

                                                                                           catcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATag                                             >  1:513017/1‑148 (MQ=255)

                                                                                           catcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATaa                                             <  1:456213/149‑1 (MQ=21)

                                                                                             tcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGCATGATGCACGCTTACACCACGTTCCCTTAATATTTCAGATATCTCACGCTACCt                                           >  1:529059/1‑149 (MQ=11)

                                                                                               aaTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCa                                         >  1:336290/1‑149 (MQ=11)

                                                                                               aaTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGCATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCa                                         >  1:294424/1‑149 (MQ=21)

                                                                                                atccGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCaa                                        <  1:253779/146‑1 (MQ=11)

                                                                                                 tACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAAt                                       <  1:564571/149‑1 (MQ=11)

                                                                                                  aCGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATg                                      <  1:536427/149‑1 (MQ=255)

                                                                                                     ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATGcat                                   >  1:81516/1‑149 (MQ=11)

                                                                                                     ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGcat                                   >  1:261462/1‑149 (MQ=17)

                                                                                                      cATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATGcata                                  <  1:298612/149‑1 (MQ=11)

                                                                                                      cATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGcata                                  <  1:61369/149‑1 (MQ=14)

                                                                                                           ttaataaGCTTTTTTATGCTTTTTCTTTCAAATTTGGTACAAAATTGGGGCATATTCTTGAACCCTACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTa                             <  1:285887/148‑1 (MQ=255)

                                                                                                            taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAACATTGGGGCATATTCTTGAACTCAACGGTTGATCGTTGAATGATGAACGTTTACACCTCGTTCTCTTATTATTTCGGATATATCACGATAGCTCAATGCATATCTTAg                            <  1:8521/149‑1 (MQ=11)

                                                                                                            taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATGCATATCTTAg                            <  1:389084/149‑1 (MQ=11)

                                                                                                            taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATGCATATCTTAg                            >  1:478549/1‑149 (MQ=11)

                                                                                                            taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGCATATCTTAg                            <  1:201947/149‑1 (MQ=14)

                                                                                                                              tttttCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTAc          >  1:497928/1‑149 (MQ=35)

                                                                                                                                      ccAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATaa  <  1:427364/149‑1 (MQ=35)

TGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAA  >  CP000730/36232‑36514

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`