Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 832,706 A→T 5.7% I488F (ATT→TTT)  secA1 → Sec family Type I general secretory pathway preprotein translocase subunit SecA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730832,7060AT 5.7% 205.4 / 4.1 73I488F (ATT→TTT) secA1Sec family Type I general secretory pathway preprotein translocase subunit SecA
Reads supporting (aligned to +/- strand):  ref base A (29/39);  new base T (2/2);  total (31/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.04e-01

ACTGTTGCAGTTGAGACTTCTGAATATATTTCAAATTTACTTAAAAAACGTGGTATCCGTCATGATGTGTTAAATGCGAAAAATCATGAACGTGAAGCTGAAATTGTTGCAGGCGCTGGACAAAAAGGTGCCGTTACTATTGCCACTAACATGGCTGGTCGTGGTACAGATATCAAATTAGGTGAAGGCGTAGAGGAATTAGGCGGTTTAGCAGTAATAGGTACAGAGCGACATGAATCTCGTCGTATTGATGACCAGTTACGTGGTCGTTCTGGAC  >  CP000730/832568‑832844
                                                                                                                                          |                                                                                                                                          
aCTGTTGCAGTTGAGACTTCGGAATATATTTCAAATTTACGTAAAAAACGTGGTATCCGTCATGATGTGTTAAATGCGAAAAATCATGAACGTGAAGCTGAAATTGTTGCAGGCGCTGGACAAAAAGGTGCCGTTACTAtt                                                                                                                                          <  1:61819/141‑1 (MQ=255)
aCTGGTGCACTTGAGACTTTCGAATATATTTAAAATTTACTTAAAAAACGTTGTATCCGTCAGGATGTGTTAAATGCGAAAAATCAGGAACGTTAAGCGGAAATTGTTGCAGGCGCTGGACAAAAAGGTGCCGTTACTAtt                                                                                                                                          <  2:173835/141‑1 (MQ=255)
  tGTTGCAGTTGAGACTTCTGAATATATTTCAAATTTACTTAAAAAACGTGGTATCCGTCATGATGTGTTAAATGCGAAAAATCATGAACGTGAAGCTGAAATTGTTGCAGGCGCTGGACAAAAAGGTGCCGTTACTATTGc                                                                                                                                        >  1:300479/1‑141 (MQ=255)
   gTTGCAGTTGAGACTTCTGAATATATTTCAAATTTACTTAAAAAACGTGGTATCCGTCATGATGTGTTAAATGCGAAAAATCATGAACGTGAAGCTGAAATTGTTGCAGGCGCTGGACAAAAAGGTGCCGTTACTATTGcc                                                                                                                                       >  1:184794/1‑141 (MQ=255)
     tGCAGTTGAGACTTCTGAATATATTTCAAATTTACTTAAAAAACGTGGTATCCGTCATGATGTGTTAAATGCGAAAAATCATGAACGTGAAGCTGAAATTGTTGCAGGCGCTGGACAAAAAGGTGCCGTTACTATTGCCAc                                                                                                                                     >  2:503797/1‑141 (MQ=255)
      gCAGTTGAGACTTCTGAATAGATTTCAAATTTACTTAAAAAACGTGGTGTCCGTCATGATGTGGTAAATGCGAAAAATCATGAACGTGAAGCTGAGATTGTTGCAGGCGCTGGTCAAAAAGGTGCCGTTACTATTGCCAcg                                                                                                                                    >  1:429039/1‑140 (MQ=255)
        aGTTGAGACTTCTGAATATATTTCAAATTTACTTAAAAAACGTGGTATCCGTCATGATGTGTTAAATGCGAAAAATCATGAACGTGAAGCTGAAATTGTTGCAGGCGCTGGACAAAAAGGTGCCGTTACTATTGCCACTaa                                                                                                                                  <  1:2784/141‑1 (MQ=255)
         gTTGAGACTTCTGAATATATTTCAAATTTACTTAAAAAACGTGGTATCCGTCATGATGTGTTAAATGCGAAAAATCATGAACGTGAAGCTGAAATTGTTGCAGAAGAAAAAAAAAAAGGTGCCGTTACTATTGCCACTAAc                                                                                                                                 <  2:318507/141‑1 (MQ=255)
             agaCTTCTGAATATATTTCAAATTTACTTAAAAAACGTGGTATCCGTCATGATGTGTTAAATGCGAAAAATCATGAACGTGAAGCTGAAATTGTTGCAGGCGCTGGACAAAAAGGTGCCGTTACTATTGCCACTAACATgg                                                                                                                             >  1:123895/1‑141 (MQ=255)
             agaCTTCTGAATATATTTCAAATTTACTTAAAAAACGTGGTATCCGTCATGATGTGTTAAATGCGAAAAATCATGAACGTGAAGCTGAAATTGTTGCAGGCGCTGGACAAAAAGGTGCCGTTACTATTGCCACTAACATgg                                                                                                                             <  2:482897/141‑1 (MQ=255)
                   tcgAATATATTGCAAATTTAATTAAAAAACGGGGTATCCGTCATGATGTGTTAAATGCGAAAAATCATGAACGTGAAGCTGAAATTGTTGCAGGAAATAGAAAAAAAGGTGCCGTTACTATTGCCACTAACATGGCTGGTc                                                                                                                       <  2:266797/139‑1 (MQ=255)
                   cTGAATATATTTCAAATTTACTTAAAAAACGTGGTATCCGTCATGATGTGTTAAATGCGAAAAATCATGAACGTGAAGCTGAAATTGTTGCAGGCGCTGGACAAAAAGGTGCCGTTACTATTGCCACTAACATGGCTGGTc                                                                                                                       <  1:100060/141‑1 (MQ=255)
                        ttgatTTCAAATGTGCTTAAAAATGGTGGGATCCGTCATTATGTGTTCAATGCGTAAAATCATGAACGTGAAGCTGTTATTGTTGCAGAAGAAGAAAAAAAAGGTGCCGTTACTATTGCCACTAACATGGCTGGTCGTGGt                                                                                                                  <  2:198500/138‑1 (MQ=255)
                        tatatTTCAAATTTACTTAAAAAACGTGGTATCCGTCATGATGTGTTAAATGCGAAAAATCATGAACGTGAAGCTGAAATTGTTGCAGGCGCTGGACAAAAAGGTGCCGTTACTTTTGCCACTAACATGGCTGGTCGTGGt                                                                                                                  >  2:240866/1‑141 (MQ=255)
                        tatatTTCAAATTTACTTAAAAAACGTGGTATCCGTCATGATGTGTTAAATGCGAAAAATCATGAACGTGAAGCTGAAATTGTTGCAGGCGCTGGACAAAAAGGTGCCGTTACTTTTGCCACTAACATGGCTGGTCGTGGt                                                                                                                  >  2:240775/1‑141 (MQ=255)
                          tatTTCAAATTTACTTAAAAAACGTGGTATCCGTCATGATGTGTTAAATGCGAAAAATCATGAACGTGAAGCTGAAATTGTTGCAGGCGCTGGACAAAAAGGTGCCGTTACTATTGCCACTAACATGGCTGGTCGTGGTAc                                                                                                                >  2:104765/1‑141 (MQ=255)
                          tatTTCAAATTTACTTAAAAAACGTGGTATCCGTCATGATGTGTTAAATGCGAAAAATCATGAACGTGAAGCTGAAATTGTTGCAGGCGCTGGACAAAAAGGTGCCGTTACTATTGCCACTAACATGGCTGGTCGTGGTAc                                                                                                                <  1:379342/141‑1 (MQ=255)
                              tCAAATTTACTTAAAAAACGTGGTATCCGTCATGATGTGTTAAATGCGAAAAATCATGAACGTGAAGCTGAAATTGTTGCAGGCGCTGGACAAAAAGGTGCCGTTACTATTGCCCCTCAACCAGCTGGTCGTGGTACatag                                                                                                            <  2:510635/141‑4 (MQ=255)
                                 aaTTTACTTAAAAAACGTGGTATCCGTCATGATGTGTTAAATGCGAAAAATCATGAACGTGAAGCTGAAATTGTTGCAGGCGCTGGACAAAAAGGTGCCGTTACTATTGCCACTAACATGGCTGGTCGTGGTACAGATATc                                                                                                         >  1:184593/1‑141 (MQ=255)
                                 aaTTTACTTAAAAAACGTGGGAGCCGTCATGATGTGTTAAATGCGAAAAATCATGAACGTGAAGCTGAAATTGTTGCAGGCGCTGGACAAAAAGGTGCCGTTACTATTGCCACTAACATGGCTGGTCGTGGTACAGATATc                                                                                                         <  1:111771/141‑1 (MQ=255)
                                  aTTTACTTAAAAAACGTGGTATCCGTCATGATGTGTTAAATGCGAAAAATCATGAACGTGAAGCTGAAATTGTTGCAGGCGCTGGACAAAAAGGTGCCGTTACTATTGCCACTAACATGGCTGGTCGTGGTACAGATATCa                                                                                                        >  1:606408/1‑141 (MQ=255)
                                  aTTTACTTAAAAAACGTGGTATCCGTCATGATGTGTTAAATGCGAAAAATCATGAACGTGAAGCTGAAATTGTTGCAGGCGCTGGACAAAAAGGTGCCGTTACTATTGCCACTAACATGGCTGGTCGTGGTACAGATATCa                                                                                                        >  1:32003/1‑141 (MQ=255)
                                   tttACTTAAAAAACGTGGTATCCGTCATGATGTGTTAAATGCGAAAAATCATGAACGTGAAGCTGAAATTGTTGCAGGCGCTGGACAAGAAAGTGCCGTTACTATTGCCACTAACATGGCTGGTCGTGGGACAAATATCaa                                                                                                       >  1:80856/1‑141 (MQ=255)
                                     tACTTAAAAAACGTGGTATCCGTCATGATGTGTTAAATGCGAAAAATCATGAACGTGAAGCTGAAATTGTTGCAGGCGCTGGACAAAAAGGTGCCGTTACTATTGCCACTAACATGGCTGGTCGTGGTACAGATATCAAAt                                                                                                     <  1:104765/141‑1 (MQ=255)
                                     tACTTAAAAAACGTGGTATCCGTCATGATGTGTTAAATGCGAAAAATCATGAACGTGAAGCTGAAATTGTTGCAGGCGCTGGACAAAAAGGTGCCGTTACTATTGCCACTAACATGGCTGGTCGTGGTACAGATATCAAAt                                                                                                     >  1:267609/1‑141 (MQ=255)
                                      aCTTAAAAAACGTGGTATCCGTCATGATGTGTTAAATGCGAAAAATCATGAACGTGAAGCTGAAATTGTTGCAGGCGCTGGACAAAAAGGTGCCGTTACTATTGCCACTAACATGGCTGGTCGTGGTACGGATATCAAAtt                                                                                                    >  1:134628/1‑141 (MQ=255)
                                       cTTAAAAAACGTGGTATCCGTCATGATGTGTTAAATGCGAAAAATCATGAACGTGAAGCTGAAATTGTTGCAGGCGCTGGACAAAAAGGTGCCGTTACTATTGCCACTAACATGGCTGGTCGTGGTACAGATATCAAATTa                                                                                                   <  2:351818/141‑1 (MQ=255)
                                         tAAAAAACGTGGTATCCGTCATGATGTGTTAAATGCGAAAAATCATGAACGTGAAGCTGAAATTGTTGCAGGCGCTGGACAAAAAGGTGCCGTTACTATTGCCACTAACATGGCTGGTCGTGGTACAGATATCAAATTAgg                                                                                                 <  1:85439/141‑1 (MQ=255)
                                         tAAAAAACGTGGTATCCGTCATGATGTGTTAAATGCGAAAAATCATGAACGTGAAGCTGAAATTGTTGCAGGCGCTGGACAAAAAGGTGCCGTTACTATTGCCACTAACATGGCTGGTCGTGGTACAGATATCAAATTAgg                                                                                                 <  1:503797/141‑1 (MQ=255)
                                            aaaaCGTGGTAACCGTTATGATGTGTTAAATGCGGAAAACCATTGACTAGAAACGTTAATTGTAGCAAGAAATAAAAAAAAAGGTGCCGTTACTATTGCCACTAACATGGCTGGTCGTGGTACAGATATCAAATTAGGTGa                                                                                              <  2:218076/141‑1 (MQ=255)
                                              aaCGTGGTATCCGTCATGATGTGTTGAATGCGAAAAATCATGAACGTGAAGCTGAAATTGTTGCAGGCGCTGGACAAAAAGGTGCCGTTACTATTGCCACTAACATGGCTGGTCGTGGTACAGATATCAAATTAGGTGAAg                                                                                            <  1:249378/141‑1 (MQ=255)
                                               aCGTGGTATCCGTCATGATGTGTTAAATGCGAAAAATCATGAACGTGAAGCTGAAATTGTTGCAGGCGCTGGACAAAAAGGTGCCGTTACTATTGCCACTAACATGGCTGGTCGTGGTACAGATATCAAATTAGGTGAAgg                                                                                           <  1:460887/141‑1 (MQ=255)
                                                    gTAGCCGCCATGATGAAAGAAATGCGAAAAAGCATAAACGTGAATCTGCAATTGTTGCAGGCGCTGGACAAAAAGGTACCGTTACTATTGCCACTAACATGGCTGGTCGTGGTACGGATATCAAATAAGGTAAAGGCGTag                                                                                      >  2:20923/1‑141 (MQ=255)
                                                      aTCCGTCATGATGTGTTAAATGCGAAAAATCATGAACGTGAGGCTGGAATTGTTGCAGGCGCTGGACAAAAAGGTGCCGTTACTATTGCCACTAACATGGCTGGTCGTGGTACAGATATCAAATGGGGTGAAGGCGTagag                                                                                    >  1:418575/1‑141 (MQ=255)
                                                      aTCCGGCATGATGTGTTAAATGCGAAAAATCATGAACGTGAAGCTGAAATTGTTGCAGGCGCTGGACAAAAAGGTGCCGTTACTATTGCCACTAACATGGCTGGTCGTGGTACAGATATCAAATTAGGTGAAGGCGTagag                                                                                    <  1:15249/141‑1 (MQ=255)
                                                        ccGTCATGATGTGTTAAATGCGAAAAATCATGAACGTGAAGCTGAAATTGTTGCAGGCGCTGGACAAAAAGGTGCCGTTACTATTGCCACTAACATGGCTGGTCGTGGTACAGATATCAAATTAGGTGAAGGCGTAGAGGa                                                                                  <  2:596482/141‑1 (MQ=255)
                                                          gTCTTGATGTGTTAGATGCGAAAAATCATGGACGTGAATCCGAAAATGTTGCTGGCGCTGGACAAAAAGGTGCCGTTACTATTGCCACTAACATGGCTGGTCGTGGTACAGATATCAAATTAGGTGAAGGCGTAGAGGCAt                                                                                <  2:429039/141‑1 (MQ=255)
                                                          gTCATGATGTGTTAAATGCGAAAAATCATGAACGTGAAGCTGAAATTGTTGCAGGCGCTGGACAAAAAGGTGCCGTTACTATTGCCACTAACATGGCTGGTCGTGGTACAGATATCAAATTAGGTGAAGGCGTAGAGGAAt                                                                                <  2:513773/141‑1 (MQ=255)
                                                           tcctgatgTGTTAAATGAGAAAAATCATGAACGTGAAGCTGAAATTGTTGCAGGCGCCGGACAAAAAGGTGCCGTTACTATTGCCACTAACATGGCTGGTCGTTGTACAGATATCAAATTAGGTGAAGGCGTAGAGGAAtt                                                                               <  2:247799/138‑1 (MQ=255)
                                                            cATGATGTGTTAAATGCGAAAAATCATGAACGTGAAGCTGAAATTGTTGCAGGCGCTGGACAAAAAGGTGCCGTTACTATTGCCACTAACATGGCTGGTCGTGGTACAGATATCAAATTAGGTGAAGGCGTAGAGGAATTa                                                                              <  1:439146/141‑1 (MQ=255)
                                                             atgcgtggtTTAATGCGAAAAATCATGAACGGTTAGCTGAAATTGTTGCAGGCGCGGTTGAAAAAGTTGCCGTTACTATTGCCACTCACATGGCTGGGCGTGGTACAGATATCAAATTAGGTGAAGGCGTAGAGGAATTAg                                                                             <  2:473321/134‑1 (MQ=255)
                                                               gatgTGTTAAATGCGAAAAATCATGGACGTGAAGCTGAAATTGTTGCAGGCGCTGGACAAAAAGGTGCCGTTACTATTGCCACTAACATGGCTGGTCGTGGTACAGATATCAAATTAGGTGAAGGCGTAGAGGAATTAGGc                                                                           <  1:70089/141‑1 (MQ=255)
                                                               gatgTGTTAAATGCGAAAAATCATGAACGTGAAGCTGAAATTGTTGCAGGCGCTGGACAAAAAGGTGCCGTTACTATTGCCACTAACATGGCTGGTCGTGGTACAGATATCAAATTAGGTGAAGGCGTAGAGGAATTAGGc                                                                           <  1:345611/141‑1 (MQ=255)
                                                                tgatgtTAACTGCGGAAAAGCATGAAGGGGAGGGTGAAAGTGTTGCAGGCGCTGGACAAAAAGGTGCCGTTACTATTGCCACTAACATGGCTGGTCGTGGTACAGATATCAAATTAGGTGAAGGCGTAGAGGAATTAGGCg                                                                          <  2:573777/138‑1 (MQ=255)
                                                                  gtgtTAAATGCGAAAAATCATGAACGTGAAGCTGAAAGTGTTGCAGGCGCTGGACAAAAAGGTGCCGTTACTATTGCCACTAACATGGCTGGTCGTGGTACATATATCAAATTAGGTGAAGGCGTAGAGGAATTAGGCGGt                                                                        <  1:420460/141‑1 (MQ=255)
                                                                     ttAAATGCGAAAACTCATGAACGTGAAGCTGAAATTGTTGCAGGCGCTGGACAAAAAGGTGCCGTTACTATTGCCACTAACATGGCTGGTCGTGGTACCGATATCAAATTAGGTGGAGGCGTGCAGGAATCAGGCGGttgg                                                                     >  2:573956/1‑139 (MQ=255)
                                                                       aactGCGAAAAATCATGAACGCGAAGCTGAAATTGTTGCAGGCGCTGGACAAAAAGGTGCCGTTACTATTGCCACTCACATGGCTGGTCGTGGTACAGATATCAAATTAGGTGAAGGCGTAGAGGAATTAGGCGGTTTAGc                                                                   <  1:524141/138‑1 (MQ=255)
                                                                                 aaTCATGAACGTGAAGCTGAAATTGTTGCAGGCGCTGGACAAAAAGGTGCCGTTACTATTGCCACTAACATGGCTGGTCGTGGTCCAGATATCAAATTAGGTGAAGGCGGAGAGGAATTAGGCGGTTTAGCAGTAATAGGt                                                         >  1:53508/1‑141 (MQ=255)
                                                                                      tGAACGTGAAGCTGAAATTGTTGCAGGCGCTGGACAAAAAGGTGCCGTTACTATTGCCACTAACATGGCTGGTCGTGGTACAGATATCAAATTAGGTGAAGGCGTAGAGGAATTAGGCGGTTTAGCAGTAATAGGTACagg                                                    >  2:24369/1‑140 (MQ=255)
                                                                                       gAACGTGAAGCTGAAATTGTTGCAGGCGCTGGACAAAAAGGTGCCGTTACTATTGCCACTAACATGGCTGGTCGTGGTACAGATATCAAATTAGGTGAAGGCGTAGAGGAATTAGGCGGTTTAGCAGTAATAGGTACagag                                                   >  2:441400/1‑141 (MQ=255)
                                                                                           ttGAAGCTGAAATTGTTGCAGGCGCTGGACAAAAAGGTGCCGTTACTATTGCCACTAACATGGCTGGTCGTGGTACAGATATCAAATTAGGTGAAGGCGTAGAGGAATTAGGCGGTTTAGCAGTAATAGGTACAGAGCGAc                                               <  2:25509/140‑1 (MQ=255)
                                                                                            tGAAGCTGAAATTGTTGCAGGCGCTGGACAAAAAGGTGCCGTTACTATTGCCACTAACATGGCTGGTCGTGGTACAGATATCAAATTAGGTGAAGGCGTAGAGGAATTAGGCGGTTTAGCAGTAATAGGTACAGAGCGACa                                              >  1:379887/1‑141 (MQ=255)
                                                                                                gCTGAAATTCGTGCAGGCGCTGGAGAAAAAGGTGCCGTTACTATTGCCCCTAACATGGCTGGTCGTGGTACAGATATCAAATTAGGTGAAGGCGTAGAGGAATTAGGCGGTTTAGCAGTAATAGGTACAGAGCGACAGGaa                                          >  1:447653/1‑141 (MQ=255)
                                                                                                   gAAATTGTTGCAGGCGCTGGACAAAAAGGTGCCGTTACTATTGCCACTAACATGGCTGGTCGTGGTACAGATATCAAATTAGGTGAAGGCGTAGAGGAATTAGGCGGTTTAGCAGTAATAGGTACAGAGCGACATGAAtcc                                       >  1:197623/1‑140 (MQ=255)
                                                                                                    aaaTTGTTGCAGGCGCTGGACAAAAAGGTGCCGTTACTATTGCCACTAACATGGCTGGTCGTGGTACAGATATCAAATTAGGTGAAGGCGTAGAGGGAGTCGGCGGTTTAGCAGTAGTACGTAAAGAGCGACATGAAtctc                                      >  2:59245/1‑141 (MQ=255)
                                                                                                      agtgttgCAGGCGCTGGACAAAAAGGTGCCGTTACTATTGCCACTAACATGGCTGGTCGTGGTACAGATATCAAATTAGGTGAAGGCGTAGAGGCATTAGGCTGTTTAGCAGTAATAGGTACAGAGCGACATGAATCtcgt                                    <  2:467847/139‑1 (MQ=255)
                                                                                                      agtggtgCCGGCGCGGGGCAAAGAGGTGCCGTTACTGTTGCGACTAACATGGCTGGTCGGGGTACAGGTATCAAATTAGGAGAAGGCGTAGAGGAATTAGGCGTTTTAGCAGTAATAGGTAGAGAGAGACATGAATCtggt                                    <  2:515840/136‑1 (MQ=255)
                                                                                                      aTTGTTGCAGGCGCTGGACAAAAAGGTGCCGTTACTATTGCCACTAACATGGCTGGTCGTGGTACAGATATCAAATTAGGTGAATGCGTAGAGGAATTAGGCGGTTTAGCAGTAATAGGTACAGAGCGACATGAATCtcgt                                    <  2:184593/141‑1 (MQ=255)
                                                                                                        tgttgCAGGCGCTGGACAAAAAGGTGCCGTTACTATTGCCACTAACATGGCTGGTCGTGGTACAGATATCAAATTAGGTGAAGGCGTAGAGGAATTAGGCGGTTTAGCAGTAATAGGTACAGAGCGACATGAATCtcgtcg                                  <  1:499749/141‑1 (MQ=255)
                                                                                                         gttgCAGGCGCTGGACAAAAAGGTGCCGTTACTATTGCCACTAACATGGCTGGTCGTGGTACAGATATCAAATTAGGTGAAGGCGTAGAGGAATTAGGCGGTTTAGCAGTAATAGGTACAGAGCGACATGAATCTcgtcgt                                 <  1:478054/141‑1 (MQ=255)
                                                                                                           tgCAGGCGCTGGACAAAAAGGTGCCGTTACTATTGCCACTAACATGGCTGGTCGTGGTACAGATATCAAATTAGGTGAAGGCGTAGAGGAATTAGTGGGTTTAGCAGTAATAGGTACAGAGCGACATGAATCTCGTCGTAt                               >  2:13808/1‑141 (MQ=255)
                                                                                                             cAGGCGCTGGACAAAAAGGTGCCGTTACTATTGCCACTAACGTGTCTGGTCGTGGTCCAGATATCAAATTAGGTGAAGGCGTAGCAGAATCAGGCGGTTTAGCAGTAATAGGTACAGGGCGACATGAATATCGTCGTATtg                             >  2:327134/1‑141 (MQ=255)
                                                                                                                   cTGGACAAAAAGGTGCCGTTACTATTGCCACTAACATGGCTGGTCGTGGTACAGATATCAAATTAGGTGAAGGCGTAGAGGAATTAGGCGGTTTAGCAGTAATAGGTACAGAGCGACATGAATCTCGTCGTATTGGTGAcc                       >  2:78554/1‑141 (MQ=255)
                                                                                                                      gACAAAAAGGTGCCGTTACTATTGCCACTAACATGGCTGGTCGTGGTACAGATATCAAATTAGGTGAAGGCGTAGAGGAATTAGGCGGTTTAGCAGTAATAGGTACAGAGCGACATGAATCTCGTCGTATTGATGACCAGt                    <  2:193973/141‑1 (MQ=255)
                                                                                                                        cAAAAAGGTGCCGTTACTATTGCCACTAACATGGCTGGTCGTGGTACAGATATCAAATTAGGTGAAGGCGTAGAGGAATTAGGCGGTTTAGCAGTAATAGGTACAGAGCGACATGAATCTCGTCGTATTGATGACCAGTTa                  >  1:83904/1‑141 (MQ=255)
                                                                                                                            aaGGTGCCGTTACTATTGCCACTAACATGGCTGGTCGTGGTACAGATATCAAATTAGGTGAAGGCGTAGAGGAATTAGGCGGTTTAGCAGTAATAGGTACAGAGCGACATGAATCTCGTCGTATTGATGACCAGTTACGTg              <  2:194239/141‑1 (MQ=255)
                                                                                                                              ggTGCCGTTACTATTGCCACTAACATGGCTGGTCGTGGTACAGATATCAAATTAGGTGAAGGCGTAGAGGAATTAGGCGGTTTAGCAGTAATAGGTACAGAGCGACATGAATCTCGTCGTATTGATGACCAGTTACGTGGt            >  1:101019/1‑141 (MQ=255)
                                                                                                                                  ccGTTACTATTGCCACTAACATGGCTGGTCGTGGTACAGATATCAAATTAGGTGAAGGCGTAGAGGAATTAGGCGGTTTAGCAGTAATAGGTACAGAGCGACATGAATCTCGTCGTATTGATGACCAGTTACGTTGTGGtt        >  2:85086/1‑141 (MQ=255)
                                                                                                                                  ccGTTACTATTGCCACTAACATGGCTGGTCGTGGTACAGATATCAAATTAGGTGAAGGCGTAGAGGAATTAGGCGGTTTAGCAGTAATAGGTACAGAGCGACATGAATCTCGTCGTATTGATGACCAGTTACGTGGTCGtt        >  2:216107/1‑141 (MQ=255)
                                                                                                                                     ttACTTTTGCCACTAACATGGCTGGTCGTGGTACAGATATCAAATTAGGTGAAGGCGTAGAGGAATTAGGCGGTTTAGCAGTAATAGGTACAGAGCGACATGAATCTCGTCGTATTGATGACCAGTTACGTGGTCGTTCTg     <  1:240775/141‑1 (MQ=255)
                                                                                                                                     ttACTTTTGCCACTAACATGGCTGGTCGTGGTACAGACATCAAATTAGGTGAAGGCGTAGAGGAATTAGGCGGTTTAGCAGTAATAGGTACAGAGCGACATGAATCTCGTCGTATTGATGACCAGTTACGTGGTCGTTCTg     <  1:240866/141‑1 (MQ=255)
                                                                                                                                      tACTATTGCCACTAACATGGCTGGTCGTGGTACAGATATCAAATTAGGTGAAGGCGTAGAGGAATTAGGCGGTTTAGCAGTAATAGGTACAGAGCGACATGAATCTCGTCGTATTGATGACCAGTTACGTGGTCGTTCTgg    <  1:130221/141‑1 (MQ=255)
                                                                                                                                        cTATTGCCACTAACATGGCTGGTCGTGGTACAGATATCAAATTAGGTGAAGGCGTAGAGGAATTAGGCGGTTTAGCAGTAATAGGTACAGAGCGACATGAATCTCGTCGTATTGATGACCAGTTACGTGGTCGTTCTGGAc  <  1:466663/141‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                          
ACTGTTGCAGTTGAGACTTCTGAATATATTTCAAATTTACTTAAAAAACGTGGTATCCGTCATGATGTGTTAAATGCGAAAAATCATGAACGTGAAGCTGAAATTGTTGCAGGCGCTGGACAAAAAGGTGCCGTTACTATTGCCACTAACATGGCTGGTCGTGGTACAGATATCAAATTAGGTGAAGGCGTAGAGGAATTAGGCGGTTTAGCAGTAATAGGTACAGAGCGACATGAATCTCGTCGTATTGATGACCAGTTACGTGGTCGTTCTGGAC  >  CP000730/832568‑832844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: